product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
NCAM
catalog :
5.1H11
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5.1H11
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
citations: 21
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; human; fig 2
Aswad H, Jalabert A, Rome S. Depleting extracellular vesicles from fetal bovine serum alters proliferation and differentiation of skeletal muscle cells in vitro. BMC Biotechnol. 2016;16:32 pubmed publisher
Di Filippo E, Mancinelli R, Pietrangelo T, La Rovere R, Quattrocelli M, Sampaolesi M, et al. Myomir dysregulation and reactive oxygen species in aged human satellite cells. Biochem Biophys Res Commun. 2016;473:462-70 pubmed publisher
Laurens C, Louche K, Sengenes C, Coué M, Langin D, Moro C, et al. Adipogenic progenitors from obese human skeletal muscle give rise to functional white adipocytes that contribute to insulin resistance. Int J Obes (Lond). 2016;40:497-506 pubmed publisher
McKay B, Ogborn D, Bellamy L, Tarnopolsky M, Parise G. Myostatin is associated with age-related human muscle stem cell dysfunction. FASEB J. 2012;26:2509-21 pubmed publisher
Long M, Rossi F. Targeted cell fusion facilitates stable heterokaryon generation in vitro and in vivo. PLoS ONE. 2011;6:e26381 pubmed publisher
Sparks L, Moro C, Ukropcova B, Bajpeyi S, Civitarese A, Hulver M, et al. Remodeling lipid metabolism and improving insulin responsiveness in human primary myotubes. PLoS ONE. 2011;6:e21068 pubmed publisher
Walter D, Satheesha S, Albrecht P, Bornhauser B, D Alessandro V, Oesch S, et al. CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres. PLoS ONE. 2011;6:e19506 pubmed publisher
Pomerantz J, Mukherjee S, Palermo A, Blau H. Reprogramming to a muscle fate by fusion recapitulates differentiation. J Cell Sci. 2009;122:1045-53 pubmed publisher
Palermo A, Doyonnas R, Bhutani N, Pomerantz J, Alkan O, Blau H. Nuclear reprogramming in heterokaryons is rapid, extensive, and bidirectional. FASEB J. 2009;23:1431-40 pubmed publisher
Cozzone D, Fröjdö S, Disse E, Debard C, Laville M, Pirola L, et al. Isoform-specific defects of insulin stimulation of Akt/protein kinase B (PKB) in skeletal muscle cells from type 2 diabetic patients. Diabetologia. 2008;51:512-21 pubmed publisher
Lee G, Kim H, Elkabetz Y, Al Shamy G, Panagiotakos G, Barberi T, et al. Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells. Nat Biotechnol. 2007;25:1468-75 pubmed
Zhang F, Pomerantz J, Sen G, Palermo A, Blau H. Active tissue-specific DNA demethylation conferred by somatic cell nuclei in stable heterokaryons. Proc Natl Acad Sci U S A. 2007;104:4395-400 pubmed
Park Y, Moon J, Kim J. A comparative study of magnetic-activated cell sorting, cytotoxicity and preplating for the purification of human myoblasts. Yonsei Med J. 2006;47:179-83 pubmed
Terranova R, Pereira C, Du Roure C, Merkenschlager M, Fisher A. Acquisition and extinction of gene expression programs are separable events in heterokaryon reprogramming. J Cell Sci. 2006;119:2065-72 pubmed
Cozzone D, Debard C, Dif N, Ricard N, Disse E, Vouillarmet J, et al. Activation of liver X receptors promotes lipid accumulation but does not alter insulin action in human skeletal muscle cells. Diabetologia. 2006;49:990-9 pubmed
Mesires N, Doumit M. Satellite cell proliferation and differentiation during postnatal growth of porcine skeletal muscle. Am J Physiol Cell Physiol. 2002;282:C899-906 pubmed
Walsh F, Ritter M. Surface antigen differentiation during human myogenesis in culture. Nature. 1981;289:60-4 pubmed
Pavlath G, Blau H. Expression of muscle genes in heterokaryons depends on gene dosage. J Cell Biol. 1986;102:124-30 pubmed
Pavlath G, Rich K, Webster S, Blau H. Localization of muscle gene products in nuclear domains. Nature. 1989;337:570-3 pubmed
Patel K, Moore S, Dickson G, Rossell R, Beverley P, Kemshead J, et al. Neural cell adhesion molecule (NCAM) is the antigen recognized by monoclonal antibodies of similar specificity in small-cell lung carcinoma and neuroblastoma. Int J Cancer. 1989;44:573-8 pubmed
Dantzig J, Hibberd M, Trentham D, Goldman Y. Cross-bridge kinetics in the presence of MgADP investigated by photolysis of caged ATP in rabbit psoas muscle fibres. J Physiol. 1991;432:639-80 pubmed
product information
Internal ID :
1316
Name :
5.1H11
Depositor Name :
Blau, H.M. / Walsh, F.S.
Depositor Institution :
Stanford University
Depositor Website :
http://web.stanford.edu/group/blau/
Date Deposited :
8/25/93
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
NCAM
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Human
Species Tested (legacy) :
human myoblasts and myotubes
Initial Publication Pubmed ID :
6969855
Depositor Notes (Special Instructions) :
mAb 5.1H11 is specific for NCAM in skeletal muscle and reacts only with human.
Collections :
CD antigens,Cardiac development,Cell adhesion,Cytoskeleton,Human,Neurodevelopment,Skeletal muscle,Stem cell
Search Keywords :
Blau, H.M. / Walsh, F.S., NCAM, Human, MIgG1, kappa light chain, Human, NCAM, NCAM1, CD56, AB_531857, Monoclonal , Human/Cell adhesion/Neurodevelopment/Skeletal Muscle/Cluster determinant antigens, Immunofluorescence/Immunohistochemistry/FACS
Antigen Molecular Weight :
94.5 kDa
Gene :
NCAM
Alternate Gene Name(s) :
NCAM1, CD56
Uniprot ID :
P13591
Antibody Registry ID :
AB_531857
Immunogen :
Fused myoblasts
Clonality :
Monoclonal
Myeloma Strain :
P3xG3 Ag8
Epitope Mapped :
Yes
Epitope Map PubMed ID :
2551826
Recommended Applications :
FACS,Immunofluorescence,Immunohistochemistry
Immunohistochemistry Pubmed IDs :
1886072
Immunofluorescence Pubmed IDs :
6969855 3941151 2915707 22403007 19295131 17360535 16638804 11880278
FACS Pubmed IDs :
26975470 27038912 26395744 22039476 21760887 21602936 19141533 18204829 18037878 16482468 16642545
Additional Information :
5.1H11 has been used extensively to purify human myoblasts using the MACS system. See FACS citations. RRID:AB_531857
DSHB Growth Medium :
Iscove's
References (legacy) :
Nature 289, 60-64.; J. Cell Biol. 102, 124-130.; Nature 337, 570-573.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US