product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Lim 1+2 / LhxV5
catalog :
4F2
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4F2
reactivity :
little skate, human, mouse, brown rat, bantam, leopard danio, purple urchin
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 60
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; bantam; 1:500; loading ...; fig s8a
Schick E, McCaffery S, Keblish E, Thakurdin C, Emerson M. Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina. Sci Rep. 2019;9:9358 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 6e
Tulloch A, Teo S, Carvajal B, Tessier Lavigne M, Jaworski A. Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3Cre mouse. J Comp Neurol. 2019;527:2948-2972 pubmed publisher
  • immunohistochemistry - frozen section; little skate; fig s1d
Jung H, Baek M, D Elia K, Boisvert C, Currie P, Tay B, et al. The Ancient Origins of Neural Substrates for Land Walking. Cell. 2018;172:667-682.e15 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:40; loading ...; fig 2b
Parmhans N, Sajgo S, Niu J, Luo W, Badea T. Characterization of retinal ganglion cell, horizontal cell, and amacrine cell types expressing the neurotrophic receptor tyrosine kinase Ret. J Comp Neurol. 2018;526:742-766 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 3a
Subashini C, Dhanesh S, Chen C, Riya P, Meera V, Divya T, et al. Wnt5a is a crucial regulator of neurogenesis during cerebellum development. Sci Rep. 2017;7:42523 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 8
Fogarty L, Song B, Suppiah Y, Hasan S, Martin H, Hogan S, et al. Bcl-xL dependency coincides with the onset of neurogenesis in the developing mammalian spinal cord. Mol Cell Neurosci. 2016;77:34-46 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
  • immunocytochemistry; mouse; 1:50; loading ...
Iyer N, Huettner J, Butts J, Brown C, Sakiyama Elbert S. Generation of highly enriched V2a interneurons from mouse embryonic stem cells. Exp Neurol. 2016;277:305-316 pubmed publisher
  • immunohistochemistry - frozen section; bantam; 1:2000; loading ...; tbl 1
Pirson M, Debrulle S, Clippe A, Clotman F, Knoops B. Thioredoxin-2 Modulates Neuronal Programmed Cell Death in the Embryonic Chick Spinal Cord in Basal and Target-Deprived Conditions. PLoS ONE. 2015;10:e0142280 pubmed publisher
  • immunohistochemistry; purple urchin; 1:200; fig 3
Atsuta Y, Takahashi Y. FGF8 coordinates tissue elongation and cell epithelialization during early kidney tubulogenesis. Development. 2015;142:2329-37 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Jin K, Jiang H, Xiao D, Zou M, Zhu J, Xiang M. Tfap2a and 2b act downstream of Ptf1a to promote amacrine cell differentiation during retinogenesis. Mol Brain. 2015;8:28 pubmed publisher
  • immunohistochemistry; mouse; 1:1
Yan Y, Wladyka C, Fujii J, Sockanathan S. Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2. Nat Commun. 2015;6:7006 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 1
Adams K, Rousso D, Umbach J, Novitch B. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Nat Commun. 2015;6:6778 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 6
Maury Y, Côme J, Piskorowski R, Salah Mohellibi N, Chevaleyre V, Peschanski M, et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol. 2015;33:89-96 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 4d
Zou M, Luo H, Xiang M. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn. 2015;244:86-97 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 3
Borromeo M, Meredith D, Castro D, Chang J, Tung K, Guillemot F, et al. A transcription factor network specifying inhibitory versus excitatory neurons in the dorsal spinal cord. Development. 2014;141:2803-12 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100
McLaughlin N, Wang F, Saifudeen Z, El Dahr S. In situ histone landscape of nephrogenesis. Epigenetics. 2014;9:222-35 pubmed publisher
  • immunohistochemistry; bantam
Boije H, Ring H, Shirazi Fard S, Grundberg I, Nilsson M, Hallböök F. Alternative splicing of the chromodomain protein Morf4l1 pre-mRNA has implications on cell differentiation in the developing chicken retina. J Mol Neurosci. 2013;51:615-28 pubmed publisher
Low J, Li P, Chew E, Zhou B, Suzuki K, Zhang T, et al. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Cell Stem Cell. 2019;25:373-387.e9 pubmed publisher
Kemp V, Dautzenberg I, Limpens R, van den Wollenberg D, Hoeben R. Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery. Viruses. 2017;9: pubmed publisher
Heide M, Zhang Y, Zhou X, Zhao T, Miquelajáuregui A, Varela Echavarría A, et al. Lhx5 controls mamillary differentiation in the developing hypothalamus of the mouse. Front Neuroanat. 2015;9:113 pubmed publisher
Shirazi Fard S, Thyselius M, All Ericsson C, Hallböök F. The terminal basal mitosis of chicken retinal Lim1 horizontal cells is not sensitive to cisplatin-induced cell cycle arrest. Cell Cycle. 2014;13:3698-706 pubmed publisher
Inder K, Ruelcke J, Petelin L, Moon H, Choi E, Rae J, et al. Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation. J Extracell Vesicles. 2014;3: pubmed publisher
Hum S, Rymer C, Schaefer C, Bushnell D, Sims Lucas S. Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning. PLoS ONE. 2014;9:e88400 pubmed publisher
Di Giovannantonio L, Di Salvio M, Omodei D, Prakash N, Wurst W, Pierani A, et al. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development. 2014;141:377-88 pubmed publisher
Villanueva S, Contreras F, Tapia A, Carreño J, Vergara C, Ewertz E, et al. Basic fibroblast growth factor reduces functional and structural damage in chronic kidney disease. Am J Physiol Renal Physiol. 2014;306:F430-41 pubmed publisher
Islam M, Li Y, Luo H, Xiang M, Cai L. Meis1 regulates Foxn4 expression during retinal progenitor cell differentiation. Biol Open. 2013;2:1125-36 pubmed publisher
Grinstein M, Yelin R, Herzlinger D, Schultheiss T. Generation of the podocyte and tubular components of an amniote kidney: timing of specification and a role for Wnt signaling. Development. 2013;140:4565-73 pubmed publisher
Christou Y, Ohyama K, Placzek M, Monk P, Shaw P. Wild-type but not mutant SOD1 transgenic astrocytes promote the efficient generation of motor neuron progenitors from mouse embryonic stem cells. BMC Neurosci. 2013;14:126 pubmed publisher
Venters S, Mikawa T, Hyer J. Central and peripheral retina arise through distinct developmental paths. PLoS ONE. 2013;8:e61422 pubmed publisher
Shirazi Fard S, Jarrin M, Boije H, Fillon V, All Eriksson C, Hallböök F. Heterogenic final cell cycle by chicken retinal Lim1 horizontal progenitor cells leads to heteroploid cells with a remaining replicated genome. PLoS ONE. 2013;8:e59133 pubmed publisher
Kim K, Nam J, Mukouyama Y, Kawamoto S. Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development. J Cell Biol. 2013;200:443-58 pubmed publisher
Kawaue T, Okamoto M, Matsuyo A, Inoue J, Ueda Y, Tomonari S, et al. Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken. Biol Open. 2012;1:1083-93 pubmed publisher
Galdo M, Gregonis J, Fiore C, Compagnone N. Dehydroepiandrosterone biosynthesis, role, and mechanism of action in the developing neural tube. Front Endocrinol (Lausanne). 2012;3:16 pubmed publisher
Bello S, Millo H, Rajebhosale M, Price S. Catenin-dependent cadherin function drives divisional segregation of spinal motor neurons. J Neurosci. 2012;32:490-505 pubmed publisher
Espana A, Clotman F. Onecut transcription factors are required for the second phase of development of the A13 dopaminergic nucleus in the mouse. J Comp Neurol. 2012;520:1424-41 pubmed publisher
Laurent M, Maryvonne L, Le Dreau G, Gwenvaël L, Guillonneau X, Xavier G, et al. Temporal and spatial expression of CCN3 during retina development. Dev Neurobiol. 2012;72:1363-75 pubmed publisher
Emerson M, Cepko C. Identification of a retina-specific Otx2 enhancer element active in immature developing photoreceptors. Dev Biol. 2011;360:241-55 pubmed publisher
Waite M, Skaggs K, Kaviany P, Skidmore J, Causeret F, Martin J, et al. Distinct populations of GABAergic neurons in mouse rhombomere 1 express but do not require the homeodomain transcription factor PITX2. Mol Cell Neurosci. 2012;49:32-43 pubmed publisher
Lai H, Klisch T, Roberts R, Zoghbi H, Johnson J. In vivo neuronal subtype-specific targets of Atoh1 (Math1) in dorsal spinal cord. J Neurosci. 2011;31:10859-71 pubmed publisher
Gutierrez C, McNally M, Canto Soler M. Cytoskeleton proteins previously considered exclusive to ganglion cells are transiently expressed by all retinal neuronal precursors. BMC Dev Biol. 2011;11:46 pubmed publisher
de Melo J, Peng G, Chen S, Blackshaw S. The Spalt family transcription factor Sall3 regulates the development of cone photoreceptors and retinal horizontal interneurons. Development. 2011;138:2325-36 pubmed publisher
Fotaki V, Price D, Mason J. Wnt/?-catenin signaling is disrupted in the extra-toes (Gli3(Xt/Xt) ) mutant from early stages of forebrain development, concomitant with anterior neural plate patterning defects. J Comp Neurol. 2011;519:1640-57 pubmed publisher
Wahlin K, Hackler L, Adler R, Zack D. Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina. J Comp Neurol. 2010;518:4938-62 pubmed publisher
Miyata T, Ono Y, Okamoto M, Masaoka M, Sakakibara A, Kawaguchi A, et al. Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum. Neural Dev. 2010;5:23 pubmed publisher
Kastanenka K, Landmesser L. In vivo activation of channelrhodopsin-2 reveals that normal patterns of spontaneous activity are required for motoneuron guidance and maintenance of guidance molecules. J Neurosci. 2010;30:10575-85 pubmed publisher
Asli N, Kessel M. Spatiotemporally restricted regulation of generic motor neuron programs by miR-196-mediated repression of Hoxb8. Dev Biol. 2010;344:857-68 pubmed publisher
Yang M, Cagle M, Honig M. Identification of cerebellin2 in chick and its preferential expression by subsets of developing sensory neurons and their targets in the dorsal horn. J Comp Neurol. 2010;518:2818-40 pubmed publisher
Peltopuro P, Kala K, Partanen J. Distinct requirements for Ascl1 in subpopulations of midbrain GABAergic neurons. Dev Biol. 2010;343:63-70 pubmed publisher
Iulianella A, Sharma M, Vanden Heuvel G, Trainor P. Cux2 functions downstream of Notch signaling to regulate dorsal interneuron formation in the spinal cord. Development. 2009;136:2329-34 pubmed publisher
Yu T, Fotaki V, Mason J, Price D. Analysis of early ventral telencephalic defects in mice lacking functional Gli3 protein. J Comp Neurol. 2009;512:613-27 pubmed publisher
Edqvist P, Lek M, Boije H, Lindbäck S, Hallböök F. Axon-bearing and axon-less horizontal cell subtypes are generated consecutively during chick retinal development from progenitors that are sensitive to follistatin. BMC Dev Biol. 2008;8:46 pubmed publisher
Iulianella A, Sharma M, Durnin M, Vanden Heuvel G, Trainor P. Cux2 (Cutl2) integrates neural progenitor development with cell-cycle progression during spinal cord neurogenesis. Development. 2008;135:729-41 pubmed publisher
Hori K, Cholewa Waclaw J, Nakada Y, Glasgow S, Masui T, Henke R, et al. A nonclassical bHLH Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling. Genes Dev. 2008;22:166-78 pubmed publisher
Theveneau E, Duband J, Altabef M. Ets-1 confers cranial features on neural crest delamination. PLoS ONE. 2007;2:e1142 pubmed
Ungos J, Karlstrom R, Raible D. Hedgehog signaling is directly required for the development of zebrafish dorsal root ganglia neurons. Development. 2003;130:5351-62 pubmed
Fedtsova N, Turner E. Signals from the ventral midline and isthmus regulate the development of Brn3.0-expressing neurons in the midbrain. Mech Dev. 2001;105:129-44 pubmed
Lamar E, Kintner C, Goulding M. Identification of NKL, a novel Gli-Kruppel zinc-finger protein that promotes neuronal differentiation. Development. 2001;128:1335-46 pubmed
Tsuchida T, Ensini M, Morton S, Baldassare M, Edlund T, Jessell T, et al. Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes. Cell. 1994;79:957-70 pubmed
De Marco C, Cini C, Coccia R, Blarzino C. Synthesis and chromatographic properties of selenazolidine-2-carboxylic acid (beta-selenaproline). Ital J Biochem. 1979;28:104-10 pubmed
Miner J, Conti R. Topical nitroglycerin for ischemic heart disease. JAMA. 1978;239:2166-7 pubmed
product information
Internal ID :
2491
Name :
4F2
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
HHMI/Columbia University
Date Deposited :
7/9/97
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Lim 1+2 / LhxV5
Antigen Species :
Rat
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Chicken,Fish,Human,Mouse,Rat,Zebrafish
Species Tested (legacy) :
chick, rat, mouse
Initial Publication Pubmed ID :
7528105
Collections :
Cell adhesion,Neurodevelopment,Transcription factors
Search Keywords :
Jessell, Thomas M / Brenner-Morton, Susan, Lim 1+2 / LhxV5, Rat, MIgG1, Chicken/Rat/Mouse/Human/Zebrafish, Lhx1, LIM homeobox 1, AB_531784, monoclonal
Antigen Molecular Weight :
Predicted: 44.8 kDa
Gene :
Lhx1
Alternate Gene Name(s) :
LIM homeobox 1
Uniprot ID :
P63007
Antibody Registry ID :
AB_531784
Synonyms (Alternate Clone Names) :
LIM/homeobox protein Lhx1, rlim, LIM homeobox protein 1, homeobox protein Lim-1
Immunogen :
Lim2/LhxV5 (rat); recombinant.
Immunogen Sequence :
1-360 aa
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
No
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
7528105 21031560 11262234 0553899 24285501 24134124 23213388 24154527
Immunofluorescence Pubmed IDs :
21963459 11262234 20686000 21795538 0417196 20506477 24154527 11429288 31303547
Western Blot Pubmed IDs :
25018864 22102297
FFPE Pubmed IDs :
24154527 21925604 11429288
Pubmed IDs :
13129844 24244849 22649409 22238085 20417196 20553899 22038708 18223201 19048639 23613848 24335253 20417196 19542352 25018864 21452227 21925604 18198335
DSHB Growth Medium :
Iscove's
References (legacy) :
Cell 79, 957-970.; Genes Dev. 16, 548-553.; Development 131, 1319-1330.; Development 131, 1017-1028.; Nat. Neurosci. 8(11), 1510-1515.; Dev. Biol. 288, 571-581.; Genes Dev. 21, 531-536.; Genes Dev. 22, 3320-3334.; Dev. Biol. 343, 63-70.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US