product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
FMR1
catalog :
7G1-1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
7G1-1
reactivity :
mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, RNA immunoprecipitation, western blot knockout validation
citations: 23
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; loading ...; fig 1a
  • RNA immunoprecipitation; mouse; loading ...; fig 1a
Chmielewska J, Kuzniewska B, Milek J, Urbanska K, Dziembowska M. Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage. Mol Neurobiol. 2019;56:2741-2759 pubmed publisher
  • western blot knockout validation; mouse; fig 2c
Xu X, Zong R, Li Z, Biswas M, Fang Z, Nelson D, et al. FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124. J Neurosci. 2011;31:13705-9 pubmed publisher
Zhang P, Abdelmohsen K, Liu Y, Tominaga Yamanaka K, Yoon J, Ioannis G, et al. Novel RNA- and FMRP-binding protein TRF2-S regulates axonal mRNA transport and presynaptic plasticity. Nat Commun. 2015;6:8888 pubmed publisher
Ferron L, Nieto Rostro M, Cassidy J, Dolphin A. Fragile X mental retardation protein controls synaptic vesicle exocytosis by modulating N-type calcium channel density. Nat Commun. 2014;5:3628 pubmed publisher
Lindsay A, McCaffrey M. Myosin Va is required for the transport of fragile X mental retardation protein (FMRP) granules. Biol Cell. 2014;106:57-71 pubmed publisher
Bernard P, Castano A, O Leary H, Simpson K, Browning M, Benke T. Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures. Neurobiol Dis. 2013;59:1-17 pubmed publisher
El Fatimy R, Tremblay S, Dury A, Solomon S, De Koninck P, Schrader J, et al. Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected]. PLoS ONE. 2012;7:e39338 pubmed publisher
Chi S, Hannon G, Darnell R. An alternative mode of microRNA target recognition. Nat Struct Mol Biol. 2012;19:321-7 pubmed publisher
Baez M, Luchelli L, Maschi D, Habif M, Pascual M, Thomas M, et al. Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation. J Cell Biol. 2011;195:1141-57 pubmed publisher
Darnell J, Van Driesche S, Zhang C, Hung K, Mele A, Fraser C, et al. FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism. Cell. 2011;146:247-61 pubmed publisher
Saffary R, Xie Z. FMRP regulates the transition from radial glial cells to intermediate progenitor cells during neocortical development. J Neurosci. 2011;31:1427-39 pubmed publisher
Zhao W, Chuang S, Bianchi R, Wong R. Dual regulation of fragile X mental retardation protein by group I metabotropic glutamate receptors controls translation-dependent epileptogenesis in the hippocampus. J Neurosci. 2011;31:725-34 pubmed publisher
Pacey L, Doss L, Cifelli C, van der Kooy D, Heximer S, Hampson D. Genetic deletion of regulator of G-protein signaling 4 (RGS4) rescues a subset of fragile X related phenotypes in the FMR1 knockout mouse. Mol Cell Neurosci. 2011;46:563-72 pubmed publisher
Gross C, Nakamoto M, Yao X, Chan C, Yim S, Ye K, et al. Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome. J Neurosci. 2010;30:10624-38 pubmed publisher
Strumbos J, Brown M, Kronengold J, Polley D, Kaczmarek L. Fragile X mental retardation protein is required for rapid experience-dependent regulation of the potassium channel Kv3.1b. J Neurosci. 2010;30:10263-71 pubmed publisher
Zang J, Nosyreva E, Spencer C, Volk L, Musunuru K, Zhong R, et al. A mouse model of the human Fragile X syndrome I304N mutation. PLoS Genet. 2009;5:e1000758 pubmed publisher
Christie S, Akins M, Schwob J, Fallon J. The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits. J Neurosci. 2009;29:1514-24 pubmed publisher
Iacoangeli A, Rozhdestvensky T, Dolzhanskaya N, Tournier B, Schütt J, BROSIUS J, et al. Reply to Bagni: On BC1 RNA and the fragile X mental retardation protein. Proc Natl Acad Sci U S A. 2008;105:E29 pubmed publisher
Lugli G, Torvik V, Larson J, Smalheiser N. Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem. 2008;106:650-61 pubmed publisher
Westmark C, Malter J. FMRP mediates mGluR5-dependent translation of amyloid precursor protein. PLoS Biol. 2007;5:e52 pubmed
Wulczyn F, Smirnova L, Rybak A, Brandt C, Kwidzinski E, Ninnemann O, et al. Post-transcriptional regulation of the let-7 microRNA during neural cell specification. FASEB J. 2007;21:415-26 pubmed
Price T, Flores C, Cervero F, Hargreaves K. The RNA binding and transport proteins staufen and fragile X mental retardation protein are expressed by rat primary afferent neurons and localize to peripheral and central axons. Neuroscience. 2006;141:2107-16 pubmed
Brown V, Jin P, Ceman S, Darnell J, O Donnell W, Tenenbaum S, et al. Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome. Cell. 2001;107:477-87 pubmed
product information
Internal ID :
963
Name :
7G1-1
Depositor Name :
Warren, S.T.
Depositor Institution :
Emory University
Date Deposited :
3/7/03
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
FMR1
Antigen Species :
Mouse
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Mouse,Rat
Species Tested (legacy) :
mouse, rat, not human
Initial Publication Pubmed ID :
11719188
Collections :
Cell signaling,Human,Neurodevelopment
Search Keywords :
Warren, Stephen T., FMR1, Mouse, MIgG2b, Mouse/Rat, Fmr1, FMRP, Fmr-1, AB_528251, monoclonal
Antigen Molecular Weight :
~70-80kDa
Gene :
Fmr1
Alternate Gene Name(s) :
FMRP, Fmr-1
Uniprot ID :
P35922
Antibody Registry ID :
AB_528251
Immunogen :
Fmrp-6X-his fusion protein.
Clonality :
Monoclonal
Myeloma Strain :
Sp2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
a.a. 354-368 [seq: KHLDTKENTHFSQPN}
Epitope Map PubMed ID :
11719188
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
19193898
Immunofluorescence Pubmed IDs :
24175909 22201125 19193898
Western Blot Pubmed IDs :
22737234 21957233 21273427 21215802 22737234 16809002 20011099
Immunoprecipitation Pubmed IDs :
11719188 22737234 24709664 23831253 20685971 22737234 21784246 21228181 22343717 20702695 17298186 18511554 20011099 18410515 30056576
Gel Supershift Pubmed IDs :
17167072
DSHB Growth Medium :
Iscove's
References (legacy) :
Cell 107, 477-487.; PLoS One 7(6):e39338.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US