product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
MSX1+2
catalog :
4G1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4G1
reactivity :
human, mouse, rat, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 18
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:10; loading ...; fig s9
Panaliappan T, Wittmann W, Jidigam V, Mercurio S, Bertolini J, Sghari S, et al. Sox2 is required for olfactory pit formation and olfactory neurogenesis through BMP restriction and Hes5 upregulation. Development. 2018;145: pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 5e
Jang S, Choubey S, Furchtgott L, Zou L, Doyle A, Menon V, et al. Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states. elife. 2017;6: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:20; loading ...; fig 5a
Goodnough L, Dinuoscio G, ATIT R. Twist1 contributes to cranial bone initiation and dermal condensation by maintaining Wnt signaling responsiveness. Dev Dyn. 2016;245:144-56 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig s4
Parchem R, Moore N, Fish J, Parchem J, Braga T, Shenoy A, et al. miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability. Cell Rep. 2015;12:760-73 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig 4
Aoto K, Sandell L, Butler Tjaden N, Yuen K, Watt K, Black B, et al. Mef2c-F10N enhancer driven β-galactosidase (LacZ) and Cre recombinase mice facilitate analyses of gene function and lineage fate in neural crest cells. Dev Biol. 2015;402:3-16 pubmed publisher
Martínez Morales P, Diez del Corral R, Olivera Martínez I, Quiroga A, Das R, Barbas J, et al. FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk. J Cell Biol. 2011;194:489-503 pubmed publisher
Shum A, Tang L, Copp A, Roelink H. Lack of motor neuron differentiation is an intrinsic property of the mouse secondary neural tube. Dev Dyn. 2010;239:3192-203 pubmed publisher
Betters E, Liu Y, Kjaeldgaard A, Sundström E, GARCIA CASTRO M. Analysis of early human neural crest development. Dev Biol. 2010;344:578-92 pubmed publisher
Maier E, Von Hofsten J, Nord H, Fernandes M, Paek H, Hebert J, et al. Opposing Fgf and Bmp activities regulate the specification of olfactory sensory and respiratory epithelial cell fates. Development. 2010;137:1601-11 pubmed publisher
Hu J, Doan L, Currle D, Paff M, Rheem J, Schreyer R, et al. Border formation in a Bmp gradient reduced to single dissociated cells. Proc Natl Acad Sci U S A. 2008;105:3398-403 pubmed publisher
Suzuki M, Satoh A, Ide H, Tamura K. Transgenic Xenopus with prx1 limb enhancer reveals crucial contribution of MEK/ERK and PI3K/AKT pathways in blastema formation during limb regeneration. Dev Biol. 2007;304:675-86 pubmed
Hens J, Dann P, Zhang J, Harris S, Robinson G, Wysolmerski J. BMP4 and PTHrP interact to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Development. 2007;134:1221-30 pubmed
Hassan M, Tare R, Lee S, Mandeville M, Morasso M, Javed A, et al. BMP2 commitment to the osteogenic lineage involves activation of Runx2 by DLX3 and a homeodomain transcriptional network. J Biol Chem. 2006;281:40515-26 pubmed
Lee H, Quinn J, Prasanth K, Swiss V, Economides K, Camacho M, et al. PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein. Genes Dev. 2006;20:784-94 pubmed
Schnapp E, Tanaka E, Tamaka E. Quantitative evaluation of morpholino-mediated protein knockdown of GFP, MSX1, and PAX7 during tail regeneration in Ambystoma mexicanum. Dev Dyn. 2005;232:162-70 pubmed
Hassan M, Javed A, Morasso M, Karlin J, Montecino M, Van Wijnen A, et al. Dlx3 transcriptional regulation of osteoblast differentiation: temporal recruitment of Msx2, Dlx3, and Dlx5 homeodomain proteins to chromatin of the osteocalcin gene. Mol Cell Biol. 2004;24:9248-61 pubmed
Chizhikov V, Millen K. Control of roof plate formation by Lmx1a in the developing spinal cord. Development. 2004;131:2693-705 pubmed
Liem K, Tremml G, Roelink H, Jessell T. Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm. Cell. 1995;82:969-79 pubmed
product information
Internal ID :
2497
Name :
4G1
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
HHMI/Columbia University
Date Deposited :
7/9/97
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
MSX1+2
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Amphibian,Chicken,Human,Mouse,Rat
Species Tested (legacy) :
chicken, rat, mouse, human
Initial Publication Pubmed ID :
7553857
Collections :
Human,Transcription factors
Search Keywords :
Jessell, Thomas M / Brenner-Morton, Susan, MSX1+2, Chicken, MIgG1, Chicken/Rat/Mouse/Human/Amphibian, MSX1,MSX2, HOX-7; CHOX-7; GHOX-7(for MSX1); HOX-8; Msx-2(for MSX2), AB_531788, monoclonal
Antigen Molecular Weight :
Predicted: 30.5 kDa (MSX1-derived protein); 28.2 kDa(MSX2-derived protein)
Gene :
MSX1,MSX2
Alternate Gene Name(s) :
HOX-7; CHOX-7; GHOX-7(for MSX1); HOX-8; Msx-2(for MSX2)
Uniprot ID :
P28362
Antibody Registry ID :
AB_531788
Immunogen Sequence :
chick MSX2 bacterially expressed protein
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
No
Recommended Applications :
Gel Supershift,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
18292231 20392740 15148302 15148302
Immunofluorescence Pubmed IDs :
15580632 20960561 15148302 20478300 21807879 16600910 17303106 17303106 30581079
Western Blot Pubmed IDs :
17301089 15456894 17060321
Chromatin Immunoprecipitation Pubmed IDs :
15456894 17060321
Gel Supershift Pubmed IDs :
15456894 15456894
DSHB Growth Medium :
Iscove's
References (legacy) :
Cell 82, 969-979.; Development 131, 1017-1028.; Dev. Biol. 344(2), 578-592.; Dev. Biol. http://dx.doi.org/10.1016/j.ydbio.2012.04.005
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US