product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Discs large (Drosophila)
catalog :
4F3 anti-discs large
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4F3 anti-discs large
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 50
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; fruit fly; 1:500; fig s2
Chanu S, Sarkar S. Targeted Downregulation of dMyc Suppresses Pathogenesis of Human Neuronal Tauopathies in Drosophila by Limiting Heterochromatin Relaxation and Tau Hyperphosphorylation. Mol Neurobiol. 2017;54:2706-2719 pubmed publisher
  • immunohistochemistry; fruit fly; 1:200; fig 1
Chen K, Lin G, Haelterman N, Ho T, Li T, Li Z, et al. Loss of Frataxin induces iron toxicity, sphingolipid synthesis, and Pdk1/Mef2 activation, leading to neurodegeneration. elife. 2016;5: pubmed publisher
  • immunohistochemistry - paraffin section; fruit fly; 1:100; fig 2b
Woichansky I, Beretta C, Berns N, Riechmann V. Three mechanisms control E-cadherin localization to the zonula adherens. Nat Commun. 2016;7:10834 pubmed publisher
  • immunohistochemistry; fruit fly; 1:1000
Bosveld F, Markova O, Guirao B, Martin C, Wang Z, Pierre A, et al. Epithelial tricellular junctions act as interphase cell shape sensors to orient mitosis. Nature. 2016;530:495-8 pubmed publisher
  • immunohistochemistry; fruit fly; 1:100
Sun D, Buttitta L. Protein phosphatase 2A promotes the transition to G0 during terminal differentiation in Drosophila. Development. 2015;142:3033-45 pubmed publisher
  • immunohistochemistry; fruit fly; 1:200; fig 4
Hagel K, Beriont J, Tessier C. Drosophila Cbp53E Regulates Axon Growth at the Neuromuscular Junction. PLoS ONE. 2015;10:e0132636 pubmed publisher
  • immunohistochemistry; fruit fly; 1:500
Furriols M, Casanova J. Germline and somatic vitelline proteins colocalize in aggregates in the follicular epithelium of Drosophila ovaries. Fly (Austin). 2014;8:113-9 pubmed publisher
  • western blot; fruit fly; 1:5000; fig s8
Merlo P, Frost B, Peng S, Yang Y, Park P, Feany M. p53 prevents neurodegeneration by regulating synaptic genes. Proc Natl Acad Sci U S A. 2014;111:18055-60 pubmed publisher
  • immunohistochemistry; fruit fly; 1:200; fig 2
Castanieto A, Johnston M, Nystul T. EGFR signaling promotes self-renewal through the establishment of cell polarity in Drosophila follicle stem cells. elife. 2014;3: pubmed publisher
Jantrapirom S, Lo Piccolo L, Yoshida H, Yamaguchi M. A new Drosophila model of Ubiquilin knockdown shows the effect of impaired proteostasis on locomotive and learning abilities. Exp Cell Res. 2018;362:461-471 pubmed publisher
Samarasekera G, Auld V. C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src. Mol Biol Cell. 2018;29:123-136 pubmed publisher
Doll C, Vita D, Broadie K. Fragile X Mental Retardation Protein Requirements in Activity-Dependent Critical Period Neural Circuit Refinement. Curr Biol. 2017;27:2318-2330.e3 pubmed publisher
Wang M, Chen P, Wang C, Lai T, Tsai P, Cheng Y, et al. Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response. PLoS Genet. 2016;12:e1006362 pubmed publisher
Chougule A, Hastert M, Thomas J. Drak Is Required for Actomyosin Organization During Drosophila Cellularization. G3 (Bethesda). 2016;6:819-28 pubmed publisher
Lüchtenborg A, Solis G, Egger Adam D, Koval A, Lin C, Blanchard M, et al. Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton. Development. 2014;141:3399-409 pubmed publisher
Chin A, Lin C, Fu T, Dickson B, Chiang A. Diversity and wiring variability of visual local neurons in the Drosophila medulla M6 stratum. J Comp Neurol. 2014;522:3795-816 pubmed publisher
Krzeptowski W, Górska Andrzejak J, Kijak E, Görlich A, Guzik E, Moore G, et al. External and circadian inputs modulate synaptic protein expression in the visual system of Drosophila melanogaster. Front Physiol. 2014;5:102 pubmed publisher
Kumichel A, Knust E. Apical localisation of crumbs in the boundary cells of the Drosophila hindgut is independent of its canonical interaction partner stardust. PLoS ONE. 2014;9:e94038 pubmed publisher
Hopp E, Borst A, Haag J. Subcellular mapping of dendritic activity in optic flow processing neurons. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2014;200:359-70 pubmed publisher
Mhatre S, Satyasi V, Killen M, Paddock B, Moir R, Saunders A, et al. Synaptic abnormalities in a Drosophila model of Alzheimer's disease. Dis Model Mech. 2014;7:373-85 pubmed publisher
Valakh V, Walker L, Skeath J, DiAntonio A. Loss of the spectraplakin short stop activates the DLK injury response pathway in Drosophila. J Neurosci. 2013;33:17863-73 pubmed publisher
Shi W, Chen Y, Gan G, Wang D, Ren J, Wang Q, et al. Brain tumor regulates neuromuscular synapse growth and endocytosis in Drosophila by suppressing mad expression. J Neurosci. 2013;33:12352-63 pubmed publisher
Kern J, Zhang Y, Kramer S, Brenman J, Rasse T. The kinesin-3, unc-104 regulates dendrite morphogenesis and synaptic development in Drosophila. Genetics. 2013;195:59-72 pubmed publisher
Zhao L, Wang D, Wang Q, Rodal A, Zhang Y. Drosophila cyfip regulates synaptic development and endocytosis by suppressing filamentous actin assembly. PLoS Genet. 2013;9:e1003450 pubmed publisher
Natarajan R, Trivedi Vyas D, Wairkar Y. Tuberous sclerosis complex regulates Drosophila neuromuscular junction growth via the TORC2/Akt pathway. Hum Mol Genet. 2013;22:2010-23 pubmed publisher
Lee J, Guan Z, Akbergenova Y, Littleton J. Genetic analysis of synaptotagmin C2 domain specificity in regulating spontaneous and evoked neurotransmitter release. J Neurosci. 2013;33:187-200 pubmed publisher
Tsai P, Wang M, Kao H, Cheng Y, Walker J, Chen R, et al. Neurofibromin mediates FAK signaling in confining synapse growth at Drosophila neuromuscular junctions. J Neurosci. 2012;32:16971-81 pubmed publisher
Boulanger A, Farge M, Ramanoudjame C, Wharton K, Dura J. Drosophila motor neuron retraction during metamorphosis is mediated by inputs from TGF-?/BMP signaling and orphan nuclear receptors. PLoS ONE. 2012;7:e40255 pubmed publisher
Kim Y, Bao H, Bonanno L, Zhang B, Serpe M. Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction. Genes Dev. 2012;26:974-87 pubmed publisher
Neumüller R, Wirtz Peitz F, Lee S, Kwon Y, BUCKNER M, Hoskins R, et al. Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes. Genetics. 2012;190:931-40 pubmed publisher
Graf E, Heerssen H, Wright C, Davis G, DiAntonio A. Stathmin is required for stability of the Drosophila neuromuscular junction. J Neurosci. 2011;31:15026-34 pubmed publisher
Chartier F, Hardy E, Laprise P. Crumbs controls epithelial integrity by inhibiting Rac1 and PI3K. J Cell Sci. 2011;124:3393-8 pubmed publisher
Wang S, Yang J, Tsai A, Kuca T, Sanny J, Lee J, et al. Drosophila adducin regulates Dlg phosphorylation and targeting of Dlg to the synapse and epithelial membrane. Dev Biol. 2011;357:392-403 pubmed publisher
Sun M, Xing G, Yuan L, Gan G, Knight D, With S, et al. Neuroligin 2 is required for synapse development and function at the Drosophila neuromuscular junction. J Neurosci. 2011;31:687-99 pubmed publisher
Lee S, Liu H, Lin W, Guo H, Lu B. LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction. J Neurosci. 2010;30:16959-69 pubmed publisher
Chiang A, Lin C, Chuang C, Chang H, Hsieh C, Yeh C, et al. Three-dimensional reconstruction of brain-wide wiring networks in Drosophila at single-cell resolution. Curr Biol. 2011;21:1-11 pubmed publisher
Astigarraga S, Hofmeyer K, Farajian R, Treisman J. Three Drosophila liprins interact to control synapse formation. J Neurosci. 2010;30:15358-68 pubmed publisher
Krahn M, Bückers J, Kastrup L, Wodarz A. Formation of a Bazooka-Stardust complex is essential for plasma membrane polarity in epithelia. J Cell Biol. 2010;190:751-60 pubmed publisher
Tamori Y, Bialucha C, Tian A, Kajita M, Huang Y, Norman M, et al. Involvement of Lgl and Mahjong/VprBP in cell competition. PLoS Biol. 2010;8:e1000422 pubmed publisher
Ball R, Warren Paquin M, Tsurudome K, Liao E, Elazzouzi F, Cavanagh C, et al. Retrograde BMP signaling controls synaptic growth at the NMJ by regulating trio expression in motor neurons. Neuron. 2010;66:536-49 pubmed publisher
Wang D, Zhang L, Zhao G, Wahlström G, Heino T, Chen J, et al. Drosophila twinfilin is required for cell migration and synaptic endocytosis. J Cell Sci. 2010;123:1546-56 pubmed publisher
Schulte J, Sepp K, Jorquera R, Wu C, Song Y, Hong P, et al. DMob4/Phocein regulates synapse formation, axonal transport, and microtubule organization. J Neurosci. 2010;30:5189-203 pubmed publisher
Graf E, Daniels R, Burgess R, Schwarz T, DiAntonio A. Rab3 dynamically controls protein composition at active zones. Neuron. 2009;64:663-77 pubmed publisher
Rotte C, Walz B, Baumann O. Morphological and functional characterization of the thoracic portion of blowfly salivary glands. Arthropod Struct Dev. 2008;37:372-82 pubmed publisher
Wang X, Shaw W, Tsang H, Reid E, O Kane C. Drosophila spichthyin inhibits BMP signaling and regulates synaptic growth and axonal microtubules. Nat Neurosci. 2007;10:177-85 pubmed
Schulte J, Charish K, Que J, Ravn S, MacKinnon C, Auld V. Gliotactin and Discs large form a protein complex at the tricellular junction of polarized epithelial cells in Drosophila. J Cell Sci. 2006;119:4391-401 pubmed
Dunlop J, Morin X, Corominas M, Serras F, Tear G. glaikit is essential for the formation of epithelial polarity and neuronal development. Curr Biol. 2004;14:2039-45 pubmed
Parnas D, Haghighi A, Fetter R, Kim S, Goodman C. Regulation of postsynaptic structure and protein localization by the Rho-type guanine nucleotide exchange factor dPix. Neuron. 2001;32:415-24 pubmed
Esakowitz L, Yates J. Congenital corneal anaesthesia and the MURCS association: a case report. Br J Ophthalmol. 1988;72:236-8 pubmed
Olive D, al Mufti S, al Mulla W, Khan M, Pasca A, Stanway G, et al. Detection and differentiation of picornaviruses in clinical samples following genomic amplification. J Gen Virol. 1990;71 ( Pt 9):2141-7 pubmed
product information
Internal ID :
3568
Name :
4F3 anti-discs large
Depositor Name :
Goodman, C.
Depositor Institution :
University of California, Berkeley
Date Deposited :
3/14/02
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Discs large (Drosophila)
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila, others not tested
Initial Publication Pubmed ID :
11709153
Collections :
Cardiac development,Drosophila antigens
Search Keywords :
Goodman, Corey, Discs large (Drosophila), Drosophila, MIgG1, Drosophila, dlg1, DLG; DLG-A; DLG1; DlgA; DlgS97; Dmel CG1725; , AB_528203, monoclonal
Antigen Molecular Weight :
Predicted: 106.7 kDa
Gene :
dlg1
Alternate Gene Name(s) :
DLG; DLG-A; DLG1; DlgA; DlgS97; Dmel CG1725;
Uniprot ID :
P31007
Antibody Registry ID :
AB_528203
Synonyms (Alternate Clone Names) :
discs large 1, isoform L
Immunogen :
GST-PDZ2-GST fusion protein; recombinant.
Immunogen Sequence :
same as the antigen
Clonality :
Monoclonal
Myeloma Strain :
P3 x 63 Ag8.653
Epitope Mapped :
Yes
Epitope Location or Sequence :
a.a 439–756
Recommended Applications :
Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
11709153 21159966 20005823 22016536 20819933 21791202 24647929 31353316
Immunofluorescence Pubmed IDs :
15556867 24772085 20819933 24198375 22174071 21084592 23393158 23175848 24647929 23593037 24487408 2170576 27736876 26818071 28756946 30383748 29167383 29247619 24782245 26792857 29706545 29748610 27105580 27145127 31002474 31332509 31325455 31318331 31390561 31387877 31409797
Western Blot Pubmed IDs :
23283333 24772085 27143646 21129968 21791202 20392941 22499592 17032735
Immunoprecipitation Pubmed IDs :
21791202 31353316
Pubmed IDs :
22792255 24710316 23770702 20410372 20644714 23884941 21984807 2451534 20510858 21228178 22499592 2170576
Additional Information :
RRID: AB_528203
DSHB Growth Medium :
RPMI
References (legacy) :
Neuron 32(3), 415-424.; PNAS 102(52), 19027-19032.; Nat. Neurosci. 10(2), 177-185.; Arthropod. Struct. Dev. 37, 372-382.; Development 141, 3399-3409.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US