cadherin, E- | Developmental Studies Hybridoma Bank 8C2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

cadherin, E-

catalog :

8C2

clonality :

monoclonal

host :

house mouse

conjugate :

nonconjugated

clone name :

8C2

reactivity :

Xenopus laevis

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Reference
Lear P, Jayanthi N, Teague W, Johnson P. Foregut Mesenchyme Contributes Cells to Islets during Pancreatic Development in a 3-Dimensional Avian Model. Organogenesis. 2004;1:45-51 pubmed
Teague W, Rowan Hull A, Johnson P. Pancreatic alpha-cell differentiation by mesenchyme-to-epithelial transition: implications for cell-based therapies in children. J Pediatr Surg. 2007;42:153-9 pubmed
Teague W, Rowan Hull A, Jayanthi N, Johnson P. The competency of foregut mesenchyme in islet mesenchyme-to-epithelial transition during embryonic development. J Pediatr Surg. 2006;41:347-51 pubmed
Kitamoto J, Fukui A, Asashima M. Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation. Adv Space Res. 2005;35:1654-61 pubmed
Teague W, Jayanthi N, Lear P, Johnson P. Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick-quail chimera model. Pediatr Surg Int. 2005;21:138-42 pubmed
Levine E, Lee C, Kintner C, Gumbiner B. Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant. Development. 1994;120:901-9 pubmed
Choi Y, Gumbiner B. Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm. J Cell Biol. 1989;108:2449-58 pubmed
Levi G, Gumbiner B, Thiery J. The distribution of E-cadherin during Xenopus laevis development. Development. 1991;111:159-69 pubmed

product information

Internal ID :

3807

Name :

8C2

Depositor Name :

Gumbiner, B.M.

Depositor Institution :

Cell Biology, School of Medicine, University of Virginia

Date Deposited :

5/7/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

cadherin, E-

Antigen Species :

Xenopus

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Avian,Xenopus

Species Tested (legacy) :

Xenpous, other amphibia not tested

Initial Publication Pubmed ID :

2472408

Depositor Notes (Special Instructions) :

8C2 only binds the -(Ca++) conformation of E-cadherin. Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.

Collections :

CD antigens,Cell adhesion,Xenopus

Search Keywords :

Gumbiner, B.M., cadherin, E-, xenopus, MIgG1, xenopus/avian, cdh1, uvo, cdh1, cdhe, ecad, lcam, arc-1, cd324, AB_528117, monoclonal, Cell adhesion/Cluster Determinant antigens/Xenopus, Immunofluorescence/Western Blot/Immunoprecipitation/Function Blocking

Antigen Molecular Weight :

Predicted: 79 kDa (mature E-cadherin); Apparent: 140 kDa

Gene :

cdh1

Alternate Gene Name(s) :

uvo, cdh1, cdhe, ecad, lcam, arc-1, cd324

Uniprot ID :

P30944

Antibody Registry ID :

AB_528117

Immunogen :

Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.

Immunogen Sequence :

partial protein

Clonality :

Monoclonal

Myeloma Strain :

sp-2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

Extracellular domain

Epitope Map PubMed ID :

2472408

Recommended Applications :

Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

2472408 2015791 17208557 15578193 19521560 16481249 16175731

Western Blot Pubmed IDs :

2472408 15578193 7600966

Immunoprecipitation Pubmed IDs :

2472408 15578193

Function Blocking Pubmed IDs :

2472408

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 108, 2449-2458.; Organogenesis 1(2), 45-51.; Pediatr. Surg. Int. 21(3), 138-142.

more info or order :

Developmental Studies Hybridoma Bank product webpage