Villin-like protein quail | Developmental Studies Hybridoma Bank 6B9 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Villin-like protein quail

catalog :

6B9

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6B9

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:50; fig 6a western blot; fruit fly; 1:50; fig 4f	Groen C, Jayo A, Parsons M, Tootle T. Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture. Mol Biol Cell. 2015;26:1901-17 pubmed publisher
	Spracklen A, Kelpsch D, Chen X, Spracklen C, Tootle T. Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis. Mol Biol Cell. 2014;25:397-411 pubmed publisher
	Ogienko A, Karagodin D, Lashina V, Baiborodin S, Omelina E, Baricheva E. Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis. Cell Biol Int. 2013;37:149-59 pubmed publisher
	Groen C, Spracklen A, Fagan T, Tootle T. Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling. Mol Biol Cell. 2012;23:4567-78 pubmed publisher
	Snee M, Macdonald P. Bicaudal C and trailer hitch have similar roles in gurken mRNA localization and cytoskeletal organization. Dev Biol. 2009;328:434-44 pubmed publisher
	Borghese L, Fletcher G, Mathieu J, Atzberger A, Eades W, Cagan R, et al. Systematic analysis of the transcriptional switch inducing migration of border cells. Dev Cell. 2006;10:497-508 pubmed
	Guild G, Connelly P, Ruggiero L, Vranich K, Tilney L. Actin filament bundles in Drosophila wing hairs: hairs and bristles use different strategies for assembly. Mol Biol Cell. 2005;16:3620-31 pubmed
	Tilney L, Connelly P, Guild G. Microvilli appear to represent the first step in actin bundle formation in Drosophila bristles. J Cell Sci. 2004;117:3531-8 pubmed
	Buszczak M, Lu X, Segraves W, Chang T, Cooley L. Mutations in the midway gene disrupt a Drosophila acyl coenzyme A: diacylglycerol acyltransferase. Genetics. 2002;160:1511-8 pubmed
	Matova N, Mahajan Miklos S, Mooseker M, Cooley L. Drosophila quail, a villin-related protein, bundles actin filaments in apoptotic nurse cells. Development. 1999;126:5645-57 pubmed
	Cant K, Knowles B, Mahajan Miklos S, Heintzelman M, Cooley L. Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail. J Cell Sci. 1998;111 ( Pt 2):213-21 pubmed
	Mahajan Miklos S, Cooley L. The villin-like protein encoded by the Drosophila quail gene is required for actin bundle assembly during oogenesis. Cell. 1994;78:291-301 pubmed

product information

Internal ID :

1813

Name :

6B9

Depositor Name :

Cooley, L.

Depositor Institution :

Yale University Medical School

Depositor Website :

http://cooley.medicine.yale.edu/

Date Deposited :

7/12/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Villin-like protein quail

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8044841

Depositor Notes (Special Instructions) :

quail 6B9 (concentrated) (villin-like): Use 1:50 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or 1:50 in PBT (PBS, 0.3% Triton-X-100, and 0.5% BSA) for ovary staining. (Mahajan-Miklos and Cooley, 1994).

Collections :

Cell signaling,Cytoskeleton,Drosophila antigens

Search Keywords :

Cooley, L., Villin-like protein quail, Drosophila , MIgG1, kappa light chain, Drosophila , qua , Dmel_CG6433 , AB_528447, Monoclonal, Drosophila antigens/Cytoskeletal elements, Western blot/Immunofluorescence

Antigen Molecular Weight :

98 kDa

Gene :

qua

Alternate Gene Name(s) :

Dmel_CG6433

Uniprot ID :

Q23989

Antibody Registry ID :

AB_528447

Immunogen :

Recombinant 6XHis-Quail fusion protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

8044841 9405306 10572041 11973306 15917291 23051736 16580994 15226373 25808493 24284900 23339103 19217894

Western Blot Pubmed IDs :

8044841 9405306 10572041 23051736 25808493

Additional Information :

RRID:AB_528447 FBgn0003187.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 78, 291-301.; Cell Biol. Int. 37, 149-159.; doi: 10.1091/mbc.E12-05-0417

more info or order :

Developmental Studies Hybridoma Bank product webpage