Lim3 | Developmental Studies Hybridoma Bank 67.4E12 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Lim3

catalog :

67.4E12

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

67.4E12

reactivity :

little skate, human, mouse, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 41

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:50; loading ...; fig s1a	Luisier R, Tyzack G, Hall C, Mitchell J, Devine H, Taha D, et al. Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALS. Nat Commun. 2018;9:2010 pubmed publisher
immunohistochemistry - frozen section; little skate; fig s1e	Jung H, Baek M, D Elia K, Boisvert C, Currie P, Tay B, et al. The Ancient Origins of Neural Substrates for Land Walking. Cell. 2018;172:667-682.e15 pubmed publisher
flow cytometry; mouse; loading ...; fig 3c immunocytochemistry; mouse; 1:1000; loading ...; fig 3e	Iyer N, Huettner J, Butts J, Brown C, Sakiyama Elbert S. Generation of highly enriched V2a interneurons from mouse embryonic stem cells. Exp Neurol. 2016;277:305-316 pubmed publisher
immunohistochemistry; mouse; 1:50	Huettl R, Eckstein S, Stahl T, Petricca S, Ninkovic J, GÃ¶tz M, et al. Functional dissection of the Pax6 paired domain: Roles in neural tube patterning and peripheral nervous system development. Dev Biol. 2016;413:86-103 pubmed publisher
immunohistochemistry - frozen section; chicken; 1:1000; loading ...; tbl 1	Pirson M, Debrulle S, Clippe A, Clotman F, Knoops B. Thioredoxin-2 Modulates Neuronal Programmed Cell Death in the Embryonic Chick Spinal Cord in Basal and Target-Deprived Conditions. PLoS ONE. 2015;10:e0142280 pubmed publisher
immunohistochemistry; mouse; 1:100; fig 2	Adams K, Rousso D, Umbach J, Novitch B. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Nat Commun. 2015;6:6778 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000; fig 4	Gergics P, Brinkmeier M, Camper S. Lhx4 deficiency: increased cyclin-dependent kinase inhibitor expression and pituitary hypoplasia. Mol Endocrinol. 2015;29:597-612 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 6f	Zou M, Luo H, Xiang M. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn. 2015;244:86-97 pubmed publisher
immunohistochemistry - frozen section; chicken	Yan X, Lin J, Talabattula V, Mußmann C, Yang F, Wree A, et al. ADAM10 negatively regulates neuronal differentiation during spinal cord development. PLoS ONE. 2014;9:e84617 pubmed publisher
immunohistochemistry; chicken	Boije H, Ring H, Shirazi Fard S, Grundberg I, Nilsson M, Hallböök F. Alternative splicing of the chromodomain protein Morf4l1 pre-mRNA has implications on cell differentiation in the developing chicken retina. J Mol Neurosci. 2013;51:615-28 pubmed publisher
immunohistochemistry - paraffin section; chicken; 1:300	Kobayashi N, Homma S, Okada T, Masuda T, Sato N, Nishiyama K, et al. Elucidation of target muscle and detailed development of dorsal motor neurons in chick embryo spinal cord. J Comp Neurol. 2013;521:2987-3002 pubmed publisher
immunohistochemistry - frozen section; chicken; 1:50	Wahlin K, Hackler L, Adler R, Zack D. Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina. J Comp Neurol. 2010;518:4938-62 pubmed publisher
immunohistochemistry - frozen section; chicken; 1:50	Wahlin K, Moreira E, Huang H, Yu N, Adler R. Molecular dynamics of photoreceptor synapse formation in the developing chick retina. J Comp Neurol. 2008;506:822-37 pubmed
immunohistochemistry - frozen section; chicken; 1:50	Fischer A, Foster S, Scott M, Sherwood P. Transient expression of LIM-domain transcription factors is coincident with delayed maturation of photoreceptors in the chicken retina. J Comp Neurol. 2008;506:584-603 pubmed
	Buenaventura D, Corseri A, Emerson M. Identification of Genes With Enriched Expression in Early Developing Mouse Cone Photoreceptors. Invest Ophthalmol Vis Sci. 2019;60:2787-2799 pubmed publisher
	Osmundsen A, Keisler J, Taketo M, Davis S. Canonical WNT Signaling Regulates the Pituitary Organizer and Pituitary Gland Formation. Endocrinology. 2017;158:3339-3353 pubmed publisher
	Fu T, Towers M, Placzek M. Fgf10+ progenitors give rise to the chick hypothalamus by rostral and caudal growth and differentiation. Development. 2017;144:3278-3288 pubmed publisher
	Hunter C, Malik R, Witzmann F, Rhodes S. LHX3 interacts with inhibitor of histone acetyltransferase complex subunits LANP and TAF-1? to modulate pituitary gene regulation. PLoS ONE. 2013;8:e68898 pubmed publisher
	Su H, Wang L, Cai J, Yuan Q, Yang X, Yao X, et al. Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle. Stem Cell Res. 2013;11:529-39 pubmed publisher
	Sanyas I, Bozon M, Moret F, Castellani V. Motoneuronal Sema3C is essential for setting stereotyped motor tract positioning in limb-derived chemotropic semaphorins. Development. 2012;139:3633-43 pubmed publisher
	Roy A, Francius C, Rousso D, Seuntjens E, Debruyn J, Luxenhofer G, et al. Onecut transcription factors act upstream of Isl1 to regulate spinal motoneuron diversification. Development. 2012;139:3109-19 pubmed publisher
	Halasi G, Søviknes A, Sigurjonsson O, Glover J. Proliferation and recapitulation of developmental patterning associated with regulative regeneration of the spinal cord neural tube. Dev Biol. 2012;365:118-32 pubmed publisher
	Gutierrez C, McNally M, Canto Soler M. Cytoskeleton proteins previously considered exclusive to ganglion cells are transiently expressed by all retinal neuronal precursors. BMC Dev Biol. 2011;11:46 pubmed publisher
	Fischer A, Bongini R, Bastaki N, Sherwood P. The maturation of photoreceptors in the avian retina is stimulated by thyroid hormone. Neuroscience. 2011;178:250-60 pubmed publisher
	Hashemi H, Hurley M, Gibson A, Panova V, Tchetchelnitski V, Barr A, et al. Receptor tyrosine phosphatase PTP? is a regulator of spinal cord neurogenesis. Mol Cell Neurosci. 2011;46:469-82 pubmed publisher
	Holz A, Kollmus H, Ryge J, Niederkofler V, Dias J, Ericson J, et al. The transcription factors Nkx2.2 and Nkx2.9 play a novel role in floor plate development and commissural axon guidance. Development. 2010;137:4249-60 pubmed publisher
	Francius C, Clotman F. Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development. Neuroscience. 2010;165:116-29 pubmed publisher
	Cao X, Pfaff S, Gage F. YAP regulates neural progenitor cell number via the TEA domain transcription factor. Genes Dev. 2008;22:3320-34 pubmed publisher
	Edqvist P, Lek M, Boije H, Lindbäck S, Hallböök F. Axon-bearing and axon-less horizontal cell subtypes are generated consecutively during chick retinal development from progenitors that are sensitive to follistatin. BMC Dev Biol. 2008;8:46 pubmed publisher
	Stifani N, Freitas A, Liakhovitskaia A, Medvinsky A, Kania A, Stifani S. Suppression of interneuron programs and maintenance of selected spinal motor neuron fates by the transcription factor AML1/Runx1. Proc Natl Acad Sci U S A. 2008;105:6451-6 pubmed publisher
	Potok M, Cha K, Hunt A, Brinkmeier M, Leitges M, Kispert A, et al. WNT signaling affects gene expression in the ventral diencephalon and pituitary gland growth. Dev Dyn. 2008;237:1006-20 pubmed publisher
	Raetzman L, Cai J, Camper S. Hes1 is required for pituitary growth and melanotrope specification. Dev Biol. 2007;304:455-66 pubmed
	Davis S, Camper S. Noggin regulates Bmp4 activity during pituitary induction. Dev Biol. 2007;305:145-60 pubmed
	Cao X, Pfaff S, Gage F. A functional study of miR-124 in the developing neural tube. Genes Dev. 2007;21:531-6 pubmed
	Edqvist P, Myers S, Hallbook F. Early identification of retinal subtypes in the developing, pre-laminated chick retina using the transcription factors Prox1, Lim1, Ap2alpha, Pax6, Isl1, Isl2, Lim3 and Chx10. Eur J Histochem. 2006;50:147-54 pubmed
	Uemura O, Okada Y, Ando H, Guedj M, Higashijima S, Shimazaki T, et al. Comparative functional genomics revealed conservation and diversification of three enhancers of the isl1 gene for motor and sensory neuron-specific expression. Dev Biol. 2005;278:587-606 pubmed
	Edqvist P, Hallböök F. Newborn horizontal cells migrate bi-directionally across the neuroepithelium during retinal development. Development. 2004;131:1343-51 pubmed
	Scardigli R, Schuurmans C, Gradwohl G, Guillemot F. Crossregulation between Neurogenin2 and pathways specifying neuronal identity in the spinal cord. Neuron. 2001;31:203-17 pubmed
	Ericson J, Rashbass P, Schedl A, Brenner Morton S, Kawakami A, van Heyningen V, et al. Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling. Cell. 1997;90:169-80 pubmed
	Bohnsack J, O Shea J, Takahashi T, Brown E. Fibronectin-enhanced phagocytosis of an alternative pathway activator by human culture-derived macrophages is mediated by the C4b/C3b complement receptor (CR1). J Immunol. 1985;135:2680-6 pubmed
	Smith T, Levinson C. Quinine inhibits multiple Na+ and K+ transport mechanisms in Ehrlich ascites tumor cells. Biochim Biophys Acta. 1989;978:169-75 pubmed

product information

Internal ID :

2428

Name :

67.4E12

Depositor Name :

Jessell, T.M. / Brenner-Morton, S.

Depositor Institution :

HHMI/Columbia University

Date Deposited :

2/12/99

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Lim3

Antigen Species :

Mouse

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Chicken,Human,Mouse,Rodent

Species Tested (legacy) :

rodent, chick

Initial Publication Pubmed ID :

9230312

Collections :

Human,Transcription factors

Search Keywords :

Jessell, Lim 3, mouse, brenner-morton, MIgG1 kappa light chain, Rodent, Chicken, Mouse, Human, AB_2135805, Monoclonal, Lim-3, LIM homeobox protein 3,Plim, Lim-3,Lim3; Lhx3,Lim3b, MUSLIM3B, Lim homeobox gene 3, Chromatin Immunoprecipitation,Immunofluorescence,Immunohistochemistry,Western Blot

Antigen Molecular Weight :

44kDa

Gene :

Lim-3

Alternate Gene Name(s) :

LIM homeobox protein 3,Plim, Lim-3,Lim3; Lhx3,Lim3b, MUSLIM3B, Lim homeobox gene 3

Uniprot ID :

P50481

Antibody Registry ID :

AB_2135805

Immunogen :

full length cDNA/GST fusion

Immunogen Sequence :

full length cDNA GST Fusion

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Recommended Applications :

Chromatin Immunoprecipitation,Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

14973293 16864127 18072193 24404179 11502253 18427115 22899844 22833130 22370002 23578695 21256198 2914127 3161946 21068056 21112398 15680372

Immunofluorescence Pubmed IDs :

19800948 28938441

Immunoprecipitation Pubmed IDs :

23861948

Chromatin Immunoprecipitation Pubmed IDs :

23861948

FFPE Pubmed IDs :

28938441

Depositor Growth Medium :

20% fetal bovine serum in Iscove's DMEM with 1x pen/strep

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 90, 169-180.; Curr. Biol. 12, 1157-1163.; J. Comp. Neurol. 453, 57-70.; Cell 110, 237-249.; Neuroscience 128, 785-796.; Dev. Biol. 304, 455-466.; Dev. Biol. 305, 145-160.; Genes Dev. 21, 531-536.; Genes Dev. 22, 3320-3334.; Dev. Dyn. 237, 1006-1020.

more info or order :

Developmental Studies Hybridoma Bank product webpage