Cadherin, E- | Developmental Studies Hybridoma Bank 5D3 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Cadherin, E-

catalog :

5D3

clonality :

monoclonal

host :

house mouse

conjugate :

nonconjugated

clone name :

5D3

reactivity :

Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; Xenopus laevis; 1:100; loading ...; fig 3b	Balashova O, Visina O, Borodinsky L. Folate receptor 1 is necessary for neural plate cell apical constriction during Xenopus neural tube formation. Development. 2017;144:1518-1530 pubmed publisher
	Zhang Z, Rankin S, Zorn A. Syndecan4 coordinates Wnt/JNK and BMP signaling to regulate foregut progenitor development. Dev Biol. 2016;416:187-199 pubmed publisher
	Scarpa E, SzabÃ³ A, Bibonne A, Theveneau E, Parsons M, Mayor R. Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces. Dev Cell. 2015;34:421-34 pubmed publisher
	Breznau E, Semack A, Higashi T, Miller A. MgcRacGAP restricts active RhoA at the cytokinetic furrow and both RhoA and Rac1 at cell-cell junctions in epithelial cells. Mol Biol Cell. 2015;26:2439-55 pubmed publisher
	König A, Shcherbata H. Visualization of adult stem cells within their niches using the Drosophila germline as a model system. Methods Mol Biol. 2013;1035:25-33 pubmed publisher
	Theveneau E, Steventon B, Scarpa E, Garcia S, Trepat X, Streit A, et al. Chase-and-run between adjacent cell populations promotes directional collective migration. Nat Cell Biol. 2013;15:763-72 pubmed publisher
	Harata A, Matsuzaki T, Nishikawa A, Ihara S. The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-? signaling. In Vitro Cell Dev Biol Anim. 2013;49:220-9 pubmed publisher
	Burns J, Collado M, Oliver E, Corwin J. Specializations of intercellular junctions are associated with the presence and absence of hair cell regeneration in ears from six vertebrate classes. J Comp Neurol. 2013;521:1430-48 pubmed publisher
	Ponferrada V, Fan J, Vallance J, Hu S, Mamedova A, Rankin S, et al. CRIM1 complexes with ß-catenin and cadherins, stabilizes cell-cell junctions and is critical for neural morphogenesis. PLoS ONE. 2012;7:e32635 pubmed publisher
	Quigley I, Stubbs J, Kintner C. Specification of ion transport cells in the Xenopus larval skin. Development. 2011;138:705-14 pubmed publisher
	Stubbs J, Davidson L, Keller R, Kintner C. Radial intercalation of ciliated cells during Xenopus skin development. Development. 2006;133:2507-15 pubmed
	Kitamoto J, Fukui A, Asashima M. Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation. Adv Space Res. 2005;35:1654-61 pubmed
	Teague W, Jayanthi N, Lear P, Johnson P. Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick-quail chimera model. Pediatr Surg Int. 2005;21:138-42 pubmed
	Levine E, Lee C, Kintner C, Gumbiner B. Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant. Development. 1994;120:901-9 pubmed
	Choi Y, Gumbiner B. Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm. J Cell Biol. 1989;108:2449-58 pubmed
	Levi G, Gumbiner B, Thiery J. The distribution of E-cadherin during Xenopus laevis development. Development. 1991;111:159-69 pubmed

product information

Internal ID :

3789

Name :

5D3

Depositor Name :

Gumbiner, B.M.

Depositor Institution :

University of Virginia

Date Deposited :

5/7/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Cadherin, E-

Antigen Species :

Xenopus

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b, kappa light chain

Positive Tested Species Reactivity :

Drosophila,Xenopus

Species Tested (legacy) :

Xenopus, other amphibia not tested

Initial Publication Pubmed ID :

2472408

Depositor Notes (Special Instructions) :

Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.

Collections :

Cardiac development,Cell adhesion,Cell signaling,Cytoskeleton,Drosophila antigens,Neurodevelopment,Xenopus

Search Keywords :

Gumbiner, Barry M., Cadherin, E-, Xenopus, MIgG2b, Xenopus, Cdh1, arc-1/cd324/cdhe/ecad/lcam/uvo, AB_528116, monoclonal

Gene :

Cdh1

Alternate Gene Name(s) :

arc-1/cd324/cdhe/ecad/lcam/uvo

Uniprot ID :

P30944

Antibody Registry ID :

AB_528116

Immunogen :

Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.

Immunogen Sequence :

partial protein

Clonality :

Monoclonal

Myeloma Strain :

sp-2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

Extracellular domain

Epitope Map PubMed ID :

2472408

Recommended Applications :

FFPE,Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

16728476 23124808 21266406 2472408 2015791 16175731 23770678 26235046 23959979

Western Blot Pubmed IDs :

2472408 15578193 7600966

Immunoprecipitation Pubmed IDs :

2472408 15578193

FFPE Pubmed IDs :

23435857

Function Blocking Pubmed IDs :

2472408 26235046

Additional Information :

5D3 binds both the - and + (Ca++) conformations of E-cadherin

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 108, 2449-2458.

more info or order :

Developmental Studies Hybridoma Bank product webpage