product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Trio
catalog :
9.4A anti-Trio
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
9.4A anti-Trio
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 8
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly; 1:200
Siegenthaler D, Enneking E, Moreno E, Pielage J. L1CAM/Neuroglian controls the axon-axon interactions establishing layered and lobular mushroom body architecture. J Cell Biol. 2015;208:1003-18 pubmed publisher
  • immunohistochemistry; fruit fly; 1:10
Marchetti G, Reichardt I, Knoblich J, Besse F. The TRIM-NHL protein Brat promotes axon maintenance by repressing src64B expression. J Neurosci. 2014;34:13855-64 pubmed publisher
Pézeron G, Millen K, Boukhatmi H, Bray S. Notch directly regulates the cell morphogenesis genes Reck, talin and trio in adult muscle progenitors. J Cell Sci. 2014;127:4634-44 pubmed publisher
Iyer S, Wang D, Iyer E, Trunnell S, Meduri R, Shinwari R, et al. The RhoGEF trio functions in sculpting class specific dendrite morphogenesis in Drosophila sensory neurons. PLoS ONE. 2012;7:e33634 pubmed publisher
Lee S, Thomas G. Rac1 modulation of the apical domain is negatively regulated by β (Heavy)-spectrin. Mech Dev. 2011;128:116-28 pubmed publisher
Ball R, Warren Paquin M, Tsurudome K, Liao E, Elazzouzi F, Cavanagh C, et al. Retrograde BMP signaling controls synaptic growth at the NMJ by regulating trio expression in motor neurons. Neuron. 2010;66:536-49 pubmed publisher
Fushima K, Tsujimura H. Precise control of fasciclin II expression is required for adult mushroom body development in Drosophila. Dev Growth Differ. 2007;49:215-27 pubmed
Awasaki T, Saito M, Sone M, Suzuki E, Sakai R, Ito K, et al. The Drosophila trio plays an essential role in patterning of axons by regulating their directional extension. Neuron. 2000;26:119-31 pubmed
product information
Internal ID :
4101
Name :
9.4A anti-Trio
Depositor Name :
Hama, C.
Depositor Institution :
Faculty of Life Sciences, Kyoto Sangyo University
Date Deposited :
6/20/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Trio
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
10798397
Depositor Notes (Special Instructions) :
9.4A stains the gamma and alpha’/beta’ lobes of mushroom body.
Collections :
Drosophila antigens,Enzymes,Neurodevelopment
Search Keywords :
Hama, C., Trio, Drosophila, MIgG1, Drosophila, trio, CG18214, AB_528494, monoclonal, Drosophila antigens/Enzymes/Neurodevelopment, Immunofluorescence/Western blot
Antigen Molecular Weight :
Multiple isoforms; Predicted: 46, 73, 88, and 257 kDa; Apparent: 130 and 257 kDa
Gene :
trio
Alternate Gene Name(s) :
CG18214
Uniprot ID :
Q7KVD1
Antibody Registry ID :
AB_528494
Immunogen :
Recombinant Trio (aa 1697–1925 of isoform A)
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Myeloma Strain :
P3-X63-Ag8-U1
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 1697–1925 of isoform A
Epitope Map PubMed ID :
10798397
Recommended Applications :
Immunofluorescence,Western Blot
Immunofluorescence Pubmed IDs :
10798397 25825519 25297111 25217625 20510858 22442703 17394600 21111816
Western Blot Pubmed IDs :
20510858
Additional Information :
This antibody should recognize isoforms A and D and may recognize isoforms B and E.
DSHB Growth Medium :
RPMI
References (legacy) :
Neuron 26, 119-131.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US