product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
integrin beta-3 (CD61), human
catalog :
7H2
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
7H2
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
more info or order :
citations: 14
Reference |
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Blue R, Murcia M, Karan C, Jirouskova M, Coller B. Application of high-throughput screening to identify a novel alphaIIb-specific small- molecule inhibitor of alphaIIbbeta3-mediated platelet interaction with fibrinogen. Blood. 2008;111:1248-56 pubmed
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Jirouskova M, Jaiswal J, Coller B. Ligand density dramatically affects integrin alpha IIb beta 3-mediated platelet signaling and spreading. Blood. 2007;109:5260-9 pubmed
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Mitchell W, Li J, Murcia M, Valentin N, Newman P, Coller B. Mapping early conformational changes in alphaIIb and beta3 during biogenesis reveals a potential mechanism for alphaIIbbeta3 adopting its bent conformation. Blood. 2007;109:3725-32 pubmed
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Nelson E, Li J, Mitchell W, Chandy M, Srivastava A, Coller B. Three novel beta-propeller mutations causing Glanzmann thrombasthenia result in production of normally stable pro-alphaIIb, but variably impaired progression of pro-alphaIIbbeta3 from endoplasmic reticulum to Golgi. J Thromb Haemost. 2005;3:2773-83 pubmed
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Mitchell W, Li J, Singh F, Michelson A, Bussel J, Coller B, et al. Two novel mutations in the alpha IIb calcium-binding domains identify hydrophobic regions essential for alpha IIbbeta 3 biogenesis. Blood. 2003;101:2268-76 pubmed
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Basani R, French D, Vilaire G, Brown D, Chen F, Coller B, et al. A naturally occurring mutation near the amino terminus of alphaIIb defines a new region involved in ligand binding to alphaIIbbeta3. Blood. 2000;95:180-8 pubmed
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Dangas G, Badimon J, Coller B, Fallon J, Sharma S, Hayes R, et al. Administration of abciximab during percutaneous coronary intervention reduces both ex vivo platelet thrombus formation and fibrin deposition: implications for a potential anticoagulant effect of abciximab. Arterioscler Thromb Vasc Biol. 1998;18:1342-9 pubmed
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Grimaldi C, Chen F, Wu C, Weiss H, Coller B, French D. Glycoprotein IIb Leu214Pro mutation produces glanzmann thrombasthenia with both quantitative and qualitative abnormalities in GPIIb/IIIa. Blood. 1998;91:1562-71 pubmed
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Grimaldi C, Chen F, Scudder L, Coller B, French D. A Cys374Tyr homozygous mutation of platelet glycoprotein IIIa (beta 3) in a Chinese patient with Glanzmann's thrombasthenia. Blood. 1996;88:1666-75 pubmed
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Reverter J, Beguin S, Kessels H, Kumar R, Hemker H, Coller B. Inhibition of platelet-mediated, tissue factor-induced thrombin generation by the mouse/human chimeric 7E3 antibody. Potential implications for the effect of c7E3 Fab treatment on acute thrombosis and "clinical restenosis". J Clin Invest. 1996;98:863-74 pubmed
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Kutok J, Coller B. Partial inhibition of platelet aggregation and fibrinogen binding by a murine monoclonal antibody to GPIIIa: requirement for antibody bivalency. Thromb Haemost. 1994;72:964-72 pubmed
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product information
Internal ID :
8892
Name :
7H2
Depositor Name :
Coller, B.S.
Depositor Institution :
Rockefeller University
Date Deposited :
5/2/13
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
integrin beta-3 (CD61), human
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Human,Primate
Species Tested (legacy) :
human and primate
Initial Publication Pubmed ID :
7537915
Depositor Notes (Special Instructions) :
7H2 reacts with alphaIIbbeta3 complex on platelets and has variable effects on platelet function. The epitope recognized by 7H2 is located on the beta3 PSI domain (unpublished mutational analysis).
Collections :
CD antigens,Cell adhesion,Cell signaling,Human
Search Keywords :
Coller, B.S., integrin beta-3 (CD61), human, Human, MIgG1, Human/Primate, ITGB3, GP3A, CD61, AB_2617394, monoclonal, Cell adhesion/Cluster Determinant antigens/Human/Cell signaling, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/Function Blocking/FACS
Antigen Molecular Weight :
Predicted; 87 kDa; Apparent: 90 kDa
Gene :
ITGB3
Alternate Gene Name(s) :
GP3A, CD61
Uniprot ID :
P05106
Antibody Registry ID :
AB_2617394
Immunogen :
Human platelets
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
X63-Ag8.653
Epitope Mapped :
Yes
Epitope Location or Sequence :
integrin beta3 PSI domain
Epitope Map PubMed ID :
24136164
Recommended Applications :
FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
9714143
Immunofluorescence Pubmed IDs :
24349096 17332246 17978171
Western Blot Pubmed IDs :
7537915 9473221 24349096 10607701 8781422 12424194
Immunoprecipitation Pubmed IDs :
9473221 17209052 16359515
Function Blocking Pubmed IDs :
7537915 8698879
FACS Pubmed IDs :
7537915 18175315
Additional Information :
7H2 recognizes free beta3 as weel as alphaVbeta3 and alphaIIbbeta3 and their pro-forms. In immunoblots, 7H2 doesn't recognize its target under reducing conditions.
DSHB Growth Medium :
Iscove's
References (legacy) :
Thromb. Haemost. 72(6), 964-972.; Blood 91(5), 1562-1571.
more info or order :
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Developmental Studies Hybridoma Bank
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