product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Repo; Reversed polarity protein
catalog :
8D12 anti-Repo
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8D12 anti-Repo
reactivity :
Drosophila melanogaster
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 70
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Drosophila melanogaster; 1:20; loading ...; fig 2s5a
DELVENTHAL R, O Connor R, Pantalia M, Ulgherait M, Kim H, Basturk M, et al. Dissection of central clock function in Drosophila through cell-specific CRISPR-mediated clock gene disruption. elife. 2019;8: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:100; loading ...; fig 1s1c
Peng J, Lin S, Liu Y, Lin H, Li T, Yao C. A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the Drosophila motor system. elife. 2019;8: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:25; fig 6d
Weiss S, Melom J, Ormerod K, Zhang Y, Littleton J. Glial Ca2+signaling links endocytosis to K+ buffering around neuronal somas to regulate excitability. elife. 2019;8: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; loading ...; fig 1a
Chen X, Wanggou S, Bodalia A, Zhu M, Dong W, Fan J, et al. A Feedforward Mechanism Mediated by Mechanosensitive Ion Channel PIEZO1 and Tissue Mechanics Promotes Glioma Aggression. Neuron. 2018;100:799-815.e7 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:20; loading ...; fig 1e
Foo L, Song S, Cohen S. miR-31 mutants reveal continuous glial homeostasis in the adult Drosophila brain. EMBO J. 2017;36:1215-1226 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:20; loading ...; fig 2j
Gerdøe Kristensen S, Lund V, Wandall H, Kjaerulff O. Mactosylceramide prevents glial cell overgrowth by inhibiting insulin and fibroblast growth factor receptor signaling. J Cell Physiol. 2017;232:3112-3127 pubmed publisher
  • immunohistochemistry - frozen section; Drosophila melanogaster; 1:100; fig 2
Li T, Giagtzoglou N, Eberl D, Jaiswal S, Cai T, Godt D, et al. The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals. elife. 2016;5: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:10; fig 5
Ma J, Brennan K, D Aloia M, Pascuzzi P, Weake V. Transcriptome Profiling Identifies Multiplexin as a Target of SAGA Deubiquitinase Activity in Glia Required for Precise Axon Guidance During Drosophila Visual Development. G3 (Bethesda). 2016;6:2435-45 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:10; fig 4A
Lee S, Bang S, Hong Y, Lee J, Jeong H, Park S, et al. The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease. Dis Model Mech. 2016;9:295-306 pubmed publisher
  • immunocytochemistry; Drosophila melanogaster; 1:20
Strauss A, Kawasaki F, Ordway R. A Distinct Perisynaptic Glial Cell Type Forms Tripartite Neuromuscular Synapses in the Drosophila Adult. PLoS ONE. 2015;10:e0129957 pubmed publisher
  • immunohistochemistry - paraffin section; Drosophila melanogaster; 1:25
Hegde V, Vogel R, Feany M. Glia are critical for the neuropathology of complex I deficiency in Drosophila. Hum Mol Genet. 2014;23:4686-92 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:10
Levendusky M, Basle J, Chang S, Mandalaywala N, Voigt J, DEARBORN R. Expression and regulation of vitamin D3 upregulated protein 1 (VDUP1) is conserved in mammalian and insect brain. J Comp Neurol. 2009;517:581-600 pubmed publisher
Ojelade S, Lee T, Giagtzoglou N, Yu L, Ugur B, Li Y, et al. cindr, the Drosophila Homolog of the CD2AP Alzheimer's Disease Risk Gene, Is Required for Synaptic Transmission and Proteostasis. Cell Rep. 2019;28:1799-1813.e5 pubmed publisher
Sengupta S, Crowe L, You S, Roberts M, Jackson F. A Secreted Ig-Domain Protein Required in Both Astrocytes and Neurons for Regulation of Drosophila Night Sleep. Curr Biol. 2019;29:2547-2554.e2 pubmed publisher
Kanca O, Andrews J, Lee P, Patel C, Braddock S, Slavotinek A, et al. De Novo Variants in WDR37 Are Associated with Epilepsy, Colobomas, Dysmorphism, Developmental Delay, Intellectual Disability, and Cerebellar Hypoplasia. Am J Hum Genet. 2019;105:413-424 pubmed publisher
Politano S, Salemme R, Ashley J, López Rivera J, Bakula T, Puhalla K, et al. Tao Negatively Regulates BMP Signaling During Neuromuscular Junction Development in Drosophila. Dev Neurobiol. 2019;79:335-349 pubmed publisher
Krouwels A, Melchels F, Van Rijen M, Ten Brink C, Dhert W, Cumhur Öner F, et al. Focal adhesion signaling affects regeneration by human nucleus pulposus cells in collagen- but not carbohydrate-based hydrogels. Acta Biomater. 2018;66:238-247 pubmed publisher
Baker R, Nakamura N, Chandel I, Howell B, Lyalin D, Panin V. Protein O-Mannosyltransferases Affect Sensory Axon Wiring and Dynamic Chirality of Body Posture in the Drosophila Embryo. J Neurosci. 2018;38:1850-1865 pubmed publisher
Toegel M, Azzam G, Lee E, Knapp D, Tan Y, Fa M, et al. A multiplexable TALE-based binary expression system for in vivo cellular interaction studies. Nat Commun. 2017;8:1663 pubmed publisher
Hindle S, Hebbar S, Schwudke D, Elliott C, Sweeney S. A saposin deficiency model in Drosophila: Lysosomal storage, progressive neurodegeneration and sensory physiological decline. Neurobiol Dis. 2017;98:77-87 pubmed publisher
Tofangchi A, Fan A, Saif M. Mechanism of Axonal Contractility in Embryonic Drosophila Motor Neurons In Vivo. Biophys J. 2016;111:1519-1527 pubmed publisher
Tsao C, Ku H, Lee Y, Huang Y, Sun Y. Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs. PLoS ONE. 2016;11:e0163744 pubmed publisher
Hess Homeier D, Fan C, Gupta T, Chiang A, Certel S. Astrocyte-specific regulation of hMeCP2 expression in Drosophila. Biol Open. 2014;3:1011-9 pubmed publisher
Perez E, Das G, Bergmann A, Baehrecke E. Autophagy regulates tissue overgrowth in a context-dependent manner. Oncogene. 2015;34:3369-76 pubmed publisher
Muthukumar A, Stork T, Freeman M. Activity-dependent regulation of astrocyte GAT levels during synaptogenesis. Nat Neurosci. 2014;17:1340-50 pubmed publisher
Xie X, GILBERT M, Petley Ragan L, Auld V. Loss of focal adhesions in glia disrupts both glial and photoreceptor axon migration in the Drosophila visual system. Development. 2014;141:3072-83 pubmed publisher
Stork T, Sheehan A, Tasdemir Yilmaz O, Freeman M. Neuron-glia interactions through the Heartless FGF receptor signaling pathway mediate morphogenesis of Drosophila astrocytes. Neuron. 2014;83:388-403 pubmed publisher
Meyer S, Schmidt I, Klämbt C. Glia ECM interactions are required to shape the Drosophila nervous system. Mech Dev. 2014;133:105-16 pubmed publisher
Luo J, Liu Y, Nassel D. Insulin/IGF-regulated size scaling of neuroendocrine cells expressing the bHLH transcription factor Dimmed in Drosophila. PLoS Genet. 2013;9:e1004052 pubmed publisher
Tasdemir Yilmaz O, Freeman M. Astrocytes engage unique molecular programs to engulf pruned neuronal debris from distinct subsets of neurons. Genes Dev. 2014;28:20-33 pubmed publisher
Ghosh A, Kling T, Snaidero N, Sampaio J, Shevchenko A, Gras H, et al. A global in vivo Drosophila RNAi screen identifies a key role of ceramide phosphoethanolamine for glial ensheathment of axons. PLoS Genet. 2013;9:e1003980 pubmed publisher
Yang Y, Hou L, Li Y, Ni J, Liu L. Neuronal necrosis and spreading death in a Drosophila genetic model. Cell Death Dis. 2013;4:e723 pubmed publisher
Viktorin G, Riebli N, Reichert H. A multipotent transit-amplifying neuroblast lineage in the central brain gives rise to optic lobe glial cells in Drosophila. Dev Biol. 2013;379:182-94 pubmed publisher
Hopkins P. Neurodegeneration in a Drosophila model for the function of TMCC2, an amyloid protein precursor-interacting and apolipoprotein E-binding protein. PLoS ONE. 2013;8:e55810 pubmed publisher
Kallijärvi J, Stratoulias V, Virtanen K, Hietakangas V, Heino T, Saarma M. Characterization of Drosophila GDNF receptor-like and evidence for its evolutionarily conserved interaction with neural cell adhesion molecule (NCAM)/FasII. PLoS ONE. 2012;7:e51997 pubmed publisher
Laneve P, Delaporte C, Trebuchet G, Komonyi O, Flici H, Popkova A, et al. The Gcm/Glide molecular and cellular pathway: new actors and new lineages. Dev Biol. 2013;375:65-78 pubmed publisher
Kapfhamer D, King I, Zou M, Lim J, Heberlein U, Wolf F. JNK pathway activation is controlled by Tao/TAOK3 to modulate ethanol sensitivity. PLoS ONE. 2012;7:e50594 pubmed publisher
Sone M, Zeng X, Larese J, Ryoo H. A modified UPR stress sensing system reveals a novel tissue distribution of IRE1/XBP1 activity during normal Drosophila development. Cell Stress Chaperones. 2013;18:307-19 pubmed publisher
Hara Y, Hirai K, Togane Y, Akagawa H, Iwabuchi K, Tsujimura H. Ecdysone-dependent and ecdysone-independent programmed cell death in the developing optic lobe of Drosophila. Dev Biol. 2013;374:127-41 pubmed publisher
Ziegler A, Brüsselbach F, Hovemann B. Activity and coexpression of Drosophila black with ebony in fly optic lobes reveals putative cooperative tasks in vision that evade electroretinographic detection. J Comp Neurol. 2013;521:1207-24 pubmed publisher
Berger C, Harzer H, Burkard T, Steinmann J, van der Horst S, Laurenson A, et al. FACS purification and transcriptome analysis of drosophila neural stem cells reveals a role for Klumpfuss in self-renewal. Cell Rep. 2012;2:407-18 pubmed publisher
Brink D, GILBERT M, Xie X, Petley Ragan L, Auld V. Glial processes at the Drosophila larval neuromuscular junction match synaptic growth. PLoS ONE. 2012;7:e37876 pubmed publisher
Li Y, Padgett R. bantam is required for optic lobe development and glial cell proliferation. PLoS ONE. 2012;7:e32910 pubmed publisher
Edwards T, Nuschke A, Nern A, Meinertzhagen I. Organization and metamorphosis of glia in the Drosophila visual system. J Comp Neurol. 2012;520:2067-85 pubmed publisher
Jiang Y, Reichert H. Programmed cell death in type II neuroblast lineages is required for central complex development in the Drosophila brain. Neural Dev. 2012;7:3 pubmed publisher
Fujioka M, Jaynes J. Regulation of a duplicated locus: Drosophila sloppy paired is replete with functionally overlapping enhancers. Dev Biol. 2012;362:309-19 pubmed publisher
Quijano J, Stinchfield M, Newfeld S. Wg signaling via Zw3 and mad restricts self-renewal of sensory organ precursor cells in Drosophila. Genetics. 2011;189:809-24 pubmed publisher
Xie X, Auld V. Integrins are necessary for the development and maintenance of the glial layers in the Drosophila peripheral nerve. Development. 2011;138:3813-22 pubmed publisher
Nakayama M, Sato H, Okuda T, Fujisawa N, Kono N, Arai H, et al. Drosophila carrying pex3 or pex16 mutations are models of Zellweger syndrome that reflect its symptoms associated with the absence of peroxisomes. PLoS ONE. 2011;6:e22984 pubmed publisher
Viktorin G, Riebli N, Popkova A, Giangrande A, Reichert H. Multipotent neural stem cells generate glial cells of the central complex through transit amplifying intermediate progenitors in Drosophila brain development. Dev Biol. 2011;356:553-65 pubmed publisher
Demilly A, Simionato E, Ohayon D, Kerner P, Garcès A, Vervoort M. Coe genes are expressed in differentiating neurons in the central nervous system of protostomes. PLoS ONE. 2011;6:e21213 pubmed publisher
Fore T, Ojwang A, Warner M, Peng X, Bohm R, Welch W, et al. Mapping and application of enhancer-trap flippase expression in larval and adult Drosophila CNS. J Vis Exp. 2011;: pubmed publisher
Mast F, Li J, Virk M, Hughes S, Simmonds A, Rachubinski R. A Drosophila model for the Zellweger spectrum of peroxisome biogenesis disorders. Dis Model Mech. 2011;4:659-72 pubmed publisher
Chu LaGraff Q, Blanchette C, O Hern P, Denefrio C. The Batten disease Palmitoyl Protein Thioesterase 1 gene regulates neural specification and axon connectivity during Drosophila embryonic development. PLoS ONE. 2010;5:e14402 pubmed publisher
Chell J, Brand A. Nutrition-responsive glia control exit of neural stem cells from quiescence. Cell. 2010;143:1161-73 pubmed publisher
Mosca T, Schwarz T. Drosophila Importin-?2 is involved in synapse, axon and muscle development. PLoS ONE. 2010;5:e15223 pubmed publisher
Leiserson W, Forbush B, Keshishian H. Drosophila glia use a conserved cotransporter mechanism to regulate extracellular volume. Glia. 2011;59:320-32 pubmed publisher
Yasugi T, Sugie A, Umetsu D, Tabata T. Coordinated sequential action of EGFR and Notch signaling pathways regulates proneural wave progression in the Drosophila optic lobe. Development. 2010;137:3193-203 pubmed publisher
Ngo K, Wang J, Junker M, Kriz S, Vo G, Asem B, et al. Concomitant requirement for Notch and Jak/Stat signaling during neuro-epithelial differentiation in the Drosophila optic lobe. Dev Biol. 2010;346:284-95 pubmed publisher
Ruiz S, Rickert C, Berger C, Technau G, Cantera R. Spatio-temporal pattern of cells expressing the clock genes period and timeless and the lineages of period expressing neurons in the embryonic CNS of Drosophila melanogaster. Gene Expr Patterns. 2010;10:274-82 pubmed publisher
Cardona A, Saalfeld S, Arganda I, Pereanu W, Schindelin J, Hartenstein V. Identifying neuronal lineages of Drosophila by sequence analysis of axon tracts. J Neurosci. 2010;30:7538-53 pubmed publisher
ROBINETT C, Vaughan A, Knapp J, Baker B. Sex and the single cell. II. There is a time and place for sex. PLoS Biol. 2010;8:e1000365 pubmed publisher
Potter C, Tasic B, Russler E, Liang L, Luo L. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. Cell. 2010;141:536-48 pubmed publisher
Hebbar S, Fernandes J. Glial remodeling during metamorphosis influences the stabilization of motor neuron branches in Drosophila. Dev Biol. 2010;340:344-54 pubmed publisher
Monastirioti M, Giagtzoglou N, Koumbanakis K, Zacharioudaki E, Deligiannaki M, Wech I, et al. Drosophila Hey is a target of Notch in asymmetric divisions during embryonic and larval neurogenesis. Development. 2010;137:191-201 pubmed publisher
Klein Y, Halachmi N, Egoz Matia N, Toder M, Salzberg A. The proprioceptive and contractile systems in Drosophila are both patterned by the EGR family transcription factor Stripe. Dev Biol. 2010;337:458-70 pubmed publisher
Munro M, Akkam Y, Curtin K. Mutational analysis of Drosophila basigin function in the visual system. Gene. 2010;449:50-8 pubmed publisher
Read R, Cavenee W, Furnari F, Thomas J. A drosophila model for EGFR-Ras and PI3K-dependent human glioma. PLoS Genet. 2009;5:e1000374 pubmed publisher
Watson M, Lagow R, Xu K, Zhang B, Bonini N. A drosophila model for amyotrophic lateral sclerosis reveals motor neuron damage by human SOD1. J Biol Chem. 2008;283:24972-81 pubmed publisher
Alfonso T, Jones B. gcm2 promotes glial cell differentiation and is required with glial cells missing for macrophage development in Drosophila. Dev Biol. 2002;248:369-83 pubmed
product information
Internal ID :
3610
Name :
8D12 anti-Repo
Depositor Name :
Goodman, C.
Depositor Institution :
University of California, Berkeley
Date Deposited :
3/14/02
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Repo; Reversed polarity protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila, others not tested
Initial Publication Pubmed ID :
12167411
Depositor Notes (Special Instructions) :
Excellent marker for glial cells; stains the nuclei of all glia, except for the midline glia
Collections :
Cell signaling,Drosophila antigens,Transcription factors
Search Keywords :
Goodman, Corey, Repo; Reversed polarity protein, Drosophila, MIgG1, kappa light chain, Drosophila, Repo, AbRK2; Repo; REPO; rk2; RK2, AB_528448, monoclonal
Gene :
Repo
Alternate Gene Name(s) :
AbRK2; Repo; REPO; rk2; RK2
Uniprot ID :
Q7KSE4
Antibody Registry ID :
AB_528448
Immunogen :
Repo (a.a. 218-612)-6Xhistidine fusion protein.
Clonality :
Monoclonal
Myeloma Strain :
P3 x 63 Ag8.653
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunohistochemistry Pubmed IDs :
20434990 25033182 21183078 25053436 12167411 24859129 20519528 24760769 25174403 21673643 23124681 23409049 23227189 22257485 20558325 21151903 22351615 19782733 21828098 21203506 20079727 23160805 19944090 24348263 20040486 23284846 20724446 21708145 21826223 21695052 22884370 23628691 24385933 23149076 23846225 21868604 20692248 23276603 22178246 29174589 29162808 31353186 31412248
Immunofluorescence Pubmed IDs :
25151265 25033182 21183078 25053436 24361692 24859129 24760769 25174403 21673643 21125654 23124681 23409049 23227189 22257485 21669930 20558325 21151903 22351615 22412948 21828098 20079727 23160805 19944090 24348263 20040486 20454565 21708145 21695052 22884370 23628691 24385933 23149076 23846225 22666403 23276603 27685172 27705774 29167399 27913291 31002474 31327508 31327508 31318331
DSHB Growth Medium :
RPMI
References (legacy) :
Dev. Biol. 248(2), 369-383.; Cell 141(3), 536-548.; Dev. Biol. 362, 309-319.; Dev. Biol. 375, 65-78.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US