product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Enabled
catalog :
5G2 anti-enabled
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5G2 anti-enabled
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 9
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly; 1:100; loading ...; tbl 3
Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
  • immunocytochemistry; fruit fly; 1:100; fig s3
Hicks L, Liu G, Ukken F, Lu S, Bollinger K, O Connor Giles K, et al. Depletion or over-expression of Sh3px1 results in dramatic changes in cell morphology. Biol Open. 2015;4:1448-61 pubmed publisher
Bassett A, Azzam G, Wheatley L, Tibbit C, Rajakumar T, McGowan S, et al. Understanding functional miRNA-target interactions in vivo by site-specific genome engineering. Nat Commun. 2014;5:4640 pubmed publisher
Loya C, McNeill E, Bao H, Zhang B, Van Vactor D. miR-8 controls synapse structure by repression of the actin regulator enabled. Development. 2014;141:1864-74 pubmed publisher
Spracklen A, Kelpsch D, Chen X, Spracklen C, Tootle T. Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis. Mol Biol Cell. 2014;25:397-411 pubmed publisher
Huelsmann S, Ylanne J, Brown N. Filopodia-like actin cables position nuclei in association with perinuclear actin in Drosophila nurse cells. Dev Cell. 2013;26:604-15 pubmed publisher
Becam I, Rafel N, Hong X, Cohen S, Milan M. Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing. Development. 2011;138:3781-9 pubmed publisher
Song J, Kannan R, Merdes G, Singh J, Mlodzik M, Giniger E. Disabled is a bona fide component of the Abl signaling network. Development. 2010;137:3719-27 pubmed publisher
Bashaw G, Kidd T, Murray D, Pawson T, Goodman C. Repulsive axon guidance: Abelson and Enabled play opposing roles downstream of the roundabout receptor. Cell. 2000;101:703-15 pubmed
product information
Internal ID :
3574
Name :
5G2 anti-enabled
Depositor Name :
Goodman, C.
Depositor Institution :
University of California, Berkeley
Date Deposited :
3/14/02
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Enabled
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila, others not tested
Initial Publication Pubmed ID :
10892742
Collections :
Cell signaling,Cytoskeleton,Drosophila antigens,Neurodevelopment
Search Keywords :
Goodman, Corey, Enabled, Drosophila, MIgG1, Drosophila, Ena/enabled, Ena/ ENA/VASP/ enb/Enb/ l(2)02029/Vasp/VASP, AB_528220, monoclonal
Antigen Molecular Weight :
30 kDa
Gene :
Ena/enabled
Alternate Gene Name(s) :
Ena/ ENA/VASP/ enb/Enb/ l(2)02029/Vasp/VASP
Uniprot ID :
Q8T4F7
Antibody Registry ID :
AB_528220
Immunogen :
Enabled (a.a. 105-370) fusion protein tagged with 6X-HIS.
Immunogen Sequence :
aa105-370
Clonality :
Monoclonal
Myeloma Strain :
P3 x 63 Ag8.653
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunofluorescence Pubmed IDs :
25135198 24284900 20940230 21795284
Western Blot Pubmed IDs :
24718988 24284900 24091012 20940230
Immunoprecipitation Pubmed IDs :
10892742
DSHB Growth Medium :
RPMI
References (legacy) :
Cell 101, 703-715.; Mech. 4(1), 126-134.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US