CD44 | Developmental Studies Hybridoma Bank 5D2-27 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

CD44

catalog :

5D2-27

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

5D2-27

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, western blot knockout validation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig 2b	Freeman S, Vega A, Riedl M, Collins R, Ostrowski P, Woods E, et al. Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement. Cell. 2018;172:305-317.e10 pubmed publisher
	Caceres P, Gravotta D, Zager P, Dephoure N, Rodriguez Boulan E. Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins. Proc Natl Acad Sci U S A. 2019;116:11796-11805 pubmed publisher
	Lewis J, Schwartz M. Mapping in vivo associations of cytoplasmic proteins with integrin beta 1 cytoplasmic domain mutants. Mol Biol Cell. 1995;6:151-60 pubmed
	Hughes E, August J. Characterization of plasma membrane proteins identified by monoclonal antibodies. J Biol Chem. 1981;256:664-71 pubmed
	Hughes E, Mengod G, August J. Murine cell surface glycoproteins. Characterization of a major component of 80,000 daltons as a polymorphic differentiation antigen of mesenchymal cells. J Biol Chem. 1981;256:7023-7 pubmed
	Murphy T, Decker G, August J. Glycoproteins of coated pits, cell junctions, and the entire cell surface revealed by monoclonal antibodies and immunoelectron microscopy. J Cell Biol. 1983;97:533-41 pubmed
	Jacobson K, O DELL D, August J. Lateral diffusion of an 80,000-dalton glycoprotein in the plasma membrane of murine fibroblasts: relationships to cell structure and function. J Cell Biol. 1984;99:1624-33 pubmed
	Jacobson K, O DELL D, Holifield B, Murphy T, August J. Redistribution of a major cell surface glycoprotein during cell movement. J Cell Biol. 1984;99:1613-23 pubmed

product information

Internal ID :

468

Name :

5D2-27

Depositor Name :

August, J.T.

Depositor Institution :

Johns Hopkins University School of Medicine

Depositor Website :

https://www.hopkinsmedicine.org/pharmacology_molecular_sciences/faculty/bios/august.html

Date Deposited :

8/1/86

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

CD44

Antigen Species :

Mouse

Host Species :

rat

Isotype :

RIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Mouse

Species Tested (legacy) :

specific to mouse

Initial Publication Pubmed ID :

7451466

Depositor Notes (Special Instructions) :

This antigen was originally called Pgp-1 (Ly-24) lymphocyte cell adhesion glycoprotein and the antibody was identified as AMF-15. The 5D2-27 mAb is specific for mouse CD44 only.

Collections :

CD antigens,Cell adhesion,Cell markers,Cell signaling,Extracellular matrix proteins

Search Keywords :

August, J.T., CD44, Mouse, RIgG2a, Mouse, CD44, Ly-24, AB_2076440, Monoclonal , Cell markers/Cell adhesion/Cluster Determinant antigens, Immunoprecipitation/Western blot/ELISA/FACS/Immunofluorescence/Immunohistochemistry

Antigen Molecular Weight :

80kDa

Gene :

CD44

Alternate Gene Name(s) :

Ly-24

Uniprot ID :

P15379

Antibody Registry ID :

AB_2076440

Synonyms (Alternate Clone Names) :

CD44

Immunogen :

NIH 3T3 fibroblast plasma membranes

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3-NSI/1-Ag4-1

Epitope Mapped :

Recommended Applications :

ELISA,FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

6411735

Immunofluorescence Pubmed IDs :

6386823 6386824

Immunoprecipitation Pubmed IDs :

7451466 6386823 6787057

ELISA Pubmed IDs :

7451466

FACS Pubmed IDs :

7540435 6787057

Additional Information :

RRID:AB_2076440

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Biol. Chem. 256, 664-671.; J. Biol. Chem. 256, 7023-7027.; Arch. Biochem. Biophys. 209, 718-722.; Proc. Natl. Acad. Sci. USA 79, 1926-1929.; J. Biol. Chem. 258, 1014-1021.; J. Cell Biol. 97, 533-541.; J. Cell Biol. 99, 1613-1623.; J. Cell Biol. 99, 1624-1633.; J. Tiss. Cult. Meth. 14, 31-36.

more info or order :

Developmental Studies Hybridoma Bank product webpage