Engrailed/invected gene products | Developmental Studies Hybridoma Bank 4D9 anti-engrailed/invected product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Engrailed/invected gene products

catalog :

4D9 anti-engrailed/invected

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4D9 anti-engrailed/invected

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 51

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:5	Lacin H, Truman J. Lineage mapping identifies molecular and architectural similarities between the larval and adult Drosophila central nervous system. elife. 2016;5:e13399 pubmed publisher
immunohistochemistry; fruit fly; 1:50; fig 5	Gurdziel K, Lorberbaum D, Udager A, Song J, Richards N, Parker D, et al. Identification and Validation of Novel Hedgehog-Responsive Enhancers Predicted by Computational Analysis of Ci/Gli Binding Site Density. PLoS ONE. 2015;10:e0145225 pubmed publisher
immunohistochemistry; fruit fly; 1:50	Moris Sanz M, Estacio Gómez A, Alvarez Rivero J, Diaz Benjumea F. Specification of neuronal subtypes by different levels of Hunchback. Development. 2014;141:4366-74 pubmed publisher
	Zhu J, Ordway A, Weber L, Buddika K, Kumar J. Polycomb group (PcG) proteins and Pax6 cooperate to inhibit in vivo reprogramming of the developing Drosophila eye. Development. 2018;145: pubmed publisher
	Venero Galanternik M, Lush M, Piotrowski T. Glypican4 modulates lateral line collective cell migration non cell-autonomously. Dev Biol. 2016;419:321-335 pubmed publisher
	Prasad N, Tarikere S, Khanale D, Habib F, Shashidhara L. A comparative genomic analysis of targets of Hox protein Ultrabithorax amongst distant insect species. Sci Rep. 2016;6:27885 pubmed publisher
	Barton L, Lovander K, Pinto B, Geyer P. Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin. Dev Biol. 2016;415:75-86 pubmed publisher
	Guillon E, Bretaud S, Ruggiero F. Slow Muscle Precursors Lay Down a Collagen XV Matrix Fingerprint to Guide Motor Axon Navigation. J Neurosci. 2016;36:2663-76 pubmed publisher
	Pezier A, Jezzini S, Marie B, Blagburn J. Engrailed alters the specificity of synaptic connections of Drosophila auditory neurons with the giant fiber. J Neurosci. 2014;34:11691-704 pubmed publisher
	Heckscher E, Long F, Layden M, Chuang C, Manning L, Richart J, et al. Atlas-builder software and the eNeuro atlas: resources for developmental biology and neuroscience. Development. 2014;141:2524-32 pubmed publisher
	Herrera S, Morata G. Transgressions of compartment boundaries and cell reprogramming during regeneration in Drosophila. elife. 2014;3:e01831 pubmed publisher
	Eliazer S, Palacios V, Wang Z, Kollipara R, Kittler R, Buszczak M. Lsd1 restricts the number of germline stem cells by regulating multiple targets in escort cells. PLoS Genet. 2014;10:e1004200 pubmed publisher
	Lacin H, Rusch J, Yeh R, Fujioka M, Wilson B, Zhu Y, et al. Genome-wide identification of Drosophila Hb9 targets reveals a pivotal role in directing the transcriptome within eight neuronal lineages, including activation of nitric oxide synthase and Fd59a/Fox-D. Dev Biol. 2014;388:117-33 pubmed publisher
	Heffer A, Grubbs N, Mahaffey J, Pick L. The evolving role of the orphan nuclear receptor ftz-f1, a pair-rule segmentation gene. Evol Dev. 2013;15:406-17 pubmed publisher
	Fero K, Bergeron S, Horstick E, Codore H, Li G, Ono F, et al. Impaired embryonic motility in dusp27 mutants reveals a developmental defect in myofibril structure. Dis Model Mech. 2014;7:289-98 pubmed publisher
	Pezier A, Blagburn J. Auditory responses of engrailed and invected-expressing Johnston's Organ neurons in Drosophila melanogaster. PLoS ONE. 2013;8:e71419 pubmed publisher
	Da Ros V, Gutiérrez Pérez I, Ferres Marco D, Dominguez M. Dampening the signals transduced through hedgehog via microRNA miR-7 facilitates notch-induced tumourigenesis. PLoS Biol. 2013;11:e1001554 pubmed publisher
	Azzam G, Liu J. Only one isoform of Drosophila melanogaster CTP synthase forms the cytoophidium. PLoS Genet. 2013;9:e1003256 pubmed publisher
	Cantu J, Flowers G, Topczewski J. Notum homolog plays a novel role in primary motor innervation. J Neurosci. 2013;33:2177-87 pubmed publisher
	Seong K, Ishii S. Su(fu) switches Rdx functions to fine-tune hedgehog signaling in the Drosophila wing disk. Genes Cells. 2013;18:66-78 pubmed publisher
	Aguilar Hidalgo D, Domínguez Cejudo M, Amore G, Brockmann A, Lemos M, Cordoba A, et al. A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes. Development. 2013;140:82-92 pubmed publisher
	Fontana J, Crews S. Transcriptome analysis of Drosophila CNS midline cells reveals diverse peptidergic properties and a role for castor in neuronal differentiation. Dev Biol. 2012;372:131-42 pubmed publisher
	Ayers K, Mteirek R, Cervantes A, Lavenant Staccini L, Therond P, Gallet A. Dally and Notum regulate the switch between low and high level Hedgehog pathway signalling. Development. 2012;139:3168-79 pubmed publisher
	Fujioka M, Gebelein B, COFER Z, Mann R, Jaynes J. Engrailed cooperates directly with Extradenticle and Homothorax on a distinct class of homeodomain binding sites to repress sloppy paired. Dev Biol. 2012;366:382-92 pubmed publisher
	Gutierrez L, Oktaba K, Scheuermann J, Gambetta M, Ly Hartig N, Muller J. The role of the histone H2A ubiquitinase Sce in Polycomb repression. Development. 2012;139:117-27 pubmed publisher
	Perez L, Barrio L, Cano D, Fiuza U, Muzzopappa M, Milan M. Enhancer-PRE communication contributes to the expansion of gene expression domains in proliferating primordia. Development. 2011;138:3125-34 pubmed publisher
	Demilly A, Simionato E, Ohayon D, Kerner P, GarcÃ¨s A, Vervoort M. Coe genes are expressed in differentiating neurons in the central nervous system of protostomes. PLoS ONE. 2011;6:e21213 pubmed publisher
	Momota R, Naito I, Ninomiya Y, Ohtsuka A. Drosophila type XV/XVIII collagen, Mp, is involved in Wingless distribution. Matrix Biol. 2011;30:258-66 pubmed publisher
	Kohwi M, Hiebert L, Doe C. The pipsqueak-domain proteins Distal antenna and Distal antenna-related restrict Hunchback neuroblast expression and early-born neuronal identity. Development. 2011;138:1727-35 pubmed publisher
	Benito Sipos J, Estacio Gómez A, Moris Sanz M, Baumgardt M, Thor S, Diaz Benjumea F. A genetic cascade involving klumpfuss, nab and castor specifies the abdominal leucokinergic neurons in the Drosophila CNS. Development. 2010;137:3327-36 pubmed publisher
	Thomsen S, Azzam G, Kaschula R, Williams L, Alonso C. Developmental RNA processing of 3'UTRs in Hox mRNAs as a context-dependent mechanism modulating visibility to microRNAs. Development. 2010;137:2951-60 pubmed publisher
	Ruiz S, Rickert C, Berger C, Technau G, Cantera R. Spatio-temporal pattern of cells expressing the clock genes period and timeless and the lineages of period expressing neurons in the embryonic CNS of Drosophila melanogaster. Gene Expr Patterns. 2010;10:274-82 pubmed publisher
	Braid L, Lee W, Uetrecht A, Swarup S, Papaianni G, Heiler A, et al. Nemo phosphorylates Even-skipped and promotes Eve-mediated repression of odd-skipped in even parasegments during Drosophila embryogenesis. Dev Biol. 2010;343:178-89 pubmed publisher
	Wizenmann A, Brunet I, Lam J, Sonnier L, Beurdeley M, Zarbalis K, et al. Extracellular Engrailed participates in the topographic guidance of retinal axons in vivo. Neuron. 2009;64:355-366 pubmed publisher
	Ayers K, Rodriguez R, Gallet A, Ruel L, Therond P. Tow (Target of Wingless), a novel repressor of the Hedgehog pathway in Drosophila. Dev Biol. 2009;329:280-93 pubmed publisher
	Wheeler S, Banerjee S, Blauth K, Rogers S, Bhat M, Crews S. Neurexin IV and Wrapper interactions mediate Drosophila midline glial migration and axonal ensheathment. Development. 2009;136:1147-57 pubmed publisher
	Baratte S, Andouche A, Bonnaud L. Engrailed in cephalopods: a key gene related to the emergence of morphological novelties. Dev Genes Evol. 2007;217:353-62 pubmed
	Waldrop S, Chan C, Cagatay T, Zhang S, Rousset R, Mack J, et al. An unconventional nuclear localization motif is crucial for function of the Drosophila Wnt/wingless antagonist Naked cuticle. Genetics. 2006;174:331-48 pubmed
	Kent D, Bush E, Hooper J. Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptus. Development. 2006;133:2001-10 pubmed
	Stehr C, Linbo T, Incardona J, Scholz N. The developmental neurotoxicity of fipronil: notochord degeneration and locomotor defects in zebrafish embryos and larvae. Toxicol Sci. 2006;92:270-8 pubmed
	Harzsch S. Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida). Dev Biol. 2003;258:44-56 pubmed
	Nakamura Y, Haines N, Chen J, Okajima T, Furukawa K, Urano T, et al. Identification of a Drosophila gene encoding xylosylprotein beta4-galactosyltransferase that is essential for the synthesis of glycosaminoglycans and for morphogenesis. J Biol Chem. 2002;277:46280-8 pubmed
	Belenkaya T, Han C, Standley H, Lin X, Houston D, Heasman J, et al. pygopus Encodes a nuclear protein essential for wingless/Wnt signaling. Development. 2002;129:4089-101 pubmed
	Nagaso H, Murata T, Day N, Yokoyama K. Simultaneous detection of RNA and protein by in situ hybridization and immunological staining. J Histochem Cytochem. 2001;49:1177-82 pubmed
	Stauber M, Taubert H, Schmidt Ott U. Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae). Proc Natl Acad Sci U S A. 2000;97:10844-9 pubmed
	Yu X, Waltzer L, Bienz M. A new Drosophila APC homologue associated with adhesive zones of epithelial cells. Nat Cell Biol. 1999;1:144-51 pubmed
	Muller H, Samanta R, Wieschaus E. Wingless signaling in the Drosophila embryo: zygotic requirements and the role of the frizzled genes. Development. 1999;126:577-86 pubmed
	White P, Aberle H, Vincent J. Signaling and adhesion activities of mammalian beta-catenin and plakoglobin in Drosophila. J Cell Biol. 1998;140:183-95 pubmed
	Serrano N, Brock H, Demeret C, Dura J, Randsholt N, Kornberg T, et al. polyhomeotic appears to be a target of engrailed regulation in Drosophila. Development. 1995;121:1691-703 pubmed
	Baird A, Cuthbert A, MacVinish L. Type 1 hypersensitivity reactions in reconstructed tissues using syngeneic cell types. Br J Pharmacol. 1987;91:857-69 pubmed
	Patel N, Martin Blanco E, Coleman K, Poole S, Ellis M, Kornberg T, et al. Expression of engrailed proteins in arthropods, annelids, and chordates. Cell. 1989;58:955-68 pubmed

product information

Internal ID :

3562

Name :

4D9 anti-engrailed/invected

Depositor Name :

Goodman, C.

Depositor Institution :

University of California, Berkeley

Date Deposited :

2/1/94

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Engrailed/invected gene products

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Beetle,Chicken,Crayfish,Drosophila,Fish,Grasshopper,Lobster,Molluscs,Worm,Xenopus,Zebrafish

Species Tested (legacy) :

Drosophila, grasshopper, crayfish, lobster, zebrafish, chicken, Xenopus; not mouse and moth

Initial Publication Pubmed ID :

2570637

Depositor Notes (Special Instructions) :

This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons. The antibody doesn't recognize engrailed in mouse, human, sea urchin and Manduca sexta (moth).

Collections :

Drosophila antigens,Transcription factors,Xenopus

Search Keywords :

Goodman, Corey, Engrailed/invected gene products, Drosophila, MIgG1, Drosophila/Grasshopper/CrayFish/Lobster/Zebrafish/Chicken/Xenopus/Worm/Molluscs , En1, En-1, Mo-en.1, AB_528224, monoclonal

Antigen Molecular Weight :

Predicted: 59 kDa for en; 61 kDa for in

Gene :

en & in

Uniprot ID :

P02836 P05527

Antibody Registry ID :

AB_528224

Synonyms (Alternate Clone Names) :

4D9

Immunogen :

Invected (C-terminal two-thirds of the invected protein); recombinant.

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 38-48 of the engrailed homeobox (C-terminal aa 490-500 of engrailed: ELGLNEAQIKI; aa 507-517 of invected)

Epitope Map PubMed ID :

2570637

Recommended Applications :

Chromatin Immunoprecipitation,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunohistochemistry Pubmed IDs :

2570637 2570637 9425166 10559900 10995461 11511686 12215432 12781681 17394016 20398650 21477650 27296678 30995487

Immunofluorescence Pubmed IDs :

9876186 10995461 11511686 12781681 20398650 22537495 23154412 19270173 19914184 7600986 27296678 26937007 27640326 27174470 24917506 23459760 29530880 27095493

Western Blot Pubmed IDs :

2570637

Immunoprecipitation Pubmed IDs :

19270173

Function Blocking Pubmed IDs :

2570637 19914184

Gel Supershift Pubmed IDs :

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 58, 955-968.; Nature 367, 429-434.; J. Cell Biol. 140(1), 183-195.; Nature Cell Biol. 1(3), 144-151.; Development 126, 577-586.; Proc. Natl. Acad. Sci. 97(20), 10844-10849.; J. Histochem. Cytochem. 49(9), 1177-1182.; J. Biol. Chem. 277(48), 46280-46288.; Dev. Biol. 258, 44-56.; Dev. Genes Evol. 217, 353-362.; Matrix Biol. 30, 258-266.; Dev. Biol. 366, 382-392.; Development 140(1), 82-92.

more info or order :

Developmental Studies Hybridoma Bank product webpage