product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
rhodopsin 1 (Rh1 Drosophila)
catalog :
4C5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4C5
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunoprecipitation
citations: 18
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly; 1:100; fig 3
Chen K, Lin G, Haelterman N, Ho T, Li T, Li Z, et al. Loss of Frataxin induces iron toxicity, sphingolipid synthesis, and Pdk1/Mef2 activation, leading to neurodegeneration. elife. 2016;5: pubmed publisher
  • immunohistochemistry; fruit fly; 1:50; fig 6
Xu Z, Chikka M, Xia H, Ready D. Ire1 supports normal ER differentiation in developing Drosophila photoreceptors. J Cell Sci. 2016;129:921-9 pubmed publisher
  • immunohistochemistry; fruit fly; 1:200; loading ...; fig 5b
Xu Y, Wang T. CULD is required for rhodopsin and TRPL channel endocytic trafficking and survival of photoreceptor cells. J Cell Sci. 2016;129:394-405 pubmed publisher
  • immunohistochemistry; fruit fly; 1:500; fig 5
Kang K, Ryoo H, Park J, Yoon J, Kang M. A Drosophila Reporter for the Translational Activation of ATF4 Marks Stressed Cells during Development. PLoS ONE. 2015;10:e0126795 pubmed publisher
Cerny A, Altendorfer A, Schopf K, Baltner K, Maag N, Sehn E, et al. The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells. PLoS Genet. 2015;11:e1005578 pubmed publisher
Zheng L, Farrell D, Fulton R, Bagg E, Salcedo E, Manino M, et al. Analysis of Conserved Glutamate and Aspartate Residues in Drosophila Rhodopsin 1 and Their Influence on Spectral Tuning. J Biol Chem. 2015;290:21951-61 pubmed publisher
Chakrabarti P, Kolay S, Yadav S, Kumari K, Nair A, Trivedi D, et al. A dPIP5K dependent pool of phosphatidylinositol 4,5 bisphosphate (PIP2) is required for G-protein coupled signal transduction in Drosophila photoreceptors. PLoS Genet. 2015;11:e1004948 pubmed publisher
Kunduri G, Yuan C, Parthibane V, Nyswaner K, Kanwar R, Nagashima K, et al. Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors. J Cell Biol. 2014;206:79-95 pubmed publisher
Wang S, Tan K, Agosto M, Xiong B, Yamamoto S, Sandoval H, et al. The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration. PLoS Biol. 2014;12:e1001847 pubmed publisher
Satoh T, Inagaki T, Liu Z, Watanabe R, Satoh A. GPI biosynthesis is essential for rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors. Development. 2013;140:385-94 pubmed publisher
Elsaesser R, Kalra D, Li R, Montell C. Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2. Proc Natl Acad Sci U S A. 2010;107:4740-5 pubmed publisher
Yano H, Yamamoto Hino M, Goto S. Spatial and temporal regulation of glycosylation during Drosophila eye development. Cell Tissue Res. 2009;336:137-47 pubmed publisher
Li B, Satoh A, Ready D. Myosin V, Rab11, and dRip11 direct apical secretion and cellular morphogenesis in developing Drosophila photoreceptors. J Cell Biol. 2007;177:659-69 pubmed
Vishnu S, Hertenstein A, Betschinger J, Knoblich J, Gert de Couet H, Fischbach K. The adaptor protein X11Lalpha/Dmint1 interacts with the PDZ-binding domain of the cell recognition protein Rst in Drosophila. Dev Biol. 2006;289:296-307 pubmed
Beronja S, Laprise P, Papoulas O, Pellikka M, Sisson J, Tepass U. Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells. J Cell Biol. 2005;169:635-46 pubmed
LaLonde M, Janssens H, Rosenbaum E, Choi S, Gergen J, Colley N, et al. Regulation of phototransduction responsiveness and retinal degeneration by a phospholipase D-generated signaling lipid. J Cell Biol. 2005;169:471-9 pubmed
Sang T, Ready D. Eyes closed, a Drosophila p47 homolog, is essential for photoreceptor morphogenesis. Development. 2002;129:143-54 pubmed
Lee S, Montell C. Regulation of the rhodopsin protein phosphatase, RDGC, through interaction with calmodulin. Neuron. 2001;32:1097-106 pubmed
product information
Internal ID :
2102
Name :
4C5
Depositor Name :
de Couet, H.G. / Tanimura, T.
Depositor Institution :
University of Hawaii
Date Deposited :
1/18/96
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
rhodopsin 1 (Rh1 Drosophila)
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
no known cross-reactivity to date
Initial Publication Pubmed ID :
16380111
Collections :
Drosophila antigens
Search Keywords :
de Couet, Heinz Gert / Tanimura, Teiichi, Rhodopsin Drosophila, Drosophila, MIgG1, Drosophila, Dm Rh1; DMELRH1; Dmel Nina E; opsin; Rh1/ninaE; rhodopsin; Rhodopsin-1C-beta, AB_528451, monoclonal
Antigen Molecular Weight :
Predicted: 39 kDa,
Gene :
ninaE
Alternate Gene Name(s) :
Dm Rh1; DMELRH1; Dmel Nina E; opsin; Rh1/ninaE; rhodopsin; Rhodopsin-1C-beta
Uniprot ID :
P06002
Antibody Registry ID :
AB_528451
Immunogen :
Rhodopsin (1-6) Drosophila melanogaster; recombinant
Clonality :
Monoclonal
Myeloma Strain :
NA
Epitope Mapped :
No
Recommended Applications :
Immunohistochemistry,Immunoprecipitation,Western Blot
Immunohistochemistry Pubmed IDs :
20176938 19214580 16380111 11782408 25633995
Immunofluorescence Pubmed IDs :
25002678 20176938
Western Blot Pubmed IDs :
23250212 25002678 25633995
Immunoprecipitation Pubmed IDs :
20176938 25002678 20176938
Pubmed IDs :
16380111 23250212 15897260 11754840 25633995 20176938 19214580 25002678
DSHB Growth Medium :
RPMI
References (legacy) :
Eur. J. Cell Biol. 44, 50-56.; Cell 67, 255-263.; EMBO 13, 4886-4895.; Proc. Natl. Acad. Sci. USA 92, 3070-3074.; J. Biol. Chem. 287(17), 13911-13918.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US