allatostatin (Ast7) | Developmental Studies Hybridoma Bank 5F10 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

allatostatin (Ast7)

catalog :

5F10

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5F10

reactivity :

hermit crabs, American cockroach, fruit fly

application :

ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:50; loading ...; fig 1i	Ni J, Gurav A, Liu W, Ogunmowo T, Hackbart H, Elsheikh A, et al. Differential regulation of the Drosophila sleep homeostat by circadian and arousal inputs. elife. 2019;8: pubmed publisher
immunohistochemistry - paraffin section; American cockroach; 1:100 immunohistochemistry - paraffin section; hermit crabs; 1:100	Wolff G, Harzsch S, Hansson B, Brown S, STRAUSFELD N. Neuronal organization of the hemiellipsoid body of the land hermit crab, Coenobita clypeatus: correspondence with the mushroom body ground pattern. J Comp Neurol. 2012;520:2824-46 pubmed publisher
	Chen J, Xu N, Wang C, Huang P, Huang H, Jin Z, et al. Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells. Nat Cell Biol. 2018;20:152-161 pubmed publisher
	Maza F, Sztarker J, Shkedy A, Peszano V, Locatelli F, Delorenzi A. Context-dependent memory traces in the crab's mushroom bodies: Functional support for a common origin of high-order memory centers. Proc Natl Acad Sci U S A. 2016;113:E7957-E7965 pubmed
	Bento C, Ashkenazi A, Jimenez Sanchez M, Rubinsztein D. The Parkinson's disease-associated genes ATP13A2 and SYT11 regulate autophagy via a common pathway. Nat Commun. 2016;7:11803 pubmed publisher
	Beehler Evans R, Micchelli C. Generation of enteroendocrine cell diversity in midgut stem cell lineages. Development. 2015;142:654-64 pubmed publisher
	Fraguas S, Barberán S, Ibarra B, Stöger L, Cebria F. Regeneration of neuronal cell types in Schmidtea mediterranea: an immunohistochemical and expression study. Int J Dev Biol. 2012;56:143-53 pubmed publisher
	Szabo T, Chen R, Goeritz M, Maloney R, Tang L, Li L, et al. Distribution and physiological effects of B-type allatostatins (myoinhibitory peptides, MIPs) in the stomatogastric nervous system of the crab Cancer borealis. J Comp Neurol. 2011;519:2658-76 pubmed publisher
	Wilson C, Christie A. Distribution of C-type allatostatin (C-AST)-like immunoreactivity in the central nervous system of the copepod Calanus finmarchicus. Gen Comp Endocrinol. 2010;167:252-60 pubmed publisher
	Stay B, Zera A. Morph-specific diurnal variation in allatostatin immunostaining in the corpora allata of Gryllus firmus: implications for the regulation of a morph-specific circadian rhythm for JH biosynthetic rate. J Insect Physiol. 2010;56:266-70 pubmed publisher
	Elliott K, Chan K, Stay B. Evidence for a Phe-Gly-Leu-amide-like allatostatin in the beetle Tenebrio molitor. Peptides. 2010;31:402-7 pubmed publisher
	Cebria F. Organization of the nervous system in the model planarian Schmidtea mediterranea: an immunocytochemical study. Neurosci Res. 2008;61:375-84 pubmed publisher
	Sullivan J, Benton J, Sandeman D, Beltz B. Adult neurogenesis: a common strategy across diverse species. J Comp Neurol. 2007;500:574-84 pubmed
	Skinner J, Fairbairn S, Woodhead A, Bendena W, Stay B. Allatostatin in hemocytes of the cockroach Diploptera punctata. Cell Tissue Res. 1997;290:119-28 pubmed
	Yoon J, Stay B. Immunocytochemical localization of Diploptera punctata allatostatin-like peptide in Drosophila melanogaster. J Comp Neurol. 1995;363:475-88 pubmed
	Neuhüser T, Sorge D, Stay B, Hoffmann K. Responsiveness of the adult cricket (Gryllus bimaculatus and Acheta domesticus) retrocerebral complex to allatostatin-1 from a cockroach, Diploptera punctata. J Comp Physiol B. 1994;164:23-31 pubmed
	Stay B, Chan K, Woodhead A. Allatostatin-immunoreactive neurons projecting to the corpora allata of adult Diploptera punctata. Cell Tissue Res. 1992;270:15-23 pubmed

product information

Internal ID :

2338

Name :

5F10

Depositor Name :

Stay, B.

Depositor Institution :

University of Iowa

Date Deposited :

7/10/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

allatostatin (Ast7)

Antigen Species :

Cockroach

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Cockroach,Copepod,Crab,Drosophila melanogaster,Earthworm,Insect,Invertebrate,Schmidtea mediterranea,Snail

Species Tested (legacy) :

many insect species, many invertebrates; snail, crab, earthworm

Initial Publication Pubmed ID :

1423517

Depositor Notes (Special Instructions) :

Works on many insect species and many invertebrates. Some cross reaction with leu-callatostatin 1. Stains neural tissue, gut endocrine cells and ovary.

Collections :

Cell signaling,Drosophila antigens,Neurodevelopment

Search Keywords :

Stay, B., allatostatin (Ast7), Cockroach, MIgG1, Cockroach/insects/invertebrate/snail/copepod/crab/earthworm/drosophila melanogaster/Schmidtea mediterranea, , , AB_528076, monoclonal, Cell signaling/Neurodevelopment/Drosophila antigens, Immunohistochemistry/Immunofluorescence/ELISA

Antigen Molecular Weight :

Predicted: 39 kDa (propeptide), 1.3 kDa (ATS7)

Uniprot ID :

P12764

Antibody Registry ID :

AB_528076

Immunogen :

synthetic AST7 (APSGAQRLYGFGL), N-terminal coupled to BSA

Immunogen Sequence :

peptide

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

likely YGFGL

Epitope Map PubMed ID :

1423517 8847412

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry

Immunohistochemistry Pubmed IDs :

1423517 8847412 9377632 8014257

Immunofluorescence Pubmed IDs :

8847412 18499291 9377632 25670792 19793542 27856766 22451002 19913023 20338176 29335529

ELISA Pubmed IDs :

1423517 8847412

Additional Information :

Stains type A allatostatins.

DSHB Growth Medium :

RPMI

References (legacy) :

Cell Tissue Res. 270, 15-23.; J. Comp. Neurol. 363, 475-488.

more info or order :

Developmental Studies Hybridoma Bank product webpage