Cation-independent mannose-6-phosphate receptor (IGF2R) | Developmental Studies Hybridoma Bank 86f7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Cation-independent mannose-6-phosphate receptor (IGF2R)

catalog :

86f7

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

86f7

reactivity :

mouse, cow

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 9

Reference
Laura R, Dong D, Reynolds W, Maki R. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation. PLoS ONE. 2016;11:e0149391 pubmed publisher
Almeida C, Yamada A, Tenza D, Louvard D, Raposo G, Coudrier E. Myosin 1b promotes the formation of post-Golgi carriers by regulating actin assembly and membrane remodelling at the trans-Golgi network. Nat Cell Biol. 2011;13:779-89 pubmed publisher
Cai Q, Lu L, Tian J, Zhu Y, Qiao H, Sheng Z. Snapin-regulated late endosomal transport is critical for efficient autophagy-lysosomal function in neurons. Neuron. 2010;68:73-86 pubmed publisher
Tortorella L, Schapiro F, Maxfield F. Role of an acidic cluster/dileucine motif in cation-independent mannose 6-phosphate receptor traffic. Traffic. 2007;8:402-13 pubmed
Lin S, Mallet W, Huang A, Maxfield F. Endocytosed cation-independent mannose 6-phosphate receptor traffics via the endocytic recycling compartment en route to the trans-Golgi network and a subpopulation of late endosomes. Mol Biol Cell. 2004;15:721-33 pubmed
Wick D, Seetharam B, Dahms N. Basolateral sorting signal of the 300-kDa mannose 6-phosphate receptor. Am J Physiol Gastrointest Liver Physiol. 2002;282:G51-60 pubmed
Chen H, Remmler J, Delaney J, Messner D, Lobel P. Mutational analysis of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor. A consensus casein kinase II site followed by 2 leucines near the carboxyl terminus is important for intracellular targeting of lysosomal enzyme. J Biol Chem. 1993;268:22338-46 pubmed
Messner D. The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver. Arch Biochem Biophys. 1993;306:391-401 pubmed
Beck K, Buchanan J, Malhotra V, Nelson W. Golgi spectrin: identification of an erythroid beta-spectrin homolog associated with the Golgi complex. J Cell Biol. 1994;127:707-23 pubmed

product information

Internal ID :

3445

Name :

86f7

Depositor Name :

Messner, D.

Depositor Institution :

Bastyr University

Date Deposited :

10/21/04

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Cation-independent mannose-6-phosphate receptor (IGF2R)

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Bovine,Mouse

Species Tested (legacy) :

mouse, bovine, not human

Initial Publication Pubmed ID :

8215441

Depositor Notes (Special Instructions) :

86f7 doesn't recognize the human IGF2R. Use non-reducing conditions for western blotting.

Collections :

CD antigens,Cell signaling

Search Keywords :

Messner, D., Cation-independent mannose-6-phosphate receptor (IGF2R), Bovine, MIgG1, kappa light chain, Bovine/Mouse, IGF2R, CD222, CI-MPR, M6PR, MPR 300, IGF-IIR, AB_528348, monoclonal, Cell signaling/Cluster Determinant antigens, Immunofluorescence/Western Blot/Immunoprecipitation/ELISA

Antigen Molecular Weight :

Predicted: 270 kDa; Apparent: 300 kDa

Gene :

IGF2R

Alternate Gene Name(s) :

CD222, CI-MPR, M6PR, MPR 300, IGF-IIR

Uniprot ID :

P08169

Antibody Registry ID :

AB_528348

Synonyms (Alternate Clone Names) :

DM86f7

Immunogen :

Mannose 6-phosphate receptors mix purified from bovine calf liver.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3-X63-Ag8.653.3

Epitope Mapped :

Yes

Epitope Location or Sequence :

Extracellular region

Epitope Map PubMed ID :

8226743

Recommended Applications :

ELISA,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

8215441 11751157 14595110 20920792 26890638 7962054 17319895 21666684

Western Blot Pubmed IDs :

8215441 11751157 20920792

Immunoprecipitation Pubmed IDs :

8215441 11751157

ELISA Pubmed IDs :

8226743

DSHB Growth Medium :

RPMI

References (legacy) :

Arch. Biochem. Biophys. 306(2), 391-401.; J. Biol. Chem. 268(30), 22338-22346.; Am. J. Physiol. Gastrointest. Liver Physiol. 282, G51-G60.; Mol. Biol. Cell 15, 721-733.; Neuron 68, 73-86.

more info or order :

Developmental Studies Hybridoma Bank product webpage