fibronectin, type III repeat 10, function blocking | Developmental Studies Hybridoma Bank 4B12 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

fibronectin, type III repeat 10, function blocking

catalog :

4B12

clonality :

monoclonal

host :

house mouse

conjugate :

nonconjugated

clone name :

4B12

reactivity :

Xenopus laevis

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Reference
Spicer E, Suckert C, Al Attar H, Marsden M. Integrin alpha5beta1 function is regulated by XGIPC/kermit2 mediated endocytosis during Xenopus laevis gastrulation. PLoS ONE. 2010;5:e10665 pubmed publisher
Rozario T, Dzamba B, Weber G, Davidson L, DeSimone D. The physical state of fibronectin matrix differentially regulates morphogenetic movements in vivo. Dev Biol. 2009;327:386-98 pubmed publisher
Marsden M, DeSimone D. Integrin-ECM interactions regulate cadherin-dependent cell adhesion and are required for convergent extension in Xenopus. Curr Biol. 2003;13:1182-91 pubmed
Na J, Marsden M, DeSimone D. Differential regulation of cell adhesive functions by integrin alpha subunit cytoplasmic tails in vivo. J Cell Sci. 2003;116:2333-43 pubmed
Marsden M, DeSimone D. Regulation of cell polarity, radial intercalation and epiboly in Xenopus: novel roles for integrin and fibronectin. Development. 2001;128:3635-47 pubmed
Ramos J, Whittaker C, DeSimone D. Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation. Development. 1996;122:2873-83 pubmed

product information

Internal ID :

23896

Name :

4B12

Depositor Name :

DeSimone, D.W.

Depositor Institution :

University of Virginia

Depositor Website :

https://med.virginia.edu/cell-biology/lab-snap-shot/desimone-lab/

Date Deposited :

6/23/15

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

fibronectin, type III repeat 10, function blocking

Antigen Species :

Xenopus laevis

Host Species :

mouse

Isotype :

MIgG1

Positive Tested Species Reactivity :

Xenopus laevis

Initial Publication Pubmed ID :

8787760

Depositor Notes (Special Instructions) :

This antibody blocks cell attachment to fibronectin.

Collections :

Extracellular matrix proteins,Xenopus

Search Keywords :

DeSimone, D.W., Fibronectin, Xenopus laevis, MIgG1, Xenopus laevis, fn1, FN, cig, finc, lets, msf, , Monoclonal, Extracellular matrix proteins/Xenopus, Function blocking

Antigen Molecular Weight :

Predicted (multiple isoforms):up to 270 kDa; Apparent: 220 kDa (multiple isoforms)

Gene :

fn1

Alternate Gene Name(s) :

FN, cig, finc, lets, msf

Uniprot ID :

Q91740

Antibody Registry ID :

AB_2721945

Immunogen :

Recombinant GST/FN (type III repeats 9 to 11) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/O-Ag 14

Epitope Mapped :

Yes

Epitope Location or Sequence :

Type III repeat 10

Epitope Map PubMed ID :

8787760

Recommended Applications :

Function Blocking,Immunofluorescence,Western Blot

Immunofluorescence Pubmed IDs :

20498857

Western Blot Pubmed IDs :

8787760 20498857

Function Blocking Pubmed IDs :

8787760 11566866 12867028 12711704

Pubmed IDs :

19138684

Additional Information :

RRID:AB_2721945

DSHB Growth Medium :

Iscove's

more info or order :

Developmental Studies Hybridoma Bank product webpage