product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Synaptotagmin
catalog :
3H2 2D7
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3H2 2D7
reactivity :
mouse, Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 17
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; Drosophila melanogaster; 1:100
Li L, Tian X, Zhu M, Bulgari D, Böhme M, Goettfert F, et al. Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons. J Neurosci. 2014;34:8474-87 pubmed publisher
Khan A, Paro S, McGurk L, Sambrani N, Hogg M, Brindle J, et al. Membrane and synaptic defects leading to neurodegeneration in Adar mutant Drosophila are rescued by increased autophagy. BMC Biol. 2020;18:15 pubmed publisher
Furotani K, Kamimura K, Yajima T, Nakayama M, Enomoto R, Tamura T, et al. Suppression of the synaptic localization of a subset of proteins including APP partially ameliorates phenotypes of the Drosophila Alzheimer's disease model. PLoS ONE. 2018;13:e0204048 pubmed publisher
Koon A, Chen Z, Peng S, Fung J, Zhang X, Lembke K, et al. Drosophila Exo70 Is Essential for Neurite Extension and Survival under Thermal Stress. J Neurosci. 2018;38:8071-8086 pubmed publisher
Neuman S, Bashirullah A. Hobbit regulates intracellular trafficking to drive insulin-dependent growth during Drosophila development. Development. 2018;145: pubmed publisher
Jin E, Kiral F, Ozel M, Burchardt L, Osterland M, Epstein D, et al. Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals. Curr Biol. 2018;28:1027-1038.e4 pubmed publisher
Furukubo Tokunaga K, Kurita K, Honjo K, Pandey H, Ando T, Takayama K, et al. DISC1 causes associative memory and neurodevelopmental defects in fruit flies. Mol Psychiatry. 2016;21:1232-43 pubmed publisher
Coyle I. Confocal imaging of fluorescently labeled proteins in the Drosophila larval neuromuscular junction. Methods Mol Biol. 2014;1075:201-12 pubmed publisher
Kim M, Park H, Park H, Ro S, Nam S, Reed J, et al. Drosophila Fip200 is an essential regulator of autophagy that attenuates both growth and aging. Autophagy. 2013;9:1201-13 pubmed publisher
Hourai A, Miyata S. Neurogenesis in the circumventricular organs of adult mouse brains. J Neurosci Res. 2013;91:757-70 pubmed publisher
Stevens R, Akbergenova Y, Jorquera R, Littleton J. Abnormal synaptic vesicle biogenesis in Drosophila synaptogyrin mutants. J Neurosci. 2012;32:18054-67, 18067a pubmed publisher
Ikeno H, Kazawa T, Namiki S, Miyamoto D, Sato Y, Haupt S, et al. Development of a scheme and tools to construct a standard moth brain for neural network simulations. Comput Intell Neurosci. 2012;2012:795291 pubmed publisher
Beck E, Gasque G, Imlach W, Jiao W, Jiwon Choi B, Wu P, et al. Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag. J Neurosci. 2012;32:7058-73 pubmed publisher
Johnson E, Fetter R, Davis G. Negative regulation of active zone assembly by a newly identified SR protein kinase. PLoS Biol. 2009;7:e1000193 pubmed publisher
Fukushima R, Kanzaki R. Modular subdivision of mushroom bodies by Kenyon cells in the silkmoth. J Comp Neurol. 2009;513:315-30 pubmed publisher
Menon K, Sanyal S, Habara Y, Sanchez R, Wharton R, Ramaswami M, et al. The translational repressor Pumilio regulates presynaptic morphology and controls postsynaptic accumulation of translation factor eIF-4E. Neuron. 2004;44:663-76 pubmed
Dubuque S, Schachtner J, Nighorn A, Menon K, Zinn K, Tolbert L. Immunolocalization of synaptotagmin for the study of synapses in the developing antennal lobe of Manduca sexta. J Comp Neurol. 2001;441:277-87 pubmed
product information
Internal ID :
1214
Name :
3H2 2D7
Depositor Name :
Zinn, K.
Depositor Institution :
California Institute of Technology
Date Deposited :
3/16/04
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Synaptotagmin
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Crayfish,Drosophila,Insect,Mouse
Species Tested (legacy) :
Drosophila, Manduca sexta, Procamrius clarkia (crayfish)
Initial Publication Pubmed ID :
11745650
Depositor Notes (Special Instructions) :
Insect species that tested positive include Manduca sexta (tobacco hornworm), Periplaneta americana (cockroach), Orthoptera sp. (locust), and Bombyx mori (silkworm moth) Crustaceans include the freshwater crayfish.
Collections :
Cell markers,Drosophila antigens,Neurodevelopment
Search Keywords :
Zinn, Kai, Synaptotagmin, Drosophila, MIgG1, kappa light chain, Mouse/Drosophila/Insect/crayfish, Syt1, Droso1; DSypt; dSYT; DSYT; dsyt1; Dsyt2; SYT1; , AB_528483, monoclonal
Antigen Molecular Weight :
Predicted: multiple isoforms at 19, 37, and 53kDa
Gene :
Syt1
Alternate Gene Name(s) :
Droso1; DSypt; dSYT; DSYT; dsyt1; Dsyt2; SYT1;
Uniprot ID :
P21521
Antibody Registry ID :
AB_528483
Immunogen :
Synaptotagmin recombinant, purified.
Immunogen Sequence :
Recombinant synaptotagmin, Partial protein (= conserved cytoplasmic domain, amino acids 134-474)
Clonality :
Monoclonal
Myeloma Strain :
HL-1
Epitope Mapped :
Yes
Epitope Map PubMed ID :
11745650
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
11745650
Immunofluorescence Pubmed IDs :
11745650 23819996 19148932 22952471 23526379 22593074 15541314 19771148 23238721
Western Blot Pubmed IDs :
11745650
Pubmed IDs :
24052353
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Comp. Neurol 441, 277-287.; J. Comp. Neurol. 513, 315-330.; P. Neurosci. 12, 1-5.; J. Neurosci. 32(20), 7058-7073.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US