Neural Cell Adhesion molecular 1; NCAM | Developmental Studies Hybridoma Bank 5B8 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Neural Cell Adhesion molecular 1; NCAM

catalog :

5B8

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5B8

reactivity :

mouse, rat, dog, cat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1c	Yang Z, Zimmerman S, Tsunezumi J, Braitsch C, Trent C, Bryant D, et al. Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016;418:66-74 pubmed publisher
immunohistochemistry - paraffin section; cat; fig 3 western blot; cat; fig 1	Kaese M, Galuska C, Simon P, Braun B, Cabrera Fuentes H, Middendorff R, et al. Polysialylation takes place in granulosa cells during apoptotic processes of atretic tertiary follicles. FEBS J. 2015;282:4595-606 pubmed publisher
	Litbarg N, Vujicic S, Setty S, Sethupathi P, Dunea G, Arruda J, et al. A novel model of surgical injury in adult rat kidney: a "pouch model". Sci Rep. 2013;3:2890 pubmed publisher
	Páez P, Batiz L, Roales Buján R, Rodríguez Pérez L, Rodriguez S, Jiménez A, et al. Patterned neuropathologic events occurring in hyh congenital hydrocephalic mutant mice. J Neuropathol Exp Neurol. 2007;66:1082-92 pubmed
	Sytnyk V, Leshchyns ka I, Delling M, Dityateva G, Dityatev A, Schachner M. Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts. J Cell Biol. 2002;159:649-61 pubmed

product information

Internal ID :

2629

Name :

5B8

Depositor Name :

Jessell, T.M. / Dodd, J. / Brenner-Morton, S.

Depositor Institution :

Columbia University

Depositor Website :

http://sklad.cumc.columbia.edu/jessell/

Date Deposited :

3/15/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Neural Cell Adhesion molecular 1; NCAM

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine,Mouse,Rat

Species Tested (legacy) :

rat, mouse

Initial Publication Pubmed ID :

3272160

Depositor Notes (Special Instructions) :

This antibody has also been used for isolation of neuronal and glial precursors by immunopanning (PMID: 16631155). 5B8 also recognizes human NCAM in IMR-32 cells (PMID: 12023285).

Collections :

Cardiac development,Cell adhesion,Cell markers,Cell signaling,Cytoskeleton,Extracellular matrix proteins,Neurodevelopment,Skeletal muscle,Stem cell

Search Keywords :

Jessell, T.M. / Dodd, J. / Brenner-Morton, S., Neural Cell Adhesion molecular 1; NCAM, Rat, MIgG1, kappa light chain, Rat/Mouse/Canine, Ncam1, 94.6 kDa, AB_528393, Monoclonal , Neurodevelopment/Cell markers/Cell adhesion, Western blot/Immunopreicpitation/Immunohistochemistry/immunofluorescnce/ELISA/FFPE

Antigen Molecular Weight :

140 kDa /180 kDa

Gene :

Ncam1

Alternate Gene Name(s) :

94.6 kDa

Uniprot ID :

P13596

Antibody Registry ID :

AB_528393

Immunogen :

Embryonic spinal cord membranes

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Cytoplasmic domain

Epitope Map PubMed ID :

3272160

Recommended Applications :

ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

26511275 25754206 24100472 23487309 19580868 22057188 19578404 18184769 12438412 11784049 9486822 8682932 7528221 7515726 1312139 3272160

Immunofluorescence Pubmed IDs :

28860115 26516208 26516208 25754206 25811887 24133283 24100472 21718676 19041873 18090917 14757642 12743109 11784049 7528221

Western Blot Pubmed IDs :

24133283 20702708 19041873 18184769 12743109 12023285 3272160

Immunoprecipitation Pubmed IDs :

24133283 20702708

ELISA Pubmed IDs :

27608812 20702708

FFPE Pubmed IDs :

26511275 24285501 24100472 23487309 21718676 19580868 9486822 8682932

Additional Information :

RRID: AB_528393

DSHB Growth Medium :

Iscove's

References (legacy) :

Neuron 1, 105-116.; J. Neurosci. 12(3), 930-945.; Dev. Dyn. 199, 141-155.; J. Cell Biol. 127, 1703-1715.; J. Comp. Neurol. 363, 129-146.; J. Comp. Neurol. 391, 407-428.; J. Neuropathol. Exp. Neurol. 66(12), 1082-1092.

more info or order :

Developmental Studies Hybridoma Bank product webpage