peanut gene protein product | Developmental Studies Hybridoma Bank 4C9H4 anti-peanut product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

peanut gene protein product

catalog :

4C9H4 anti-peanut

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4C9H4 anti-peanut

reactivity :

fruit fly, common platanna

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; common platanna; 1:5; fig 4	Laird J, Pan Y, Modestou M, Yamaguchi D, Song H, Sokolov M, et al. Identification of a VxP Targeting Signal in the Flagellar Na+ /K+ -ATPase. Traffic. 2015;16:1239-53 pubmed publisher
	O Neill R, Clark D. Partial Functional Diversification of Drosophila melanogaster Septin Genes Sep2 and Sep5. G3 (Bethesda). 2016;6:1947-57 pubmed publisher
	Chougule A, Hastert M, Thomas J. Drak Is Required for Actomyosin Organization During Drosophila Cellularization. G3 (Bethesda). 2016;6:819-28 pubmed publisher
	Zaytseva O, Tenis N, Mitchell N, Kanno S, Yasui A, Heierhorst J, et al. The novel zinc finger protein dASCIZ regulates mitosis in Drosophila via an essential role in dynein light-chain expression. Genetics. 2014;196:443-53 pubmed publisher
	Goldbach P, Wong R, Beise N, Sarpal R, Trimble W, Brill J. Stabilization of the actomyosin ring enables spermatocyte cytokinesis in Drosophila. Mol Biol Cell. 2010;21:1482-93 pubmed publisher
	Shindo M, Wada H, Kaido M, Tateno M, Aigaki T, Tsuda L, et al. Dual function of Src in the maintenance of adherens junctions during tracheal epithelial morphogenesis. Development. 2008;135:1355-64 pubmed publisher
	Ji Y, Rath U, Girton J, Johansen K, Johansen J. D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut. J Neurobiol. 2005;64:157-69 pubmed
	Shih H, Hales K, Pringle J, Peifer M. Identification of septin-interacting proteins and characterization of the Smt3/SUMO-conjugation system in Drosophila. J Cell Sci. 2002;115:1259-71 pubmed
	Riparbelli M, Callaini G, Glover D, Avides M. A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila. J Cell Sci. 2002;115:913-22 pubmed
	Adam J, Pringle J, Peifer M. Evidence for functional differentiation among Drosophila septins in cytokinesis and cellularization. Mol Biol Cell. 2000;11:3123-35 pubmed
	Carmena M, Riparbelli M, Minestrini G, Tavares A, Adams R, Callaini G, et al. Drosophila polo kinase is required for cytokinesis. J Cell Biol. 1998;143:659-71 pubmed
	Field C, al Awar O, Rosenblatt J, Wong M, Alberts B, Mitchison T. A purified Drosophila septin complex forms filaments and exhibits GTPase activity. J Cell Biol. 1996;133:605-16 pubmed
	Fares H, Peifer M, Pringle J. Localization and possible functions of Drosophila septins. Mol Biol Cell. 1995;6:1843-59 pubmed
	Neufeld T, Rubin G. The Drosophila peanut gene is required for cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins. Cell. 1994;77:371-9 pubmed

product information

Internal ID :

1600

Name :

4C9H4 anti-peanut

Depositor Name :

Rubin, G.M.

Depositor Institution :

University of California-Berkeley

Date Deposited :

8/31/94

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

peanut gene protein product

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Initial Publication Pubmed ID :

8181057

Depositor Notes (Special Instructions) :

4C9H4 is an excellent marker for cytokinesis, and labels the cleavage furrow of dividing embryonic and imaginal disc cells. It recognizes the product of the peanut gene, which encodes a protein similar to the bud neck filament proteins of S. cerevisiae (CDC3, CDC10, CDC11 and CDC12).

Collections :

Cell markers,Cytoskeleton,Drosophila antigens

Search Keywords :

Rubin, G.M., peanut gene protein product, Drosophila, MIgG1, kappa light chain, Drosophila, pnut, CG8705, AB_528429, Monoclonal, Drosophila antigens/Cell markers, Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 60 kDa; Apparent: 60 kDa

Gene :

pnut

Alternate Gene Name(s) :

CG8705

Uniprot ID :

P40797

Antibody Registry ID :

AB_528429

Synonyms (Alternate Clone Names) :

4C9

Immunogen :

GST-fusion protein containing the amino-terminal 116 aa of the peanut protein.

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3x63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

amino-terminal 116 aa

Epitope Map PubMed ID :

35801

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

8181057 9813088 10982405 8590810 15818553 11884525 11870210 24336747 27172205 20237160 26818071 27146115

Western Blot Pubmed IDs :

8181057 10982405 8636235 8590810 15818553 18305002

Immunoprecipitation Pubmed IDs :

8590810 15818553

Additional Information :

Peanut is a septin

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 77, 371-379.; J. Cell Biol. 143 (3), 659-671.

more info or order :

Developmental Studies Hybridoma Bank product webpage