neural associated ganglioside (9-0-acetyl-ganglioside) | Developmental Studies Hybridoma Bank 8A2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

neural associated ganglioside (9-0-acetyl-ganglioside)

catalog :

8A2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8A2

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; loading ...; fig 7d	Ghosh H, Auguadri L, Battaglia S, Simone Thirouin Z, Zemoura K, Messner S, et al. Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission. Nat Commun. 2016;7:13365 pubmed publisher
immunoprecipitation; human; fig 1g western blot; human; fig 1g	Antunica Noguerol M, BudziÃ±ski M, Druker J, Gassen N, Sokn M, Senin S, et al. The activity of the glucocorticoid receptor is regulated by SUMO conjugation to FKBP51. Cell Death Differ. 2016;23:1579-91 pubmed publisher
western blot; mouse; 1:1000; fig 1	Juárez Vicente F, Luna Pelaez N, Garcia Dominguez M. The Sumo protease Senp7 is required for proper neuronal differentiation. Biochim Biophys Acta. 2016;1863:1490-8 pubmed publisher
western blot; human; fig 2	Mendes A, Grou C, Azevedo J, Pinto M. Evaluation of the activity and substrate specificity of the human SENP family of SUMO proteases. Biochim Biophys Acta. 2016;1863:139-47 pubmed publisher
western blot; human	Berndt A, Wilkinson K, Heimann M, Bishop P, Henley J. In vivo characterization of the properties of SUMO1-specific monobodies. Biochem J. 2013;456:385-95 pubmed publisher
	Young H, Black A. Adult stem cells. Anat Rec A Discov Mol Cell Evol Biol. 2004;276:75-102 pubmed
	Young H. Existence of reserve quiescent stem cells in adults, from amphibians to humans. Curr Top Microbiol Immunol. 2004;280:71-109 pubmed
	Young H, Duplaa C, Young T, Floyd J, Reeves M, Davis K, et al. Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells. Anat Rec. 2001;263:350-60 pubmed
	Finnegan S, Lemmon V, Koenig E. Monoclonal antibody 8A2-induced retraction appears to be mediated by protein phosphorylation in goldfish retinal ganglion cell axons. Dev Biol. 1993;156:230-42 pubmed
	Yamagata M, Herman J, Sanes J. Lamina-specific expression of adhesion molecules in developing chick optic tectum. J Neurosci. 1995;15:4556-71 pubmed
	Drazba J, Pierce M, Lemmon V. Studies of the developing chick retina using monoclonal antibody 8A2 that recognizes a novel set of gangliosides. Dev Biol. 1991;145:154-63 pubmed
	Sjoberg E, Manzi A, Khoo K, Dell A, Varki A. Structural and immunological characterization of O-acetylated GD2. Evidence that GD2 is an acceptor for ganglioside O-acetyltransferase in human melanoma cells. J Biol Chem. 1992;267:16200-11 pubmed

product information

Internal ID :

2976

Name :

8A2

Depositor Name :

Lemmon, V.

Depositor Institution :

University of Miami School of Medicine

Date Deposited :

2/20/91

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

neural associated ganglioside (9-0-acetyl-ganglioside)

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Chicken,Fish,Human,Mammal,Rat

Species Tested (legacy) :

binds to neurons in all species tested, from fish to mammals

Initial Publication Pubmed ID :

2019320

Depositor Notes (Special Instructions) :

8A2 binds to neurons in all vertebrate species tested, from fish to mammals. 8A2 gangliosides are a set of rare gangliosides with a base labile epitope, suggesting the presence of a 9-0-acetyl group. The 8A2 gangliosides are different from the JONES and D1.1 gangliosides. 8A2 causes growth cone collapse of goldfish retinal ganglion cell axons. 8A2 gives an intense staining of chick embryo axons in vivo and in vitro by binding to the surface of neurons, predominantly on their axons. Tissues fixed with 2% paraformaldehyde and 0.01 glutaraldehyde, work well for immunohistology.

Collections :

Cell adhesion,Cell signaling,Human,Neurodevelopment

Search Keywords :

Lemmon, V., neural associated ganglioside (9-0-acetyl-ganglioside, Chicken, MIgM, Fish/Mammal/Human/Rat/Chicken, , , AB_531867, monoclonal, Cell adhesion/Cell signaling/Human/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA/Function Blocking

Antibody Registry ID :

AB_531867

Immunogen :

Chicken optic nerve (E14)

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Recommended Applications :

ELISA,Function Blocking,Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

2019320 11500811 14594208 14699636

Immunofluorescence Pubmed IDs :

8383619 7790923

Western Blot Pubmed IDs :

2019320 1644805

ELISA Pubmed IDs :

2019320

Function Blocking Pubmed IDs :

8383619

Additional Information :

Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.

DSHB Growth Medium :

Iscove's

References (legacy) :

Dev. Biol. 145, 154-163.; Anat. Rec. 263, 350-360.; Curr. Top. Microbiol. Immunol. 280, 71-109.; Anat. Rec. Part A 276A, 75-102.

more info or order :

Developmental Studies Hybridoma Bank product webpage