sidestep | Developmental Studies Hybridoma Bank 9B8 anti-sidestep product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

sidestep

catalog :

9B8 anti-sidestep

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9B8 anti-sidestep

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 2

Reference
Siebert M, Banovic D, Goellner B, Aberle H. Drosophila motor axons recognize and follow a Sidestep-labeled substrate pathway to reach their target fields. Genes Dev. 2009;23:1052-62 pubmed publisher
Sink H, Rehm E, Richstone L, Bulls Y, Goodman C. sidestep encodes a target-derived attractant essential for motor axon guidance in Drosophila. Cell. 2001;105:57-67 pubmed

product information

Internal ID :

3616

Name :

9B8 anti-sidestep

Depositor Name :

Goodman, C.

Depositor Institution :

University of California, Berkeley

Date Deposited :

3/14/02

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

sidestep

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila, others not tested

Initial Publication Pubmed ID :

11301002

Depositor Notes (Special Instructions) :

Works better on fresh rather than MeOH stored embryos, stronger CNS, weaker muscle staining, Best results with use of VectaStain kit .

Collections :

Drosophila antigens,Neurodevelopment

Search Keywords :

Goodman, C., sidestep, Drosophila, MIgG1, Drosophila, side, CG31062, AB_528468, monoclonal, Drosophila antigens/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: multiple isoforms between 100 and 107 kDa; Apparent: around 120 kDa

Gene :

side

Alternate Gene Name(s) :

CG31062

Uniprot ID :

Q9VB35

Antibody Registry ID :

AB_528468

Synonyms (Alternate Clone Names) :

9B8

Immunogen :

Recombinant 6× His-tagged sidestep protein fragment (aa 241–786)

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3 x 63 Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 241–786 (extracellular)

Epitope Map PubMed ID :

11301002

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

11301002

Immunofluorescence Pubmed IDs :

19369411

Western Blot Pubmed IDs :

19369411

DSHB Growth Medium :

RPMI

References (legacy) :

Cell 105, 57-67.

more info or order :

Developmental Studies Hybridoma Bank product webpage