integrin alpha-2 (CD49B), human | Developmental Studies Hybridoma Bank 6F1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin alpha-2 (CD49B), human

catalog :

6F1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F1

reactivity :

human

application :

ELISA, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 24

Reference
Zhu J, Choi W, McCoy J, Negri A, Zhu J, Naini S, et al. Structure-guided design of a high-affinity platelet integrin ?IIb?3 receptor antagonist that disrupts Mg²? binding to the MIDAS. Sci Transl Med. 2012;4:125ra32 pubmed publisher
Kumar M, Girish K, Vishwanath B, Kemparaju K. The metalloprotease, NN-PF3 from Naja naja venom inhibits platelet aggregation primarily by affecting ?2?1 integrin. Ann Hematol. 2011;90:569-77 pubmed publisher
White T, Berny M, Robinson D, Yin H, Degrado W, Hanson S, et al. The leech product saratin is a potent inhibitor of platelet integrin alpha2beta1 and von Willebrand factor binding to collagen. FEBS J. 2007;274:1481-91 pubmed
Raynal N, Hamaia S, Siljander P, Maddox B, Peachey A, Fernandez R, et al. Use of synthetic peptides to locate novel integrin alpha2beta1-binding motifs in human collagen III. J Biol Chem. 2006;281:3821-31 pubmed
Penz S, Reininger A, Brandl R, Goyal P, Rabie T, Bernlochner I, et al. Human atheromatous plaques stimulate thrombus formation by activating platelet glycoprotein VI. FASEB J. 2005;19:898-909 pubmed
Cruz M, Chen J, Whitelock J, Morales L, Lopez J. The platelet glycoprotein Ib-von Willebrand factor interaction activates the collagen receptor alpha2beta1 to bind collagen: activation-dependent conformational change of the alpha2-I domain. Blood. 2005;105:1986-91 pubmed
Siljander P, Munnix I, Smethurst P, Deckmyn H, Lindhout T, Ouwehand W, et al. Platelet receptor interplay regulates collagen-induced thrombus formation in flowing human blood. Blood. 2004;103:1333-41 pubmed
Jarvis G, Atkinson B, Snell D, Watson S. Distinct roles of GPVI and integrin alpha(2)beta(1) in platelet shape change and aggregation induced by different collagens. Br J Pharmacol. 2002;137:107-17 pubmed
Monnet E, Sizaret P, Arbeille B, Fauvel Lafeve F. Different role of platelet glycoprotein GP Ia/IIa in platelet contact and activation induced by type I and type III collagens. Thromb Res. 2000;98:423-33 pubmed
Moroi M, Onitsuka I, Imaizumi T, Jung S. Involvement of activated integrin alpha2beta1 in the firm adhesion of platelets onto a surface of immobilized collagen under flow conditions. Thromb Haemost. 2000;83:769-76 pubmed
Smith C, Estavillo D, Emsley J, Bankston L, Liddington R, Cruz M. Mapping the collagen-binding site in the I domain of the glycoprotein Ia/IIa (integrin alpha(2)beta(1)). J Biol Chem. 2000;275:4205-9 pubmed
Siljander P, Lassila R. Studies of adhesion-dependent platelet activation: distinct roles for different participating receptors can be dissociated by proteolysis of collagen. Arterioscler Thromb Vasc Biol. 1999;19:3033-43 pubmed
Estavillo D, Ritchie A, Diacovo T, Cruz M. Functional analysis of a recombinant glycoprotein Ia/IIa (Integrin alpha(2)beta(1)) I domain that inhibits platelet adhesion to collagen and endothelial matrix under flow conditions. J Biol Chem. 1999;274:35921-6 pubmed
Dickeson S, Walsh J, Santoro S. Binding of the alpha 2 integrin I domain to extracellular matrix ligands: structural and mechanistic differences between collagen and laminin binding. Cell Adhes Commun. 1998;5:273-81 pubmed
Verkleij M, Morton L, Knight C, de Groot P, Barnes M, Sixma J. Simple collagen-like peptides support platelet adhesion under static but not under flow conditions: interaction via alpha2 beta1 and von Willebrand factor with specific sequences in native collagen is a requirement to resist shear forces. Blood. 1998;91:3808-16 pubmed
Jandrot Perrus M, Lagrue A, Okuma M, Bon C. Adhesion and activation of human platelets induced by convulxin involve glycoprotein VI and integrin alpha2beta1. J Biol Chem. 1997;272:27035-41 pubmed
Depraetere H, Wille C, Gansemans Y, Stanssens P, Lauwereys M, Baruch D, et al. The integrin alpha 2 beta 1 (GPIa/IIa)-I-domain inhibits platelet-collagen interaction. Thromb Haemost. 1997;77:981-5 pubmed
Dickeson S, Walsh J, Santoro S. Contributions of the I and EF hand domains to the divalent cation-dependent collagen binding activity of the alpha2beta1 integrin. J Biol Chem. 1997;272:7661-8 pubmed
Asselin J, Gibbins J, Achison M, Lee Y, Morton L, Farndale R, et al. A collagen-like peptide stimulates tyrosine phosphorylation of syk and phospholipase C gamma2 in platelets independent of the integrin alpha2beta1. Blood. 1997;89:1235-42 pubmed
Fang L, Lucero M, Kazarian T, Wei Q, Luo F, Valentino L. Effects of neuroblastoma tumor gangliosides on platelet adhesion to collagen. Clin Exp Metastasis. 1997;15:33-40 pubmed
Tuckwell D, Calderwood D, Green L, Humphries M. Integrin alpha 2 I-domain is a binding site for collagens. J Cell Sci. 1995;108 ( Pt 4):1629-37 pubmed
Kamata T, Puzon W, Takada Y. Identification of putative ligand binding sites within I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29). J Biol Chem. 1994;269:9659-63 pubmed
Coller B, Beer J, Scudder L, Steinberg M. Collagen-platelet interactions: evidence for a direct interaction of collagen with platelet GPIa/IIa and an indirect interaction with platelet GPIIb/IIIa mediated by adhesive proteins. Blood. 1989;74:182-92 pubmed
Tuszynski G, Kowalska M. Thrombospondin-induced adhesion of human platelets. J Clin Invest. 1991;87:1387-94 pubmed

product information

Internal ID :

8448

Name :

6F1

Depositor Name :

Coller, B.S.

Depositor Institution :

Rockefeller University

Date Deposited :

3/3/12

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

integrin alpha-2 (CD49B), human

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Primate

Species Tested (legacy) :

humand and primates

Initial Publication Pubmed ID :

2546619

Depositor Notes (Special Instructions) :

Inhibits binding of collagen to integrin alpha2beta1. This antibody doesn't appear to work for western blotting.

Collections :

CD antigens,Cell adhesion,Cell signaling,Extracellular matrix proteins,Human,Neurodevelopment

Search Keywords :

Coller, B.S., integrin alpha-2 (CD49B), human, Human, MIgG1, Human/Primate, ITGA2, CD49B, GPIA, BR, HPA-5, VLA-2, AB_2617392, monoclonal, Cell adhesion/Cluster Determinant antigens/Human/Cell signaling/Extracellular matrix proteins/Neurodevelopment, Immunoprecipitation/ELISA/Function Blocking/FACS

Antigen Molecular Weight :

Predicted: 129 kDa; Apparent: 154 kDa under non-reducing conditions, 166 kDa under reducing conditions.

Gene :

ITGA2

Alternate Gene Name(s) :

CD49B, GPIA, BR, HPA-5, VLA-2

Uniprot ID :

P17301

Antibody Registry ID :

AB_2617392

Immunogen :

Human platelets

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

X63-Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

I domain of alpha2 (aa 173-259)

Epitope Map PubMed ID :

7511592

Recommended Applications :

ELISA,FACS,Function Blocking,Immunoprecipitation

Immunoblotting (legacy) :

Immunoprecipitation Pubmed IDs :

2546619 7511592

ELISA Pubmed IDs :

9065422 10660584

Function Blocking Pubmed IDs :

2546619 2010551 7615681 9184414 9065422 9009104 9341142 9762468 9573018 10585478 10591685 10828482 10823276 12183336 14563646 15514009 15923400 16326707 17489103 22422993

FACS Pubmed IDs :

2546619 15514009

Pubmed IDs :

9028946 20957364

Additional Information :

6F1 recognizes alpha2 in its native conformation associated to beta1 integrin.

DSHB Growth Medium :

Iscove's

References (legacy) :

Blood 74, 182-192.; J. Clin. Invest. 87, 1387-1394.; J. Biol. Chem. 269, 9659-9663.; J. Cell Sci. 108(4), 1629-1637.; Blood 89, 1235-1242.; Thromb. Haemost. 77, 981-985.; J. Biol. Chem. 272, 7661-7668.; Clin. Exp. Metastasis 15, 33-40.; J. Biol. Chem. 272, 27035-27041.; Cell Adhes. Commun. 5, 273-281.; Blood 91, 3808-3816.; J. Biol. Chem. 274, 35921-35926.; Arterioscler. Thromb. Vasc. Biol. 19, 3033-3043.; Thromb. Res. 98, 423-433.; Thromb. Haemost. 83, 769-776.; J. Biol. Chem. 275, 4205-4209.; Blood 103, 1333-1341.; Blood 105, 1986-1991.; FASEB J. 19, 898-909.; J. Biol. Chem. 281, 3821-3831.; FEBS J. 274, 1481-1491.; Ann. Hematol. 90, 569-577.

more info or order :

Developmental Studies Hybridoma Bank product webpage