beta-galactosidase | Developmental Studies Hybridoma Bank 40-1a product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

beta-galactosidase

catalog :

40-1a

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

40-1a

reactivity :

human, fruit fly, Escherichia coli str. K-12 substr. MG1655

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 61

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; fruit fly; 1:1000; loading ...; fig s3d	Zhou J, Edgar B, Boutros M. ATF3 acts as a rheostat to control JNK signalling during intestinal regeneration. Nat Commun. 2017;8:14289 pubmed publisher
immunohistochemistry; fruit fly; 1:50	Liu M, Li Y, Liu A, Li R, Su Y, Du J, et al. The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development. elife. 2016;5: pubmed publisher
western blot; human; 1:50; fig 1	Tang J, DROKHLYANSKY E, Etemad B, Rudolph S, Guo B, Wang S, et al. Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. elife. 2016;5: pubmed publisher
immunohistochemistry - paraffin section; fruit fly; 1:200; fig 1	Zappia M, Frolov M. E2F function in muscle growth is necessary and sufficient for viability in Drosophila. Nat Commun. 2016;7:10509 pubmed publisher
immunohistochemistry; fruit fly; 1:25; fig 4	Tognon E, Kobia F, Busi I, Fumagalli A, De Masi F, Vaccari T. Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in Drosophila melanogaster. Autophagy. 2016;12:499-514 pubmed publisher
immunohistochemistry; fruit fly; 1:200; loading ...; tbl 3	Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
immunohistochemistry; fruit fly; 1:150	Brown H, Reichert M, Evans T. Slit Binding via the Ig1 Domain Is Essential for Midline Repulsion by Drosophila Robo1 but Dispensable for Receptor Expression, Localization, and Regulation in Vivo. G3 (Bethesda). 2015;5:2429-39 pubmed publisher
immunohistochemistry; fruit fly; 1:50	Moris Sanz M, Estacio Gómez A, Alvarez Rivero J, Diaz Benjumea F. Specification of neuronal subtypes by different levels of Hunchback. Development. 2014;141:4366-74 pubmed publisher
	Li M, Sun S, Priest J, Bi X, Fan Y. Characterization of TNF-induced cell death in Drosophila reveals caspase- and JNK-dependent necrosis and its role in tumor suppression. Cell Death Dis. 2019;10:613 pubmed publisher
	Wolf D, Smylla T, Reichmuth J, Hoffmeister P, Kober L, Zimmermann M, et al. Nucleo-cytoplasmic shuttling of Drosophila Hairless/Su(H) heterodimer as a means of regulating Notch dependent transcription. Biochim Biophys Acta Mol Cell Res. 2019;1866:1520-1532 pubmed publisher
	Chang Y, Tu H, Chen J, Chang C, Yang S, Pi H. Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment. PLoS Genet. 2019;15:e1008062 pubmed publisher
	Jean Charles N, Buenaventura D, Emerson M. Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1. Neural Dev. 2018;13:26 pubmed publisher
	Pinal N, Martin M, Medina I, Morata G. Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis. Nat Commun. 2018;9:1541 pubmed publisher
	Forés M, Simón Carrasco L, Ajuria L, Samper N, González Crespo S, Drosten M, et al. A new mode of DNA binding distinguishes Capicua from other HMG-box factors and explains its mutation patterns in cancer. PLoS Genet. 2017;13:e1006622 pubmed publisher
	Fu C, Wang X, Cheng Q, Wang D, Hirose S, Liu Q. The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila. Sci Rep. 2016;6:27981 pubmed publisher
	Hao Y, Frey E, Yoon C, Wong H, Nestorovski D, Holzman L, et al. An evolutionarily conserved mechanism for cAMP elicited axonal regeneration involves direct activation of the dual leucine zipper kinase DLK. elife. 2016;5: pubmed publisher
	Le T, Vuong L, Kim A, Hsu Y, Choi K. 14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila. Nat Commun. 2016;7:11501 pubmed publisher
	Deshpande G, Manry D, Jourjine N, Mogila V, Mozes H, Bialistoky T, et al. Role of the ABC transporter Mdr49 in Hedgehog signaling and germ cell migration. Development. 2016;143:2111-20 pubmed publisher
	Slaninova V, Krafcikova M, Perez Gomez R, Steffal P, Trantirek L, Bray S, et al. Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle. Open Biol. 2016;6:150155 pubmed publisher
	Knapp J, Chung P, Simpson J. Generating customized transgene landing sites and multi-transgene arrays in Drosophila using phiC31 integrase. Genetics. 2015;199:919-34 pubmed publisher
	Lin T, Yeh T, Wang T, Yu J. The Hippo pathway controls border cell migration through distinct mechanisms in outer border cells and polar cells of the Drosophila ovary. Genetics. 2014;198:1087-99 pubmed publisher
	Ruas M, Chuang K, Davis L, Al Douri A, Tynan P, Tunn R, et al. TPC1 has two variant isoforms, and their removal has different effects on endo-lysosomal functions compared to loss of TPC2. Mol Cell Biol. 2014;34:3981-92 pubmed publisher
	Shimamura M, Kyotani A, Azuma Y, Yoshida H, Binh Nguyen T, Mizuta I, et al. Genetic link between Cabeza, a Drosophila homologue of Fused in Sarcoma (FUS), and the EGFR signaling pathway. Exp Cell Res. 2014;326:36-45 pubmed publisher
	Valakh V, Walker L, Skeath J, DiAntonio A. Loss of the spectraplakin short stop activates the DLK injury response pathway in Drosophila. J Neurosci. 2013;33:17863-73 pubmed publisher
	Chang Y, Jang A, Lin C, Montell D. Castor is required for Hedgehog-dependent cell-fate specification and follicle stem cell maintenance in Drosophila oogenesis. Proc Natl Acad Sci U S A. 2013;110:E1734-42 pubmed publisher
	Djiane A, Krejci A, Bernard F, Fexová S, Millen K, Bray S. Dissecting the mechanisms of Notch induced hyperplasia. EMBO J. 2013;32:60-71 pubmed publisher
	Brzezinski J, Prasov L, Glaser T. Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle. Dev Biol. 2012;365:395-413 pubmed publisher
	Eade K, Fancher H, Ridyard M, Allan D. Developmental transcriptional networks are required to maintain neuronal subtype identity in the mature nervous system. PLoS Genet. 2012;8:e1002501 pubmed publisher
	Andreu M, González Pérez E, Ajuria L, Samper N, González Crespo S, Campuzano S, et al. Mirror represses pipe expression in follicle cells to initiate dorsoventral axis formation in Drosophila. Development. 2012;139:1110-4 pubmed publisher
	Hongay C, Orr Weaver T. Drosophila Inducer of MEiosis 4 (IME4) is required for Notch signaling during oogenesis. Proc Natl Acad Sci U S A. 2011;108:14855-60 pubmed publisher
	Chen H, Wang C, Wang T, Liaw G, Hsu T, Lin T, et al. The Hippo pathway controls polar cell fate through Notch signaling during Drosophila oogenesis. Dev Biol. 2011;357:370-9 pubmed publisher
	Yu S, Yeh C, Niu Y, Chang H, Tsai Y, Moses H, et al. Altered prostate epithelial development in mice lacking the androgen receptor in stromal fibroblasts. Prostate. 2012;72:437-49 pubmed publisher
	Xiong X, Wang X, Ewanek R, Bhat P, DiAntonio A, Collins C. Protein turnover of the Wallenda/DLK kinase regulates a retrograde response to axonal injury. J Cell Biol. 2010;191:211-23 pubmed publisher
	Kitadate Y, Kobayashi S. Notch and Egfr signaling act antagonistically to regulate germ-line stem cell niche formation in Drosophila male embryonic gonads. Proc Natl Acad Sci U S A. 2010;107:14241-6 pubmed publisher
	Coleman H, Labrador J, Chance R, Bashaw G. The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline. Development. 2010;137:2417-26 pubmed publisher
	Sen A, Stultz B, Lee H, Hursh D. Odd paired transcriptional activation of decapentaplegic in the Drosophila eye/antennal disc is cell autonomous but indirect. Dev Biol. 2010;343:167-77 pubmed publisher
	Firth L, Bhattacharya A, Baker N. Cell cycle arrest by a gradient of Dpp signaling during Drosophila eye development. BMC Dev Biol. 2010;10:28 pubmed publisher
	Merino C, Penney J, González M, Tsurudome K, Moujahidine M, O Connor M, et al. Nemo kinase interacts with Mad to coordinate synaptic growth at the Drosophila neuromuscular junction. J Cell Biol. 2009;185:713-25 pubmed publisher
	Walker M, Brown R, Cronin T, Robinson P. Photochemistry of retinal chromophore in mouse melanopsin. Proc Natl Acad Sci U S A. 2008;105:8861-5 pubmed publisher
	BACHMANN A, Draga M, Grawe F, Knust E. On the role of the MAGUK proteins encoded by Drosophila varicose during embryonic and postembryonic development. BMC Dev Biol. 2008;8:55 pubmed publisher
	Garbe D, Bashaw G. Independent functions of Slit-Robo repulsion and Netrin-Frazzled attraction regulate axon crossing at the midline in Drosophila. J Neurosci. 2007;27:3584-92 pubmed
	Zhao L, Santiago A, Liu J, Liao D. Repression of p53-mediated transcription by adenovirus E1B 55-kDa does not require corepressor mSin3A and histone deacetylases. J Biol Chem. 2007;282:7001-10 pubmed
	Geng C, Macdonald P. Imp associates with squid and Hrp48 and contributes to localized expression of gurken in the oocyte. Mol Cell Biol. 2006;26:9508-16 pubmed
	Madsen J, Stoltzfus C. An exonic splicing silencer downstream of the 3' splice site A2 is required for efficient human immunodeficiency virus type 1 replication. J Virol. 2005;79:10478-86 pubmed
	Cáceres L, Nilson L. Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte. Development. 2005;132:2345-53 pubmed
	Kadrmas J, Smith M, Clark K, Pronovost S, Muster N, Yates J, et al. The integrin effector PINCH regulates JNK activity and epithelial migration in concert with Ras suppressor 1. J Cell Biol. 2004;167:1019-24 pubmed
	Bieberich E, Silva J, Wang G, Krishnamurthy K, Condie B. Selective apoptosis of pluripotent mouse and human stem cells by novel ceramide analogues prevents teratoma formation and enriches for neural precursors in ES cell-derived neural transplants. J Cell Biol. 2004;167:723-34 pubmed
	Rukstalis J, Kowalik A, Zhu L, Lidington D, Pin C, Konieczny S. Exocrine specific expression of Connexin32 is dependent on the basic helix-loop-helix transcription factor Mist1. J Cell Sci. 2003;116:3315-25 pubmed
	Li Y, Lei L, Irvine K, Baker N, Li L. Notch activity in neural cells triggered by a mutant allele with altered glycosylation. Development. 2003;130:2829-40 pubmed
	Li Y, Fetchko M, Lai Z, Baker N. Scabrous and Gp150 are endosomal proteins that regulate Notch activity. Development. 2003;130:2819-27 pubmed
	Yang L, Baker N. Cell cycle withdrawal, progression, and cell survival regulation by EGFR and its effectors in the differentiating Drosophila eye. Dev Cell. 2003;4:359-69 pubmed
	Li Y, Baker N. Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye. Curr Biol. 2001;11:330-8 pubmed
	Baker N, Yu S. The EGF receptor defines domains of cell cycle progression and survival to regulate cell number in the developing Drosophila eye. Cell. 2001;104:699-708 pubmed
	Yang L, Baker N. Role of the EGFR/Ras/Raf pathway in specification of photoreceptor cells in the Drosophila retina. Development. 2001;128:1183-91 pubmed
	Donahue C, Carruthers V, Gilk S, Ward G. The Toxoplasma homolog of Plasmodium apical membrane antigen-1 (AMA-1) is a microneme protein secreted in response to elevated intracellular calcium levels. Mol Biochem Parasitol. 2000;111:15-30 pubmed
	Lee E, Yu S, Baker N. The scabrous protein can act as an extracellular antagonist of notch signaling in the Drosophila wing. Curr Biol. 2000;10:931-4 pubmed
	Goto S, Hayashi S. Proximal to distal cell communication in the Drosophila leg provides a basis for an intercalary mechanism of limb patterning. Development. 1999;126:3407-13 pubmed
	Lesokhin A, Yu S, Katz J, Baker N. Several levels of EGF receptor signaling during photoreceptor specification in wild-type, Ellipse, and null mutant Drosophila. Dev Biol. 1999;205:129-44 pubmed
	Parsons M, Nelson R, Agabian N. The trypanosome spliced leader small RNA gene family: stage-specific modification of one of several similar dispersed genes. Nucleic Acids Res. 1986;14:1703-18 pubmed
	Privalsky M. A subpopulation of the v-erb A oncogene protein, a derivative of a thyroid hormone receptor, associates with heat shock protein 90. J Biol Chem. 1991;266:1456-62 pubmed
	Ghattas I, Sanes J, Majors J. The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos. Mol Cell Biol. 1991;11:5848-59 pubmed

product information

Internal ID :

2006

Name :

40-1a

Depositor Name :

Sanes, J.R.

Depositor Institution :

Harvard University

Date Deposited :

7/22/92

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

beta-galactosidase

Antigen Species :

E. coli

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

E. coli

Initial Publication Pubmed ID :

1658618

Depositor Notes (Special Instructions) :

Recognizes the E.coli beta-galatosidase sequence in beta-gal fusion proteins.

Collections :

Enzymes,Epitope tags,Microbe antibodies,Protein tags

Search Keywords :

Sanes, Joshua R., beta-galactosidase, E. coli, MIgG1, E. coli, LacZ, , AB_2314509, monoclonal

Antigen Molecular Weight :

Predicted: 116 kDa

Gene :

LacZ

Uniprot ID :

P00722

Antibody Registry ID :

AB_2314509

Immunogen :

Beta-galactosidase (E.coli)

Clonality :

Monoclonal

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

10959842 20403347 31326540 31409797

Immunofluorescence Pubmed IDs :

1658618 10393119 9882502 11245584 11267869 12829745 12756167 12756168 15829517 22445509 17030623 29670104 27151460 27122170 30466480 31295251

Western Blot Pubmed IDs :

12829745 25135478 11087913 16051840

Pubmed IDs :

11257224 12636917

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 126, 3407-3413.; Dev. Biol. 205, 129-144.; Curr. Biol. 10, 931-934.; Development 128, 1183-1191.; Curr. Biol. 11, 330-338.; Cell 104, 699-708.; J. Cell Sci. 116, 3315-3325.; Development 130, 2819-2827.; Development 130, 2829-2840.; Dev. Cell 4, 359-369.; Development 132, 2345-2353.; Dev. Biol. 343, 167-177.; Dev. Biol. 365, 395-413.; Mol. Cell. Biol. doi:10.1128/MCB.00113-14.

more info or order :

Developmental Studies Hybridoma Bank product webpage