major sperm protein (MSP) | Developmental Studies Hybridoma Bank 4A5 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

major sperm protein (MSP)

catalog :

4A5

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4A5

reactivity :

Caenorhabditis elegans

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Reference
Conine C, Moresco J, Gu W, Shirayama M, Conte D, Yates J, et al. Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans. Cell. 2013;155:1532-44 pubmed publisher
Kim S, Govindan J, Tu Z, Greenstein D. SACY-1 DEAD-Box helicase links the somatic control of oocyte meiotic maturation to the sperm-to-oocyte switch and gamete maintenance in Caenorhabditis elegans. Genetics. 2012;192:905-28 pubmed publisher
Wu J, Go A, Samson M, Cintra T, Mirsoian S, Wu T, et al. Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans. Genetics. 2012;190:143-57 pubmed publisher
Fraire Zamora J, Broitman Maduro G, Maduro M, Cardullo R. Evidence for phosphorylation in the MSP cytoskeletal filaments of amoeboid spermatozoa. Int J Biochem Mol Biol. 2011;2:263-73 pubmed
Sasagawa Y, Yamanaka K, Saito Sasagawa Y, Ogura T. Caenorhabditis elegans UBX cofactors for CDC-48/p97 control spermatogenesis. Genes Cells. 2010;15:1201-15 pubmed publisher
Heger P, Kroiher M, Ndifon N, Schierenberg E. Conservation of MAP kinase activity and MSP genes in parthenogenetic nematodes. BMC Dev Biol. 2010;10:51 pubmed publisher
Sasagawa Y, Otani M, Higashitani N, Higashitani A, Sato K, Ogura T, et al. Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner. J Cell Sci. 2009;122:3663-72 pubmed publisher
Kosinski M, McDonald K, Schwartz J, Yamamoto I, Greenstein D. C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes. Development. 2005;132:3357-69 pubmed

product information

Internal ID :

3730

Name :

4A5

Depositor Name :

Greenstein, D.

Depositor Institution :

University of Minnesota

Date Deposited :

5/4/06

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

major sperm protein (MSP)

Antigen Species :

nematode

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

C. elegans,Nematode

Species Tested (legacy) :

nematodes

Initial Publication Pubmed ID :

15975936

Collections :

C. elegans,Cell signaling,Cytoskeleton

Search Keywords :

Greenstein, D., major sperm protein (MSP), nematode, MIgG1, kappa light chain, C. elegans/nematode, msp, , AB_760348, monoclonal, C. elegans/Cell signaling/Cytoskeletal elements, Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 14 kDa; Apparent: 15 kDa

Gene :

msp

Uniprot ID :

P53017

Antibody Registry ID :

AB_760348

Immunogen :

peptide (CGG-REWFQGDGMVRRKNLPIEYNP, aa 106-126 of MSP C-terminal) coupled to KLH

Immunogen Sequence :

Peptide

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

C terminal (aa 106-126)

Epitope Map PubMed ID :

15975936

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

27468638

Immunofluorescence Pubmed IDs :

15975936 20478028 22042574 22003439 19773360 24360276 20977550 22887816

Western Blot Pubmed IDs :

15975936 20478028 22042574 22003439

Additional Information :

In C. elegans, MSP is encoded by a large multigene family. The MSP epitope recognized by 4A5 withstands aldehydye fixation, osmium post0fix and resin thin sesctioning.

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 132, 3357-3369.; BMC Dev. Biol. 10:51.

more info or order :

Developmental Studies Hybridoma Bank product webpage