product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Wingless protein
catalog :
4D4
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4D4
reactivity :
mouse, Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 66
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Drosophila melanogaster; 1:1; loading ...; fig 4a
Kopke D, Leahy S, Vita D, Lima S, Newman Z, Broadie K. Carrier of Wingless (Cow) Regulation of Drosophila Neuromuscular Junction Development. Eneuro. 2020;7: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:200; loading ...; fig 3-s3c
Lebensohn A, Dubey R, Neitzel L, Tacchelly Benites O, Yang E, Marceau C, et al. Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling. elife. 2016;5: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:200; fig 2
Liu M, Li Y, Liu A, Li R, Su Y, Du J, et al. The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development. elife. 2016;5: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:500; fig 1a
Huang D, Li X, Sun L, Huang P, Ying H, Wang H, et al. Regulation of Hippo signalling by p38 signalling. J Mol Cell Biol. 2016;8:328-37 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:50; fig 5
Carrasco Rando M, Atienza Manuel A, Martin P, Burke R, Ruiz Gomez M. Fear-of-intimacy-mediated zinc transport controls the function of zinc-finger transcription factors involved in myogenesis. Development. 2016;143:1948-57 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:20; fig s8
Linnemannstöns K, Ripp C, Honemann Capito M, Brechtel Curth K, Hedderich M, Wodarz A. The PTK7-related transmembrane proteins off-track and off-track 2 are co-receptors for Drosophila Wnt2 required for male fertility. PLoS Genet. 2014;10:e1004443 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:1000
Galagovsky D, Katz M, Acevedo J, Sorianello E, Glavic A, Wappner P. The Drosophila insulin-degrading enzyme restricts growth by modulating the PI3K pathway in a cell-autonomous manner. Mol Biol Cell. 2014;25:916-24 pubmed publisher
Won J, Kim G, Kim J, Cho D, Kwon B, Bae Y, et al. ADAMTS Sol narae cleaves extracellular Wingless to generate a novel active form that regulates cell proliferation in Drosophila. Cell Death Dis. 2019;10:564 pubmed publisher
Wolf D, Smylla T, Reichmuth J, Hoffmeister P, Kober L, Zimmermann M, et al. Nucleo-cytoplasmic shuttling of Drosophila Hairless/Su(H) heterodimer as a means of regulating Notch dependent transcription. Biochim Biophys Acta Mol Cell Res. 2019;1866:1520-1532 pubmed publisher
Pinal N, Martin M, Medina I, Morata G. Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis. Nat Commun. 2018;9:1541 pubmed publisher
Zhu J, Ordway A, Weber L, Buddika K, Kumar J. Polycomb group (PcG) proteins and Pax6 cooperate to inhibit in vivo reprogramming of the developing Drosophila eye. Development. 2018;145: pubmed publisher
Muzzopappa M, Murcia L, Milan M. Feedback amplification loop drives malignant growth in epithelial tissues. Proc Natl Acad Sci U S A. 2017;114:E7291-E7300 pubmed publisher
Alibardi L. Wnt-1 immunodetection in the regenerating tail of lizard suggests it is involved in the proliferation and distal growth of the blastema. Acta Histochem. 2017;119:211-219 pubmed publisher
Verghese S, Su T. Drosophila Wnt and STAT Define Apoptosis-Resistant Epithelial Cells for Tissue Regeneration after Irradiation. PLoS Biol. 2016;14:e1002536 pubmed publisher
Michel M, Aliee M, Rudolf K, Bialas L, Jülicher F, Dahmann C. The Selector Gene apterous and Notch Are Required to Locally Increase Mechanical Cell Bond Tension at the Drosophila Dorsoventral Compartment Boundary. PLoS ONE. 2016;11:e0161668 pubmed publisher
Tang W, Wang D, Shen J. Asymmetric distribution of Spalt in Drosophila wing squamous and columnar epithelia ensures correct cell morphogenesis. Sci Rep. 2016;6:30236 pubmed publisher
Fu C, Wang X, Cheng Q, Wang D, Hirose S, Liu Q. The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila. Sci Rep. 2016;6:27981 pubmed publisher
Urbach R, Jussen D, Technau G. Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila. Development. 2016;143:1290-301 pubmed publisher
Waldron J, Jones C, Towler B, Pashler A, Grima D, Hebbes S, et al. Xrn1/Pacman affects apoptosis and regulates expression of hid and reaper. Biol Open. 2015;4:649-60 pubmed publisher
Palmer W, Jia D, Deng W. Cis-interactions between Notch and its ligands block ligand-independent Notch activity. elife. 2014;3: pubmed publisher
Bassett A, Azzam G, Wheatley L, Tibbit C, Rajakumar T, McGowan S, et al. Understanding functional miRNA-target interactions in vivo by site-specific genome engineering. Nat Commun. 2014;5:4640 pubmed publisher
Herranz H, Weng R, Cohen S. Crosstalk between epithelial and mesenchymal tissues in tumorigenesis and imaginal disc development. Curr Biol. 2014;24:1476-84 pubmed publisher
Pradhan Sundd T, Verheyen E. The role of Bro1- domain-containing protein Myopic in endosomal trafficking of Wnt/Wingless. Dev Biol. 2014;392:93-107 pubmed publisher
Harumoto T, Anbutsu H, Fukatsu T. Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway. PLoS Pathog. 2014;10:e1003956 pubmed publisher
Kanca O, Caussinus E, Denes A, Percival Smith A, Affolter M. Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development. 2014;141:472-80 pubmed publisher
Spurlin J, Lwigale P. Wounded embryonic corneas exhibit nonfibrotic regeneration and complete innervation. Invest Ophthalmol Vis Sci. 2013;54:6334-44 pubmed publisher
Petrik D, Yun S, Latchney S, Kamrudin S, Leblanc J, Bibb J, et al. Early postnatal in vivo gliogenesis from nestin-lineage progenitors requires cdk5. PLoS ONE. 2013;8:e72819 pubmed publisher
Mitchell N, Lin J, Zaytseva O, Cranna N, Lee A, Quinn L. The Ecdysone receptor constrains wingless expression to pattern cell cycle across the Drosophila wing margin in a Cyclin B-dependent manner. BMC Dev Biol. 2013;13:28 pubmed publisher
Dejima K, Kleinschmit A, Takemura M, Choi P, Kinoshita Toyoda A, Toyoda H, et al. The role of Drosophila heparan sulfate 6-O-endosulfatase in sulfation compensation. J Biol Chem. 2013;288:6574-82 pubmed publisher
Pan G, Feng Y, Ambegaonkar A, Sun G, Huff M, Rauskolb C, et al. Signal transduction by the Fat cytoplasmic domain. Development. 2013;140:831-42 pubmed publisher
Kleinschmit A, Takemura M, Dejima K, Choi P, Nakato H. Drosophila heparan sulfate 6-O-endosulfatase Sulf1 facilitates wingless (Wg) protein degradation. J Biol Chem. 2013;288:5081-9 pubmed publisher
Zhang J, Liu M, Su Y, Du J, Zhu A. A targeted in vivo RNAi screen reveals deubiquitinases as new regulators of Notch signaling. G3 (Bethesda). 2012;2:1563-75 pubmed publisher
San Juán B, Andrade Zapata I, Baonza A. The bHLH factors Dpn and members of the E(spl) complex mediate the function of Notch signalling regulating cell proliferation during wing disc development. Biol Open. 2012;1:667-76 pubmed publisher
Herz H, Mohan M, Garruss A, Liang K, Takahashi Y, Mickey K, et al. Enhancer-associated H3K4 monomethylation by Trithorax-related, the Drosophila homolog of mammalian Mll3/Mll4. Genes Dev. 2012;26:2604-20 pubmed publisher
Lee J, Fischer J. Drosophila Tel2 is expressed as a translational fusion with EpsinR and is a regulator of wingless signaling. PLoS ONE. 2012;7:e46357 pubmed publisher
Birbrair A, Zhang T, Wang Z, Messi M, ENIKOLOPOV G, Mintz A, et al. Skeletal muscle neural progenitor cells exhibit properties of NG2-glia. Exp Cell Res. 2013;319:45-63 pubmed publisher
Gurudatta B, Ramos E, Corces V. The BEAF insulator regulates genes involved in cell polarity and neoplastic growth. Dev Biol. 2012;369:124-32 pubmed publisher
Pallavi S, Ho D, Hicks C, Miele L, Artavanis Tsakonas S. Notch and Mef2 synergize to promote proliferation and metastasis through JNK signal activation in Drosophila. EMBO J. 2012;31:2895-907 pubmed publisher
Mouri K, Horiuchi S, Uemura T. Cohesin controls planar cell polarity by regulating the level of the seven-pass transmembrane cadherin Flamingo. Genes Cells. 2012;17:509-24 pubmed publisher
Bribian A, Fontana X, Llorens F, Gavin R, Reina M, Garcia Verdugo J, et al. Role of the cellular prion protein in oligodendrocyte precursor cell proliferation and differentiation in the developing and adult mouse CNS. PLoS ONE. 2012;7:e33872 pubmed publisher
Koles K, Nunnari J, Korkut C, Barria R, Brewer C, Li Y, et al. Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons. J Biol Chem. 2012;287:16820-34 pubmed publisher
Aikin R, Cervantes A, D Angelo G, Ruel L, Lacas Gervais S, Schaub S, et al. A genome-wide RNAi screen identifies regulators of cholesterol-modified hedgehog secretion in Drosophila. PLoS ONE. 2012;7:e33665 pubmed publisher
Tang X, Wu Y, Belenkaya T, Huang Q, Ray L, Qu J, et al. Roles of N-glycosylation and lipidation in Wg secretion and signaling. Dev Biol. 2012;364:32-41 pubmed publisher
Wang W, Yoder J. Drosophila pupal abdomen immunohistochemistry. J Vis Exp. 2011;: pubmed publisher
Koppen T, Weckmann A, Muller S, Staubach S, Bloch W, Dohmen R, et al. Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells. Proteomics. 2011;11:4397-410 pubmed publisher
Perez L, Barrio L, Cano D, Fiuza U, Muzzopappa M, Milan M. Enhancer-PRE communication contributes to the expansion of gene expression domains in proliferating primordia. Development. 2011;138:3125-34 pubmed publisher
Dalton H, Denton D, Foot N, Ho K, Mills K, Brou C, et al. Drosophila Ndfip is a novel regulator of Notch signaling. Cell Death Differ. 2011;18:1150-60 pubmed publisher
Song H, Goetze S, Bischof J, Spichiger Haeusermann C, Kuster M, Brunner E, et al. Coop functions as a corepressor of Pangolin and antagonizes Wingless signaling. Genes Dev. 2010;24:881-6 pubmed publisher
Varelas X, Miller B, Sopko R, Song S, Gregorieff A, Fellouse F, et al. The Hippo pathway regulates Wnt/beta-catenin signaling. Dev Cell. 2010;18:579-91 pubmed publisher
Yan D, Wu Y, Feng Y, Lin S, Lin X. The core protein of glypican Dally-like determines its biphasic activity in wingless morphogen signaling. Dev Cell. 2009;17:470-81 pubmed publisher
Wright K, Tjian R. Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo. Proc Natl Acad Sci U S A. 2009;106:55-60 pubmed publisher
Singh S, Tokhunts R, Baubet V, Goetz J, Huang Z, Schilling N, et al. Sonic hedgehog mutations identified in holoprosencephaly patients can act in a dominant negative manner. Hum Genet. 2009;125:95-103 pubmed publisher
Somorjai I, Martinez Arias A. Wingless signalling alters the levels, subcellular distribution and dynamics of Armadillo and E-cadherin in third instar larval wing imaginal discs. PLoS ONE. 2008;3:e2893 pubmed publisher
Franch Marro X, Wendler F, Guidato S, Griffith J, Baena Lopez A, Itasaki N, et al. Wingless secretion requires endosome-to-Golgi retrieval of Wntless/Evi/Sprinter by the retromer complex. Nat Cell Biol. 2008;10:170-7 pubmed publisher
Chang J, Lin H, Blauwkamp T, Cadigan K. Spenito and Split ends act redundantly to promote Wingless signaling. Dev Biol. 2008;314:100-11 pubmed publisher
Geffers I, Serth K, Chapman G, Jaekel R, Schuster Gossler K, Cordes R, et al. Divergent functions and distinct localization of the Notch ligands DLL1 and DLL3 in vivo. J Cell Biol. 2007;178:465-76 pubmed
Manak J, Wen H, Van T, Andrejka L, Lipsick J. Loss of Drosophila Myb interrupts the progression of chromosome condensation. Nat Cell Biol. 2007;9:581-7 pubmed
Blitzer J, Nusse R. A critical role for endocytosis in Wnt signaling. BMC Cell Biol. 2006;7:28 pubmed
Gallet A, Ruel L, Staccini Lavenant L, Therond P. Cholesterol modification is necessary for controlled planar long-range activity of Hedgehog in Drosophila epithelia. Development. 2006;133:407-18 pubmed
Mukherjee A, Veraksa A, Bauer A, Rosse C, Camonis J, Artavanis Tsakonas S. Regulation of Notch signalling by non-visual beta-arrestin. Nat Cell Biol. 2005;7:1191-201 pubmed
Wechezak A, Coan D. Subcellular distribution of Wnt-1 at adherens junctions and actin-rich densities in endothelial cells. Exp Cell Res. 2003;288:335-43 pubmed
Foucher I, Montesinos M, Volovitch M, Prochiantz A, Trembleau A. Joint regulation of the MAP1B promoter by HNF3beta/Foxa2 and Engrailed is the result of a highly conserved mechanism for direct interaction of homeoproteins and Fox transcription factors. Development. 2003;130:1867-76 pubmed
Weidenfeld J, Shu W, Zhang L, Millar S, Morrisey E. The WNT7b promoter is regulated by TTF-1, GATA6, and Foxa2 in lung epithelium. J Biol Chem. 2002;277:21061-70 pubmed
Tanaka K, Kitagawa Y, Kadowaki T. Drosophila segment polarity gene product porcupine stimulates the posttranslational N-glycosylation of wingless in the endoplasmic reticulum. J Biol Chem. 2002;277:12816-23 pubmed
Brook W, Cohen S. Antagonistic interactions between wingless and decapentaplegic responsible for dorsal-ventral pattern in the Drosophila Leg. Science. 1996;273:1373-7 pubmed
Barry R. Scoring criteria for response latency and habituation in electrodermal research: a study in the context of the orienting response. Psychophysiology. 1990;27:94-100 pubmed
product information
Internal ID :
1770
Name :
4D4
Depositor Name :
Cohen, S.M.
Depositor Institution :
EMBL
Date Deposited :
10/7/98
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Wingless protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Chicken,Drosophila,Lizard,Mouse,Planaria
Species Tested (legacy) :
Drosophila, planaria
Initial Publication Pubmed ID :
8703069
Depositor Notes (Special Instructions) :
Antibody is specific for Drosophila Wingless protein
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Cohen, Stephen M., Wingless protein, Drosophila, MIgG1, Drosophila/Planaria/chicken/mouse, wg, Br/DWint-1/ Dint-1/ Dm Wg/ Dm-1/ WNT/Wnt, Wnt-1/ int-1/ spdl/, AB_528512, monoclonal
Antigen Molecular Weight :
Predicted: 46-51 kDa
Gene :
wg
Alternate Gene Name(s) :
Br/DWint-1/ Dint-1/ Dm Wg/ Dm-1/ WNT/Wnt, Wnt-1/ int-1/ spdl/
Uniprot ID :
P09615
Antibody Registry ID :
AB_528512
Immunogen :
Wingless (Drosophila, C-terminus a.a. 3-468); recombinant.
Clonality :
Monoclonal
Myeloma Strain :
Ag8
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
21988937 16824228 17450131 22437826 22285813 23166019 22285813 19853561 18174108 20966964 27452716 27301278 31326540 28233575
Immunofluorescence Pubmed IDs :
22285813 24335257 23275879 22432040 22563761 22580825 20412773 27301278 28233575 28808034 29530880 29670104 27095493 31332194
Western Blot Pubmed IDs :
28233575 23300081 11821428 20439429 21901833 23318637 23300081 12915124 22285813 31332194
Immunoprecipitation Pubmed IDs :
16284625 22285813 19853561
FFPE Pubmed IDs :
28233575
Gel Supershift Pubmed IDs :
12642491 11914369 19057928
Pubmed IDs :
23339195 24550732 22529900 23300081 22529900 24980505 2339192 24003085 2440318251718871 22999866 23213460 23991155 23275879 23848468 18193037 24821423 19116271 16396912 25010066 21715425 22743648 22563761
DSHB Growth Medium :
RPMI
References (legacy) :
Science 273, 1373-1377.; Development 127, 4433-4442.; Nature Cell Biol. 3(9), 816-822.; J. Histochem. Cytochem. 49(9), 1177-1182.; Cell 111, 893-904.; Genesis 32, 293-298.; Evol. Dev. 10(5), 537-545.; Dev. Biol. 350, 139-151.; Matrix Biol. 30, 258-266.; J. Biol. Chem. 288(7), 5081-5089; Development 140, 810-819; J. Genet. Genomics 41, 369-378.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US