product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Nkx2.2 transcription factor
catalog :
74.5A5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
74.5A5
reactivity :
African green monkey, human, mouse, rat, chicken, Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 66
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:50; loading ...; fig s1a
Luisier R, Tyzack G, Hall C, Mitchell J, Devine H, Taha D, et al. Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALS. Nat Commun. 2018;9:2010 pubmed publisher
  • immunocytochemistry; human; 1:250; fig e1c
Quadrato G, Nguyen T, Macosko E, Sherwood J, Min Yang S, Berger D, et al. Cell diversity and network dynamics in photosensitive human brain organoids. Nature. 2017;545:48-53 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 1r
Himmels P, Paredes I, Adler H, Karakatsani A, Luck R, Marti H, et al. Motor neurons control blood vessel patterning in the developing spinal cord. Nat Commun. 2017;8:14583 pubmed publisher
  • immunocytochemistry; mouse; 1:100; loading ...; fig 4i
Fujii K, Shi Z, Zhulyn O, Denans N, Barna M. Pervasive translational regulation of the cell signalling circuitry underlies mammalian development. Nat Commun. 2017;8:14443 pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig 4H
  • immunocytochemistry; African green monkey; 1:50; loading ...; fig 1G
Yamashita T, Miyamoto Y, Bando Y, Ono T, Kobayashi S, Doi A, et al. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells. PLoS ONE. 2017;12:e0171947 pubmed publisher
  • immunohistochemistry - free floating section; Xenopus laevis; 1:500; loading ...; tbl 2
Morona R, Ferran J, Puelles L, González A. Gene expression analysis of developing cell groups in the pretectal region of Xenopus laevis. J Comp Neurol. 2017;525:715-752 pubmed publisher
  • immunohistochemistry; mouse; 1:5; loading ...; fig 4
Weng C, Ding M, Chang L, Ren M, Zhang H, Lu Z, et al. Ankfy1 is dispensable for neural stem/precursor cell development. Neural Regen Res. 2016;11:1804-1809 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:10; loading ...; fig 5c
Li Y, Tzatzalos E, Kwan K, Grumet M, Cai L. Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development. Sci Rep. 2016;6:38665 pubmed publisher
  • immunohistochemistry; mouse; fig s1
Airik R, Schueler M, Airik M, Cho J, Ulanowicz K, Porath J, et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS ONE. 2016;11:e0156081 pubmed publisher
  • immunocytochemistry; human; 1:100; fig s1
Xue Y, Qian H, Hu J, Zhou B, Zhou Y, Hu X, et al. Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells. Nat Neurosci. 2016;19:807-15 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 1
Douvaras P, Rusielewicz T, Kim K, Haines J, CASACCIA P, Fossati V. Epigenetic Modulation of Human Induced Pluripotent Stem Cell Differentiation to Oligodendrocytes. Int J Mol Sci. 2016;17: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig st4
Dooves S, Bugiani M, Postma N, Polder E, Land N, Horan S, et al. Astrocytes are central in the pathomechanisms of vanishing white matter. J Clin Invest. 2016;126:1512-24 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 1
Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 5 ug/ml; fig 2
Misuraca K, Hu G, Barton K, Chung A, Becher O. A Novel Mouse Model of Diffuse Intrinsic Pontine Glioma Initiated in Pax3-Expressing Cells. Neoplasia. 2016;18:60-70 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:1000; loading ...; tbl 1
Pirson M, Debrulle S, Clippe A, Clotman F, Knoops B. Thioredoxin-2 Modulates Neuronal Programmed Cell Death in the Embryonic Chick Spinal Cord in Basal and Target-Deprived Conditions. PLoS ONE. 2015;10:e0142280 pubmed publisher
  • immunohistochemistry; chicken; 1:80; fig 1
Gallina D, Zelinka C, Cebulla C, Fischer A. Activation of glucocorticoid receptors in Müller glia is protective to retinal neurons and suppresses microglial reactivity. Exp Neurol. 2015;273:114-25 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig s4
Parchem R, Moore N, Fish J, Parchem J, Braga T, Shenoy A, et al. miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability. Cell Rep. 2015;12:760-73 pubmed publisher
  • immunohistochemistry; mouse; 1:50
Huettl R, Eckstein S, Stahl T, Petricca S, Ninkovic J, Götz M, et al. Functional dissection of the Pax6 paired domain: Roles in neural tube patterning and peripheral nervous system development. Dev Biol. 2016;413:86-103 pubmed publisher
  • immunohistochemistry; mouse; 1:250
Wende C, Zoubaa S, Blak A, Echevarria D, Martinez S, Guillemot F, et al. Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis. PLoS ONE. 2015;10:e0127681 pubmed publisher
  • immunohistochemistry; mouse; 1:1000
Jarrar W, Vauti F, Arnold H, Holz A. Generation of a Nkx2.2(Cre) knock-in mouse line: Analysis of cell lineages in the central nervous system. Differentiation. 2015;89:70-6 pubmed publisher
  • immunohistochemistry; mouse; fig s5
Eisner A, Pazyra Murphy M, Durresi E, Zhou P, Zhao X, Chadwick E, et al. The Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis. Dev Cell. 2015;33:22-35 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig s5
Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, et al. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis. Nat Commun. 2015;6:6366 pubmed publisher
  • immunohistochemistry; mouse; fig s12
Shi X, Zhang Z, Zhan X, Cao M, Satoh T, Akira S, et al. An epigenetic switch induced by Shh signalling regulates gene activation during development and medulloblastoma growth. Nat Commun. 2014;5:5425 pubmed publisher
  • immunohistochemistry; mouse; fig 3a
Goggolidou P, Stevens J, Agueci F, Keynton J, Wheway G, Grimes D, et al. ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis. Development. 2014;141:3966-77 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 6j
Zou M, Luo H, Xiang M. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn. 2015;244:86-97 pubmed publisher
  • immunocytochemistry; mouse; 1:10; fig 3
Alfaro A, Roberts B, Kwong L, Bijlsma M, Roelink H. Ptch2 mediates the Shh response in Ptch1-/- cells. Development. 2014;141:3331-9 pubmed publisher
  • immunohistochemistry; mouse; 1:10
Tiwari S, Dharmarajan S, Shivanna M, Otteson D, Belecky Adams T. Histone deacetylase expression patterns in developing murine optic nerve. BMC Dev Biol. 2014;14:30 pubmed publisher
  • immunohistochemistry - free floating section; Xenopus laevis; 1:500
Dominguez L, González A, Moreno N. Characterization of the hypothalamus of Xenopus laevis during development. II. The basal regions. J Comp Neurol. 2014;522:1102-31 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:25
Carrasco M, Delgado I, Soria B, Martin F, Rojas A. GATA4 and GATA6 control mouse pancreas organogenesis. J Clin Invest. 2012;122:3504-15 pubmed publisher
Neumann B, Baror R, Zhao C, SEGEL M, Dietmann S, Rawji K, et al. Metformin Restores CNS Remyelination Capacity by Rejuvenating Aged Stem Cells. Cell Stem Cell. 2019;25:473-485.e8 pubmed publisher
Campbell S, McDonald C, Krentz N, Lynn F, Hoffman B. TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation. Cell Rep. 2019;28:1830-1844.e6 pubmed publisher
Nam H, Jeon S, An H, Yoo J, Lee H, Lee S, et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification. elife. 2019;8: pubmed publisher
Yen Y, Hsieh W, Tsai Y, Lü Y, Liau E, Hsu H, et al. Dlk1-Dio3 locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identity. elife. 2018;7: pubmed publisher
Lager A, Corradin O, Cregg J, Elitt M, Shick H, Clayton B, et al. Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells. Nat Commun. 2018;9:3708 pubmed publisher
Lu T, Heyne S, Dror E, Casas E, Leonhardt L, Boenke T, et al. The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes. Cell Metab. 2018;27:1294-1308.e7 pubmed publisher
Thawani A, Sirohi D, Kuhn R, Fekete D. Zika Virus Can Strongly Infect and Disrupt Secondary Organizers in the Ventricular Zone of the Embryonic Chicken Brain. Cell Rep. 2018;23:692-700 pubmed publisher
Memon B, Karam M, Al Khawaga S, Abdelalim E. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1. Stem Cell Res Ther. 2018;9:15 pubmed publisher
Pusapati G, Kong J, Patel B, Krishnan A, Sagner A, Kinnebrew M, et al. CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog. Dev Cell. 2018;44:113-129.e8 pubmed publisher
Wang C, Li J, Meng Q, Wang B. Three Tctn proteins are functionally conserved in the regulation of neural tube patterning and Gli3 processing but not ciliogenesis and Hedgehog signaling in the mouse. Dev Biol. 2017;430:156-165 pubmed publisher
Varadarajan S, Butler S. Netrin1 establishes multiple boundaries for axon growth in the developing spinal cord. Dev Biol. 2017;430:177-187 pubmed publisher
Hoelzl M, Heby Henricson K, Gerling M, Dias J, Kuiper R, Trünkle C, et al. Differential requirement of SUFU in tissue development discovered in a hypomorphic mouse model. Dev Biol. 2017;429:132-146 pubmed publisher
Cai H, Liu A. Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord. Dev Biol. 2017;432:72-85 pubmed publisher
Sabo J, Heine V, Silbereis J, Schirmer L, Levison S, Rowitch D. Olig1 is required for noggin-induced neonatal myelin repair. Ann Neurol. 2017;81:560-571 pubmed publisher
Wang H, Matise M. Tcf7l2/Tcf4 Transcriptional Repressor Function Requires HDAC Activity in the Developing Vertebrate CNS. PLoS ONE. 2016;11:e0163267 pubmed publisher
Md Moin A, Dhawan S, Shieh C, Butler P, Cory M, Butler A. Increased Hormone-Negative Endocrine Cells in the Pancreas in Type 1 Diabetes. J Clin Endocrinol Metab. 2016;101:3487-96 pubmed publisher
Laumonnerie C, Tong Y, Alstermark H, Wilson S. Commissural axonal corridors instruct neuronal migration in the mouse spinal cord. Nat Commun. 2015;6:7028 pubmed publisher
Caballero I, Manuel M, Molinek M, Quintana Urzainqui I, Mi D, Shimogori T, et al. Cell-autonomous repression of Shh by transcription factor Pax6 regulates diencephalic patterning by controlling the central diencephalic organizer. Cell Rep. 2014;8:1405-18 pubmed publisher
Pifarre P, Gutierrez Mecinas M, Prado J, Usero L, Roura Mir C, Giralt M, et al. Phosphodiesterase 5 inhibition at disease onset prevents experimental autoimmune encephalomyelitis progression through immunoregulatory and neuroprotective actions. Exp Neurol. 2014;251:58-71 pubmed publisher
Achim K, Peltopuro P, Lahti L, Tsai H, Zachariah A, Astrand M, et al. The role of Tal2 and Tal1 in the differentiation of midbrain GABAergic neuron precursors. Biol Open. 2013;2:990-7 pubmed publisher
Tang M, Luo S, Tang V, Huang E. Temporal and spatial requirements of Smoothened in ventral midbrain neuronal development. Neural Dev. 2013;8:8 pubmed publisher
Kobayashi N, Homma S, Okada T, Masuda T, Sato N, Nishiyama K, et al. Elucidation of target muscle and detailed development of dorsal motor neurons in chick embryo spinal cord. J Comp Neurol. 2013;521:2987-3002 pubmed publisher
Mukhopadhyay S, Wen X, Ratti N, Loktev A, Rangell L, Scales S, et al. The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling. Cell. 2013;152:210-23 pubmed publisher
Joven A, Morona R, González A, Moreno N. Expression patterns of Pax6 and Pax7 in the adult brain of a urodele amphibian, Pleurodeles waltl. J Comp Neurol. 2013;521:2088-124 pubmed publisher
Han L, Pan Y, Wang B. Small ubiquitin-like Modifier (SUMO) modification inhibits GLI2 protein transcriptional activity in vitro and in vivo. J Biol Chem. 2012;287:20483-9 pubmed publisher
Rabe Bernhardt N, Memic F, Gezelius H, Thiebes A, Vallstedt A, Kullander K. DCC mediated axon guidance of spinal interneurons is essential for normal locomotor central pattern generator function. Dev Biol. 2012;366:279-89 pubmed publisher
Najm F, Zaremba A, Caprariello A, Nayak S, Freundt E, Scacheri P, et al. Rapid and robust generation of functional oligodendrocyte progenitor cells from epiblast stem cells. Nat Methods. 2011;8:957-62 pubmed publisher
Skaggs K, Martin D, Novitch B. Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling. Development. 2011;138:3199-211 pubmed publisher
Honig G, Liou A, Berger M, German M, Tecott L. Precise pattern of recombination in serotonergic and hypothalamic neurons in a Pdx1-cre transgenic mouse line. J Biomed Sci. 2010;17:82 pubmed publisher
Peltopuro P, Kala K, Partanen J. Distinct requirements for Ascl1 in subpopulations of midbrain GABAergic neurons. Dev Biol. 2010;343:63-70 pubmed publisher
Cameron D, Pennimpede T, Petkovich M. Tulp3 is a critical repressor of mouse hedgehog signaling. Dev Dyn. 2009;238:1140-9 pubmed publisher
Talbott J, Loy D, Liu Y, Qiu M, Bunge M, Rao M, et al. Endogenous Nkx2.2+/Olig2+ oligodendrocyte precursor cells fail to remyelinate the demyelinated adult rat spinal cord in the absence of astrocytes. Exp Neurol. 2005;192:11-24 pubmed
Ju M, Aroca P, Luo J, Puelles L, Redies C. Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate. Neuroscience. 2004;128:785-96 pubmed
Takebayashi H, Nabeshima Y, Yoshida S, Chisaka O, Ikenaka K, Nabeshima Y. The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages. Curr Biol. 2002;12:1157-63 pubmed
Takebayashi H, Ohtsuki T, Uchida T, Kawamoto S, Okubo K, Ikenaka K, et al. Non-overlapping expression of Olig3 and Olig2 in the embryonic neural tube. Mech Dev. 2002;113:169-74 pubmed
Soula C, Danesin C, Kan P, Grob M, Poncet C, Cochard P. Distinct sites of origin of oligodendrocytes and somatic motoneurons in the chick spinal cord: oligodendrocytes arise from Nkx2.2-expressing progenitors by a Shh-dependent mechanism. Development. 2001;128:1369-79 pubmed
Ericson J, Rashbass P, Schedl A, Brenner Morton S, Kawakami A, van Heyningen V, et al. Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling. Cell. 1997;90:169-80 pubmed
product information
Internal ID :
2440
Name :
74.5A5
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
HHMI/Columbia University
Date Deposited :
2/12/99
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Nkx2.2 transcription factor
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b, kappa light chain
Positive Tested Species Reactivity :
Chicken,Human,Mouse,Rat
Species Tested (legacy) :
mice, rat, chick, human
Initial Publication Pubmed ID :
9230312
Collections :
Cell signaling,Human,Neurodevelopment,Transcription factors
Search Keywords :
Jessell, Thomas M / Brenner-Morton, Susan, Nkx2.2 transcription factor, Chicken, MIgG2b, Mouse/Rat/Chicken/Human, NKX2-2, NK2 homeobox 2, AB_2314952 AB_531794, monoclonal
Antigen Molecular Weight :
Apparent: 28-30 kDa
Gene :
NKX2-2
Alternate Gene Name(s) :
NK2 homeobox 2
Uniprot ID :
Q9YHB7
Antibody Registry ID :
AB_2314952 AB_531794
Immunogen :
Nkx2.2-GST fusion protein expressed in E. coli.
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
No
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
12121626 21750031 23504940 11262237 27668865 29669275 31412250
Immunofluorescence Pubmed IDs :
11960707 15464286 15698615 19334287 22521513 22549777 23332756 24211383 28253550 28800946 25960414 27110779 30311912
Western Blot Pubmed IDs :
23224769 15464286
FFPE Pubmed IDs :
12121626 20417196
DSHB Growth Medium :
Iscove's
References (legacy) :
Cell 90, 169-180.; Curr. Biol. 12, 1157-1163.; Mech. Dev. 113, 169-174.; Neuroscience 128, 785-796.; Exp. Neurol. 192, 11-24.; Nat. Biotechnol. 24 (11), 1392-1401.; Dev. Dyn. 238, 1140-1149.; Dev. Biol. 343, 63-70.; Dev. Biol. 366, 279-289.; J. Biol. Chem. 287(24), 20483-20489.; Cell 152, 210-223.; J. Comp. Neurol. 521, 2088-2124.; Exp. Neurol. 251, 58-71.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US