NCAM (sialylated form); PSA-NCAM | Developmental Studies Hybridoma Bank 5A5 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

NCAM (sialylated form); PSA-NCAM

catalog :

5A5

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A5

reactivity :

human, mouse, brown rat, bantam

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:700; loading ...; fig 2f	Carceller H, Guirado R, Nacher J. Dark exposure affects plasticity-related molecules and interneurons throughout the visual system during adulthood. J Comp Neurol. 2019;: pubmed publisher
	Dieriks B, Dean J, Aronica E, Waldvogel H, Faull R, Curtis M. Differential Fatty Acid-Binding Protein Expression in Persistent Radial Glia in the Human and Sheep Subventricular Zone. Dev Neurosci. 2018;40:145-161 pubmed publisher
	Murray H, Swanson M, Dieriks B, Turner C, Faull R, Curtis M. Neurochemical Characterization of PSA-NCAM+ Cells in the Human Brain and Phenotypic Quantification in Alzheimer's Disease Entorhinal Cortex. Neuroscience. 2018;372:289-303 pubmed publisher
	Xue Y, Qian H, Hu J, Zhou B, Zhou Y, Hu X, et al. Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells. Nat Neurosci. 2016;19:807-15 pubmed publisher
	Niu W, Zang T, Smith D, Vue T, Zou Y, Bachoo R, et al. SOX2 reprograms resident astrocytes into neural progenitors in the adult brain. Stem Cell Reports. 2015;4:780-94 pubmed publisher
	Mich J, Signer R, Nakada D, Pineda A, Burgess R, Vue T, et al. Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain. elife. 2014;3:e02669 pubmed publisher
	Darbinyan A, Kaminski R, White M, Darbinian N, Khalili K. Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons. Methods Mol Biol. 2013;1078:45-54 pubmed publisher
	Huang Z, Yu Y, Shimoda Y, Watanabe K, Liu Y. Loss of neural recognition molecule NB-3 delays the normal projection and terminal branching of developing corticospinal tract axons in the mouse. J Comp Neurol. 2012;520:1227-45 pubmed publisher
	Messina D, Alder L, Tresco P. Comparison of pure and mixed populations of human fetal-derived neural progenitors transplanted into intact adult rat brain. Exp Neurol. 2003;184:816-29 pubmed
	Joosten E. Developmental expression of N-CAM epitopes in the rat spinal cord during corticospinal tract axon outgrowth and target innervation. Brain Res Dev Brain Res. 1994;78:226-36 pubmed
	Dodd J, Morton S, Karagogeos D, Yamamoto M, Jessell T. Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons. Neuron. 1988;1:105-16 pubmed
	Acheson A, Sunshine J, Rutishauser U. NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions. J Cell Biol. 1991;114:143-53 pubmed

product information

Internal ID :

2617

Name :

5A5

Depositor Name :

Jessell, T.M. / Dodd, J. / Brenner-Morton, S.

Depositor Institution :

HHMI/Columbia University

Date Deposited :

3/15/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

NCAM (sialylated form); PSA-NCAM

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Chicken,Human,Mouse,Rat

Species Tested (legacy) :

rat, mouse, chicken, human

Initial Publication Pubmed ID :

3272160

Depositor Notes (Special Instructions) :

The 5A5 antibody has successfully been used as a panning substrate for human neural stem cells attachment (PMID: 27680492).

Collections :

Cell adhesion,Cell signaling,Cytoskeleton,Human,Neurodevelopment,Stem cell

Search Keywords :

Jessell, Thomas M / Dodd, Jane, Brenner-Morton, Susan, NCAM (sialylated form); PSA-NCAM, Rat, MIgM, Rat/Mouse/Chicken/Human, Ncam1, Cd56/N-CAM/N-CAM-1/NCAM-1/NCAM-C/NCAMC/Ncam, AB_2314873, monoclonal

Antigen Molecular Weight :

Apparent: 300kD

Gene :

Ncam1

Alternate Gene Name(s) :

Cd56/N-CAM/N-CAM-1/NCAM-1/NCAM-C/NCAMC/Ncam

Uniprot ID :

P13596

Antibody Registry ID :

AB_2314873

Synonyms (Alternate Clone Names) :

CD56

Immunogen :

Embryonic spinal cord membranes (rat)

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

polysialic acid

Epitope Map PubMed ID :

2050739

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

21935948 29429526

Immunofluorescence Pubmed IDs :

14769374 7517802 29680832

Western Blot Pubmed IDs :

23975820 3272160

Additional Information :

This is a very fragile IgM antibody. For optimum activity, we recommend these antibody products be kept at -20C or -80C either frozen in aliquots or with the addition of an equal volume of a cryoprotectant such as glycerol.

DSHB Growth Medium :

Iscove's

References (legacy) :

Neuron 1, 105-116.; Dev. Neurosci. 22, 154-166.; J. Comp. Neurol. 439, 368-383.; Cerebral Cortex 12, 617-624.; Science 297(5586), 1525-1531.; J. Biol. Chem. 288(4), 2623-2631.

more info or order :

Developmental Studies Hybridoma Bank product webpage