Collagen, Type IX | Developmental Studies Hybridoma Bank 4D6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Collagen, Type IX

catalog :

4D6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4D6

reactivity :

mouse, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

product information

Internal ID :

3424

Name :

4D6

Depositor Name :

Mayne, R.

Depositor Institution :

University of Alabama at Birmingham

Date Deposited :

5/20/93

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Collagen, Type IX

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chick,Mouse

Species Tested (legacy) :

chicken

Initial Publication Pubmed ID :

2411737

Depositor Notes (Special Instructions) :

Hyaluronidase digestion (1 mg/ml at 37? in PBS pH 5.9 for 30 minutes) may be required for expose the 4D6 epitope.

Collections :

Extracellular matrix proteins

Search Keywords :

Mayne, R. , Collagen, Type IX, Chicken, MIgG1, Chick/Mouse, COL9A3, , AB_528172, Monoclonal, Extracellular Matrix, Immunohistochemistry/Western blot/Immunofluorescence/ELISA

Antigen Molecular Weight :

63.0 kDa

Gene :

COL9A3

Uniprot ID :

P32017

Antibody Registry ID :

AB_528172

Immunogen :

Purified pepsin-digested sterna

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63A8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

Junction of NC4 and N-terminus of COL3 domains

Epitope Map PubMed ID :

2411737 2186687

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

3294062 1379142 1281696 2015794 2643540 2680423 2468720

Immunofluorescence Pubmed IDs :

2411737 3294062 1379142 10094835 3050996 9603421 8112996 8462746 7881125 11273670

Western Blot Pubmed IDs :

2411737 3346333 2002024

ELISA Pubmed IDs :

2411737

Additional Information :

RRID:AB_2267538

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 101, 814-823.; Dev. Biol. 128, 396-405.; J. Cell Biol. 106, 991-997.; J. Biol. Chem. 266, 4752-4757.; Development 115, 169-179.; Exp. Cell Res. 248, 294-305.

more info or order :

Developmental Studies Hybridoma Bank product webpage