product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
MNR2/HB9/Mnx1
catalog :
81.5C10
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
81.5C10
reactivity :
African green monkey, little skate, human, mouse, chicken, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
citations: 68
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:80; loading ...; fig s1
Birger A, Ottolenghi M, Perez L, Reubinoff B, Behar O. ALS-related human cortical and motor neurons survival is differentially affected by Sema3A. Cell Death Dis. 2018;9:256 pubmed publisher
  • immunohistochemistry - frozen section; little skate; fig s2c
Jung H, Baek M, D Elia K, Boisvert C, Currie P, Tay B, et al. The Ancient Origins of Neural Substrates for Land Walking. Cell. 2018;172:667-682.e15 pubmed publisher
  • immunocytochemistry; African green monkey; 1:50; loading ...; fig S5
Yamashita T, Miyamoto Y, Bando Y, Ono T, Kobayashi S, Doi A, et al. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells. PLoS ONE. 2017;12:e0171947 pubmed publisher
  • immunohistochemistry; mouse; fig s1
Airik R, Schueler M, Airik M, Cho J, Ulanowicz K, Porath J, et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS ONE. 2016;11:e0156081 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; tbl 2
Cutts J, Brookhouser N, Brafman D. Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs). Methods Mol Biol. 2016;1516:121-144 pubmed publisher
  • immunocytochemistry; mouse; fig 7
Lee B, Lee S, Agulnick A, Lee J, Lee S. Single-stranded DNA binding proteins are required for LIM complexes to induce transcriptionally active chromatin and specify spinal neuronal identities. Development. 2016;143:1721-31 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
  • immunocytochemistry; mouse; 1:20; loading ...; fig 3e
Iyer N, Huettner J, Butts J, Brown C, Sakiyama Elbert S. Generation of highly enriched V2a interneurons from mouse embryonic stem cells. Exp Neurol. 2016;277:305-316 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 5
Pellett S, Schwartz M, Tepp W, Josephson R, Scherf J, Pier C, et al. Human Induced Pluripotent Stem Cell Derived Neuronal Cells Cultured on Chemically-Defined Hydrogels for Sensitive In Vitro Detection of Botulinum Neurotoxin. Sci Rep. 2015;5:14566 pubmed publisher
  • immunohistochemistry; chicken; fig 2
Thiebes K, Nam H, Cambronne X, Shen R, Glasgow S, Cho H, et al. miR-218 is essential to establish motor neuron fate as a downstream effector of Isl1-Lhx3. Nat Commun. 2015;6:7718 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 2
Lenzi J, De Santis R, de Turris V, Morlando M, Laneve P, Calvo A, et al. ALS mutant FUS proteins are recruited into stress granules in induced pluripotent stem cell-derived motoneurons. Dis Model Mech. 2015;8:755-66 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 2
Yan Y, Wladyka C, Fujii J, Sockanathan S. Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2. Nat Commun. 2015;6:7006 pubmed publisher
  • immunohistochemistry; mouse; fig s5
Eisner A, Pazyra Murphy M, Durresi E, Zhou P, Zhao X, Chadwick E, et al. The Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis. Dev Cell. 2015;33:22-35 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 3
Maury Y, Côme J, Piskorowski R, Salah Mohellibi N, Chevaleyre V, Peschanski M, et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol. 2015;33:89-96 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 6g
Zou M, Luo H, Xiang M. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn. 2015;244:86-97 pubmed publisher
  • western blot; human; 1:100
Jha B, Rao M, Malik N. Motor neuron differentiation from pluripotent stem cells and other intermediate proliferative precursors that can be discriminated by lineage specific reporters. Stem Cell Rev. 2015;11:194-204 pubmed publisher
Kim B, Jeong Y, Wong M, Martin L. DNA damage accumulates and responses are engaged in human ALS brain and spinal motor neurons and DNA repair is activatable in iPSC-derived motor neurons with SOD1 mutations. Acta Neuropathol Commun. 2020;8:7 pubmed publisher
Lin C, Yoshida M, Li L, Ikenaka A, Oshima S, Nakagawa K, et al. iPSC-derived functional human neuromuscular junctions model the pathophysiology of neuromuscular diseases. JCI Insight. 2019;4: pubmed publisher
Nam H, Jeon S, An H, Yoo J, Lee H, Lee S, et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification. elife. 2019;8: pubmed publisher
Jacquier A, Risson V, Schaeffer L. Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons. J Vis Exp. 2019;: pubmed publisher
Yen Y, Hsieh W, Tsai Y, Lü Y, Liau E, Hsu H, et al. Dlk1-Dio3 locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identity. elife. 2018;7: pubmed publisher
Verrier L, Davidson L, Gierlinski M, Dady A, Storey K. Neural differentiation, selection and transcriptomic profiling of human neuromesodermal progenitor-like cells in vitro. Development. 2018;145: pubmed publisher
Hsu F, Spannl S, Ferguson C, Hyman A, Parton R, Zerial M. Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria. elife. 2018;7: pubmed publisher
Wang C, Li J, Meng Q, Wang B. Three Tctn proteins are functionally conserved in the regulation of neural tube patterning and Gli3 processing but not ciliogenesis and Hedgehog signaling in the mouse. Dev Biol. 2017;430:156-165 pubmed publisher
Laumonnerie C, Tong Y, Alstermark H, Wilson S. Commissural axonal corridors instruct neuronal migration in the mouse spinal cord. Nat Commun. 2015;6:7028 pubmed publisher
Johnson Kerner B, Ahmad F, Diaz A, Greene J, Gray S, Samulski R, et al. Intermediate filament protein accumulation in motor neurons derived from giant axonal neuropathy iPSCs rescued by restoration of gigaxonin. Hum Mol Genet. 2015;24:1420-31 pubmed publisher
Francius C, Ravassard P, Hidalgo Figueroa M, Mallet J, Clotman F, Nardelli J. Genetic dissection of Gata2 selective functions during specification of V2 interneurons in the developing spinal cord. Dev Neurobiol. 2015;75:721-37 pubmed publisher
Lamas N, Johnson Kerner B, Roybon L, Kim Y, Garcia Diaz A, Wichterle H, et al. Neurotrophic requirements of human motor neurons defined using amplified and purified stem cell-derived cultures. PLoS ONE. 2014;9:e110324 pubmed publisher
Maeda M, Harris A, Kingham B, Lumpkin C, Opdenaker L, McCahan S, et al. Transcriptome profiling of spinal muscular atrophy motor neurons derived from mouse embryonic stem cells. PLoS ONE. 2014;9:e106818 pubmed publisher
Lin J, Wang C, Redies C. Restricted expression of classic cadherins in the spinal cord of the chicken embryo. Front Neuroanat. 2014;8:18 pubmed publisher
Yan X, Lin J, Talabattula V, Mußmann C, Yang F, Wree A, et al. ADAM10 negatively regulates neuronal differentiation during spinal cord development. PLoS ONE. 2014;9:e84617 pubmed publisher
Park S, Mullen R, Rhodes S. Cell-specific actions of a human LHX3 gene enhancer during pituitary and spinal cord development. Mol Endocrinol. 2013;27:2013-27 pubmed publisher
Ricard M, Gudas L. Cytochrome p450 cyp26a1 alters spinal motor neuron subtype identity in differentiating embryonic stem cells. J Biol Chem. 2013;288:28801-13 pubmed publisher
Su H, Wang L, Cai J, Yuan Q, Yang X, Yao X, et al. Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle. Stem Cell Res. 2013;11:529-39 pubmed publisher
Kobayashi N, Homma S, Okada T, Masuda T, Sato N, Nishiyama K, et al. Elucidation of target muscle and detailed development of dorsal motor neurons in chick embryo spinal cord. J Comp Neurol. 2013;521:2987-3002 pubmed publisher
Kim K, Nam J, Mukouyama Y, Kawamoto S. Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development. J Cell Biol. 2013;200:443-58 pubmed publisher
Hunter C, Stein R. Characterization of an apparently novel ?-cell line-enriched 80-88 kDa transcriptional activator of the MafA and Pdx1 genes. J Biol Chem. 2013;288:3795-803 pubmed publisher
Grumbles R, Almeida V, Casella G, Wood P, Hemstapat K, Thomas C. Motoneuron replacement for reinnervation of skeletal muscle in adult rats. J Neuropathol Exp Neurol. 2012;71:921-30 pubmed
Roy A, Francius C, Rousso D, Seuntjens E, Debruyn J, Luxenhofer G, et al. Onecut transcription factors act upstream of Isl1 to regulate spinal motoneuron diversification. Development. 2012;139:3109-19 pubmed publisher
Takazawa T, Croft G, Amoroso M, Studer L, Wichterle H, Macdermott A. Maturation of spinal motor neurons derived from human embryonic stem cells. PLoS ONE. 2012;7:e40154 pubmed publisher
Estarás C, Akizu N, Garcia A, Beltran S, de la Cruz X, Martínez Balbás M. Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Development. 2012;139:2681-91 pubmed publisher
Imsland F, Feng C, Boije H, Bed Hom B, Fillon V, Dorshorst B, et al. The Rose-comb mutation in chickens constitutes a structural rearrangement causing both altered comb morphology and defective sperm motility. PLoS Genet. 2012;8:e1002775 pubmed publisher
Han L, Pan Y, Wang B. Small ubiquitin-like Modifier (SUMO) modification inhibits GLI2 protein transcriptional activity in vitro and in vivo. J Biol Chem. 2012;287:20483-9 pubmed publisher
Kuscha V, Frazer S, Dias T, Hibi M, Becker T, Becker C. Lesion-induced generation of interneuron cell types in specific dorsoventral domains in the spinal cord of adult zebrafish. J Comp Neurol. 2012;520:3604-16 pubmed publisher
Zhong Z, Ohnmacht J, Reimer M, Bach I, Becker T, Becker C. Chondrolectin mediates growth cone interactions of motor axons with an intermediate target. J Neurosci. 2012;32:4426-39 pubmed publisher
Halasi G, Søviknes A, Sigurjonsson O, Glover J. Proliferation and recapitulation of developmental patterning associated with regulative regeneration of the spinal cord neural tube. Dev Biol. 2012;365:118-32 pubmed publisher
Todd K, Lan Chow Wing N, Salin Cantegrel A, Cotter A, Zagami C, Lo R, et al. Establishment of motor neuron-V3 interneuron progenitor domain boundary in ventral spinal cord requires Groucho-mediated transcriptional corepression. PLoS ONE. 2012;7:e31176 pubmed publisher
Usui N, Watanabe K, Ono K, Tomita K, Tamamaki N, Ikenaka K, et al. Role of motoneuron-derived neurotrophin 3 in survival and axonal projection of sensory neurons during neural circuit formation. Development. 2012;139:1125-32 pubmed publisher
Lin J, Wang C, Redies C. Expression of delta-protocadherins in the spinal cord of the chicken embryo. J Comp Neurol. 2012;520:1509-31 pubmed publisher
Mich J, Chen J. Hedgehog and retinoic acid signaling cooperate to promote motoneurogenesis in zebrafish. Development. 2011;138:5113-9 pubmed publisher
Tuson M, He M, Anderson K. Protein kinase A acts at the basal body of the primary cilium to prevent Gli2 activation and ventralization of the mouse neural tube. Development. 2011;138:4921-30 pubmed publisher
Berbari N, Kin N, Sharma N, Michaud E, Kesterson R, Yoder B. Mutations in Traf3ip1 reveal defects in ciliogenesis, embryonic development, and altered cell size regulation. Dev Biol. 2011;360:66-76 pubmed publisher
Itoh A, Horiuchi M, Wakayama K, Xu J, Bannerman P, Pleasure D, et al. ZPK/DLK, a mitogen-activated protein kinase kinase kinase, is a critical mediator of programmed cell death of motoneurons. J Neurosci. 2011;31:7223-8 pubmed publisher
Ocbina P, Eggenschwiler J, Moskowitz I, Anderson K. Complex interactions between genes controlling trafficking in primary cilia. Nat Genet. 2011;43:547-53 pubmed publisher
Patani R, Hollins A, Wishart T, Puddifoot C, Alvarez S, de Lera A, et al. Retinoid-independent motor neurogenesis from human embryonic stem cells reveals a medial columnar ground state. Nat Commun. 2011;2:214 pubmed publisher
Zhang Y, Wang J, Chen G, Fan D, Deng M. Inhibition of Sirt1 promotes neural progenitors toward motoneuron differentiation from human embryonic stem cells. Biochem Biophys Res Commun. 2011;404:610-4 pubmed publisher
Hashemi H, Hurley M, Gibson A, Panova V, Tchetchelnitski V, Barr A, et al. Receptor tyrosine phosphatase PTP? is a regulator of spinal cord neurogenesis. Mol Cell Neurosci. 2011;46:469-82 pubmed publisher
Holz A, Kollmus H, Ryge J, Niederkofler V, Dias J, Ericson J, et al. The transcription factors Nkx2.2 and Nkx2.9 play a novel role in floor plate development and commissural axon guidance. Development. 2010;137:4249-60 pubmed publisher
Hedlund E, Karlsson M, Osborn T, Ludwig W, Isacson O. Global gene expression profiling of somatic motor neuron populations with different vulnerability identify molecules and pathways of degeneration and protection. Brain. 2010;133:2313-30 pubmed publisher
Asli N, Kessel M. Spatiotemporally restricted regulation of generic motor neuron programs by miR-196-mediated repression of Hoxb8. Dev Biol. 2010;344:857-68 pubmed publisher
Periz G, Yan Y, Bitzer Z, Sockanathan S. GDP-bound Galphai2 regulates spinal motor neuron differentiation through interaction with GDE2. Dev Biol. 2010;341:213-21 pubmed publisher
Francius C, Clotman F. Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development. Neuroscience. 2010;165:116-29 pubmed publisher
Hu B, Zhang S. Differentiation of spinal motor neurons from pluripotent human stem cells. Nat Protoc. 2009;4:1295-304 pubmed publisher
Cameron D, Pennimpede T, Petkovich M. Tulp3 is a critical repressor of mouse hedgehog signaling. Dev Dyn. 2009;238:1140-9 pubmed publisher
Cao X, Pfaff S, Gage F. A functional study of miR-124 in the developing neural tube. Genes Dev. 2007;21:531-6 pubmed
Liu Y, Helms A, Johnson J. Distinct activities of Msx1 and Msx3 in dorsal neural tube development. Development. 2004;131:1017-28 pubmed
Thaler J, Lee S, Jurata L, Gill G, Pfaff S. LIM factor Lhx3 contributes to the specification of motor neuron and interneuron identity through cell-type-specific protein-protein interactions. Cell. 2002;110:237-49 pubmed
Takebayashi H, Nabeshima Y, Yoshida S, Chisaka O, Ikenaka K, Nabeshima Y. The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages. Curr Biol. 2002;12:1157-63 pubmed
Tanabe Y, William C, Jessell T. Specification of motor neuron identity by the MNR2 homeodomain protein. Cell. 1998;95:67-80 pubmed
product information
Internal ID :
2446
Name :
81.5C10
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
HHMI/Columbia University
Date Deposited :
2/12/99
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
MNR2/HB9/Mnx1
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Fish,Human,Mouse,Zebrafish
Species Tested (legacy) :
zebrafish, fish, human
Initial Publication Pubmed ID :
9778248
Collections :
Human,Neurodevelopment,Transcription factors
Search Keywords :
Jessell, Thomas M / Brenner-Morton, Susan, MNR2/HB9/Mnx1, Chicken, MIgG1, kappa light chain, Rodent/Chicken/Human/Fish/Rat, MNR2, , AB_2145209, monoclonal
Gene :
MNR2
Uniprot ID :
Q9YHY8
Antibody Registry ID :
AB_2145209
Immunogen :
MNR2 NH2(C-terminus; chick)-GST fusion protein expressed in E. coli.
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunoblotting (legacy) :
no
Immunohistochemistry Pubmed IDs :
9778248 24404179 12121626 25369423 12150931 14973289 17344415 19334287 22318233 22549777 24744704 25398950 25179941 20197066 21593306 22457492 23578695 21068056 22782721 22007132 22069185 23504940 22102158 21945076 21112398 21552265 24100213 20553899 22761584 22473852 23420872 22833130 22363571 22370002
Immunofluorescence Pubmed IDs :
9778248 21144831 24404179 25191843 12121626 14973289 19334287 22549777 24744704 25337699 25383599 25179941 20197066 21593306 22457492 23578695 20826431 22782721 22069185 23946489 19800948 23504940 22102158 21945076 21112398 21552265 24100213 20553899 22964786 22761584 22473852 23420872 22833130 22363571 19696748 25960414 28800946 27106497 30311912
Gel Supershift Pubmed IDs :
23269676
Additional Information :
recognize Mnx1 (mouse, PMID: 25179941)
DSHB Growth Medium :
Iscove's
References (legacy) :
Cell 95, 67-80.; Curr. Biol. 12, 1157-1163.; Cell 110, 237-249.; Development 131, 1017-1028.; Genes Dev. 21, 531-536.; Dev. Dyn. 238, 1140-1149.; Biochem. Biophys. Res. Commun. 404, 610-614.; Development 139, 1125-1132.; J. Biol. Chem. 287(24), 20483-20489.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US