receptor-linked protein tyrosine phosphatase 10D, DPTP10D, Drosophila | Developmental Studies Hybridoma Bank 8B22F5 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

receptor-linked protein tyrosine phosphatase 10D, DPTP10D, Drosophila

catalog :

8B22F5

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8B22F5

reactivity :

fruit fly

application :

immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Reference
Tsarouhas V, Liu D, Tsikala G, Fedoseienko A, Zinn K, Matsuda R, et al. WASH phosphorylation balances endosomal versus cortical actin network integrities during epithelial morphogenesis. Nat Commun. 2019;10:2193 pubmed publisher
Lee H, Cording A, Vielmetter J, Zinn K. Interactions between a receptor tyrosine phosphatase and a cell surface ligand regulate axon guidance and glial-neuronal communication. Neuron. 2013;78:813-26 pubmed publisher
Jeon M, Scott M, Zinn K. Interactions between Type III receptor tyrosine phosphatases and growth factor receptor tyrosine kinases regulate tracheal tube formation in Drosophila. Biol Open. 2012;1:548-58 pubmed publisher
Jeon M, Zinn K. Receptor tyrosine phosphatases control tracheal tube geometries through negative regulation of Egfr signaling. Development. 2009;136:3121-9 pubmed publisher
Kurusu M, Zinn K. Receptor tyrosine phosphatases regulate birth order-dependent axonal fasciculation and midline repulsion during development of the Drosophila mushroom body. Mol Cell Neurosci. 2008;38:53-65 pubmed publisher
Jeon M, Nguyen H, Bahri S, Zinn K. Redundancy and compensation in axon guidance: genetic analysis of the Drosophila Ptp10D/Ptp4E receptor tyrosine phosphatase subfamily. Neural Dev. 2008;3:3 pubmed publisher

product information

Internal ID :

1238

Name :

8B22F5

Depositor Name :

Zinn, K.

Depositor Institution :

California Institute of Technology

Date Deposited :

6/16/05

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

receptor-linked protein tyrosine phosphatase 10D, DPTP10D, Drosophila

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

18356078

Depositor Notes (Special Instructions) :

This mAb differs from other anti-DPTP10D, as it recognizes the native form of DPTP10D and works well for IP. this mAb works much better than 45E10 (a different anti-DPTP10D clone available from DSHB) for staining larval material; the two mAbs are equivalent on embryos.

Collections :

Cell markers,Cell signaling,Drosophila antigens,Enzymes,Neurodevelopment

Search Keywords :

Zinn, K., receptor-linked protein tyrosine phosphatase 10D, DPTP10D, Drosophila, Drosophila, MIgG1, kappa light chain, Drosophila, Ptp10D, CG1817, AB_528443, monoclonal, Cell signaling/Drosophila antigens/Enzymes/Neurodevelopment/Cell markers, Immunofluorescence/Immunoprecipitation

Antigen Molecular Weight :

Predicted: multiple isoforms between 177 and 222 kDa; Apparent: 200 kDa

Gene :

Ptp10D

Alternate Gene Name(s) :

CG1817

Uniprot ID :

P35992

Antibody Registry ID :

AB_528443

Synonyms (Alternate Clone Names) :

8B2

Immunogen :

Recombinant fusion protein containing the extracellular domain of PTP10D produced in monkey COS7 cells.

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

HL-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Extracellular domain

Epitope Map PubMed ID :

18356078

Recommended Applications :

Immunofluorescence,Immunoprecipitation

Immunoblotting (legacy) :

Immunofluorescence Pubmed IDs :

19675131 18356078 23764287 23213447 18237413

Immunoprecipitation Pubmed IDs :

19675131

Additional Information :

PTP10D is a receptor tyrosine phosphatase on motor neurons. 8B22F5stains the tracheal system and the axons of the central nervous system. The specificity of 8B22F5 was tested on Ptp10D mutants by immunoprecipitation and immunohistology.

DSHB Growth Medium :

HL-1

more info or order :

Developmental Studies Hybridoma Bank product webpage