Fasciclin III (Drosophila) | Developmental Studies Hybridoma Bank 7G10 anti-Fasciclin III product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Fasciclin III (Drosophila)

catalog :

7G10 anti-Fasciclin III

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7G10 anti-Fasciclin III

reactivity :

fruit fly

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 41

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:50; loading ...; fig 3c	Hudry B, de Goeij E, Mineo A, Gaspar P, Hadjieconomou D, Studd C, et al. Sex Differences in Intestinal Carbohydrate Metabolism Promote Food Intake and Sperm Maturation. Cell. 2019;178:901-918.e16 pubmed publisher
immunohistochemistry; fruit fly; 1:50; loading ...; fig 3e	Lai C, Lin K, Kao S, Chen Y, Huang F, Hsu H. Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion. J Cell Biol. 2017;216:1439-1453 pubmed publisher
immunohistochemistry; fruit fly; fig 1d	Ulmschneider B, Grillo Hill B, Benitez M, Azimova D, Barber D, Nystul T. Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation. J Cell Biol. 2016;215:345-355 pubmed
immunohistochemistry; fruit fly; 1:10; fig 7i	Hanlon C, Andrew D. Drosophila FoxL1 non-autonomously coordinates organ placement during embryonic development. Dev Biol. 2016;419:273-284 pubmed publisher
immunohistochemistry; fruit fly; 1:50; fig 2d	Huang D, Li X, Sun L, Huang P, Ying H, Wang H, et al. Regulation of Hippo signalling by p38 signalling. J Mol Cell Biol. 2016;8:328-37 pubmed publisher
immunohistochemistry - paraffin section; fruit fly; 1:200; fig 2c	Woichansky I, Beretta C, Berns N, Riechmann V. Three mechanisms control E-cadherin localization to the zonula adherens. Nat Commun. 2016;7:10834 pubmed publisher
immunohistochemistry; fruit fly; 1:50	Bosveld F, Markova O, Guirao B, Martin C, Wang Z, Pierre A, et al. Epithelial tricellular junctions act as interphase cell shape sensors to orient mitosis. Nature. 2016;530:495-8 pubmed publisher
immunohistochemistry; fruit fly; 1:50	Bosveld F, Guirao B, Wang Z, Rivière M, Bonnet I, Graner F, et al. Modulation of junction tension by tumor suppressors and proto-oncogenes regulates cell-cell contacts. Development. 2016;143:623-34 pubmed publisher
immunohistochemistry; fruit fly; 1:10	Sollier K, GaudÃ© H, Chartier F, Laprise P. Rac1 controls epithelial tube length through the apical secretion and polarity pathways. Biol Open. 2015;5:49-54 pubmed publisher
immunohistochemistry; fruit fly; 1:20; fig s6	Campbell K, Casanova J. A role for E-cadherin in ensuring cohesive migration of a heterogeneous population of non-epithelial cells. Nat Commun. 2015;6:7998 pubmed publisher
immunohistochemistry; fruit fly; 1:50	Chen H, Huang Y, Pfeiffer B, Yao X, Lee T. An enhanced gene targeting toolkit for Drosophila: Golic+. Genetics. 2015;199:683-94 pubmed publisher
immunocytochemistry; fruit fly; 1:200	Chen D, Wu C, Zhao S, Geng Q, Gao Y, Li X, et al. Three RNA binding proteins form a complex to promote differentiation of germline stem cell lineage in Drosophila. PLoS Genet. 2014;10:e1004797 pubmed publisher
immunohistochemistry; fruit fly; 1:20; fig s15	Linnemannstöns K, Ripp C, Honemann Capito M, Brechtel Curth K, Hedderich M, Wodarz A. The PTK7-related transmembrane proteins off-track and off-track 2 are co-receptors for Drosophila Wnt2 required for male fertility. PLoS Genet. 2014;10:e1004443 pubmed publisher
	Wu L, Jia Q, Zhang X, Zhang X, Liu S, Park Y, et al. CYP303A1 has a conserved function in adult eclosion in Locusta migratoria and Drosophila melanogaster. Insect Biochem Mol Biol. 2019;113:103210 pubmed publisher
	Lu Y, Yao Y, Li Z. Ectopic Dpp signaling promotes stem cell competition through EGFR signaling in the Drosophila testis. Sci Rep. 2019;9:6118 pubmed publisher
	Jankovics F, Bence M, Sinka R, Faragó A, Bodai L, Pettkó Szandtner A, et al. Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation. Development. 2018;145: pubmed publisher
	Kline A, Curry T, Lewellyn L. The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber. Dev Biol. 2018;440:99-112 pubmed publisher
	Daniel E, DaudÃ© M, Kolotuev I, Charish K, Auld V, Le Borgne R. Coordination of Septate Junctions Assembly and Completion of Cytokinesis in Proliferative Epithelial Tissues. Curr Biol. 2018;28:1380-1391.e4 pubmed publisher
	Morrison C, Chen H, Cook T, Brown S, Treisman J. Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye. PLoS Genet. 2018;14:e1007173 pubmed publisher
	Dai W, Peterson A, Kenney T, Burrous H, Montell D. Quantitative microscopy of the Drosophila ovary shows multiple niche signals specify progenitor cell fate. Nat Commun. 2017;8:1244 pubmed publisher
	Lu K, Yamashita Y. Germ cell connectivity enhances cell death in response to DNA damage in the Drosophila testis. elife. 2017;6: pubmed publisher
	Redhai S, Hellberg J, Wainwright M, Perera S, Castellanos F, Kroeger B, et al. Regulation of Dense-Core Granule Replenishment by Autocrine BMP Signalling in Drosophila Secondary Cells. PLoS Genet. 2016;12:e1006366 pubmed publisher
	Lin T, Luo J, Shinomiya K, Ting C, Lu Z, Meinertzhagen I, et al. Mapping chromatic pathways in the Drosophila visual system. J Comp Neurol. 2016;524:213-27 pubmed publisher
	Lu C, Zhai B, Mauss A, Landgraf M, Gygi S, Van Vactor D. MicroRNA-8 promotes robust motor axon targeting by coordinate regulation of cell adhesion molecules during synapse development. Philos Trans R Soc Lond B Biol Sci. 2014;369: pubmed publisher
	Gonda R, Garlena R, Stronach B. Drosophila heat shock response requires the JNK pathway and phosphorylation of mixed lineage kinase at a conserved serine-proline motif. PLoS ONE. 2012;7:e42369 pubmed publisher
	Kim M, McGinnis W. Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier. Proc Natl Acad Sci U S A. 2011;108:650-5 pubmed publisher
	Voog J, D Alterio C, Jones D. Multipotent somatic stem cells contribute to the stem cell niche in the Drosophila testis. Nature. 2008;454:1132-6 pubmed publisher
	Rotte C, Walz B, Baumann O. Morphological and functional characterization of the thoracic portion of blowfly salivary glands. Arthropod Struct Dev. 2008;37:372-82 pubmed publisher
	Hozumi S, Maeda R, Taniguchi K, Kanai M, Shirakabe S, Sasamura T, et al. An unconventional myosin in Drosophila reverses the default handedness in visceral organs. Nature. 2006;440:798-802 pubmed
	Englund C, Lorén C, Grabbe C, Varshney G, Deleuil F, Hallberg B, et al. Jeb signals through the Alk receptor tyrosine kinase to drive visceral muscle fusion. Nature. 2003;425:512-6 pubmed
	Lorén C, Englund C, Grabbe C, Hallberg B, Hunter T, Palmer R. A crucial role for the Anaplastic lymphoma kinase receptor tyrosine kinase in gut development in Drosophila melanogaster. EMBO Rep. 2003;4:781-6 pubmed
	Song X, Xie T. DE-cadherin-mediated cell adhesion is essential for maintaining somatic stem cells in the Drosophila ovary. Proc Natl Acad Sci U S A. 2002;99:14813-8 pubmed
	Baumann O. Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia. Exp Cell Res. 2001;270:176-87 pubmed
	Alexandre C, Lecourtois M, Vincent J. Wingless and Hedgehog pattern Drosophila denticle belts by regulating the production of short-range signals. Development. 1999;126:5689-98 pubmed
	Godt D, Tepass U. Drosophila oocyte localization is mediated by differential cadherin-based adhesion. Nature. 1998;395:387-91 pubmed
	Wolf B, Seeger M, Chiba A. Commissureless endocytosis is correlated with initiation of neuromuscular synaptogenesis. Development. 1998;125:3853-63 pubmed
	Kose H, Rose D, Zhu X, Chiba A. Homophilic synaptic target recognition mediated by immunoglobulin-like cell adhesion molecule Fasciclin III. Development. 1997;124:4143-52 pubmed
	Fambrough D, Pan D, Rubin G, Goodman C. The cell surface metalloprotease/disintegrin Kuzbanian is required for axonal extension in Drosophila. Proc Natl Acad Sci U S A. 1996;93:13233-8 pubmed
	GONCZY P, DiNardo S. The germ line regulates somatic cyst cell proliferation and fate during Drosophila spermatogenesis. Development. 1996;122:2437-47 pubmed
	Desai C, Gindhart J, Goldstein L, Zinn K. Receptor tyrosine phosphatases are required for motor axon guidance in the Drosophila embryo. Cell. 1996;84:599-609 pubmed
	Patel N, Snow P, Goodman C. Characterization and cloning of fasciclin III: a glycoprotein expressed on a subset of neurons and axon pathways in Drosophila. Cell. 1987;48:975-88 pubmed

product information

Internal ID :

3592

Name :

7G10 anti-Fasciclin III

Depositor Name :

Goodman, C.

Depositor Institution :

University of California, Berkeley

Date Deposited :

2/1/94

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Fasciclin III (Drosophila)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Drosophila

Initial Publication Pubmed ID :

3548998

Depositor Notes (Special Instructions) :

Fas III is expressed on a subset of neurons and axons in the CNS, patches of ectoderm, ectodermal stripes, parts of the hindgut and foregut, visceral mesoderm, and the salivary gland. The antibody can be used to stain dissections without detergent as the epitope is on the extracellular domain of the protein. After 10 hrs in development, ectodermal staining will obscure CNS in whole mount preps and therefore dissected preparations are recommended for embryos staining past this age. During larval development, staining is present on a subset of neurons, the epithelial layer of the discs and a cyclone fence pattern in the eye disc. It is especially useful for following the development of the RP1 and RP3 neurons. The antibody works for affinity purification but does not work for immunoprecipitation or western.

Collections :

Cell adhesion,Drosophila antigens,Neurodevelopment,Skeletal muscle

Search Keywords :

Goodman, Corey, Fasciclin III (Drosophila), Drosophila, MIgG2a, Drosophila, Fas3, Fasciclin 3, , monoclonal, AB_528238,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Antigen Molecular Weight :

Multiple isoforms; Apparent: 46, 59, 66, and 80 kDa

Gene :

Fas3

Alternate Gene Name(s) :

Fasciclin 3

Uniprot ID :

P15278

Antibody Registry ID :

AB_528238

Immunogen :

Membrane preparation from nerve cord and cultures of primary neurons (10-13 hr development).

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

Immunohistochemistry Pubmed IDs :

3548998 8917574 9374410 8598046 31422152

Immunofluorescence Pubmed IDs :

10572045 21187384 22848763 25135978 18406207 14523447 9759729 12855999 8756289 18641633 9729493 12393817 16598258 27727275 28809158 29093440 26179639 29324767 29706514 29753016 30389853 30992503

Immunoprecipitation Pubmed IDs :

3548998

Pubmed IDs :

25135978 11640882

Additional Information :

This antibody may work for Immunoprecipitation. While the Depositor clearly stated that it doesn't work for immunoprecipitation (see Depositor Notes), the Initial Publication, in which the Depositor is the last author, reports the succesful use of 7G10 for the immunoprecipitation of Fasciclin III.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 48, 975-988.; Development 126, 5689-5698.; Exp. Cell Res. 270, 176-187.; Arthropod. Struct. Dev. 37, 372-382.; PNAS 108(2), 650-655.; PLoS One 7(7):e42369. Doi:10.1371/journal.pone.0042369.; Phil. Trans. R. Soc. B doi:10.1098/rstb.2013.0517.

more info or order :

Developmental Studies Hybridoma Bank product webpage