Nuclear factor of activated T-cells, cytoplasmic 1 | Developmental Studies Hybridoma Bank 7A6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Nuclear factor of activated T-cells, cytoplasmic 1

catalog :

7A6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7A6

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:50; fig 5d	von Moltke J, O Leary C, Barrett N, Kanaoka Y, Austen K, Locksley R. Leukotrienes provide an NFAT-dependent signal that synergizes with IL-33 to activate ILC2s. J Exp Med. 2017;214:27-37 pubmed publisher
western blot; mouse; fig 6c	Zou Q, Jin J, Xiao Y, Hu H, Zhou X, Jie Z, et al. T cell development involves TRAF3IP3-mediated ERK signaling in the Golgi. J Exp Med. 2015;212:1323-36 pubmed publisher
western blot; mouse; 1:1000; loading ...	Serrano PÃ©rez M, FernÃ¡ndez M, Neria F, BerjÃ³n Otero M, Doncel PÃ©rez E, Cano E, et al. NFAT transcription factors regulate survival, proliferation, migration, and differentiation of neural precursor cells. Glia. 2015;63:987-1004 pubmed publisher
	Canté Barrett K, Winslow M, Crabtree G. Selective role of NFATc3 in positive selection of thymocytes. J Immunol. 2007;179:103-10 pubmed
	Matsuo K, Galson D, Zhao C, Peng L, Laplace C, Wang K, et al. Nuclear factor of activated T-cells (NFAT) rescues osteoclastogenesis in precursors lacking c-Fos. J Biol Chem. 2004;279:26475-80 pubmed
	Sheridan C, Heist E, Beals C, Crabtree G, Gardner P. Protein kinase A negatively modulates the nuclear accumulation of NF-ATc1 by priming for subsequent phosphorylation by glycogen synthase kinase-3. J Biol Chem. 2002;277:48664-76 pubmed
	Graef I, Chen F, Chen L, Kuo A, Crabtree G. Signals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature. Cell. 2001;105:863-75 pubmed
	Mosieniak G, Pyrzynska B, Kaminska B. Nuclear factor of activated T cells (NFAT) as a new component of the signal transduction pathway in glioma cells. J Neurochem. 1998;71:134-41 pubmed
	Holsinger L, Graef I, Swat W, Chi T, Bautista D, Davidson L, et al. Defects in actin-cap formation in Vav-deficient mice implicate an actin requirement for lymphocyte signal transduction. Curr Biol. 1998;8:563-72 pubmed
	de la Pompa J, Timmerman L, Takimoto H, Yoshida H, Elia A, Samper E, et al. Role of the NF-ATc transcription factor in morphogenesis of cardiac valves and septum. Nature. 1998;392:182-6 pubmed
	Timmerman L, Healy J, Ho S, Chen L, Goodnow C, Crabtree G. Redundant expression but selective utilization of nuclear factor of activated T cells family members. J Immunol. 1997;159:2735-40 pubmed
	Healy J, Dolmetsch R, Timmerman L, Cyster J, Thomas M, Crabtree G, et al. Different nuclear signals are activated by the B cell receptor during positive versus negative signaling. Immunity. 1997;6:419-28 pubmed
	Beals C, Clipstone N, Ho S, Crabtree G. Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction. Genes Dev. 1997;11:824-34 pubmed
	Timmerman L, Clipstone N, Ho S, Northrop J, Crabtree G. Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression. Nature. 1996;383:837-40 pubmed
	Northrop J, Ho S, Chen L, Thomas D, Timmerman L, Nolan G, et al. NF-AT components define a family of transcription factors targeted in T-cell activation. Nature. 1994;369:497-502 pubmed
	Ho S, Thomas D, Timmerman L, Li X, Francke U, Crabtree G. NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity. J Biol Chem. 1995;270:19898-907 pubmed

product information

Internal ID :

4887

Name :

7A6

Depositor Name :

Crabtree, G.R.

Depositor Institution :

Beckman Center, Stanford University

Date Deposited :

9/17/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Nuclear factor of activated T-cells, cytoplasmic 1

Antigen Species :

human

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Human,Mouse,Rat

Species Tested (legacy) :

mouse and human

Initial Publication Pubmed ID :

8202141

Collections :

Human,Transcription factors

Search Keywords :

Crabtree, G.R., Nuclear factor of activated T-cells, cytoplasmic 1, human, MIgG1, kappa light chain, mouse/human/rat, NFATC1, NF-ATC, NFAT2, NFATc, AB_2152505, monoclonal, human/transcription factors, Immunohistochemistry/Immunofluorescence/Western Blot/mmunoprecipitation/Gel Supershift

Antigen Molecular Weight :

Predicted: multiple isoforms from 39 to 101 kDa; Apparent: phosphorylated form (cytoplasmic) at 150, 120, and 80 kDa, and dephosphorylated form (nuclear) at 120, 80, and 68 kDa

Gene :

NFATC1

Alternate Gene Name(s) :

NF-ATC, NFAT2, NFATc

Uniprot ID :

O95644

Antibody Registry ID :

AB_2152505

Immunogen :

Recombinant GST/NFATC1 (aa 1-654 of isoform A-alpha) fusion protein

Immunogen Sequence :

partial protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 186-380

Epitope Map PubMed ID :

9515963 9648859

Recommended Applications :

Gel Supershift,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

9515963

Immunofluorescence Pubmed IDs :

8893011 15073183 9300694 9515963 9601640 12351631 11439183 9648859

Western Blot Pubmed IDs :

15073183 9106655 9300694 9515963 7650004 9601640 9133421 12351631 17579027 9648859

Immunoprecipitation Pubmed IDs :

12351631

Gel Supershift Pubmed IDs :

8202141 15073183 9300694 7650004

DSHB Growth Medium :

Iscove's

References (legacy) :

Nature 369, 497-502.; Nature 383, 837-840.; Science 275, 1930-1933.

more info or order :

Developmental Studies Hybridoma Bank product webpage