Gingipain/ P. gingivalis hemagglutinin | Developmental Studies Hybridoma Bank 61BG1.3 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Gingipain/ P. gingivalis hemagglutinin

catalog :

61BG1.3

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

61BG1.3

reactivity :

Porphyromonas gingivalis ATCC 33277, Porphyromonas gingivalis

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; Porphyromonas gingivalis; 1:50; fig 3	Chérifi H, Gogly B, Loison Robert L, Couty L, FerrÃ© F, Nassif A, et al. Comparative study of abdominal and thoracic aortic aneurysms: their pathogenesis and a gingival fibroblasts-based ex vivo treatment. Springerplus. 2015;4:231 pubmed publisher
	Ilievski V, Bhat U, Suleiman Ata S, Bauer B, Toth P, Olson S, et al. Oral application of a periodontal pathogen impacts SerpinE1 expression and pancreatic islet architecture in prediabetes. J Periodontal Res. 2017;52:1032-1041 pubmed publisher
	Ammann T, Bostanci N, Belibasakis G, Thurnheer T. Validation of a quantitative real-time PCR assay and comparison with fluorescence microscopy and selective agar plate counting for species-specific quantification of an in vitro subgingival biofilm model. J Periodontal Res. 2013;48:517-26 pubmed publisher
	Grudianov A, Kemulariia I. [Laser doppler estimation of the influence of tobacco-smoking on the blood microcirculation in the periodont at the patients with the different stages of periodontal diseases]. Stomatologiia (Mosk). 2010;89:10-4 pubmed
	Li L, Michel R, Cohen J, DeCarlo A, Kozarov E. Intracellular survival and vascular cell-to-cell transmission of Porphyromonas gingivalis. BMC Microbiol. 2008;8:26 pubmed publisher
	Dahlen G, Gmür R, Yoshino T. Phenotypes, serotypes and antibiotic susceptibility of Swedish Porphyromonas gingivalis isolates from periodontitis and periodontal abscesses. Oral Microbiol Immunol. 2007;22:80-6 pubmed
	Marcotte H, Kõll Klais P, Hultberg A, Zhao Y, Gmür R, Mandar R, et al. Expression of single-chain antibody against RgpA protease of Porphyromonas gingivalis in Lactobacillus. J Appl Microbiol. 2006;100:256-63 pubmed
	Kozarov E, Dorn B, Shelburne C, Dunn W, Progulske Fox A. Human atherosclerotic plaque contains viable invasive Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. Arterioscler Thromb Vasc Biol. 2005;25:e17-8 pubmed
	Shoji M, Ratnayake D, Shi Y, Kadowaki T, Yamamoto K, Yoshimura F, et al. Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis: cell surface polysaccharide as an anchorage for gingipains. Microbiology. 2002;148:1183-91 pubmed
	Van Tilburg M, Kozarov E, Progulske Fox A, Brady L. The effect of monoclonal antibody and route of immunization on the humoral immune response against Porphyromonas gingivalis. Oral Microbiol Immunol. 2001;16:153-62 pubmed
	Kozarov E, Miyashita N, Burks J, Cerveny K, Brown T, McArthur W, et al. Expression and immunogenicity of hemagglutinin A from Porphyromonas gingivalis in an avirulent Salmonella enterica serovar typhimurium vaccine strain. Infect Immun. 2000;68:732-9 pubmed
	Shi Y, Ratnayake D, Okamoto K, Abe N, Yamamoto K, Nakayama K. Genetic analyses of proteolysis, hemoglobin binding, and hemagglutination of Porphyromonas gingivalis. Construction of mutants with a combination of rgpA, rgpB, kgp, and hagA. J Biol Chem. 1999;274:17955-60 pubmed
	Kelly C, Booth V, Kendal H, Slaney J, Curtis M, Lehner T. The relationship between colonization and haemagglutination inhibiting and B cell epitopes of Porphyromonas gingivalis. Clin Exp Immunol. 1997;110:285-91 pubmed
	Booth V, Lehner T. Characterization of the Porphyromonas gingivalis antigen recognized by a monoclonal antibody which prevents colonization by the organism. J Periodontal Res. 1997;32:54-60 pubmed
	Booth V, Ashley F, Lehner T. Passive immunization with monoclonal antibodies against Porphyromonas gingivalis in patients with periodontitis. Infect Immun. 1996;64:422-7 pubmed
	Gmür R, Werner Felmayer G, Guggenheim B. Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis. Oral Microbiol Immunol. 1988;3:181-6 pubmed

product information

Internal ID :

6338

Name :

61BG1.3

Depositor Name :

Gmuer, R.

Depositor Institution :

Institute of Oral Biology, ZZMK, University of Zuerich

Date Deposited :

3/11/10

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Gingipain/ P. gingivalis hemagglutinin

Antigen Species :

Porphyromonas gingivalis

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Porphyromonas gingivalis

Species Tested (legacy) :

Porphyromonas gingivalis

Initial Publication Pubmed ID :

3254474

Depositor Notes (Special Instructions) :

This antibody is species specific and recognizes the hemagglutinating proteases present on all tested strains of Porphyromonas gingivalis. The hemagglutinins recognized by 61BG1.3 include RgpA (Gingipain R1; also known as prpR1 or hemagglutinin HagE), Kgp (Lys-gingipain) and HagA (Hemagglutinin A).

Collections :

Microbe antibodies

Search Keywords :

Gmuer, R., Porphyromonas gingivalis hemagglutinin, Porphyromonas gingivalis, MIgG1, Porphyromonas gingivalis, rgpA/kgp/hagA, , AB_10568665, monoclonal, Microbe antibodies, Immunohistochemistry/Immunofluorescence/ELISA/Western Blot/Function Blocking/FFPE, prprI, prpr1

Antigen Molecular Weight :

Apparent: multiple bands between 31 and 65 kDa, two bands around 14 kDa, and higher MW bands at 113 and 180 kDa.

Gene :

rgpA/kgp/hagA

Uniprot ID :

Q51838 Q51817 P59915

Antibody Registry ID :

AB_10568665

Immunogen :

Formalinized Porphyromonas gingivalis, strain W83

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

Epitope Mapped :

Yes

Epitope Location or Sequence :

beta adhesin domain of protease RI, aa 907-331 of prepro-prpR1 [Uniprot: Q51838]

Epitope Map PubMed ID :

9085243 9367414

Recommended Applications :

ELISA,FFPE,Function Blocking,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

26110102

Immunofluorescence Pubmed IDs :

23278531 28643938

Western Blot Pubmed IDs :

3254474 9085243 9367414 10364243 10639440 11932462

ELISA Pubmed IDs :

3254474 9367414

FFPE Pubmed IDs :

26110102

Function Blocking Pubmed IDs :

8550186 9085243

Pubmed IDs :

11358537 16430501

Additional Information :

*Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu).

DSHB Growth Medium :

Iscove's

References (legacy) :

Oral Microbiol. Immunol. 3, 181-186.; Oral Microbiol. Immunol. 3, 187-191.; J. Periodont. Res. 24, 113-120.; Gmuer, R. (1995). Value of new serological probes for the study of putative periodontal pathogens. Carol Stream, IL: Quintessence Publishing Co., Inc.; Infect. Immun. 64, 422-427.; Infect. Immun. 64, 2532-2539.; J. Periodontal. Res. 32, 54-60.; J. Periodont. Res. 32, 133-139.; Clin. Exp. Immunol. 110, 285-291.; J. Biol. Chem. 274, 17955-17960.; Infect. Immun. 68, 732-739.; Crit. Rev. Oral Biol. Med. 12, 192-216.; Microbiology 148, 1183-1191.; Oral Microbiol. Immunol. 19, 314-321.; Arterioscler. Thromb. Vasc. Biol. 25, e17-8. DOI=10.1161/01.ATV.0000155018.67835.1a.; J. Appl. Microbiol. 100, 256-263.; Oral Microbiol. Immunol. 22, 80-86.; BMC Microbiol 8:26.

more info or order :

Developmental Studies Hybridoma Bank product webpage