sonic hedgehog | Developmental Studies Hybridoma Bank 5E1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

sonic hedgehog

catalog :

5E1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5E1

reactivity :

Gallotia galloti, common quail, human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, FACS, immunohistochemistry - paraffin section, immunohistochemistry knockout validation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 46

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; 1:20; loading ...; fig s8b immunohistochemistry - paraffin section; mouse; 1:20; loading ...; fig 6b	He H, Huang M, Sun S, Wu Y, Lin X. Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development. PLoS Genet. 2017;13:e1006992 pubmed publisher
immunohistochemistry knockout validation; common quail; loading ...; fig 6c	Matsubara Y, Nakano M, Kawamura K, Tsudzuki M, Funahashi J, Agata K, et al. Inactivation of Sonic Hedgehog Signaling and Polydactyly in Limbs of Hereditary Multiple Malformation, a Novel Type of Talpid Mutant. Front Cell Dev Biol. 2016;4:149 pubmed publisher
immunohistochemistry; chicken; 1:200; fig s2	Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
western blot; human; 1:500; fig 4b	Coffman L, Choi Y, McLean K, Allen B, di Magliano M, Buckanovich R. Human carcinoma-associated mesenchymal stem cells promote ovarian cancer chemotherapy resistance via a BMP4/HH signaling loop. Oncotarget. 2016;7:6916-32 pubmed publisher
immunocytochemistry; mouse; 1:10; fig 3	Alfaro A, Roberts B, Kwong L, Bijlsma M, Roelink H. Ptch2 mediates the Shh response in Ptch1-/- cells. Development. 2014;141:3331-9 pubmed publisher
immunohistochemistry - paraffin section; Gallotia galloti; 1:2	Romero Alemán M, Monzon Mayor M, Santos E, Lang D, Yanes C. Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny. J Comp Neurol. 2012;520:2163-84 pubmed publisher
	Yang C, Li X, Li Q, Li H, Qiao L, Guo Z, et al. Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development. J Mol Neurosci. 2018;64:287-299 pubmed publisher
	AvilÃ©s E, Stoeckli E. Canonical wnt signaling is required for commissural axon guidance. Dev Neurobiol. 2016;76:190-208 pubmed publisher
	Furmanski A, Saldana J, Ono M, Sahni H, Paschalidis N, D Acquisto F, et al. Tissue-derived hedgehog proteins modulate Th differentiation and disease. J Immunol. 2013;190:2641-9 pubmed publisher
	Voronova A, Coyne E, Al Madhoun A, Fair J, Bosiljcic N, St Louis C, et al. Hedgehog signaling regulates MyoD expression and activity. J Biol Chem. 2013;288:4389-404 pubmed publisher
	Boije H, Harun Or Rashid M, Lee Y, Imsland F, Bruneau N, Vieaud A, et al. Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation. PLoS ONE. 2012;7:e50890 pubmed publisher
	Wang W, Lian N, Ma Y, Li L, Gallant R, Elefteriou F, et al. Chondrocytic Atf4 regulates osteoblast differentiation and function via Ihh. Development. 2012;139:601-11 pubmed publisher
	Gerhart J, Scheinfeld V, Milito T, Pfautz J, Neely C, Fisher Vance D, et al. Myo/Nog cell regulation of bone morphogenetic protein signaling in the blastocyst is essential for normal morphogenesis and striated muscle lineage specification. Dev Biol. 2011;359:12-25 pubmed publisher
	Wilson N, Stoeckli E. Cell type specific, traceable gene silencing for functional gene analysis during vertebrate neural development. Nucleic Acids Res. 2011;39:e133 pubmed publisher
	Ohlig S, Farshi P, Pickhinke U, van den Boom J, Höing S, Jakuschev S, et al. Sonic hedgehog shedding results in functional activation of the solubilized protein. Dev Cell. 2011;20:764-74 pubmed publisher
	Farshi P, Ohlig S, Pickhinke U, Höing S, Jochmann K, Lawrence R, et al. Dual roles of the Cardin-Weintraub motif in multimeric Sonic hedgehog. J Biol Chem. 2011;286:23608-19 pubmed publisher
	Cho S, Kwak S, Woolley T, Lee M, Kim E, Baker R, et al. Interactions between Shh, Sostdc1 and Wnt signaling and a new feedback loop for spatial patterning of the teeth. Development. 2011;138:1807-16 pubmed publisher
	Nagy N, Oláh I. Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of Fabricius. Development. 2010;137:3019-23 pubmed publisher
	Maun H, Wen X, Lingel A, De Sauvage F, Lazarus R, Scales S, et al. Hedgehog pathway antagonist 5E1 binds hedgehog at the pseudo-active site. J Biol Chem. 2010;285:26570-80 pubmed publisher
	Favaro R, Valotta M, Ferri A, Latorre E, Mariani J, Giachino C, et al. Hippocampal development and neural stem cell maintenance require Sox2-dependent regulation of Shh. Nat Neurosci. 2009;12:1248-56 pubmed publisher
	Kobune M, Takimoto R, Murase K, Iyama S, Sato T, Kikuchi S, et al. Drug resistance is dramatically restored by hedgehog inhibitors in CD34+ leukemic cells. Cancer Sci. 2009;100:948-55 pubmed publisher
	Ohyama K, Das R, Placzek M. Temporal progression of hypothalamic patterning by a dual action of BMP. Development. 2008;135:3325-31 pubmed publisher
	Yauch R, Gould S, Scales S, Tang T, Tian H, Ahn C, et al. A paracrine requirement for hedgehog signalling in cancer. Nature. 2008;455:406-10 pubmed publisher
	Reeber S, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson J, et al. Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord. J Neurosci. 2008;28:8698-708 pubmed publisher
	Angot E, Loulier K, Nguyen Ba Charvet K, Gadeau A, Ruat M, Traiffort E. Chemoattractive activity of sonic hedgehog in the adult subventricular zone modulates the number of neural precursors reaching the olfactory bulb. Stem Cells. 2008;26:2311-20 pubmed publisher
	Zavros Y, Orr M, Xiao C, Malinowska D. Sonic hedgehog is associated with H+-K+-ATPase-containing membranes in gastric parietal cells and secreted with histamine stimulation. Am J Physiol Gastrointest Liver Physiol. 2008;295:G99-G111 pubmed publisher
	Peacock C, Wang Q, Gesell G, Corcoran Schwartz I, Jones E, Kim J, et al. Hedgehog signaling maintains a tumor stem cell compartment in multiple myeloma. Proc Natl Acad Sci U S A. 2007;104:4048-53 pubmed
	Chan V, Chau S, Tian L, Chen Y, Kwong S, Quackenbush J, et al. Sonic hedgehog promotes CD4+ T lymphocyte proliferation and modulates the expression of a subset of CD28-targeted genes. Int Immunol. 2006;18:1627-36 pubmed
	Hochman E, Castiel A, Jacob Hirsch J, Amariglio N, Izraeli S. Molecular pathways regulating pro-migratory effects of Hedgehog signaling. J Biol Chem. 2006;281:33860-70 pubmed
	Riobo N, Lu K, Ai X, Haines G, Emerson C. Phosphoinositide 3-kinase and Akt are essential for Sonic Hedgehog signaling. Proc Natl Acad Sci U S A. 2006;103:4505-10 pubmed
	Yu L, Gu S, Alappat S, Song Y, Yan M, Zhang X, et al. Shox2-deficient mice exhibit a rare type of incomplete clefting of the secondary palate. Development. 2005;132:4397-406 pubmed
	Zeltser L. Shh-dependent formation of the ZLI is opposed by signals from the dorsal diencephalon. Development. 2005;132:2023-33 pubmed
	Bok J, Bronner Fraser M, Wu D. Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear. Development. 2005;132:2115-24 pubmed
	Madison B, Braunstein K, Kuizon E, Portman K, Qiao X, Gumucio D. Epithelial hedgehog signals pattern the intestinal crypt-villus axis. Development. 2005;132:279-89 pubmed
	Ju M, Aroca P, Luo J, Puelles L, Redies C. Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate. Neuroscience. 2004;128:785-96 pubmed
	Akazawa C, Tsuzuki H, Nakamura Y, Sasaki Y, Ohsaki K, Nakamura S, et al. The upregulated expression of sonic hedgehog in motor neurons after rat facial nerve axotomy. J Neurosci. 2004;24:7923-30 pubmed
	Sanchez P, Hernandez A, Stecca B, Kahler A, DeGueme A, Barrett A, et al. Inhibition of prostate cancer proliferation by interference with SONIC HEDGEHOG-GLI1 signaling. Proc Natl Acad Sci U S A. 2004;101:12561-6 pubmed
	Kobune M, Ito Y, Kawano Y, Sasaki K, Uchida H, Nakamura K, et al. Indian hedgehog gene transfer augments hematopoietic support of human stromal cells including NOD/SCID-beta2m-/- repopulating cells. Blood. 2004;104:1002-9 pubmed
	Berman D, Karhadkar S, Maitra A, Montes De Oca R, Gerstenblith M, Briggs K, et al. Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours. Nature. 2003;425:846-51 pubmed
	Zeng X, Goetz J, Suber L, Scott W, Schreiner C, Robbins D. A freely diffusible form of Sonic hedgehog mediates long-range signalling. Nature. 2001;411:716-20 pubmed
	Zhang X, Yang X. Temporal and spatial effects of Sonic hedgehog signaling in chick eye morphogenesis. Dev Biol. 2001;233:271-90 pubmed
	Outram S, Varas A, Pepicelli C, Crompton T. Hedgehog signaling regulates differentiation from double-negative to double-positive thymocyte. Immunity. 2000;13:187-97 pubmed
	Wallace V, Raff M. A role for Sonic hedgehog in axon-to-astrocyte signalling in the rodent optic nerve. Development. 1999;126:2901-9 pubmed
	Wallace V. Purkinje-cell-derived Sonic hedgehog regulates granule neuron precursor cell proliferation in the developing mouse cerebellum. Curr Biol. 1999;9:445-8 pubmed
	Ericson J, Morton S, Kawakami A, Roelink H, Jessell T. Two critical periods of Sonic Hedgehog signaling required for the specification of motor neuron identity. Cell. 1996;87:661-73 pubmed
	Mittelman A, Bertram J, Henry D, Snyder H, Messerschmidt G, Ciavarella D, et al. Treatment of patients with HIV thrombocytopenia and hemolytic uremic syndrome with protein A (Prosorba column) immunoadsorption. Semin Hematol. 1989;26:15-8 pubmed

product information

Internal ID :

2410

Name :

5E1

Depositor Name :

Jessell, T.M. / Brenner-Morton, S.

Depositor Institution :

HHMI/Columbia University

Date Deposited :

12/3/96

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

sonic hedgehog

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chicken,Human,Lizard,Mouse,Quail,Rabbit,Rat

Species Tested (legacy) :

chick, rat, mouse, human

Initial Publication Pubmed ID :

8929535

Depositor Notes (Special Instructions) :

Ligand for the Patched receptor

Collections :

Cell signaling,Extracellular matrix proteins,Human,Neurodevelopment

Search Keywords :

Jessell, sonic hedgehog, mouse, brenner, SHH-N, Monoclonal, rat, MIgG1, Chicken, Rat, Mouse, Human,Quail, Rabbit, AB_2188307

Antigen Molecular Weight :

20 kDa SHH-N protein

Gene :

Shh

Alternate Gene Name(s) :

sonic hedgehog

Uniprot ID :

Q63673

Antibody Registry ID :

AB_2188307

Synonyms (Alternate Clone Names) :

m5E1, alpha-SHH

Immunogen :

synthetic peptide

Immunogen Sequence :

N-terminal SSH fragment

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa. 157-178

Epitope Map PubMed ID :

20504762 21572042 21664575

Recommended Applications :

FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

15464286 20702559 20504762 21824915 15590741 18753371 19734891

Immunofluorescence Pubmed IDs :

21884693 18483183 15356205 16537363 18787065 18787065 29285739 29285739 27093082

Western Blot Pubmed IDs :

16943197 15105288

Immunoprecipitation Pubmed IDs :

28859094

Function Blocking Pubmed IDs :

8929535 10981962 11336495 15314219 23266826 20504762 10357934 10226030 14520411 18617686 18754008 11395778 2658095 21664575 21572042 21447550 15788455 16943197 17005629 22190639 19245435 17360475 15788458 15105288 16141225 23408837

TRAP Pubmed IDs :

20504762 18483183

Depositor Growth Medium :

20% fetal bovine serum in Iscove's DMEM with 1x pen/strep

Additional Information :

Also recognizes IHH (Indian Hedghog) PMID: 8662546 . The functionally blocked receptor is ptc. Does not bind to ShhC25S protein. For experiments involving live cells and tissue, supernatant of 5E1 is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 87, 661-673.; Immunity 13, 187-197.; Dev. Biol. 233, 271-290.; Neuroscience 128, 785-796.; PNAS 101(34), 12561-12566.; Development 137, 3019-3023.; Dev. Biol. 359, 12-25.; J. Biol. Chem. 288(6), 4389-4404.

more info or order :

Developmental Studies Hybridoma Bank product webpage