product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Glutamate receptor subunit, DGluR-IIA
catalog :
8B4D2 (MH2B)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8B4D2 (MH2B)
reactivity :
mouse, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
Developmental Studies Hybridoma Bank product webpage
citations: 42
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; fruit fly; 1:500; loading ...; fig 2c
Spring A, Brusich D, Frank C. C-terminal Src Kinase Gates Homeostatic Synaptic Plasticity and Regulates Fasciclin II Expression at the Drosophila Neuromuscular Junction. PLoS Genet. 2016;12:e1005886 pubmed publisher
  • western blot; mouse; 1:1000; fig 4
Chung H, Jacobs C, Huo Y, Yang J, Krumm S, Plemper R, et al. Tunable and reversible drug control of protein production via a self-excising degron. Nat Chem Biol. 2015;11:713-20 pubmed publisher
Wang M, Chen P, Wang C, Lai T, Tsai P, Cheng Y, et al. Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response. PLoS Genet. 2016;12:e1006362 pubmed publisher
Fernandes A, Uytterhoeven V, Kuenen S, Wang Y, Slabbaert J, Swerts J, et al. Reduced synaptic vesicle protein degradation at lysosomes curbs TBC1D24/sky-induced neurodegeneration. J Cell Biol. 2014;207:453-62 pubmed publisher
Gardiol A, St Johnston D. Staufen targets coracle mRNA to Drosophila neuromuscular junctions and regulates GluRIIA synaptic accumulation and bouton number. Dev Biol. 2014;392:153-67 pubmed publisher
Parkinson W, Dear M, Rushton E, Broadie K. N-glycosylation requirements in neuromuscular synaptogenesis. Development. 2013;140:4970-81 pubmed publisher
Castellanos M, Tang J, Allan D. Female-biased dimorphism underlies a female-specific role for post-embryonic Ilp7 neurons in Drosophila fertility. Development. 2013;140:3915-26 pubmed publisher
Lee J, Ueda A, Wu C. Distinct roles of Drosophila cacophony and Dmca1D Ca(2+) channels in synaptic homeostasis: genetic interactions with slowpoke Ca(2+) -activated BK channels in presynaptic excitability and postsynaptic response. Dev Neurobiol. 2014;74:1-15 pubmed publisher
Ruiz S, Ferreiro M, Menhert K, Casanova G, Olivera A, Cantera R. Rhythmic changes in synapse numbers in Drosophila melanogaster motor terminals. PLoS ONE. 2013;8:e67161 pubmed publisher
Lee H, Zhao N, Campion B, Nguyen M, Selleck S. Akt regulates glutamate receptor trafficking and postsynaptic membrane elaboration at the Drosophila neuromuscular junction. Dev Neurobiol. 2013;73:723-43 pubmed publisher
Staples J, Broadie K. The cell polarity scaffold Lethal Giant Larvae regulates synapse morphology and function. J Cell Sci. 2013;126:1992-2003 pubmed publisher
Fukui A, Inaki M, Tonoe G, Hamatani H, Homma M, Morimoto T, et al. Lola regulates glutamate receptor expression at the Drosophila neuromuscular junction. Biol Open. 2012;1:362-75 pubmed publisher
Matta S, Van Kolen K, da Cunha R, van den Bogaart G, Mandemakers W, Miskiewicz K, et al. LRRK2 controls an EndoA phosphorylation cycle in synaptic endocytosis. Neuron. 2012;75:1008-21 pubmed publisher
Kurusu M, Katsuki T, Zinn K, Suzuki E. Developmental changes in expression, subcellular distribution, and function of Drosophila N-cadherin, guided by a cell-intrinsic program during neuronal differentiation. Dev Biol. 2012;366:204-17 pubmed publisher
Chen K, Featherstone D. Pre and postsynaptic roles for Drosophila CASK. Mol Cell Neurosci. 2011;48:171-82 pubmed publisher
Ozdowski E, Gayle S, Bao H, Zhang B, SHERWOOD N. Loss of Drosophila melanogaster p21-activated kinase 3 suppresses defects in synapse structure and function caused by spastin mutations. Genetics. 2011;189:123-35 pubmed publisher
Koon A, Ashley J, Barria R, Dasgupta S, Brain R, Waddell S, et al. Autoregulatory and paracrine control of synaptic and behavioral plasticity by octopaminergic signaling. Nat Neurosci. 2011;14:190-9 pubmed publisher
Liu Z, Chen Y, Wang D, Wang S, Zhang Y. Distinct presynaptic and postsynaptic dismantling processes of Drosophila neuromuscular junctions during metamorphosis. J Neurosci. 2010;30:11624-34 pubmed publisher
Fouquet W, Owald D, Wichmann C, Mertel S, Depner H, Dyba M, et al. Maturation of active zone assembly by Drosophila Bruchpilot. J Cell Biol. 2009;186:129-45 pubmed publisher
Chen K, Augustin H, Featherstone D. Effect of ambient extracellular glutamate on Drosophila glutamate receptor trafficking and function. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2009;195:21-9 pubmed publisher
Arias A. Drosophila melanogaster and the development of biology in the 20th century. Methods Mol Biol. 2008;420:1-25 pubmed publisher
Lee J, Ueda A, Wu C. Pre- and post-synaptic mechanisms of synaptic strength homeostasis revealed by slowpoke and shaker K+ channel mutations in Drosophila. Neuroscience. 2008;154:1283-96 pubmed publisher
Schmid A, Hallermann S, Kittel R, Khorramshahi O, Frölich A, Quentin C, et al. Activity-dependent site-specific changes of glutamate receptor composition in vivo. Nat Neurosci. 2008;11:659-66 pubmed publisher
Bogdanik L, Framery B, Frölich A, Franco B, Mornet D, Bockaert J, et al. Muscle dystroglycan organizes the postsynapse and regulates presynaptic neurotransmitter release at the Drosophila neuromuscular junction. PLoS ONE. 2008;3:e2084 pubmed publisher
Long A, Kim E, Leung H, Woodruff E, An L, Doerge R, et al. Presynaptic calcium channel localization and calcium-dependent synaptic vesicle exocytosis regulated by the Fuseless protein. J Neurosci. 2008;28:3668-82 pubmed publisher
Pan L, Broadie K. Drosophila fragile X mental retardation protein and metabotropic glutamate receptor A convergently regulate the synaptic ratio of ionotropic glutamate receptor subclasses. J Neurosci. 2007;27:12378-89 pubmed
Gorczyca D, Ashley J, Speese S, Gherbesi N, Thomas U, Gundelfinger E, et al. Postsynaptic membrane addition depends on the Discs-Large-interacting t-SNARE Gtaxin. J Neurosci. 2007;27:1033-44 pubmed
Augustin H, Grosjean Y, Chen K, Sheng Q, Featherstone D. Nonvesicular release of glutamate by glial xCT transporters suppresses glutamate receptor clustering in vivo. J Neurosci. 2007;27:111-23 pubmed
Pielage J, Fetter R, Davis G. A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction. J Cell Biol. 2006;175:491-503 pubmed
Schmid A, Qin G, Wichmann C, Kittel R, Mertel S, Fouquet W, et al. Non-NMDA-type glutamate receptors are essential for maturation but not for initial assembly of synapses at Drosophila neuromuscular junctions. J Neurosci. 2006;26:11267-77 pubmed
Liebl F, Chen K, Karr J, Sheng Q, Featherstone D. Increased synaptic microtubules and altered synapse development in Drosophila sec8 mutants. BMC Biol. 2005;3:27 pubmed
Chen K, Merino C, Sigrist S, Featherstone D. The 4.1 protein coracle mediates subunit-selective anchoring of Drosophila glutamate receptors to the postsynaptic actin cytoskeleton. J Neurosci. 2005;25:6667-75 pubmed
Liebl F, Featherstone D. Genes involved in Drosophila glutamate receptor expression and localization. BMC Neurosci. 2005;6:44 pubmed
Pielage J, Fetter R, Davis G. Presynaptic spectrin is essential for synapse stabilization. Curr Biol. 2005;15:918-28 pubmed
Qin G, Schwarz T, Kittel R, Schmid A, Rasse T, Kappei D, et al. Four different subunits are essential for expressing the synaptic glutamate receptor at neuromuscular junctions of Drosophila. J Neurosci. 2005;25:3209-18 pubmed
Featherstone D, Rushton E, Rohrbough J, Liebl F, Karr J, Sheng Q, et al. An essential Drosophila glutamate receptor subunit that functions in both central neuropil and neuromuscular junction. J Neurosci. 2005;25:3199-208 pubmed
Dulcis D, Levine R. Glutamatergic innervation of the heart initiates retrograde contractions in adult Drosophila melanogaster. J Neurosci. 2005;25:271-80 pubmed
Chen K, Featherstone D. Discs-large (DLG) is clustered by presynaptic innervation and regulates postsynaptic glutamate receptor subunit composition in Drosophila. BMC Biol. 2005;3:1 pubmed
Albin S, Davis G. Coordinating structural and functional synapse development: postsynaptic p21-activated kinase independently specifies glutamate receptor abundance and postsynaptic morphology. J Neurosci. 2004;24:6871-9 pubmed
Marrus S, Portman S, Allen M, Moffat K, DiAntonio A. Differential localization of glutamate receptor subunits at the Drosophila neuromuscular junction. J Neurosci. 2004;24:1406-15 pubmed
Yoshihara M, Littleton J. Synaptotagmin I functions as a calcium sensor to synchronize neurotransmitter release. Neuron. 2002;36:897-908 pubmed
Featherstone D, Rushton E, Broadie K. Developmental regulation of glutamate receptor field size by nonvesicular glutamate release. Nat Neurosci. 2002;5:141-6 pubmed
product information
Internal ID :
3598
Name :
8B4D2 (MH2B)
Depositor Name :
Goodman, C.
Depositor Institution :
University of California, Berkeley
Date Deposited :
6/27/01
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Glutamate receptor subunit, DGluR-IIA
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG2a, kappa light chain
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila; not tested in other species
Initial Publication Pubmed ID :
22542600
Depositor Notes (Special Instructions) :
Recognizes antigen in bacterial extracts, stains larval neuromuscular junctions faintly
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Goodman, Corey, Glutamate receptor subunit, DGluR-IIA, Drosophila, MIgG2a, kappa light chain, Drosophila, GluRIIA, D-GluRIIA; DGluR-II; DGluR-IIA; DGluR2a; dGluRII; DmelGluRIIA; Glu-RII; Glu-RIIA;, AB_528269, monoclonal
Antigen Molecular Weight :
110 kDa
Gene :
GluRIIA
Alternate Gene Name(s) :
D-GluRIIA; DGluR-II; DGluR-IIA; DGluR2a; dGluRII; DmelGluRIIA; Glu-RII; Glu-RIIA;
Uniprot ID :
Q9VMP4
Antibody Registry ID :
AB_528269
Immunogen :
DGluR-IIA (a.a. 633-786)-6X-His-fusion protein.
Clonality :
Monoclonal
Myeloma Strain :
P3 x 63 Ag8.653
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Recognizes antigen in bacterial extracts
Immunohistochemistry Pubmed IDs :
23592328 20810883 23213426 22998870 24951879 23959639 21820054 23981656 24227656 23444371 21186359 15916948 18446215 17989302 18539401 18641938 17088429 15638945 17202478 18385325 19596851 11753421 15295021 15647470 15788778 17079654 15788777 17267557 16351720 16014728 18469810 14960613
Immunofluorescence Pubmed IDs :
25422373 21705760 23213426 24951879 23840613 21820054 23981656 24227656 23444371 21186359 22542600 15916948 18446215 18539401 17088429 15638945 17202478 18941757 18385325 19596851 11753421 15985179 15647470 17079654 15788777 17267557 16351720 16014728 18469810 27736876 29748610
Western Blot Pubmed IDs :
23213426 12467593 18941757 16351720
DSHB Growth Medium :
RPMI
References (legacy) :
Dev. Biol. 366, 204-217.
more info or order :
Developmental Studies Hybridoma Bank product webpage
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US