Syntaxin | Developmental Studies Hybridoma Bank 8C3 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Syntaxin

catalog :

8C3

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8C3

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 53

Published Application/Species/Sample/Dilution	Reference
western blot; fruit fly ; 1:500; loading ...; fig 5o	Pandey M, Bansal S, Bar S, Yadav A, Sokol N, Tennessen J, et al. miR-125-chinmo pathway regulates dietary restriction-dependent enhancement of lifespan in Drosophila. elife. 2021;10: pubmed publisher
western blot; fruit fly ; 1:1000	Trivedi D, Cm V, Bisht K, Janardan V, Pandit A, Basak B, et al. A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling. elife. 2020;9: pubmed publisher
immunohistochemistry; fruit fly ; 1:100; loading ...; fig 12c western blot; fruit fly ; 1:1000; loading ...; fig 1e	Guan Z, Quiñones Frías M, Akbergenova Y, Littleton J. Drosophila Synaptotagmin 7 negatively regulates synaptic vesicle release and replenishment in a dosage-dependent manner. elife. 2020;9: pubmed publisher
western blot; fruit fly ; 1:500; loading ...; fig 1s1b	Weiss S, Melom J, Ormerod K, Zhang Y, Littleton J. Glial Ca2+signaling links endocytosis to K+ buffering around neuronal somas to regulate excitability. elife. 2019;8: pubmed publisher
western blot; fruit fly ; 1:1000; loading ...; fig 6d	Bangi E, Murgia C, Teague A, Sansom O, Cagan R. Functional exploration of colorectal cancer genomes using Drosophila. Nat Commun. 2016;7:13615 pubmed publisher
immunoprecipitation; fruit fly ; fig 5	Gradilla A, Gonzalez E, Seijo I, Andres G, Bischoff M, González Méndez L, et al. Exosomes as Hedgehog carriers in cytoneme-mediated transport and secretion. Nat Commun. 2014;5:5649 pubmed publisher
immunocytochemistry; fruit fly ; 1:100	Li L, Tian X, Zhu M, Bulgari D, Böhme M, Goettfert F, et al. Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons. J Neurosci. 2014;34:8474-87 pubmed publisher
	Won J, Kim G, Kim J, Cho D, Kwon B, Bae Y, et al. ADAMTS Sol narae cleaves extracellular Wingless to generate a novel active form that regulates cell proliferation in Drosophila. Cell Death Dis. 2019;10:564 pubmed publisher
	Langellotti S, Romano V, Romano G, Klima R, Feiguin F, Cragnaz L, et al. A novel Drosophila model of TDP-43 proteinopathies: N-terminal sequences combined with the Q/N domain induce protein functional loss and locomotion defects. Dis Model Mech. 2016;9:659-69 pubmed publisher
	Rosenbaum E, Vasiljevic E, Cleland S, Flores C, Colley N. The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival. J Biol Chem. 2014;289:32392-409 pubmed publisher
	Gagliardi M, Hernandez A, McGough I, Vincent J. Inhibitors of endocytosis prevent Wnt/Wingless signalling by reducing the level of basal β-catenin/Armadillo. J Cell Sci. 2014;127:4918-26 pubmed publisher
	Iacobucci G, Rahman N, Valtueña A, Nayak T, Gunawardena S. Spatial and temporal characteristics of normal and perturbed vesicle transport. PLoS ONE. 2014;9:e97237 pubmed publisher
	Wang D, Epstein D, Khalaf O, Srinivasan S, Williamson W, Fayyazuddin A, et al. Ca2+-Calmodulin regulates SNARE assembly and spontaneous neurotransmitter release via v-ATPase subunit V0a1. J Cell Biol. 2014;205:21-31 pubmed publisher
	Liu Z, Huang Y, Hu W, Huang S, Wang Q, Han J, et al. dAcsl, the Drosophila ortholog of acyl-CoA synthetase long-chain family member 3 and 4, inhibits synapse growth by attenuating bone morphogenetic protein signaling via endocytic recycling. J Neurosci. 2014;34:2785-96 pubmed publisher
	Lalle M, Leptourgidou F, Camerini S, Pozio E, Skoulakis E. Interkingdom complementation reveals structural conservation and functional divergence of 14-3-3 proteins. PLoS ONE. 2013;8:e78090 pubmed publisher
	Iyer J, Wahlmark C, Kuser Ahnert G, Kawasaki F. Molecular mechanisms of COMPLEXIN fusion clamp function in synaptic exocytosis revealed in a new Drosophila mutant. Mol Cell Neurosci. 2013;56:244-54 pubmed publisher
	Khuong T, Habets R, Kuenen S, Witkowska A, Kasprowicz J, Swerts J, et al. Synaptic PI(3,4,5)P3 is required for Syntaxin1A clustering and neurotransmitter release. Neuron. 2013;77:1097-108 pubmed publisher
	VoSSfeldt H, Butzlaff M, PrüSSing K, Ní Chárthaigh R, Karsten P, Lankes A, et al. Large-scale screen for modifiers of ataxin-3-derived polyglutamine-induced toxicity in Drosophila. PLoS ONE. 2012;7:e47452 pubmed publisher
	Reis G, Yang G, Szpankowski L, Weaver C, Shah S, Robinson J, et al. Molecular motor function in axonal transport in vivo probed by genetic and computational analysis in Drosophila. Mol Biol Cell. 2012;23:1700-14 pubmed publisher
	Haberman A, Williamson W, Epstein D, Wang D, Rina S, Meinertzhagen I, et al. The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration. J Cell Biol. 2012;196:261-76 pubmed publisher
	Tian X, Li J, Valakh V, DiAntonio A, Wu C. Drosophila Rae1 controls the abundance of the ubiquitin ligase Highwire in post-mitotic neurons. Nat Neurosci. 2011;14:1267-75 pubmed publisher
	Shandala T, Woodcock J, Ng Y, Biggs L, Skoulakis E, Brooks D, et al. Drosophila 14-3-3ε has a crucial role in anti-microbial peptide secretion and innate immunity. J Cell Sci. 2011;124:2165-74 pubmed publisher
	Yu W, Kawasaki F, Ordway R. Activity-dependent interactions of NSF and SNAP at living synapses. Mol Cell Neurosci. 2011;47:19-27 pubmed publisher
	Tiklová K, Senti K, Wang S, Gräslund A, Samakovlis C. Epithelial septate junction assembly relies on melanotransferrin iron binding and endocytosis in Drosophila. Nat Cell Biol. 2010;12:1071-7 pubmed publisher
	Diao F, Chaufty J, Waro G, Tsunoda S. SIDL interacts with the dendritic targeting motif of Shal (K(v)4) K+ channels in Drosophila. Mol Cell Neurosci. 2010;45:75-83 pubmed publisher
	Kosmidis S, Grammenoudi S, Papanikolopoulou K, Skoulakis E. Differential effects of Tau on the integrity and function of neurons essential for learning in Drosophila. J Neurosci. 2010;30:464-77 pubmed publisher
	Messaritou G, Grammenoudi S, Skoulakis E. Dimerization is essential for 14-3-3zeta stability and function in vivo. J Biol Chem. 2010;285:1692-700 pubmed publisher
	Wairkar Y, Toda H, Mochizuki H, Furukubo Tokunaga K, Tomoda T, DiAntonio A. Unc-51 controls active zone density and protein composition by downregulating ERK signaling. J Neurosci. 2009;29:517-28 pubmed publisher
	Hebbar S, Lee E, Manna M, Steinert S, Kumar G, Wenk M, et al. A fluorescent sphingolipid binding domain peptide probe interacts with sphingolipids and cholesterol-dependent raft domains. J Lipid Res. 2008;49:1077-89 pubmed publisher
	Zeng X, Sun M, Liu L, Chen F, Wei L, Xie W. Neurexin-1 is required for synapse formation and larvae associative learning in Drosophila. FEBS Lett. 2007;581:2509-16 pubmed
	Haghnia M, Cavalli V, Shah S, Schimmelpfeng K, Brusch R, Yang G, et al. Dynactin is required for coordinated bidirectional motility, but not for dynein membrane attachment. Mol Biol Cell. 2007;18:2081-9 pubmed
	Lagow R, Bao H, Cohen E, Daniels R, Zuzek A, Williams W, et al. Modification of a hydrophobic layer by a point mutation in syntaxin 1A regulates the rate of synaptic vesicle fusion. PLoS Biol. 2007;5:e72 pubmed
	Coutelis J, Ephrussi A. Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis. Development. 2007;134:1419-30 pubmed
	Wang X, Shaw W, Tsang H, Reid E, O Kane C. Drosophila spichthyin inhibits BMP signaling and regulates synaptic growth and axonal microtubules. Nat Neurosci. 2007;10:177-85 pubmed
	Grammenoudi S, Kosmidis S, Skoulakis E. Cell type-specific processing of human Tau proteins in Drosophila. FEBS Lett. 2006;580:4602-6 pubmed
	Pereanu W, Hartenstein V. Neural lineages of the Drosophila brain: a three-dimensional digital atlas of the pattern of lineage location and projection at the late larval stage. J Neurosci. 2006;26:5534-53 pubmed
	Huang F, Woodruff E, Mohrmann R, Broadie K. Rolling blackout is required for synaptic vesicle exocytosis. J Neurosci. 2006;26:2369-79 pubmed
	Hiesinger P, Fayyazuddin A, Mehta S, Rosenmund T, Schulze K, Zhai R, et al. The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila. Cell. 2005;121:607-620 pubmed publisher
	Shimizu H, Kawamura S, Ozaki K. An essential role of Rab5 in uniformity of synaptic vesicle size. J Cell Sci. 2003;116:3583-90 pubmed
	Xue M, Zhang B. Do SNARE proteins confer specificity for vesicle fusion?. Proc Natl Acad Sci U S A. 2002;99:13359-61 pubmed
	Vilinsky I, Stewart B, Drummond J, Robinson I, Deitcher D. A Drosophila SNAP-25 null mutant reveals context-dependent redundancy with SNAP-24 in neurotransmission. Genetics. 2002;162:259-71 pubmed
	Alfonso T, Jones B. gcm2 promotes glial cell differentiation and is required with glial cells missing for macrophage development in Drosophila. Dev Biol. 2002;248:369-83 pubmed
	Rao S, Stewart B, Rivlin P, Vilinsky I, Watson B, Lang C, et al. Two distinct effects on neurotransmission in a temperature-sensitive SNAP-25 mutant. EMBO J. 2001;20:6761-71 pubmed
	Mohtashami M, Stewart B, Boulianne G, Trimble W. Analysis of the mutant Drosophila N-ethylmaleimide sensitive fusion-1 protein in comatose reveals molecular correlates of the behavioural paralysis. J Neurochem. 2001;77:1407-17 pubmed
	Hiesinger P, Scholz M, Meinertzhagen I, Fischbach K, Obermayer K. Visualization of synaptic markers in the optic neuropils of Drosophila using a new constrained deconvolution method. J Comp Neurol. 2001;429:277-88 pubmed
	Stewart B, Mohtashami M, Trimble W, Boulianne G. SNARE proteins contribute to calcium cooperativity of synaptic transmission. Proc Natl Acad Sci U S A. 2000;97:13955-60 pubmed
	Nie Z, Ranjan R, Wenniger J, Hong S, Bronk P, Zinsmaier K. Overexpression of cysteine-string proteins in Drosophila reveals interactions with syntaxin. J Neurosci. 1999;19:10270-9 pubmed
	Wu M, Fergestad T, Lloyd T, He Y, Broadie K, Bellen H. Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo. Neuron. 1999;23:593-605 pubmed
	Tolar L, Pallanck L. NSF function in neurotransmitter release involves rearrangement of the SNARE complex downstream of synaptic vesicle docking. J Neurosci. 1998;18:10250-6 pubmed
	Littleton J, Chapman E, Kreber R, Garment M, Carlson S, Ganetzky B. Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassembly. Neuron. 1998;21:401-13 pubmed
	Burgess R, Deitcher D, Schwarz T. The synaptic protein syntaxin1 is required for cellularization of Drosophila embryos. J Cell Biol. 1997;138:861-75 pubmed
	Schulze K, Bellen H. Drosophila syntaxin is required for cell viability and may function in membrane formation and stabilization. Genetics. 1996;144:1713-24 pubmed
	Fujita S, Zipursky S, Benzer S, Ferrus A, Shotwell S. Monoclonal antibodies against the Drosophila nervous system. Proc Natl Acad Sci U S A. 1982;79:7929-33 pubmed

product information

Internal ID :

802

Name :

8C3

Depositor Name :

Benzer, S. / Colley, N.

Depositor Institution :

California Institute of Technology

Date Deposited :

8/27/98

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Syntaxin

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

6818557

Collections :

Cell signaling,Drosophila antigens

Search Keywords :

Benzer, Seymour / Colley, Nansi, Syntaxin , Drosophila, MIgG1, kappa light chain, Drosophila, Syx1A, Dm Syx1; DmSyx1A; dsyn-1A; dSyx1; Syt1A;, AB_528484, monoclonal

Antigen Molecular Weight :

35 kDa

Gene :

Syx1A

Alternate Gene Name(s) :

Dm Syx1; DmSyx1A; dsyn-1A; dSyx1; Syt1A;

Uniprot ID :

Q24547

Antibody Registry ID :

AB_528484

Immunogen :

Heads(Drosophila melanogaster), homogenized.

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

6818557 17220882 8978057 11116220 24878565 24733584 24948803 23522045 16707805 17329360 12374848 12242238 9265652

Immunofluorescence Pubmed IDs :

11116220 24948803 21670199 23522045 17329360 17498701 27101846

Western Blot Pubmed IDs :

12167411 24553921 27101846 25261468 25236598 21670199 22398725 20071510 20935638 23769723 19920133 20550966 15907473 22270918 21874015 24147113 23139745 21316453 17341138 16875690 17360970 16510714 17498701 18263852 11726512 12876219 11095753 19144852 11389191 10575024 9728921 9852562 9265652 31332194

Immunoprecipitation Pubmed IDs :

15907473 24733584 22270918 21316453 10433270 10575024

DSHB Growth Medium :

RPMI

References (legacy) :

Proc. Natl. Acad. Sci. USA 79, 7929-7933.; Genetics 144, 1713.; J. Comp. Neurol. 429, 277-288.; Nat. Neurosci. 10(2), 177-185.

more info or order :

Developmental Studies Hybridoma Bank product webpage