product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
NCAM
catalog :
5.1H11
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5.1H11
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
more info or order :
citations: 21
Published Application/Species/Sample/Dilution | Reference |
---|---|
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Lee G, Kim H, Elkabetz Y, Al Shamy G, Panagiotakos G, Barberi T, et al. Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells. Nat Biotechnol. 2007;25:1468-75 pubmed
| |
Zhang F, Pomerantz J, Sen G, Palermo A, Blau H. Active tissue-specific DNA demethylation conferred by somatic cell nuclei in stable heterokaryons. Proc Natl Acad Sci U S A. 2007;104:4395-400 pubmed
| |
Park Y, Moon J, Kim J. A comparative study of magnetic-activated cell sorting, cytotoxicity and preplating for the purification of human myoblasts. Yonsei Med J. 2006;47:179-83 pubmed
| |
Terranova R, Pereira C, Du Roure C, Merkenschlager M, Fisher A. Acquisition and extinction of gene expression programs are separable events in heterokaryon reprogramming. J Cell Sci. 2006;119:2065-72 pubmed
| |
Cozzone D, Debard C, Dif N, Ricard N, Disse E, Vouillarmet J, et al. Activation of liver X receptors promotes lipid accumulation but does not alter insulin action in human skeletal muscle cells. Diabetologia. 2006;49:990-9 pubmed
| |
Mesires N, Doumit M. Satellite cell proliferation and differentiation during postnatal growth of porcine skeletal muscle. Am J Physiol Cell Physiol. 2002;282:C899-906 pubmed
| |
Walsh F, Ritter M. Surface antigen differentiation during human myogenesis in culture. Nature. 1981;289:60-4 pubmed
| |
Pavlath G, Blau H. Expression of muscle genes in heterokaryons depends on gene dosage. J Cell Biol. 1986;102:124-30 pubmed
| |
Pavlath G, Rich K, Webster S, Blau H. Localization of muscle gene products in nuclear domains. Nature. 1989;337:570-3 pubmed
| |
Patel K, Moore S, Dickson G, Rossell R, Beverley P, Kemshead J, et al. Neural cell adhesion molecule (NCAM) is the antigen recognized by monoclonal antibodies of similar specificity in small-cell lung carcinoma and neuroblastoma. Int J Cancer. 1989;44:573-8 pubmed
| |
Dantzig J, Hibberd M, Trentham D, Goldman Y. Cross-bridge kinetics in the presence of MgADP investigated by photolysis of caged ATP in rabbit psoas muscle fibres. J Physiol. 1991;432:639-80 pubmed
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product information
Internal ID :
1316
Name :
5.1H11
Depositor Name :
Blau, H.M. / Walsh, F.S.
Depositor Institution :
Stanford University
Depositor Website :
http://web.stanford.edu/group/blau/
Date Deposited :
8/25/93
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
NCAM
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Human
Species Tested (legacy) :
human myoblasts and myotubes
Initial Publication Pubmed ID :
6969855
Depositor Notes (Special Instructions) :
mAb 5.1H11 is specific for NCAM in skeletal muscle and reacts only with human.
Collections :
CD antigens,Cardiac development,Cell adhesion,Cytoskeleton,Human,Neurodevelopment,Skeletal muscle,Stem cell
Search Keywords :
Blau, H.M. / Walsh, F.S., NCAM, Human, MIgG1, kappa light chain, Human, NCAM, NCAM1, CD56, AB_531857, Monoclonal , Human/Cell adhesion/Neurodevelopment/Skeletal Muscle/Cluster determinant antigens, Immunofluorescence/Immunohistochemistry/FACS
Antigen Molecular Weight :
94.5 kDa
Gene :
NCAM
Alternate Gene Name(s) :
NCAM1, CD56
Uniprot ID :
P13591
Antibody Registry ID :
AB_531857
Immunogen :
Fused myoblasts
Clonality :
Monoclonal
Myeloma Strain :
P3xG3 Ag8
Epitope Mapped :
Yes
Epitope Map PubMed ID :
2551826
Recommended Applications :
FACS,Immunofluorescence,Immunohistochemistry
Immunohistochemistry Pubmed IDs :
1886072
Immunofluorescence Pubmed IDs :
6969855 3941151 2915707 22403007 19295131 17360535 16638804 11880278
FACS Pubmed IDs :
26975470 27038912 26395744 22039476 21760887 21602936 19141533 18204829 18037878 16482468 16642545
Additional Information :
5.1H11 has been used extensively to purify human myoblasts using the MACS system. See FACS citations. RRID:AB_531857
DSHB Growth Medium :
Iscove's
References (legacy) :
Nature 289, 60-64.; J. Cell Biol. 102, 124-130.; Nature 337, 570-573.
more info or order :
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