product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Glass
catalog :
9B2.1 anti-glass
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
9B2.1 anti-glass
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 2
Reference
Morrison C, Chen H, Cook T, Brown S, Treisman J. Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye. PLoS Genet. 2018;14:e1007173 pubmed publisher
Ellis M, O Neill E, Rubin G. Expression of Drosophila glass protein and evidence for negative regulation of its activity in non-neuronal cells by another DNA-binding protein. Development. 1993;119:855-65 pubmed
product information
Internal ID :
1618
Name :
9B2.1 anti-glass
Depositor Name :
Rubin, G.M.
Depositor Institution :
University of California - Berkeley
Depositor Website :
http://www.hhmi.org/scientists/gerald-m-rubin
Date Deposited :
8/31/94
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Glass
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Initial Publication Pubmed ID :
8187644
Depositor Notes (Special Instructions) :
9B2.1 Ab recognizes the Drosophila protein glass. Glass is expressed at high levels in the nucleus of the developing photoreceptor cells in a sequence that reflects their acquisition of neuronal identity. 9B2.1 gives strong staining of the morphogenetic furrow and all of the cells that arise from the furrow.
Collections :
Drosophila antigens,Neurodevelopment,Nucleus,Transcription factors
Search Keywords :
Rubin, G.M., Glass, Drosophila, MIgG1, kappa light chain, Drosophila , gl , CG7672 , AB_528268, Monoclonal, Drosophila antigens/Nucleus, Immunohistochemistry/Immunofluorescence/Western blot
Antigen Molecular Weight :
62.5 kDa
Gene :
gl
Alternate Gene Name(s) :
CG7672
Uniprot ID :
P13360
Antibody Registry ID :
AB_528268
Immunogen :
Glass-trp E fusion protein (see PMID: 2010085)
Immunogen Sequence :
Full length sequence
Clonality :
Monoclonal
Myeloma Strain :
P3 x 63Ag8.653
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
8187644
Immunofluorescence Pubmed IDs :
29324767
Additional Information :
RRID: AB_528268. FBgn0004618. Use periodate-lysine-paraformaldehyde (PLP) fix only.
DSHB Growth Medium :
Iscove's
References (legacy) :
Development 119, 855-865.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US