product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
LIM/homeobox protein LMX-1.2
catalog :
50.5A5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
50.5A5
reactivity :
mouse, chicken
application :
immunohistochemistry, immunocytochemistry
citations: 8
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; chicken; 1:200; fig s2
Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
Halasi G, Søviknes A, Sigurjonsson O, Glover J. Proliferation and recapitulation of developmental patterning associated with regulative regeneration of the spinal cord neural tube. Dev Biol. 2012;365:118-32 pubmed publisher
Mizuguchi R, Kriks S, Cordes R, Gossler A, Ma Q, Goulding M. Ascl1 and Gsh1/2 control inhibitory and excitatory cell fate in spinal sensory interneurons. Nat Neurosci. 2006;9:770-8 pubmed
Ohyama K, Ellis P, Kimura S, Placzek M. Directed differentiation of neural cells to hypothalamic dopaminergic neurons. Development. 2005;132:5185-97 pubmed
Honig M, Camilli S, Surineni K, Knight B, Hardin H. The contributions of BMP4, positive guidance cues, and repulsive molecules to cutaneous nerve formation in the chick hindlimb. Dev Biol. 2005;282:257-73 pubmed
Liu Y, Helms A, Johnson J. Distinct activities of Msx1 and Msx3 in dorsal neural tube development. Development. 2004;131:1017-28 pubmed
Robb S, SANCHEZ ALVARADO A. Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy. Genesis. 2002;32:293-8 pubmed
Riddle R, Ensini M, Nelson C, Tsuchida T, Jessell T, Tabin C. Induction of the LIM homeobox gene Lmx1 by WNT7a establishes dorsoventral pattern in the vertebrate limb. Cell. 1995;83:631-40 pubmed
product information
Internal ID :
2386
Name :
50.5A5
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
Columbia University, Howard Hughes Medical Institute
Date Deposited :
2/12/99
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
LIM/homeobox protein LMX-1.2
Antigen Species :
chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Mouse,Planaria
Species Tested (legacy) :
chick and planaria
Initial Publication Pubmed ID :
7585966
Depositor Notes (Special Instructions) :
Stains the roof plate, floor plate, dorsal limb bud, and a subset of interneurons (nuclear)
Collections :
Neurodevelopment,Nucleus,Transcription factors
Search Keywords :
Jessell, T.M. / Brenner-Morton, S., LIM/homeobox protein LMX-1.2, chicken, MIgG1, kappa light chain, mouse/planaria, LMX1B, LMX, LMX1, AB_2136850, monoclonal, Transcription Factors/Nucleus/Neurodevelopment, Immunohistochemistry/Immunofluorescence
Antigen Molecular Weight :
Predicted : 45 kDa
Gene :
LMX1B
Alternate Gene Name(s) :
LMX, LMX1
Uniprot ID :
P53413
Antibody Registry ID :
AB_2136850
Immunogen :
Recombinant 6× His-tagged LMX1 protein fragment (LIM domains and homeodomain)
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
Yes
Epitope Location or Sequence :
LIM domains and homeodomain
Epitope Map PubMed ID :
7585966
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunohistochemistry Pubmed IDs :
7585966
Immunofluorescence Pubmed IDs :
7585966 11948917 14973289 27034139 16284116 22370002 15936345 16715081
DSHB Growth Medium :
Iscove's
References (legacy) :
Cell 83, 631-640.; Genesis 32, 293-298.; Development 131, 1017-1028.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US