highwire | Developmental Studies Hybridoma Bank 6H4 anti-highwire product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

highwire

catalog :

6H4 anti-highwire

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6H4 anti-highwire

reactivity :

fruit fly

application :

immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 4

Reference
Ferguson K, Long H, Cameron S, Chang W, Rao Y. The conserved Ig superfamily member Turtle mediates axonal tiling in Drosophila. J Neurosci. 2009;29:14151-9 pubmed publisher
Bazigou E, Apitz H, Johansson J, Lorén C, Hirst E, Chen P, et al. Anterograde Jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila. Cell. 2007;128:961-75 pubmed
Wu C, Wairkar Y, Collins C, DiAntonio A. Highwire function at the Drosophila neuromuscular junction: spatial, structural, and temporal requirements. J Neurosci. 2005;25:9557-66 pubmed
Wan H, DiAntonio A, Fetter R, Bergstrom K, Strauss R, Goodman C. Highwire regulates synaptic growth in Drosophila. Neuron. 2000;26:313-29 pubmed

product information

Internal ID :

3586

Name :

6H4 anti-highwire

Depositor Name :

Goodman, C.

Depositor Institution :

University of California, Berkeley

Date Deposited :

3/14/02

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

highwire

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila, others not tested

Initial Publication Pubmed ID :

10839352

Depositor Notes (Special Instructions) :

Labels presynaptic structures. Specific to HIW, no staining was observed in hiw mutants.

Collections :

Drosophila antigens,Neurodevelopment

Search Keywords :

Goodman, C., highwire, Drosophila, MIgG2b, Drosophila, hiw, CG32592, AB_528277, monoclonal, Drosophila antigens/Neurodevelopment, Immunohistochemistry/Immunofluorescence

Antigen Molecular Weight :

Predicted: 566 kDa

Gene :

hiw

Alternate Gene Name(s) :

CG32592

Uniprot ID :

Q9NB71

Antibody Registry ID :

AB_528277

Synonyms (Alternate Clone Names) :

6H4

Immunogen :

Recombinant 6× His-tagged highwire protein fragment (aa 2684–2818)

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3 x 63 Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 2684–2818

Epitope Map PubMed ID :

10839352

Recommended Applications :

Immunofluorescence,Immunohistochemistry

Immunoblotting (legacy) :

Not done

Immunohistochemistry Pubmed IDs :

10839352

Immunofluorescence Pubmed IDs :

10839352 16237161 19906964 17350579

Additional Information :

6H4 was isolated during a screen for anti-highwire mAbs [PMID: 10839352] but has since been shown to cross-react with a second unidentified presynaptic epitope distinct from Hiw in hiw mutant [PMID: 16237161].

DSHB Growth Medium :

RPMI

References (legacy) :

Neuron 26(2), 313-329.

more info or order :

Developmental Studies Hybridoma Bank product webpage