product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
integrin beta-1 /CD29
catalog :
7E2
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
7E2
application :
western blot, immunocytochemistry, immunoprecipitation, FACS
citations: 11
Reference
Hang Q, Isaji T, Hou S, Im S, Fukuda T, Gu J. Integrin α5 Suppresses the Phosphorylation of Epidermal Growth Factor Receptor and Its Cellular Signaling of Cell Proliferation via N-Glycosylation. J Biol Chem. 2015;290:29345-60 pubmed publisher
Honda S, Shirotani Ikejima H, Tadokoro S, Maeda Y, Kinoshita T, Tomiyama Y, et al. Integrin-linked kinase associated with integrin activation. Blood. 2009;113:5304-13 pubmed publisher
Larrucea S, Butta N, Arias Salgado E, Alonso Martin S, Ayuso M, Parrilla R. Expression of podocalyxin enhances the adherence, migration, and intercellular communication of cells. Exp Cell Res. 2008;314:2004-15 pubmed publisher
Isaji T, Sato Y, Zhao Y, Miyoshi E, Wada Y, Taniguchi N, et al. N-glycosylation of the beta-propeller domain of the integrin alpha5 subunit is essential for alpha5beta1 heterodimerization, expression on the cell surface, and its biological function. J Biol Chem. 2006;281:33258-67 pubmed
Thodeti C, Fröhlich C, Nielsen C, Takada Y, Fassler R, Albrechtsen R, et al. ADAM12-mediated focal adhesion formation is differently regulated by beta1 and beta3 integrins. FEBS Lett. 2005;579:5589-95 pubmed
Goel H, Fornaro M, Moro L, Teider N, Rhim J, King M, et al. Selective modulation of type 1 insulin-like growth factor receptor signaling and functions by beta1 integrins. J Cell Biol. 2004;166:407-18 pubmed
Cook G, Longhurst C, Grgurevich S, Cholera S, Crossno J, Jennings L. Identification of CD9 extracellular domains important in regulation of CHO cell adhesion to fibronectin and fibronectin pericellular matrix assembly. Blood. 2002;100:4502-11 pubmed
Laukaitis C, Webb D, Donais K, Horwitz A. Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. J Cell Biol. 2001;153:1427-40 pubmed
Klass C, Couchman J, Woods A. Control of extracellular matrix assembly by syndecan-2 proteoglycan. J Cell Sci. 2000;113 ( Pt 3):493-506 pubmed
Raub T, Kuentzel S. Kinetic and morphological evidence for endocytosis of mammalian cell integrin receptors by using an anti-fibronectin receptor beta subunit monoclonal antibody. Exp Cell Res. 1989;184:407-26 pubmed
Brown P, Juliano R. Monoclonal antibodies to distinctive epitopes on the alpha and beta subunits of the fibronectin receptor. Exp Cell Res. 1988;177:303-18 pubmed
product information
Internal ID :
2767
Name :
7E2
Depositor Name :
Juliano, R.L.
Depositor Institution :
UNC - Chapel Hill
Depositor Website :
https://www.med.unc.edu/pharm/people/joint-faculty/rudolph-l-juliano-1
Date Deposited :
6/8/94
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
integrin beta-1 /CD29
Antigen Species :
Chinese hamster
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Chinese Hamster
Species Tested (legacy) :
No cross reaction
Initial Publication Pubmed ID :
2455654
Depositor Notes (Special Instructions) :
This antibody recognizes a confirmational epitope on the beta subunit that is dependent upon disulfide bonding. 7E2 fails to recognize B1 integrin by immunoblot on WISH, HDF, BHK, NRK and NIH 3T3 cells.
Collections :
CD antigens,Cell adhesion,Cell signaling,Extracellular matrix proteins
Search Keywords :
Juliano, R.L., integrin beta-1 /CD29, Chinese hamster , MIgG1, kappa light chain, Chinese hamster , I79_016350 , , AB_532374, Monoclonal, Cell adhesion/Cluster determinant antigens, Immunoprecipitation/Western blot/FACS/Immunofluorescence
Antigen Molecular Weight :
110 kDa (native)
Gene :
I79_016350
Uniprot ID :
G3HZ55
Antibody Registry ID :
AB_532374
Immunogen :
Purified fibronectin receptor from CHO cells
Clonality :
Monoclonal
Myeloma Strain :
P2x63.Ag8.63
Epitope Mapped :
No
Recommended Applications :
FACS,Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Blots nonreduced protein
Immunofluorescence Pubmed IDs :
12453879 11425873 16959765 18456258 2530101
Western Blot Pubmed IDs :
2455654 26483551 18456258 2530101
Immunoprecipitation Pubmed IDs :
2455654 12453879 16959765 2530101
ELISA Pubmed IDs :
2530101
FACS Pubmed IDs :
16213489 10639336 15289498 19299337
Additional Information :
RRID:AB_532374
DSHB Growth Medium :
RPMI
References (legacy) :
Exp. Cell Res. 177, 303-318.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US