product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Islet-1 homeobox
catalog :
40.2D6
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
40.2D6
reactivity :
African green monkey, slender lungfish, human, mouse, rat, chicken, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, FACS, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
citations: 113
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; rat; fig 2f
  • immunohistochemistry knockout validation; human; fig 1d
  • immunohistochemistry knockout validation; African green monkey; loading ...; fig s6a
  • immunohistochemistry - frozen section; African green monkey; loading ...; fig s6a
Yamasaki S, Tu H, Matsuyama T, Horiuchi M, Hashiguchi T, Sho J, et al. A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. iScience. 2022;25:103657 pubmed publisher
  • immunohistochemistry; mouse; 1:30; loading ...; fig 2d
Yusifov E, Dumoulin A, Stoeckli E. Investigating Primary Cilia during Peripheral Nervous System Formation. Int J Mol Sci. 2021;22: pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...
Brodie Kommit J, Clark B, Shi Q, Shiau F, Kim D, Langel J, et al. Atoh7-independent specification of retinal ganglion cell identity. Sci Adv. 2021;7: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1c
Nickolls A, Lee M, Espinoza D, Szczot M, Lam R, Wang Q, et al. Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells. Cell Rep. 2020;30:932-946.e7 pubmed publisher
  • western blot; slender lungfish; 1:500; loading ...; fig 2
L pez J, Morona R, Moreno N, Lozano D, Jim nez S, Gonz lez A. Pax6 expression highlights regional organization in the adult brain of lungfishes, the closest living relatives of land vertebrates. J Comp Neurol. 2020;528:135-159 pubmed publisher
  • immunocytochemistry; mouse; 1:50; loading ...; fig s5d
Oprişoreanu A, Smith H, Arya S, Webster R, Zhong Z, Wehner D, et al. Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation. Cell Rep. 2019;29:1082-1098.e10 pubmed publisher
  • immunohistochemistry; mouse; 1:50; loading ...; fig 1d
Su T, Liu H, Zhang D, Xu G, Liu J, Evans S, et al. LIM homeodomain transcription factor Isl1 affects urethral epithelium differentiation and apoptosis via Shh. Cell Death Dis. 2019;10:713 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:100; loading ...; fig 5b
Schick E, McCaffery S, Keblish E, Thakurdin C, Emerson M. Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina. Sci Rep. 2019;9:9358 pubmed publisher
  • immunohistochemistry; human; 1:100; loading ...; fig 6b
Zhang C, Yu W, Hoshino A, Huang J, Rieke F, Reh T, et al. Development of ON and OFF cholinergic amacrine cells in the human fetal retina. J Comp Neurol. 2019;527:174-186 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 1b
Himmels P, Paredes I, Adler H, Karakatsani A, Luck R, Marti H, et al. Motor neurons control blood vessel patterning in the developing spinal cord. Nat Commun. 2017;8:14583 pubmed publisher
  • immunohistochemistry; mouse; 1:400; loading ...; fig s9
Espinosa Medina I, Saha O, Boismoreau F, Chettouh Z, Rossi F, Richardson W, et al. The sacral autonomic outflow is sympathetic. Science. 2016;354:893-897 pubmed
  • immunocytochemistry; mouse; 1:10; fig 3a
Cortes D, Robledo Arratia Y, Hernández Martinez R, Escobedo Ávila I, Bargas J, Velasco I. Transgenic GDNF Positively Influences Proliferation, Differentiation, Maturation and Survival of Motor Neurons Produced from Mouse Embryonic Stem Cells. Front Cell Neurosci. 2016;10:217 pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; 1:100; fig 2
Chen T, Yu Y, Hu C, Schachner M. L1.2, the zebrafish paralog of L1.1 and ortholog of the mammalian cell adhesion molecule L1 contributes to spinal cord regeneration in adult zebrafish. Restor Neurol Neurosci. 2016;34:325-35 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 4
Preuße K, Tveriakhina L, Schuster Gossler K, Gaspar C, Rosa A, Henrique D, et al. Context-Dependent Functional Divergence of the Notch Ligands DLL1 and DLL4 In Vivo. PLoS Genet. 2015;11:e1005328 pubmed publisher
  • immunohistochemistry; human; 1:200; fig 1
Hoeber J, Trolle C, König N, Du Z, Gallo A, Hermans E, et al. Human Embryonic Stem Cell-Derived Progenitors Assist Functional Sensory Axon Regeneration after Dorsal Root Avulsion Injury. Sci Rep. 2015;5:10666 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig 4m-o
Gay M, Valenta T, Herr P, Paratore Hari L, Basler K, Sommer L. Distinct adhesion-independent functions of β-catenin control stage-specific sensory neurogenesis and proliferation. BMC Biol. 2015;13:24 pubmed publisher
  • immunohistochemistry; mouse; 1:750
Miller N, Feng Z, Edens B, Yang B, Shi H, Sze C, et al. Non-aggregating tau phosphorylation by cyclin-dependent kinase 5 contributes to motor neuron degeneration in spinal muscular atrophy. J Neurosci. 2015;35:6038-50 pubmed publisher
  • immunohistochemistry; mouse; fig s5
Eisner A, Pazyra Murphy M, Durresi E, Zhou P, Zhao X, Chadwick E, et al. The Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis. Dev Cell. 2015;33:22-35 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:20; fig 1
Holzmann J, Hennchen M, Rohrer H. Prox1 identifies proliferating neuroblasts and nascent neurons during neurogenesis in sympathetic ganglia. Dev Neurobiol. 2015;75:1352-67 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:600; fig 4
Gergics P, Brinkmeier M, Camper S. Lhx4 deficiency: increased cyclin-dependent kinase inhibitor expression and pituitary hypoplasia. Mol Endocrinol. 2015;29:597-612 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:300; fig 2
Karunakaran D, Chhaya N, Lemoine C, Congdon S, Black A, Kanadia R. Loss of citron kinase affects a subset of progenitor cells that alters late but not early neurogenesis in the developing rat retina. Invest Ophthalmol Vis Sci. 2015;56:787-98 pubmed publisher
  • western blot; mouse; 1:200
Blanchard J, Eade K, Szucs A, Lo Sardo V, Tsunemoto R, Williams D, et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci. 2015;18:25-35 pubmed publisher
  • immunohistochemistry; human; 1:200; fig 2e
Johnson Kerner B, Ahmad F, Diaz A, Greene J, Gray S, Samulski R, et al. Intermediate filament protein accumulation in motor neurons derived from giant axonal neuropathy iPSCs rescued by restoration of gigaxonin. Hum Mol Genet. 2015;24:1420-31 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000
  • EMSA; mouse
Ediger B, Du A, Liu J, Hunter C, Walp E, Schug J, et al. Islet-1 Is essential for pancreatic ?-cell function. Diabetes. 2014;63:4206-17 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 3
Veleri S, Manjunath S, Fariss R, May Simera H, Brooks M, Foskett T, et al. Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis. Nat Commun. 2014;5:4207 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse
Li Y, Pan J, Wei C, Chen J, Liu Y, Liu J, et al. LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3. BMC Biol. 2014;12:25 pubmed publisher
  • immunohistochemistry; mouse; fig 2
Le Bras B, Fréal A, Czarnecki A, Legendre P, Bullier E, Komada M, et al. In vivo assembly of the axon initial segment in motor neurons. Brain Struct Funct. 2014;219:1433-50 pubmed publisher
Kim B, Jeong Y, Wong M, Martin L. DNA damage accumulates and responses are engaged in human ALS brain and spinal motor neurons and DNA repair is activatable in iPSC-derived motor neurons with SOD1 mutations. Acta Neuropathol Commun. 2020;8:7 pubmed publisher
Wan Y, Wei Z, Looger L, Koyama M, Druckmann S, Keller P. Single-Cell Reconstruction of Emerging Population Activity in an Entire Developing Circuit. Cell. 2019;179:355-372.e23 pubmed publisher
Lin C, Yoshida M, Li L, Ikenaka A, Oshima S, Nakagawa K, et al. iPSC-derived functional human neuromuscular junctions model the pathophysiology of neuromuscular diseases. JCI Insight. 2019;4: pubmed publisher
Mori S, Sakakura E, Tsunekawa Y, Hagiwara M, Suzuki T, Eiraku M. Self-organized formation of developing appendages from murine pluripotent stem cells. Nat Commun. 2019;10:3802 pubmed publisher
Jacquier A, Risson V, Schaeffer L. Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons. J Vis Exp. 2019;: pubmed publisher
Eley L, Alqahtani A, MacGrogan D, Richardson R, Murphy L, Salguero Jimenez A, et al. A novel source of arterial valve cells linked to bicuspid aortic valve without raphe in mice. elife. 2018;7: pubmed publisher
Berg E, Bertuzzi M, Ampatzis K. Complementary expression of calcium binding proteins delineates the functional organization of the locomotor network. Brain Struct Funct. 2018;223:2181-2196 pubmed publisher
Angelo J, Tremblay K. Identification and fate mapping of the pancreatic mesenchyme. Dev Biol. 2018;435:15-25 pubmed publisher
Bandín S, Morona R, González A. Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis. Front Neuroanat. 2015;9:107 pubmed publisher
Sato S, Yajima H, Furuta Y, Ikeda K, Kawakami K. Activation of Six1 Expression in Vertebrate Sensory Neurons. PLoS ONE. 2015;10:e0136666 pubmed publisher
Kelder T, Vicente Steijn R, Harryvan T, Kosmidis G, Gittenberger de Groot A, Poelmann R, et al. The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?. J Cell Mol Med. 2015;19:1375-89 pubmed publisher
Terry N, Walp E, Lee R, Kaestner K, MAY C. Impaired enteroendocrine development in intestinal-specific Islet1 mouse mutants causes impaired glucose homeostasis. Am J Physiol Gastrointest Liver Physiol. 2014;307:G979-91 pubmed publisher
Maeda M, Harris A, Kingham B, Lumpkin C, Opdenaker L, McCahan S, et al. Transcriptome profiling of spinal muscular atrophy motor neurons derived from mouse embryonic stem cells. PLoS ONE. 2014;9:e106818 pubmed publisher
Yajima H, Suzuki M, Ochi H, Ikeda K, Sato S, Yamamura K, et al. Six1 is a key regulator of the developmental and evolutionary architecture of sensory neurons in craniates. BMC Biol. 2014;12:40 pubmed publisher
Wu C, Hooper R, Han K, Taneyhill L. Migratory neural crest cell ?N-catenin impacts chick trigeminal ganglia formation. Dev Biol. 2014;392:295-307 pubmed publisher
Rasheed V, Sreekanth S, Dhanesh S, Divya M, Divya T, Akhila P, et al. Developmental wave of Brn3b expression leading to RGC fate specification is synergistically maintained by miR-23a and miR-374. Dev Neurobiol. 2014;74:1155-71 pubmed publisher
Makarev E, Gorivodsky M. Islet1 and its co-factor Ldb1 are expressed in quiescent cells of mouse intestinal epithelium. PLoS ONE. 2014;9:e95256 pubmed publisher
Duval N, Daubas P, Bourcier de Carbon C, St Cloment C, Tinevez J, Lopes M, et al. Msx1 and Msx2 act as essential activators of Atoh1 expression in the murine spinal cord. Development. 2014;141:1726-36 pubmed publisher
Sunnen C, Simonet J, Marsh E, Golden J. Arx is required for specification of the zona incerta and reticular nucleus of the thalamus. J Neuropathol Exp Neurol. 2014;73:253-61 pubmed publisher
Regadas I, Matos M, Monteiro F, Gomez Skarmeta J, Lima D, Bessa J, et al. Several cis-regulatory elements control mRNA stability, translation efficiency, and expression pattern of Prrxl1 (paired related homeobox protein-like 1). J Biol Chem. 2013;288:36285-301 pubmed publisher
Matsushita F, Kameyama T, Kadokawa Y, Marunouchi T. Spatiotemporal expression pattern of Myt/NZF family zinc finger transcription factors during mouse nervous system development. Dev Dyn. 2014;243:588-600 pubmed publisher
Witman N, Heigwer J, Thaler B, Lui W, Morrison J. miR-128 regulates non-myocyte hyperplasia, deposition of extracellular matrix and Islet1 expression during newt cardiac regeneration. Dev Biol. 2013;383:253-63 pubmed publisher
Meziane H, Fraulob V, Riet F, Krezel W, Selloum M, Geffarth M, et al. The homeodomain factor Gbx1 is required for locomotion and cell specification in the dorsal spinal cord. Peerj. 2013;1:e142 pubmed publisher
Wei J, Hanna T, Suda N, Karsenty G, Ducy P. Osteocalcin promotes ?-cell proliferation during development and adulthood through Gprc6a. Diabetes. 2014;63:1021-31 pubmed publisher
Kobayashi N, Homma S, Okada T, Masuda T, Sato N, Nishiyama K, et al. Elucidation of target muscle and detailed development of dorsal motor neurons in chick embryo spinal cord. J Comp Neurol. 2013;521:2987-3002 pubmed publisher
Kim H, Woo H, Ryu J, Bok J, Kim J, Choi S, et al. Conditional deletion of pten leads to defects in nerve innervation and neuronal survival in inner ear development. PLoS ONE. 2013;8:e55609 pubmed publisher
Xu J, Nonogaki M, Madhira R, Ma H, Hermanson O, Kioussi C, et al. Population-specific regulation of Chmp2b by Lbx1 during onset of synaptogenesis in lateral association interneurons. PLoS ONE. 2012;7:e48573 pubmed publisher
Miyazaki S, Tashiro F, Fujikura J, Yamato E, Miyazaki J. Acinar-to-ductal metaplasia induced by adenovirus-mediated pancreatic expression of Isl1. PLoS ONE. 2012;7:e47536 pubmed publisher
Yang J, Mumaw J, Liu Y, Stice S, West F. SSEA4-positive pig induced pluripotent stem cells are primed for differentiation into neural cells. Cell Transplant. 2013;22:945-59 pubmed publisher
Agathocleous M, Love N, Randlett O, Harris J, Liu J, Murray A, et al. Metabolic differentiation in the embryonic retina. Nat Cell Biol. 2012;14:859-64 pubmed publisher
Chambers S, Qi Y, Mica Y, Lee G, Zhang X, Niu L, et al. Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors. Nat Biotechnol. 2012;30:715-20 pubmed publisher
Lopez J, Perlado J, Morona R, Northcutt R, González A. Neuroanatomical organization of the cholinergic system in the central nervous system of a basal actinopterygian fish, the senegal bichir Polypterus senegalus. J Comp Neurol. 2013;521:24-49 pubmed publisher
Zhong Z, Ohnmacht J, Reimer M, Bach I, Becker T, Becker C. Chondrolectin mediates growth cone interactions of motor axons with an intermediate target. J Neurosci. 2012;32:4426-39 pubmed publisher
Mullen R, Park S, Rhodes S. A distal modular enhancer complex acts to control pituitary- and nervous system-specific expression of the LHX3 regulatory gene. Mol Endocrinol. 2012;26:308-19 pubmed publisher
Lai H, Klisch T, Roberts R, Zoghbi H, Johnson J. In vivo neuronal subtype-specific targets of Atoh1 (Math1) in dorsal spinal cord. J Neurosci. 2011;31:10859-71 pubmed publisher
Kim S, Rane S. The Cdk4-E2f1 pathway regulates early pancreas development by targeting Pdx1+ progenitors and Ngn3+ endocrine precursors. Development. 2011;138:1903-12 pubmed publisher
Liu J, Hunter C, Du A, Ediger B, Walp E, Murray J, et al. Islet-1 regulates Arx transcription during pancreatic islet alpha-cell development. J Biol Chem. 2011;286:15352-60 pubmed publisher
Rigato C, Buckinx R, Le Corronc H, Rigo J, Legendre P. Pattern of invasion of the embryonic mouse spinal cord by microglial cells at the time of the onset of functional neuronal networks. Glia. 2011;59:675-95 pubmed publisher
Boulting G, Kiskinis E, Croft G, Amoroso M, Oakley D, Wainger B, et al. A functionally characterized test set of human induced pluripotent stem cells. Nat Biotechnol. 2011;29:279-86 pubmed publisher
Alfakir M, Dawe N, Eyre R, Tyson Capper A, Britton K, Robson S, et al. The temporal and spatial expression patterns of ABCG2 in the developing human heart. Int J Cardiol. 2012;156:133-8 pubmed publisher
Mavromatakis Y, Lin W, Metzakopian E, Ferri A, Yan C, Sasaki H, et al. Foxa1 and Foxa2 positively and negatively regulate Shh signalling to specify ventral midbrain progenitor identity. Mech Dev. 2011;128:90-103 pubmed publisher
Wu Y, Luo H, Liu J, Kang D, McNeilly A, Cui S. LIM homeodomain transcription factor Isl-1 enhances follicle stimulating hormone-beta and luteinizing hormone-beta gene expression and mediates the activation of leptin on gonadotropin synthesis. Endocrinology. 2010;151:4787-800 pubmed publisher
Bejarano Escobar R, Blasco M, DeGrip W, Oyola Velasco J, Martin Partido G, Francisco Morcillo J. Eye development and retinal differentiation in an altricial fish species, the senegalese sole (Solea senegalensis, Kaup 1858). J Exp Zool B Mol Dev Evol. 2010;314:580-605 pubmed publisher
Ritchey E, Bongini R, Code K, Zelinka C, Petersen Jones S, Fischer A. The pattern of expression of guanine nucleotide-binding protein beta3 in the retina is conserved across vertebrate species. Neuroscience. 2010;169:1376-91 pubmed publisher
Enjin A, Rabe N, Nakanishi S, Vallstedt A, Gezelius H, Memic F, et al. Identification of novel spinal cholinergic genetic subtypes disclose Chodl and Pitx2 as markers for fast motor neurons and partition cells. J Comp Neurol. 2010;518:2284-304 pubmed publisher
Morillo S, Escoll P, de la Hera A, Frade J. Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor. Proc Natl Acad Sci U S A. 2010;107:109-14 pubmed publisher
Goldstein R. Transplantation of human embryonic stem cells and derivatives to the chick embryo. Methods Mol Biol. 2010;584:367-85 pubmed publisher
Goldstein R, Pomp O, Brokhman I, Ziegler L. Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells. Methods Mol Biol. 2010;584:283-300 pubmed publisher
Lin W, Metzakopian E, Mavromatakis Y, Gao N, Balaskas N, Sasaki H, et al. Foxa1 and Foxa2 function both upstream of and cooperatively with Lmx1a and Lmx1b in a feedforward loop promoting mesodiencephalic dopaminergic neuron development. Dev Biol. 2009;333:386-96 pubmed publisher
Du A, Hunter C, Murray J, Noble D, Cai C, Evans S, et al. Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas. Diabetes. 2009;58:2059-69 pubmed publisher
Bejarano Escobar R, Blasco M, DeGrip W, Martin Partido G, Francisco Morcillo J. Cell differentiation in the retina of an epibenthonic teleost, the Tench (Tinca tinca, Linneo 1758). Exp Eye Res. 2009;89:398-415 pubmed publisher
Ivaniutsin U, Chen Y, Mason J, Price D, Pratt T. Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex. Neural Dev. 2009;4:3 pubmed publisher
Ebert A, Yu J, Rose F, Mattis V, Lorson C, Thomson J, et al. Induced pluripotent stem cells from a spinal muscular atrophy patient. Nature. 2009;457:277-80 pubmed publisher
Yu T, Fotaki V, Mason J, Price D. Analysis of early ventral telencephalic defects in mice lacking functional Gli3 protein. J Comp Neurol. 2009;512:613-27 pubmed publisher
Reimer M, Sörensen I, Kuscha V, Frank R, Liu C, Becker C, et al. Motor neuron regeneration in adult zebrafish. J Neurosci. 2008;28:8510-6 pubmed publisher
Edqvist P, Lek M, Boije H, Lindbäck S, Hallböök F. Axon-bearing and axon-less horizontal cell subtypes are generated consecutively during chick retinal development from progenitors that are sensitive to follistatin. BMC Dev Biol. 2008;8:46 pubmed publisher
Ma Y, Song M, Park J, Henry Ho H, Hu L, Kurtev M, et al. Regulation of motor neuron specification by phosphorylation of neurogenin 2. Neuron. 2008;58:65-77 pubmed publisher
Stanke J, Lehman B, Fischer A. Muscarinic signaling influences the patterning and phenotype of cholinergic amacrine cells in the developing chick retina. BMC Dev Biol. 2008;8:13 pubmed publisher
Fischer A, Foster S, Scott M, Sherwood P. Transient expression of LIM-domain transcription factors is coincident with delayed maturation of photoreceptors in the chicken retina. J Comp Neurol. 2008;506:584-603 pubmed
Ellsworth B, Butts D, Camper S. Mechanisms underlying pituitary hypoplasia and failed cell specification in Lhx3-deficient mice. Dev Biol. 2008;313:118-29 pubmed
Feldner J, Reimer M, Schweitzer J, Wendik B, Meyer D, Becker T, et al. PlexinA3 restricts spinal exit points and branching of trunk motor nerves in embryonic zebrafish. J Neurosci. 2007;27:4978-83 pubmed
Raetzman L, Cai J, Camper S. Hes1 is required for pituitary growth and melanotrope specification. Dev Biol. 2007;304:455-66 pubmed
Davis S, Camper S. Noggin regulates Bmp4 activity during pituitary induction. Dev Biol. 2007;305:145-60 pubmed
Francisco Morcillo J, Hidalgo Sanchez M, Martin Partido G. Spatial and temporal patterns of proliferation and differentiation in the developing turtle eye. Brain Res. 2006;1103:32-48 pubmed
Kimura Y, Okamura Y, Higashijima S. alx, a zebrafish homolog of Chx10, marks ipsilateral descending excitatory interneurons that participate in the regulation of spinal locomotor circuits. J Neurosci. 2006;26:5684-97 pubmed
Ostendorff H, Tursun B, Cornils K, Schlüter A, Drung A, Güngör C, et al. Dynamic expression of LIM cofactors in the developing mouse neural tube. Dev Dyn. 2006;235:786-91 pubmed
Ikeda R, Kurokawa M, Chiba S, Yoshikawa H, Ide M, Tadokoro M, et al. Transplantation of neural cells derived from retinoic acid-treated cynomolgus monkey embryonic stem cells successfully improved motor function of hemiplegic mice with experimental brain injury. Neurobiol Dis. 2005;20:38-48 pubmed
Feldner J, Becker T, Goishi K, Schweitzer J, Lee P, Schachner M, et al. Neuropilin-1a is involved in trunk motor axon outgrowth in embryonic zebrafish. Dev Dyn. 2005;234:535-49 pubmed
Lacosta A, Muniesa P, Ruberte J, Sarasa M, Dominguez L. Novel expression patterns of Pax3/Pax7 in early trunk neural crest and its melanocyte and non-melanocyte lineages in amniote embryos. Pigment Cell Res. 2005;18:243-51 pubmed
Kato T, Ohtani Kaneko R, Ono K, Okado N, Shiga T. Developmental regulation of activated ERK expression in the spinal cord and dorsal root ganglion of the chick embryo. Neurosci Res. 2005;52:11-9 pubmed
Gonzalez Quevedo R, Shoffer M, Horng L, Oro A. Receptor tyrosine phosphatase-dependent cytoskeletal remodeling by the hedgehog-responsive gene MIM/BEG4. J Cell Biol. 2005;168:453-63 pubmed
Lewis K, Eisen J. Paraxial mesoderm specifies zebrafish primary motoneuron subtype identity. Development. 2004;131:891-902 pubmed
Kubo F, Takeichi M, Nakagawa S. Wnt2b controls retinal cell differentiation at the ciliary marginal zone. Development. 2003;130:587-98 pubmed
Becker T, Ostendorff H, Bossenz M, Schlüter A, Becker C, Peirano R, et al. Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development. Mech Dev. 2002;117:75-85 pubmed
Robb S, SANCHEZ ALVARADO A. Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy. Genesis. 2002;32:293-8 pubmed
Segawa H, Miyashita T, Hirate Y, Higashijima S, Chino N, Uyemura K, et al. Functional repression of Islet-2 by disruption of complex with Ldb impairs peripheral axonal outgrowth in embryonic zebrafish. Neuron. 2001;30:423-36 pubmed
Garces A, Haase G, Airaksinen M, Livet J, Filippi P, Delapeyriere O. GFRalpha 1 is required for development of distinct subpopulations of motoneuron. J Neurosci. 2000;20:4992-5000 pubmed
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Yamamoto Y, Henderson C. Patterns of programmed cell death in populations of developing spinal motoneurons in chicken, mouse, and rat. Dev Biol. 1999;214:60-71 pubmed
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Ericson J, Thor S, Edlund T, Jessell T, Yamada T. Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1. Science. 1992;256:1555-60 pubmed
product information
Internal ID :
4242
Name :
40.2D6
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
HHMI/Columbia University
Date Deposited :
8/4/93
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Islet-1 homeobox
Antigen Species :
rat
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Chicken,Fish,Frog,Human,Mouse,Newt,Planaria,Porcine,Rat,Reptile,Vertebrates,Xenopus,Zebrafish,altricial fish larva
Species Tested (legacy) :
all tested (rat, mouse, chick, frog, zebra fish and planaria) are positive
Initial Publication Pubmed ID :
1350865
Depositor Notes (Special Instructions) :
Reacted with all tested vertebrate species. The 40.2D6 monoclonal antibody is often combined with monoclonal antibodies 39.3F7, 39.4D5, and 40.3A4 to create a efficient cocktail for ChIP and/or gel shift [PMID 19502415].
Collections :
Cell markers,Human,Neurodevelopment,Transcription factors,Xenopus
Search Keywords :
Jessell, Thomas M / Brenner-Morton, Susan, Islet-1 homeobox, Rat, MIgG1, Vertebrates/Human/Rat/Mouse/Xenopus/Newt/Chicken/Frog/Zebrafish/Planaria/Reptile/Porcine, Isl1, Isl-1; isl-1=homeobox, AB_528315, monoclonal
Antigen Molecular Weight :
Predicted: 39kDa; Apparent: 39kDa
Gene :
Isl1
Alternate Gene Name(s) :
Isl-1; isl-1=homeobox
Uniprot ID :
P61374
Antibody Registry ID :
AB_528315
Immunogen :
C-terminal of rat Islet-1 (aa 178-349); recombinant .
Immunogen Sequence :
Recombinant C-terminal of rat Islet-1 (aa 178-349)
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
Yes
Epitope Map PubMed ID :
1350865 1764243
Recommended Applications :
Chromatin Immunoprecipitation,FACS,FFPE,Gel Supershift,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
24674670 24487799 24755910 25214396 25028525 24885223 19607821 12204249 11395004 10491257 16029418 16797493 16137565 21111494 19149881 18716209 17475806 16723525 16110501 14757641 19379735 20549713 31444329
Immunofluorescence Pubmed IDs :
24009262 24755910 25191843 24882712 25028525 24885223 24838392 24947469 24214099 24715462 21305616 20437528 21490060 21093585 21795538 22457492 16395690 19502415 19098894 12204249 18400164 10491257 10864957 11948917 17367776 17359964 18037398 18072193 22750943 19048639 16137565 21293464 22750882 23043799 22628072 23504940 12490564 15811548 9006983 20538044 10500113 19379735 20549713
Western Blot Pubmed IDs :
24674670 24214975 24055866 22628072
Chromatin Immunoprecipitation Pubmed IDs :
22194342 19502415
FFPE Pubmed IDs :
24009262 24674670 24755910 25214396 25028525 21490060 19502415 16797493 17367776
FACS Pubmed IDs :
22750943
Gel Supershift Pubmed IDs :
24674670 25028525 21388963
Pubmed IDs :
20702576 23284619 8755479 8978605 9915576 9890440 19907983 19907988 20018664 22457492 18716209 17475806 16723525 16110501 14757641
DSHB Growth Medium :
Iscove's
References (legacy) :
Science 256, 1555-1560.; Cell 73, 673-686.; Neuron 17, 63-74.; Cell 87, 1001-1014.; Devel. Dyn. 214, 55-65.; Dev. Biol. 214, 60-71.; J. Neurosci. Res. 55, 119-126.; J. Neurosci. 20(13), 4992-5000.; Genesis 32, 293-298.; Pigment Cell Res. 18(4), 243-251.; Brain Res. 1103, 32-48.; Dev. Biol. 304, 455-466.; Dev. Biol. 305, 145-160.; Dev. Biol. 313, 118-129.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US