Titin | Developmental Studies Hybridoma Bank 9 D10 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Titin

catalog :

9 D10

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9 D10

reactivity :

jerboas, human, mouse, rat, dogs, chicken, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 46

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:50; loading ...; fig 2e	Selvaraj S, Mondragón González R, Xu B, Magli A, Kim H, Laine J, et al. Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; jerboas; 1:10; loading ...; fig 5b	Tran M, Tsutsumi R, Erberich J, Chen K, Flores M, Cooper K. Evolutionary loss of foot muscle during development with characteristics of atrophy and no evidence of cell death. elife. 2019;8: pubmed publisher
immunocytochemistry; human; 1:10; fig 4	Kim E, Page P, Dellefave Castillo L, McNally E, Wyatt E. Direct reprogramming of urine-derived cells with inducible MyoD for modeling human muscle disease. Skelet Muscle. 2016;6:32 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 3	Rader E, Turk R, Willer T, Beltrán D, Inamori K, Peterson T, et al. Role of dystroglycan in limiting contraction-induced injury to the sarcomeric cytoskeleton of mature skeletal muscle. Proc Natl Acad Sci U S A. 2016;113:10992-7 pubmed publisher
immunocytochemistry; mouse	Yang J, Kaur K, Ong L, Eisenberg C, Eisenberg L. Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors. Stem Cells Int. 2015;2015:270428 pubmed publisher
immunocytochemistry; mouse; fig 9	White J, Barro M, Makarenkova H, Sanger J, Sanger J. Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes. Anat Rec (Hoboken). 2014;297:1571-84 pubmed publisher
western blot; bovine	Lomiwes D, Hurst S, Dobbie P, Frost D, Hurst R, Young O, et al. The protection of bovine skeletal myofibrils from proteolytic damage post mortem by small heat shock proteins. Meat Sci. 2014;97:548-57 pubmed publisher
	Chal J, Al Tanoury Z, Hestin M, Gobert B, Aivio S, Hick A, et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc. 2016;11:1833-50 pubmed publisher
	Chal J, Oginuma M, Al Tanoury Z, Gobert B, Sumara O, Hick A, et al. Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy. Nat Biotechnol. 2015;33:962-9 pubmed publisher
	Can T, Faas L, Ashford D, Dowle A, Thomas J, O Toole P, et al. Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections. Proteome Sci. 2014;12:25 pubmed publisher
	Chen M, Li S, Lam W, Ho Y, Ha S, Chan G, et al. Left ventricular torsional mechanics and myocardial iron load in beta-thalassaemia major: a potential role of titin degradation. BMC Cardiovasc Disord. 2014;14:49 pubmed publisher
	Takahashi M, Yamagishi T, Narematsu M, Kamimura T, Kai M, Nakajima Y. Epicardium is required for sarcomeric maturation and cardiomyocyte growth in the ventricular compact layer mediated by transforming growth factor ? and fibroblast growth factor before the onset of coronary circulation. Congenit Anom (Kyoto). 2014;54:162-71 pubmed publisher
	Alegre Cebollada J, Kosuri P, Giganti D, Eckels E, Rivas Pardo J, Hamdani N, et al. S-glutathionylation of cryptic cysteines enhances titin elasticity by blocking protein folding. Cell. 2014;156:1235-1246 pubmed publisher
	Molnar I, Migh E, Szikora S, Kalmar T, Végh A, Deak F, et al. DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila. PLoS Genet. 2014;10:e1004166 pubmed publisher
	Rosado M, Barber C, Berciu C, Feldman S, Birren S, Nicastro D, et al. Critical roles for multiple formins during cardiac myofibril development and repair. Mol Biol Cell. 2014;25:811-27 pubmed publisher
	Kötter S, Unger A, Hamdani N, Lang P, Vorgerd M, Nagel Steger L, et al. Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins. J Cell Biol. 2014;204:187-202 pubmed publisher
	Grubisic V, Gottipati M, Stout R, Grammer J, Parpura V. Heterogeneity of myotubes generated by the MyoD and E12 basic helix-loop-helix transcription factors in otherwise non-differentiation growth conditions. Biomaterials. 2014;35:2188-98 pubmed publisher
	Nguyen Tran D, Hait N, Sperber H, Qi J, Fischer K, Ieronimakis N, et al. Molecular mechanism of sphingosine-1-phosphate action in Duchenne muscular dystrophy. Dis Model Mech. 2014;7:41-54 pubmed publisher
	Nworu C, Krieg P, Gregorio C. Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging. Methods. 2014;66:370-9 pubmed publisher
	Lee E, De Winter J, Buck D, Jasper J, MALIK F, Labeit S, et al. Fast skeletal muscle troponin activation increases force of mouse fast skeletal muscle and ameliorates weakness due to nebulin-deficiency. PLoS ONE. 2013;8:e55861 pubmed publisher
	Manso A, Li R, Monkley S, Cruz N, Ong S, Lao D, et al. Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload. J Biol Chem. 2013;288:4252-64 pubmed publisher
	Mikelsaar A, Sünter A, Mikelsaar R, Toomik P, Kõiveer A, Mikelsaar I, et al. Epitope of titin A-band-specific monoclonal antibody Tit1 5 H1.1 is highly conserved in several Fn3 domains of the titin molecule. Centriole staining in human, mouse and zebrafish cells. Cell Div. 2012;7:21 pubmed publisher
	Sepac A, Si Tayeb K, Sedlic F, Barrett S, Canfield S, Duncan S, et al. Comparison of cardiomyogenic potential among human ESC and iPSC lines. Cell Transplant. 2012;21:2523-30 pubmed publisher
	Kurosaka S, Leu N, Pavlov I, Han X, Ribeiro P, Xu T, et al. Arginylation regulates myofibrils to maintain heart function and prevent dilated cardiomyopathy. J Mol Cell Cardiol. 2012;53:333-41 pubmed publisher
	Zhang B, Whitehead N, Gervasio O, Reardon T, Vale M, Fatkin D, et al. Pathways of Ca²? entry and cytoskeletal damage following eccentric contractions in mouse skeletal muscle. J Appl Physiol (1985). 2012;112:2077-86 pubmed publisher
	Ottenheijm C, Voermans N, Hudson B, Irving T, Stienen G, van Engelen B, et al. Titin-based stiffening of muscle fibers in Ehlers-Danlos Syndrome. J Appl Physiol (1985). 2012;112:1157-65 pubmed publisher
	Gonçalves M, Janssen J, Nguyen Q, Athanasopoulos T, Hauschka S, Dickson G, et al. Transcription factor rational design improves directed differentiation of human mesenchymal stem cells into skeletal myocytes. Mol Ther. 2011;19:1331-41 pubmed publisher
	Kensah G, Gruh I, Viering J, Schumann H, Dahlmann J, Meyer H, et al. A novel miniaturized multimodal bioreactor for continuous in situ assessment of bioartificial cardiac tissue during stimulation and maturation. Tissue Eng Part C Methods. 2011;17:463-73 pubmed publisher
	Ali M, Cho W, Hudson B, Kassiri Z, Granzier H, Schulz R. Titin is a target of matrix metalloproteinase-2: implications in myocardial ischemia/reperfusion injury. Circulation. 2010;122:2039-47 pubmed publisher
	Karaduleva E, Vikhliantsev I, Podlubnaia Z. [Expression of titin in the myocardium of spontaneously hypertensive rats during in the course of hypertrophy]. Biofizika. 2010;55:612-8 pubmed
	Sanger J, Wang J, Gleason L, Chowrashi P, Dube D, Mittal B, et al. Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules. Cytoskeleton (Hoboken). 2010;67:808-23 pubmed publisher
	Schaart G, Hesselink R, Keizer H, Van Kranenburg G, Drost M, Hesselink M. A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections. Histochem Cell Biol. 2004;122:161-9 pubmed
	Kulke M, Neagoe C, Kolmerer B, Minajeva A, Hinssen H, Bullard B, et al. Kettin, a major source of myofibrillar stiffness in Drosophila indirect flight muscle. J Cell Biol. 2001;154:1045-57 pubmed
	Nwe T, Shimada Y. Inhibition of nebulin and connectin (titin) for assembly of actin filaments during myofibrillogenesis. Tissue Cell. 2000;32:223-7 pubmed
	Greaser M, Wang S, Berri M, Mozdziak P, Kumazawa Y. Sequence and mechanical implications of titin's PEVK region. Adv Exp Med Biol. 2000;481:53-63; discussion 64-6, 107-10 pubmed
	Yang Y, Obinata T, Shimada Y. Developmental relationship of myosin binding proteins (myomesin, connectin and C-protein) to myosin in chicken somites as studied by immunofluorescence microscopy. Cell Struct Funct. 2000;25:177-85 pubmed
	Nwe T, Maruyama K, Shimada Y. Relation of nebulin and connectin (titin) to dynamics of actin in nascent myofibrils of cultured skeletal muscle cells. Exp Cell Res. 1999;252:33-40 pubmed
	Trombitas K, Greaser M, French G, Granzier H. PEVK extension of human soleus muscle titin revealed by immunolabeling with the anti-titin antibody 9D10. J Struct Biol. 1998;122:188-96 pubmed
	Shimada Y, Komiyama M, Begum S, Maruyama K. Development of connectin/titin and nebulin in striated muscles of chicken. Adv Biophys. 1996;33:223-33 pubmed
	Klug M, Soonpaa M, Koh G, Field L. Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts. J Clin Invest. 1996;98:216-24 pubmed
	van der Loop F, Schaart G, Langmann H, Ramaekers F, Viebahn C. Rearrangement of intercellular junctions and cytoskeletal proteins during rabbit myocardium development. Eur J Cell Biol. 1995;68:62-9 pubmed
	van der Loop F, Bosma A, Vos J, Mirck M, Schaart G, van den Ingh T, et al. Cultured pig rhabdomyosarcoma cells with a deletion of the Xq24-qter chromosome region: an immunochemical and cytogenetic characterization. Am J Vet Res. 1995;56:1062-9 pubmed
	Wang S, Greaser M. Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils. J Muscle Res Cell Motil. 1985;6:293-312 pubmed
	Wang S, Greaser M, Schultz E, Bulinski J, Lin J, Lessard J. Studies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin. J Cell Biol. 1988;107:1075-83 pubmed
	Handel S, Wang S, Greaser M, Schultz E, Bulinski J, Lessard J. Skeletal muscle myofibrillogenesis as revealed with a monoclonal antibody to titin in combination with detection of the alpha- and gamma-isoforms of actin. Dev Biol. 1989;132:35-44 pubmed
	Schaart G, Pieper F, Kuijpers H, Bloemendal H, Ramaekers F. Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins. Differentiation. 1991;46:105-15 pubmed

product information

Internal ID :

3688

Name :

9 D10

Depositor Name :

Greaser, M.L.

Depositor Institution :

University of Wisconsin-Madison

Date Deposited :

4/13/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Titin

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Bovine,Canine,Chicken,Drosophila,Fish,Hamster,Human,Mouse,Porcine,Rabbit,Rat,Xenopus

Species Tested (legacy) :

mouse, bovine, human, rat, chick, hamster, zebrafish and rabbit

Initial Publication Pubmed ID :

3905857

Depositor Notes (Special Instructions) :

Stains 4% paraformaldehyde or methanol fixed cells and paraffin sections; also 0.1% glutaraldehyde

Collections :

Cardiac development,Cytoskeleton,Drosophila antigens,Human,Skeletal muscle

Search Keywords :

Greaser, Marion L., Titin, Bovine, MIgM, Mouse/Bovine/Human/Rat/Chicken/Hamster/Rabbit/Xenopus/Drosophila/Canine/Porcine, TTN , , AB_528491, monoclonal, 9d10

Antigen Molecular Weight :

3000-4000 kDa

Gene :

TTN

Uniprot ID :

F1N757

Antibody Registry ID :

AB_528491

Immunogen :

Titin (purified) bovine heart.

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

proline-glutamate-valine-lysine (PEVK) domain;

Epitope Map PubMed ID :

9724620 3905857 10987066 21041693

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

24725620 24360578 21041693 3905857 22626847 8549591

Immunofluorescence Pubmed IDs :

24725620 25125171 24360578 23806641 24430873 24586196 24666202 21041693 22461447 22863088 21266958 23266827 21142417 20886612 15322861 10987066 11037792 10984101 10502397 8690796 2645182 3047149 26237517 27583644 25071420

Western Blot Pubmed IDs :

24630725 22223454 20968071 23437068 3047149

Pubmed IDs :

8922116 8533978 2065865

Additional Information :

single titin molecule labeling PMID: 24357719; immunoelectron microscopy PMID: 9724620 9277364 9172076

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Muscle Res. Cell Motil. 6, 293-312.; J. Cell Biol. 107, 1075-1083.; In "Cellular & Molecular Biology of Muscle Development" UCLA Symp. on Molec. & Cellular Biol. Vol. 93, Liss, A.R. pp. 247-257.; Dev. Biol. 132, 35-44.; Differentiation 46, 105-115.; Am. J. Vet. Res. 56(8), 1062-1069.; Eur. J. Cell Biol. 68, 62-69.; J. Clin. Invest. 98, 216-224.; Adv. Biophys. 33, 223-234.; Cell Struc. & Func. 22, 59-64.; J Muscle Res Cell Motil. 18, 345-351.; Am J Physiol. 273(2 Pt 1), C662-C670.; J Struct. Biol. 122(1-2), 188-196.; Exp. Cell Res. 252, 33-40.; Cell. Struct. Funct. 25, 177-185.; Tissue Cell 32(3), 223-227.; Adv. Exp. Med. Biol. 481, 53-63.; Histochem. Cell Biol. 122, 161-169.; Pol. J. Food Nutr. Sci. 59(4), 315-323.; Cytoskeleton 67, 808-823.; doi:10.1038/nbt.3297

more info or order :

Developmental Studies Hybridoma Bank product webpage