product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Podoplanin
catalog :
8.1.1
clonality :
monoclonal
host :
Syrian golden hamster
conjugate :
nonconjugated
clone name :
8.1.1
reactivity :
mouse, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 66
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:500; fig s2c
Wutschka J, Kast B, Sator Schmitt M, Appak Baskoy S, Hess J, Sinn H, et al. JUNB suppresses distant metastasis by influencing the initial metastatic stage. Clin Exp Metastasis. 2021;38:411-423 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 3f
Liberti D, Kremp M, Liberti W, Penkala I, Li S, Zhou S, et al. Alveolar epithelial cell fate is maintained in a spatially restricted manner to promote lung regeneration after acute injury. Cell Rep. 2021;35:109092 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 1s4b
Zhang K, Yao E, Lin C, Chou Y, Wong J, Li J, et al. A mammalian Wnt5a-Ror2-Vangl2 axis controls the cytoskeleton and confers cellular properties required for alveologenesis. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1f
Ikonomou L, Herriges M, Lewandowski S, Marsland R, Villacorta Martin C, Caballero I, et al. The in vivo genetic program of murine primordial lung epithelial progenitors. Nat Commun. 2020;11:635 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:50; loading ...; fig 2a
Zmojdzian M, de Joussineau S, Da Ponte J, Jagla K. Distinct subsets of Eve-positive pericardial cells stabilise cardiac outflow and contribute to Hox gene-triggered heart morphogenesis in Drosophila. Development. 2018;145: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:20; loading ...; fig 2a
Guo H, Kazadaeva Y, Ortega F, Manjunath N, Desai T. Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung. Dev Biol. 2017;430:214-223 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2d
Lin C, Yao E, Zhang K, Jiang X, Croll S, Thompson Peer K, et al. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis. elife. 2017;6: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4b
Weeden C, Chen Y, Ma S, Hu Y, Ramm G, Sutherland K, et al. Lung Basal Stem Cells Rapidly Repair DNA Damage Using the Error-Prone Nonhomologous End-Joining Pathway. PLoS Biol. 2017;15:e2000731 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1h
Kechele D, Blue R, Zwarycz B, Espenschied S, Mah A, Siegel M, et al. Orphan Gpr182 suppresses ERK-mediated intestinal proliferation during regeneration and adenoma formation. J Clin Invest. 2017;127:593-607 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig e1d
Nonomura K, Woo S, Chang R, Gillich A, Qiu Z, Francisco A, et al. Piezo2 senses airway stretch and mediates lung inflation-induced apnoea. Nature. 2017;541:176-181 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 4
Laresgoiti U, Nikolić M, Rao C, Brady J, Richardson R, Batchen E, et al. Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate. Development. 2016;143:3686-3699 pubmed
  • immunohistochemistry - frozen section; mouse; fig 1d
Karaman S, Hollmén M, Yoon S, Alkan H, Alitalo K, Wolfrum C, et al. Transgenic overexpression of VEGF-C induces weight gain and insulin resistance in mice. Sci Rep. 2016;6:31566 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s7c
Crosswhite P, Podsiadlowska J, Curtis C, Gao S, Xia L, Srinivasan R, et al. CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity. J Clin Invest. 2016;126:2254-66 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3aa
San Agustin J, Klena N, Granath K, Panigrahy A, Stewart E, Devine W, et al. Genetic link between renal birth defects and congenital heart disease. Nat Commun. 2016;7:11103 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 3
Gao L, Jiang Y, Mu L, Liu Y, Wang F, Wang P, et al. Efficient Generation of Mice with Consistent Transgene Expression by FEEST. Sci Rep. 2015;5:16284 pubmed publisher
  • flow cytometry; mouse; fig 1g
Sun L, Sun C, Liang Z, Li H, Chen L, Luo H, et al. FSP1(+) fibroblast subpopulation is essential for the maintenance and regeneration of medullary thymic epithelial cells. Sci Rep. 2015;5:14871 pubmed publisher
  • flow cytometry; mouse; 1:500
Rouhani S, Eccles J, Riccardi P, Peske J, Tewalt E, Cohen J, et al. Roles of lymphatic endothelial cells expressing peripheral tissue antigens in CD4 T-cell tolerance induction. Nat Commun. 2015;6:6771 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 5 ug/ml; fig s7
Ngo J, Matsuyama M, Kim C, Poventud Fuentes I, Bates A, Siedlak S, et al. Bax deficiency extends the survival of Ku70 knockout mice that develop lung and heart diseases. Cell Death Dis. 2015;6:e1706 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:250; fig 6
Plosa E, Young L, Gulleman P, Polosukhin V, Zaynagetdinov R, Benjamin J, et al. Epithelial β1 integrin is required for lung branching morphogenesis and alveolarization. Development. 2014;141:4751-62 pubmed publisher
Katsura H, Kobayashi Y, Tata P, Hogan B. IL-1 and TNFα Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration. Stem Cell Reports. 2019;12:657-666 pubmed publisher
Frye M, Taddei A, Dierkes C, Martínez Corral I, Fielden M, Ortsäter H, et al. Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program. Nat Commun. 2018;9:1511 pubmed publisher
Turner V, Mabbott N. Structural and functional changes to lymph nodes in ageing mice. Immunology. 2017;151:239-247 pubmed publisher
Liang J, Zhang Y, Xie T, Liu N, Chen H, Geng Y, et al. Hyaluronan and TLR4 promote surfactant-protein-C-positive alveolar progenitor cell renewal and prevent severe pulmonary fibrosis in mice. Nat Med. 2016;22:1285-1293 pubmed publisher
Clark J, O keefe A, Mastracci T, Sussel L, Matise M, Kucenas S. Mammalian Nkx2.2+ perineurial glia are essential for motor nerve development. Dev Dyn. 2014;243:1116-29 pubmed publisher
Guo Y, Mishra A, Weng T, Chintagari N, Wang Y, Zhao C, et al. Wnt3a mitigates acute lung injury by reducing P2X7 receptor-mediated alveolar epithelial type I cell death. Cell Death Dis. 2014;5:e1286 pubmed publisher
Alanis D, Chang D, Akiyama H, Krasnow M, Chen J. Two nested developmental waves demarcate a compartment boundary in the mouse lung. Nat Commun. 2014;5:3923 pubmed publisher
Blum K, Karaman S, Proulx S, Ochsenbein A, Luciani P, Leroux J, et al. Chronic high-fat diet impairs collecting lymphatic vessel function in mice. PLoS ONE. 2014;9:e94713 pubmed publisher
Zgraggen S, Huggenberger R, Kerl K, Detmar M. An important role of the SDF-1/CXCR4 axis in chronic skin inflammation. PLoS ONE. 2014;9:e93665 pubmed publisher
Turcatel G, Rubin N, Menke D, Martin G, Shi W, Warburton D. Lung mesenchymal expression of Sox9 plays a critical role in tracheal development. BMC Biol. 2013;11:117 pubmed publisher
Johnson L, Jackson D. The chemokine CX3CL1 promotes trafficking of dendritic cells through inflamed lymphatics. J Cell Sci. 2013;126:5259-70 pubmed publisher
Herzog B, Fu J, Wilson S, Hess P, Sen A, McDaniel J, et al. Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2. Nature. 2013;502:105-9 pubmed publisher
Iolyeva M, Aebischer D, Proulx S, Willrodt A, Ecoiffier T, Häner S, et al. Interleukin-7 is produced by afferent lymphatic vessels and supports lymphatic drainage. Blood. 2013;122:2271-81 pubmed publisher
Majima T, Takeuchi K, Sano K, Hirashima M, Zankov D, Tanaka Okamoto M, et al. An Adaptor Molecule Afadin Regulates Lymphangiogenesis by Modulating RhoA Activity in the Developing Mouse Embryo. PLoS ONE. 2013;8:e68134 pubmed publisher
Figueira R, Watson K, Holden D, Helaine S. Identification of salmonella pathogenicity island-2 type III secretion system effectors involved in intramacrophage replication of S. enterica serovar typhimurium: implications for rational vaccine design. MBio. 2013;4:e00065 pubmed publisher
Takekoshi T, Fang L, Paragh G, Hwang S. CCR7-expressing B16 melanoma cells downregulate interferon-?-mediated inflammation and increase lymphangiogenesis in the tumor microenvironment. Oncogenesis. 2012;1:e9 pubmed publisher
Krishnan H, Ochoa Alvarez J, Shen Y, Nevel E, Lakshminarayanan M, Williams M, et al. Serines in the intracellular tail of podoplanin (PDPN) regulate cell motility. J Biol Chem. 2013;288:12215-21 pubmed publisher
Nechiporuk T, Klezovitch O, Nguyen L, Vasioukhin V. Dlg5 maintains apical aPKC and regulates progenitor differentiation during lung morphogenesis. Dev Biol. 2013;377:375-84 pubmed publisher
Iolyeva M, Karaman S, Willrodt A, Weingartner S, Vigl B, Halin C. Novel role for ALCAM in lymphatic network formation and function. FASEB J. 2013;27:978-90 pubmed publisher
Betterman K, Paquet Fifield S, Asselin Labat M, Visvader J, Butler L, Stacker S, et al. Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli. Am J Pathol. 2012;181:2225-38 pubmed publisher
Arora R, Metzger R, Papaioannou V. Multiple roles and interactions of Tbx4 and Tbx5 in development of the respiratory system. PLoS Genet. 2012;8:e1002866 pubmed publisher
Ochoa Alvarez J, Krishnan H, Shen Y, Acharya N, Han M, McNulty D, et al. Plant lectin can target receptors containing sialic acid, exemplified by podoplanin, to inhibit transformed cell growth and migration. PLoS ONE. 2012;7:e41845 pubmed publisher
Larrieu Lahargue F, Welm A, Bouchecareilh M, Alitalo K, Li D, Bikfalvi A, et al. Blocking Fibroblast Growth Factor receptor signaling inhibits tumor growth, lymphangiogenesis, and metastasis. PLoS ONE. 2012;7:e39540 pubmed publisher
Fletcher A, Malhotra D, Acton S, Lukacs Kornek V, Bellemare Pelletier A, Curry M, et al. Reproducible isolation of lymph node stromal cells reveals site-dependent differences in fibroblastic reticular cells. Front Immunol. 2011;2:35 pubmed publisher
Tan K, Yeo K, Wong F, Lim H, Khoo K, Abastado J, et al. Expansion of cortical and medullary sinuses restrains lymph node hypertrophy during prolonged inflammation. J Immunol. 2012;188:4065-80 pubmed publisher
Hashimoto S, Chen H, Que J, Brockway B, Drake J, Snyder J, et al. ?-Catenin-SOX2 signaling regulates the fate of developing airway epithelium. J Cell Sci. 2012;125:932-42 pubmed publisher
Rosser J, Bonewald L. Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. Methods Mol Biol. 2012;816:67-81 pubmed publisher
Rygiel T, Karnam G, Goverse G, van der Marel A, Greuter M, van Schaarenburg R, et al. CD200-CD200R signaling suppresses anti-tumor responses independently of CD200 expression on the tumor. Oncogene. 2012;31:2979-88 pubmed publisher
Porter J, Bukey B, Geyer A, Willnauer C, Reynolds P. Immunohistochemical detection and regulation of ?5 nicotinic acetylcholine receptor (nAChR) subunits by FoxA2 during mouse lung organogenesis. Respir Res. 2011;12:82 pubmed publisher
Habermehl D, Parkitna J, Kaden S, Brugger B, Wieland F, Grone H, et al. Glucocorticoid activity during lung maturation is essential in mesenchymal and less in alveolar epithelial cells. Mol Endocrinol. 2011;25:1280-8 pubmed publisher
Rock J, Gao X, Xue Y, Randell S, Kong Y, Hogan B. Notch-dependent differentiation of adult airway basal stem cells. Cell Stem Cell. 2011;8:639-48 pubmed publisher
Vigl B, Aebischer D, Nitschké M, Iolyeva M, Röthlin T, Antsiferova O, et al. Tissue inflammation modulates gene expression of lymphatic endothelial cells and dendritic cell migration in a stimulus-dependent manner. Blood. 2011;118:205-15 pubmed publisher
Lv H, Havari E, Pinto S, Gottumukkala R, Cornivelli L, Raddassi K, et al. Impaired thymic tolerance to ?-myosin directs autoimmunity to the heart in mice and humans. J Clin Invest. 2011;121:1561-73 pubmed publisher
Link A, Hardie D, Favre S, Britschgi M, Adams D, Sixt M, et al. Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans. Am J Pathol. 2011;178:1662-75 pubmed publisher
De Paepe M, Mao Q, Ghanta S, Hovanesian V, Padbury J. Alveolar epithelial cell therapy with human cord blood-derived hematopoietic progenitor cells. Am J Pathol. 2011;178:1329-39 pubmed publisher
Tompkins D, Besnard V, Lange A, Keiser A, Wert S, Bruno M, et al. Sox2 activates cell proliferation and differentiation in the respiratory epithelium. Am J Respir Cell Mol Biol. 2011;45:101-10 pubmed publisher
Shalapour S, Deiser K, Sercan O, Tuckermann J, Minnich K, Willimsky G, et al. Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo. Eur J Immunol. 2010;40:2391-400 pubmed publisher
Jurisic G, Iolyeva M, Proulx S, Halin C, Detmar M. Thymus cell antigen 1 (Thy1, CD90) is expressed by lymphatic vessels and mediates cell adhesion to lymphatic endothelium. Exp Cell Res. 2010;316:2982-92 pubmed publisher
Pedrioli D, Karpanen T, Dabouras V, Jurisic G, van de Hoek G, Shin J, et al. miR-31 functions as a negative regulator of lymphatic vascular lineage-specific differentiation in vitro and vascular development in vivo. Mol Cell Biol. 2010;30:3620-34 pubmed publisher
Larrieu Lahargue F, Welm A, Thomas K, Li D. Netrin-4 induces lymphangiogenesis in vivo. Blood. 2010;115:5418-26 pubmed publisher
Cohen J, Guidi C, Tewalt E, Qiao H, Rouhani S, Ruddell A, et al. Lymph node-resident lymphatic endothelial cells mediate peripheral tolerance via Aire-independent direct antigen presentation. J Exp Med. 2010;207:681-8 pubmed publisher
Fletcher A, Lukacs Kornek V, Reynoso E, Pinner S, Bellemare Pelletier A, Curry M, et al. Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions. J Exp Med. 2010;207:689-97 pubmed publisher
Tzeng T, Chyou S, Tian S, Webster B, Carpenter A, Guaiquil V, et al. CD11c(hi) dendritic cells regulate the re-establishment of vascular quiescence and stabilization after immune stimulation of lymph nodes. J Immunol. 2010;184:4247-57 pubmed publisher
Koo B, Coe D, Dixon L, Somerville R, Nelson C, Wang L, et al. ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells. Am J Pathol. 2010;176:1494-504 pubmed publisher
Johnson L, Clasper S, Holt A, Lalor P, Baban D, Jackson D. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J Exp Med. 2006;203:2763-77 pubmed
Farr A, Berry M, Kim A, Nelson A, Welch M, Aruffo A. Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues. J Exp Med. 1992;176:1477-82 pubmed
Farr A, Nelson A, Hosier S. Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus. J Histochem Cytochem. 1992;40:651-64 pubmed
product information
Internal ID :
3025
Name :
8.1.1
Depositor Name :
Farr, A.G .
Depositor Institution :
University of Washington
Date Deposited :
6/7/01
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Podoplanin
Antigen Species :
Mouse
Host Species :
Syrian hamster
Isotype :
Syrian Hamster IgG
Isotype for catalog (legacy) :
IgG
Positive Tested Species Reactivity :
Mouse
Species Tested (legacy) :
mouse-other species not tested
Initial Publication Pubmed ID :
1402691
Depositor Notes (Special Instructions) :
Reacts with T1 alpha in the lung, podoplanin in the kidney, and lymphatic endothelium; Recognizes aggrus/podoplanin expressed by platelets; Stains formalin-fixed paraffin embedded samples.
Collections :
CD antigens,Cell markers
Search Keywords :
Farr, Andrew G, Podoplanin, Mouse, Syrian Hamster IgG, Mouse, Pdpn, Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha, AB_531893, monoclonal
Antigen Molecular Weight :
38 kDa (36-40 kDa)
Gene :
Pdpn
Alternate Gene Name(s) :
Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
Uniprot ID :
Q62011
Antibody Registry ID :
AB_531893
Synonyms (Alternate Clone Names) :
glycoprotein, 38 kDa, mucin-like
Immunogen :
Thymic stromal cell line E-71 (murine).
Clonality :
Monoclonal
Myeloma Strain :
P3x
Epitope Mapped :
No
Epitope Location or Sequence :
extracellular domain
Recommended Applications :
FACS,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
1374092 24979729 24714646 20599951 21624809 22430738 22761819 22844530 23552640 21659474 20093484 21682884 22421361 20231692 24274029 22876201
Immunofluorescence Pubmed IDs :
24979729 24714646 24695674 25395457 24879355 20599951 21624809 23466739 23995678 21356383 22430738 20855650 24006262 22761819 20407033 22020332 20690180 22421361 23840823 24274029 21435450 22566825 23063660 27694932 29666442 27002738
Western Blot Pubmed IDs :
1374092 24922070 22130923 23466739 23995678 23530051 22844530
FACS Pubmed IDs :
1374092 20599951 21596851 20308365 20308362 22430738 23592259 20479124 21436590 23963040 23169771 22566825
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Exp. Med. 176, 1477-1482.; J. Histochem. Cytochem. 40(5), 651-664.; Dev. Biol. 167(1), 294-306.; Development 128, 5181-5188.: EMBO J. 22(14), 3546-3556.; Am. J. Physiol. Lung Cell. Mol. Physiol. 290, 32-40.; Cancer Cell 9, 261-272. doi:10.1016/j.ccr.2006.03.10
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US