NCAM (extracellular domain), all isoforms | Developmental Studies Hybridoma Bank 5e product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

NCAM (extracellular domain), all isoforms

catalog :

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 16

Reference
Rollo B, Zhang D, Simkin J, Menheniott T, Newgreen D. Why are enteric ganglia so small? Role of differential adhesion of enteric neurons and enteric neural crest cells. F1000Res. 2015;4:113 pubmed publisher
Mao X, Zhang Y, Schwend T, Conrad G. Effects of polysialic acid on sensory innervation of the cornea. Dev Biol. 2015;398:193-205 pubmed publisher
Li X, Persad A, Monckton E, Godbout R. Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina. FEBS Lett. 2014;588:770-5 pubmed publisher
Goldstein A, Nagy N. A bird's eye view of enteric nervous system development: lessons from the avian embryo. Pediatr Res. 2008;64:326-33 pubmed publisher
Bruses J, Rutishauser U. Regulation of neural cell adhesion molecule polysialylation: evidence for nontranscriptional control and sensitivity to an intracellular pool of calcium. J Cell Biol. 1998;140:1177-86 pubmed
Agius E, Cochard P. Comparison of neurite outgrowth induced by intact and injured sciatic nerves: a confocal and functional analysis. J Neurosci. 1998;18:328-38 pubmed
Gerety M, Watanabe M. Polysialylated NCAM expression on endocardial cells of the chick primary atrial septum. Anat Rec. 1997;247:71-84 pubmed
Paz D, Alonso D, Pisano A, Casco V, Knudsen K, Peralta Soler A. Expression of isoforms of the neural cell adhesion molecule (NCAM) and polysialic acid during the development of the Bufo arenarum olfactory system. Int J Dev Biol. 1995;39:1005-13 pubmed
Rafuse V, Landmesser L. Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C. J Cell Biol. 1996;132:969-83 pubmed
Fredette B, Rutishauser U, Landmesser L. Regulation and activity-dependence of N-cadherin, NCAM isoforms, and polysialic acid on chick myotubes during development. J Cell Biol. 1993;123:1867-88 pubmed
Tavella S, Raffo P, Tacchetti C, Cancedda R, Castagnola P. N-CAM and N-cadherin expression during in vitro chondrogenesis. Exp Cell Res. 1994;215:354-62 pubmed
Keane R, Mehta P, Rose B, Honig L, Loewenstein W, Rutishauser U. Neural differentiation, NCAM-mediated adhesion, and gap junctional communication in neuroectoderm. A study in vitro. J Cell Biol. 1988;106:1307-19 pubmed
Frelinger A, Rutishauser U. Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes. J Cell Biol. 1986;103:1729-37 pubmed
Watanabe M, Frelinger A, Rutishauser U. Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes. J Cell Biol. 1986;103:1721-7 pubmed
Hall A, Nelson R, Rutishauser U. Binding properties of detergent-solubilized NCAM. J Cell Biol. 1990;110:817-24 pubmed
Acheson A, Sunshine J, Rutishauser U. NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions. J Cell Biol. 1991;114:143-53 pubmed

product information

Internal ID :

1963

Name :

Depositor Name :

Rutishauser, U.

Depositor Institution :

Memorial Sloan-Kettering Cancer Center

Date Deposited :

4/25/90

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

NCAM (extracellular domain), all isoforms

Antigen Species :

chicken

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chicken

Species Tested (legacy) :

chicken

Initial Publication Pubmed ID :

2430976

Depositor Notes (Special Instructions) :

Doesn't cross react with frog (Rana pipiens) NCAM. Attenuates NCAM-NCAM binding. Satins all NCAM positive tissues.

Collections :

Cell adhesion,Cell signaling,Extracellular matrix proteins,Neurodevelopment

Search Keywords :

Rutishauser, U., NCAM (extracellular domain), all isoforms, chicken, MIgG1, kappa light chain, chicken, NCAM1, NACM, N-CAM, AB_2058005, monoclonal, Cell adhesion/Cell signaling/Extracellular matrix proteins/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA/Function Blocking

Antigen Molecular Weight :

Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.

Gene :

NCAM1

Alternate Gene Name(s) :

NACM, N-CAM

Uniprot ID :

P13590

Antibody Registry ID :

AB_2058005

Immunogen :

Purified NCAM from embryonic chicken brain membranes (E14)

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3-X63-Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

The epitope is a polypeptide determinant located on the extracellular region of NCAM (25 kDa subtilisin heparin-binding fragment); estimated to be located around 150 aa from N-terminus.

Epitope Map PubMed ID :

2430976 2430977

Recommended Applications :

ELISA,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8986305 8276904 9412511

Immunofluorescence Pubmed IDs :

2430976 8603927 8901203 25478909 8986305 8276904 7982473 2834404 9412511

Western Blot Pubmed IDs :

2430976 2430977 8603927 2307708 25478909 24462686 8276904

Immunoprecipitation Pubmed IDs :

9490730 2307708 8603927 2050739

ELISA Pubmed IDs :

2430976 8276904

Function Blocking Pubmed IDs :

2430976

Pubmed IDs :

18636038

Additional Information :

Recognizes all isoforms. 5e has been reported to work on Bufo arenarum [PMID: 8901203] but doesn't appear to work on rana pipiens [PMID: 2307708]

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 103, 1721-1727.; J. Cell Biol. 103, 1729-1737.; J. Cell Biology 140(5), 1177-1186.; Pediatr. Res. 64, 326-333.

more info or order :

Developmental Studies Hybridoma Bank product webpage