NCAM (cytoplasmic domain), 180 kDa isoform | Developmental Studies Hybridoma Bank 4d product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

NCAM (cytoplasmic domain), 180 kDa isoform

catalog :

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 16

Reference
Cervino A, Paz D, Frontera J. Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis. Dev Neurobiol. 2017;77:1308-1320 pubmed publisher
Corfield J, Eisthen H, Iwaniuk A, Parsons S. Anatomical specializations for enhanced olfactory sensitivity in kiwi, Apteryx mantelli. Brain Behav Evol. 2014;84:214-26 pubmed publisher
Taniguchi Y, Watanabe K, Mochii M. Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole. BMC Dev Biol. 2014;14:27 pubmed publisher
Nagy N, Burns A, Goldstein A. Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum. Dev Dyn. 2012;241:842-51 pubmed publisher
Vivien C, Scerbo P, Girardot F, Le Blay K, Demeneix B, Coen L. Non-viral expression of mouse Oct4, Sox2, and Klf4 transcription factors efficiently reprograms tadpole muscle fibers in vivo. J Biol Chem. 2012;287:7427-35 pubmed publisher
Wuebbles R, Long S, Hanel M, Jones P. Testing the effects of FSHD candidate gene expression in vertebrate muscle development. Int J Clin Exp Pathol. 2010;3:386-400 pubmed
Paz D, Alonso D, Pisano A, Casco V, Knudsen K, Peralta Soler A. Expression of isoforms of the neural cell adhesion molecule (NCAM) and polysialic acid during the development of the Bufo arenarum olfactory system. Int J Dev Biol. 1995;39:1005-13 pubmed
Rafuse V, Landmesser L. Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C. J Cell Biol. 1996;132:969-83 pubmed
Storms S, Anvekar V, Adams L, Murray B. Heterophilic NCAM-mediated cell adhesion to proteoglycans from chick embryonic brain membranes. Exp Cell Res. 1996;223:385-94 pubmed
Saint Jeannet J, Dawid I. Vertical versus planar neural induction in Rana pipiens embryos. Proc Natl Acad Sci U S A. 1994;91:3049-53 pubmed
Byeon M, Sugi Y, Markwald R, Hoffman S. NCAM polypeptides in heart development: association with Z discs of forms that contain the muscle-specific domain. J Cell Biol. 1995;128:209-21 pubmed
Jacobson M, Rutishauser U. Induction of neural cell adhesion molecule (NCAM) in Xenopus embryos. Dev Biol. 1986;116:524-31 pubmed
Sunshine J, Balak K, Rutishauser U, Jacobson M. Changes in neural cell adhesion molecule (NCAM) structure during vertebrate neural development. Proc Natl Acad Sci U S A. 1987;84:5986-90 pubmed
Frelinger A, Rutishauser U. Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes. J Cell Biol. 1986;103:1729-37 pubmed
Watanabe M, Frelinger A, Rutishauser U. Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes. J Cell Biol. 1986;103:1721-7 pubmed
Hall A, Nelson R, Rutishauser U. Binding properties of detergent-solubilized NCAM. J Cell Biol. 1990;110:817-24 pubmed

product information

Internal ID :

1957

Name :

Depositor Name :

Rutishauser, U.

Depositor Institution :

Memorial Sloan-Kettering Cancer Center

Date Deposited :

4/25/90

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

NCAM (cytoplasmic domain), 180 kDa isoform

Antigen Species :

chicken

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Avian,Chicken,Frog,Xenopus

Species Tested (legacy) :

chicken, frog

Initial Publication Pubmed ID :

2430976

Depositor Notes (Special Instructions) :

Stains all neurons with axons

Collections :

Cell adhesion,Cell signaling,Extracellular matrix proteins,Neurodevelopment,Xenopus

Search Keywords :

Rutishauser, U., NCAM (cytoplasmic domain), 180 kDa isoform, chicken, MIgG1, chicken/frog/xenopus/avian, NCAM1, NACM, N-CAM, AB_528389, monoclonal, Cell adhesion/Cell signaling/Xenopus/Extracellular matrix proteins/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA

Antigen Molecular Weight :

Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.

Gene :

NCAM1

Alternate Gene Name(s) :

NCAM, N-CAM

Uniprot ID :

P13590

Antibody Registry ID :

AB_528389

Immunogen :

Purified NCAM from embryonic chicken brain membranes (E14)

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3-X63-Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

The epitope is a polypeptide determinant located on the cytoplasmic region of NCAM ( estimated to be located around 180 aa from C-terminus)

Epitope Map PubMed ID :

2430976 2430977

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

22411589 24941877 25376305 8159704 20490329 28719101

Immunofluorescence Pubmed IDs :

2430976 22232554 8901203

Western Blot Pubmed IDs :

2430976 2430977 8603927 2307708 3475717 7822416 3525283

Immunoprecipitation Pubmed IDs :

8601415 2307708

ELISA Pubmed IDs :

2430976

Additional Information :

Recognizes only the longest isoform (180 kDa)

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 103, 1721-1727.; J. Cell Biol. 103, 1729-1737.; J. Biol. Chem. 287(10), 7427-7435.; Dev. Dyn. 241, 842-851.

more info or order :

Developmental Studies Hybridoma Bank product webpage