product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
fibronectin, type III repeat 9
catalog :
4H2
clonality :
monoclonal
host :
house mouse
conjugate :
nonconjugated
clone name :
4H2
reactivity :
Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 17
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Xenopus laevis; fig 5
Rankin S, Thi Tran H, Wlizla M, Mancini P, Shifley E, Bloor S, et al. A Molecular atlas of Xenopus respiratory system development. Dev Dyn. 2015;244:69-85 pubmed publisher
Zhang Z, Rankin S, Zorn A. Syndecan4 coordinates Wnt/JNK and BMP signaling to regulate foregut progenitor development. Dev Biol. 2016;416:187-199 pubmed publisher
Petridou N, Skourides P. A ligand-independent integrin β1 mechanosensory complex guides spindle orientation. Nat Commun. 2016;7:10899 pubmed publisher
Buisson N, Sirour C, Moreau N, Denker E, Le Bouffant R, Goullancourt A, et al. An adhesome comprising laminin, dystroglycan and myosin IIA is required during notochord development in Xenopus laevis. Development. 2014;141:4569-79 pubmed publisher
Tabler J, Bolger T, Wallingford J, Liu K. Hedgehog activity controls opening of the primary mouth. Dev Biol. 2014;396:1-7 pubmed publisher
Bjerke M, Dzamba B, Wang C, DeSimone D. FAK is required for tension-dependent organization of collective cell movements in Xenopus mesendoderm. Dev Biol. 2014;394:340-56 pubmed publisher
Petridou N, Stylianou P, Skourides P. A dominant-negative provides new insights into FAK regulation and function in early embryonic morphogenesis. Development. 2013;140:4266-76 pubmed publisher
Hara Y, Nagayama K, Yamamoto T, Matsumoto T, Suzuki M, Ueno N. Directional migration of leading-edge mesoderm generates physical forces: Implication in Xenopus notochord formation during gastrulation. Dev Biol. 2013;382:482-95 pubmed publisher
Harata A, Matsuzaki T, Nishikawa A, Ihara S. The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-? signaling. In Vitro Cell Dev Biol Anim. 2013;49:220-9 pubmed publisher
Dzamba B, Jakab K, Marsden M, Schwartz M, DeSimone D. Cadherin adhesion, tissue tension, and noncanonical Wnt signaling regulate fibronectin matrix organization. Dev Cell. 2009;16:421-32 pubmed publisher
Rozario T, Dzamba B, Weber G, Davidson L, DeSimone D. The physical state of fibronectin matrix differentially regulates morphogenetic movements in vivo. Dev Biol. 2009;327:386-98 pubmed publisher
Marsden M, DeSimone D. Integrin-ECM interactions regulate cadherin-dependent cell adhesion and are required for convergent extension in Xenopus. Curr Biol. 2003;13:1182-91 pubmed
Na J, Marsden M, DeSimone D. Differential regulation of cell adhesive functions by integrin alpha subunit cytoplasmic tails in vivo. J Cell Sci. 2003;116:2333-43 pubmed
Marsden M, DeSimone D. Regulation of cell polarity, radial intercalation and epiboly in Xenopus: novel roles for integrin and fibronectin. Development. 2001;128:3635-47 pubmed
Ramos J, Whittaker C, DeSimone D. Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation. Development. 1996;122:2873-83 pubmed
Ramos J, DeSimone D. Xenopus embryonic cell adhesion to fibronectin: position-specific activation of RGD/synergy site-dependent migratory behavior at gastrulation. J Cell Biol. 1996;134:227-40 pubmed
Danker K, Hacke H, Ramos J, DeSimone D, Wedlich D. V(+)-fibronectin expression and localization prior to gastrulation in Xenopus laevis embryos. Mech Dev. 1993;44:155-65 pubmed
product information
Internal ID :
23895
Name :
4H2
Depositor Name :
DeSimone, D.W.
Depositor Institution :
University of Virginia
Depositor Website :
https://med.virginia.edu/cell-biology/lab-snap-shot/desimone-lab/
Date Deposited :
6/23/15
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
fibronectin, type III repeat 9
Antigen Species :
Xenopus laevis
Host Species :
mouse
Isotype :
MIgG1
Positive Tested Species Reactivity :
Xenopus laevis
Initial Publication Pubmed ID :
8698817 8787760
Depositor Notes (Special Instructions) :
4H2 has no known effect on function.
Collections :
Extracellular matrix proteins,Xenopus
Search Keywords :
DeSimone, D.W., Fibronectin, Xenopus laevis, MIgG1, Xenopus laevis, fn1, FN, cig, finc, lets, msf, , Monoclonal, Extracellular matrix proteins/Xenopus, Immunofluorescence/western blot
Antigen Molecular Weight :
Predicted (multiple isoforms):up to 270 kDa; Apparent (reducing conditions): 220 kDa (multiple isoforms)
Gene :
fn1
Alternate Gene Name(s) :
FN, cig, finc, lets, msf
Uniprot ID :
Q91740
Antibody Registry ID :
AB_2721949
Immunogen :
Xenous plasma fibronectin
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
Sp2/O-Ag 14
Epitope Mapped :
Yes
Epitope Location or Sequence :
Type III repeat 9
Epitope Map PubMed ID :
8787760
Recommended Applications :
Immunofluorescence,Western Blot
Immunofluorescence Pubmed IDs :
19289087 12711704 27235146 26952307 25359726 25127991 23435857 25300580 23933171 24048589
Western Blot Pubmed IDs :
8787760 27235146 25127991 8155578 24048589
Pubmed IDs :
11566866 12867028
Additional Information :
RRID:AB_2721949
DSHB Growth Medium :
Iscove's
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US