product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
islet-1 homeobox
catalog :
40.3A4
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
40.3A4
reactivity :
human, mouse, rat, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 31
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:10; fig 3a
Cortes D, Robledo Arratia Y, Hernández Martinez R, Escobedo Ávila I, Bargas J, Velasco I. Transgenic GDNF Positively Influences Proliferation, Differentiation, Maturation and Survival of Motor Neurons Produced from Mouse Embryonic Stem Cells. Front Cell Neurosci. 2016;10:217 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:300; fig 2
Abdellatif A, Ogata K, Kudo T, Xiafukaiti G, Chang Y, Katoh M, et al. Role of large MAF transcription factors in the mouse endocrine pancreas. Exp Anim. 2015;64:305-12 pubmed publisher
  • EMSA; mouse
Ediger B, Du A, Liu J, Hunter C, Walp E, Schug J, et al. Islet-1 Is essential for pancreatic ?-cell function. Diabetes. 2014;63:4206-17 pubmed publisher
Angueyra J, Kindt K. Leveraging Zebrafish to Study Retinal Degenerations. Front Cell Dev Biol. 2018;6:110 pubmed publisher
Parchem R, Moore N, Fish J, Parchem J, Braga T, Shenoy A, et al. miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability. Cell Rep. 2015;12:760-73 pubmed publisher
Cambier L, Plate M, Sucov H, Pashmforoush M. Nkx2-5 regulates cardiac growth through modulation of Wnt signaling by R-spondin3. Development. 2014;141:2959-71 pubmed publisher
Ocorr K, Vogler G, Bodmer R. Methods to assess Drosophila heart development, function and aging. Methods. 2014;68:265-72 pubmed publisher
Yan X, Lin J, Talabattula V, Mußmann C, Yang F, Wree A, et al. ADAM10 negatively regulates neuronal differentiation during spinal cord development. PLoS ONE. 2014;9:e84617 pubmed publisher
Koo J, Zhou X, Moschiano E, de Peralta Venturina M, Mertens R, Dhall D. The immunohistochemical expression of islet 1 and PAX8 by rectal neuroendocrine tumors should be taken into account in the differential diagnosis of metastatic neuroendocrine tumors of unknown primary origin. Endocr Pathol. 2013;24:184-90 pubmed publisher
Zmojdzian M, Jagla K. Tailup plays multiple roles during cardiac outflow assembly in Drosophila. Cell Tissue Res. 2013;354:639-45 pubmed
Su H, Wang L, Cai J, Yuan Q, Yang X, Yao X, et al. Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle. Stem Cell Res. 2013;11:529-39 pubmed publisher
Kim K, Nam J, Mukouyama Y, Kawamoto S. Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development. J Cell Biol. 2013;200:443-58 pubmed publisher
Liu C, Lin C, Whitaker D, Bakeri H, Bulgakov O, Liu P, et al. Prickle1 is expressed in distinct cell populations of the central nervous system and contributes to neuronal morphogenesis. Hum Mol Genet. 2013;22:2234-46 pubmed publisher
Koo J, Mertens R, Mirocha J, Wang H, Dhall D. Value of Islet 1 and PAX8 in identifying metastatic neuroendocrine tumors of pancreatic origin. Mod Pathol. 2012;25:893-901 pubmed publisher
Liu C, Bakeri H, Li T, Swaroop A. Regulation of retinal progenitor expansion by Frizzled receptors: implications for microphthalmia and retinal coloboma. Hum Mol Genet. 2012;21:1848-60 pubmed publisher
Mullen R, Park S, Rhodes S. A distal modular enhancer complex acts to control pituitary- and nervous system-specific expression of the LHX3 regulatory gene. Mol Endocrinol. 2012;26:308-19 pubmed publisher
de Navascués J, Modolell J. The pronotum LIM-HD gene tailup is both a positive and a negative regulator of the proneural genes achaete and scute of Drosophila. Mech Dev. 2010;127:393-406 pubmed publisher
Du A, Hunter C, Murray J, Noble D, Cai C, Evans S, et al. Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas. Diabetes. 2009;58:2059-69 pubmed publisher
Diensthuber M, Oshima K, Heller S. Stem/progenitor cells derived from the cochlear sensory epithelium give rise to spheres with distinct morphologies and features. J Assoc Res Otolaryngol. 2009;10:173-90 pubmed publisher
Hari P, Deshpande M, Sharma N, Rajadhyaksha N, Ramkumar N, Kimura K, et al. Chip is required for posteclosion behavior in Drosophila. J Neurosci. 2008;28:9145-50 pubmed publisher
Schmitt A, Riniker F, Anlauf M, Schmid S, Soltermann A, Moch H, et al. Islet 1 (Isl1) expression is a reliable marker for pancreatic endocrine tumors and their metastases. Am J Surg Pathol. 2008;32:420-5 pubmed publisher
de Navascués J, Modolell J. tailup, a LIM-HD gene, and Iro-C cooperate in Drosophila dorsal mesothorax specification. Development. 2007;134:1779-88 pubmed
Odani N, Pfaff S, Nakamura H, Funahashi J. Cloning and developmental expression of a chick G-protein-coupled receptor SCGPR1. Gene Expr Patterns. 2007;7:375-80 pubmed
Biryukova I, Heitzler P. The Drosophila LIM-homeo domain protein Islet antagonizes pro-neural cell specification in the peripheral nervous system. Dev Biol. 2005;288:559-70 pubmed
Li H, Corrales C, Wang Z, Zhao Y, Wang Y, Liu H, et al. BMP4 signaling is involved in the generation of inner ear sensory epithelia. BMC Dev Biol. 2005;5:16 pubmed
Plachta N, Bibel M, Tucker K, Barde Y. Developmental potential of defined neural progenitors derived from mouse embryonic stem cells. Development. 2004;131:5449-56 pubmed
Li H, Liu H, Sage C, Huang M, Chen Z, Heller S. Islet-1 expression in the developing chicken inner ear. J Comp Neurol. 2004;477:1-10 pubmed
Watanabe Y, Toyoda R, Nakamura H. Navigation of trochlear motor axons along the midbrain-hindbrain boundary by neuropilin 2. Development. 2004;131:681-92 pubmed
Robb S, SANCHEZ ALVARADO A. Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy. Genesis. 2002;32:293-8 pubmed
Thor S, Ericson J, Brannstrom T, Edlund T. The homeodomain LIM protein Isl-1 is expressed in subsets of neurons and endocrine cells in the adult rat. Neuron. 1991;7:881-9 pubmed
Ericson J, Thor S, Edlund T, Jessell T, Yamada T. Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1. Science. 1992;256:1555-60 pubmed
product information
Internal ID :
2380
Name :
40.3A4
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
HHMI/Columbia University
Date Deposited :
8/27/98
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
islet-1 homeobox
Antigen Species :
rat
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Chicken,Drosophila,Human,Mouse,Planaria,Rat
Species Tested (legacy) :
rat, mouse, chick, Drosophila planaria and human
Initial Publication Pubmed ID :
1350865
Depositor Notes (Special Instructions) :
The 40.3A4 monoclonal antibody is often combined with monoclonal antibodies 39.3F7, 39.4D5, and 40.2D6 to create a efficient cocktail for ChIP and/or gel shift [PMID 19502415].
Collections :
Cell markers,Drosophila antigens,Human,Neurodevelopment,Transcription factors
Search Keywords :
Jessell, Thomas M / Brenner-Morton, Susan, islet-1 homeobox, Rat, MIgG1, Human/Rat/Mouse/Chicken/Drosophila/Planaria, Isl1, Isl-1; isl-1=homeobox, AB_528313, monoclonal
Antigen Molecular Weight :
Predicted: 39kDa; Apparent: 39kDa
Gene :
Isl1
Alternate Gene Name(s) :
Isl-1; isl-1=homeobox
Uniprot ID :
P61374
Antibody Registry ID :
AB_528313
Immunogen :
C-terminal of rat Islet-1 (aa 178-349); recombinant .
Immunogen Sequence :
Recombinant C-terminal of rat Islet-1 (aa 178-349)
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
Yes
Epitope Map PubMed ID :
1350865 1764243
Recommended Applications :
Chromatin Immunoprecipitation,FFPE,Gel Supershift,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
14729576 18300808 24037217 22388755
Immunofluorescence Pubmed IDs :
1967300211493563 11948917 17409113 24404179 14729576 16259974 23578695 18784295 23797334 19247714 17251065 16107213 15281076 20580820 23420014 23420872 25053429 22228100
Western Blot Pubmed IDs :
16259974
Chromatin Immunoprecipitation Pubmed IDs :
22194342 19502415
FFPE Pubmed IDs :
18300808 24037217 22388755
Gel Supershift Pubmed IDs :
25028525 19502415
Pubmed IDs :
15469972 24727147
DSHB Growth Medium :
Iscove's
References (legacy) :
Genesis 32, 293-298.; Am. J. Surg. Pathol. 32(3), 420-425.; Endocr. Pathol. 24, 184-190.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US